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Firdaus, Muhammad Lutfi
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Chemistry

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Box-Behnken Experimental Design for Electrochemical Aptasensor Optimization on Screen Printed Carbon Electrode/Silica-Ceria Zakiyyah, Salma Nur; Eddy, Diana Rakhmawaty; Firdaus, Muhammad Lutfi; Subroto, Toto; Hartati, Yeni Wahyuni
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 1, May 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i1.27493

Abstract

This study aims to optimize the epithelial sodium channel (ENaC) electrochemical aptasensor with the Box-Behnken experimental design. ENaC is a protein that plays a role in sodium ion transport in several epithelial tissues and is associated with hypertension. The ENaC protein aptamer is held in place in the electrochemical aptasensor by a modified screen-printed carbon electrode (SPCE) of silica-ceria composite (SiO2-CeO2). The unique structure of a silica matrix with high biocompatibility can form composites through a hydrothermal process. The Box-Behnken (BBD) experimental design is an efficient optimization method of factors that affect the experiment at three levels. The FTIR results of the silica-ceria composites were 549.35 cm-1 (Ce-O), 1095.3 cm-1 (Si-O-Si), and 491.28 cm-1 (Si-O). Meanwhile, SPCE/silica-ceria characterized by differential pulse voltammetry (DPV) showed an increase in peak current [Fe(CN)6]3-/4- from 3.190 μA to 9.073 μA. Three experimental factors, aptamer concentration, streptavidin incubation time, and aptamer incubation time, were optimized with BBD and obtained at 0.5 μg.mL-1, 30 minutes, and 1 hour. The optimum conditions observed resulted in a selective current response for ENaC protein detection. The optimization results can be applied to aptamer-based ENaC protein detection in samples.
Co-Authors Eddy, Diana Rakhmawaty Toto Subroto Yeni Wahyuni Hartati Zakiyyah, Salma Nur