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Lateral-flow immunoassay as a diagnostic test for influenza type A and B in children Ity Sulawati; Amalia Setyati; A. Samik Wahab; M. Juffrie
Paediatrica Indonesiana Vol 48 No 2 (2008): March 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.874 KB) | DOI: 10.14238/pi48.2.2008.104-9

Abstract

Background The diagnosis of influenza remains difficult toestablish because of its similar symptoms to those of respiratoryinfection caused by other viruses. The “gold standard” for thediagnosis of influenza is viral culture, which takes time to gainthe result and is expensive as well. A simple, rapid, and easilyused tool for detection of influenza virus type A and B is needed.Objective To assess the accuracy of lateral-flow immunoassay withQuick Vue Influenza A+B ® in detecting influenza virus of typeA and B.Methods This was an observational study designed for diagnostictest. The subjects were children aged 0-14 years old presentingwith acute respiratory infection in primary Health Care Jetis ,Godean I, Godean II and Prof. Dr. Sardjito Hospital Yogyakarta,from October 2005 to May 2007. Specimens were collected fromboth the anterior nares and the throat by physicians for lateral-flow immunoassay with Quick Vue Influenza A+B ® and viralculture as gold standard. Lateral-flow immunoassay was done ineach study centre, nasal specimen was placed in an extractionreagent tube and sent to NAMRU II laboratory.Results There were 255 children enrolled in this study. Lateral-flow immunoassay by Quick Vue Influenza A+B ® has sensitivity70% (CI95% 6;83%), specificity 93% (CI95% 90;97%), positivepredictive value 68% (CI95% 54;82%), negative predictive value94% (CI95% 91;97%), positive likelihood ratio 10,56 (CI95%6,14;18,19) and negative likelihood ratio 0,32 (CI95% 0,21; 0,51).Conclusion Lateral-flow immunoassay (Quick Vue InfluenzaA+B ® ), nasal swab specimen is not accurate to detect influenzavirus A and B in children.
Effectiveness of synbiotics as laxative agent for constipation in children Hannah Hannah; M. Juffrie; S. Yati Soenarto
Paediatrica Indonesiana Vol 48 No 3 (2008): May 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.084 KB) | DOI: 10.14238/pi48.3.2008.136-41

Abstract

Background Constipation in children can cause serious problems.Laxative is needed for the management of constipation. Orallaxative has been used frequently, even though a safe oral laxativeagents for children is limited. Prebiotic and probiotic have alreadybeen proven to have laxative effect in constipation in children andadults. The effectiveness of these agents as a laxative has not beenproven yet.Objective To evaluate the effectiveness of laxative synbiotic inconstipation in children compared to that of fiber foods.Methods A randomized double blind controlled trial wasconducted on children aged 6 months-14 years old who sufferedfrom functional constipation at Dr. Sardjito Hospital, Dr. SoeradjiTirtonegoro Hospital, and Wates District Hospital from April2007-October 2007. Randomization was performed by computer. Theoutcome of recovery rate, onset therapy and side effects wereevaluated after seven days of intervention.Results Forty-three children were included in this study, but only41 could be analyzed. Subject characteristics in both groups werecomparable except for maternal educational level. The mainoutcome (recovery rate) was assessed by intention to treat principle.Compared with fiber foods, synbiotic increased the recovery ratewith RR 2.14 (95%CI 1.14; 4.02) and NNT 2.9 (95%CI 2; 15),whereas its therapeutic onset was 15 hours faster than that of fiberfoods. No important side effects were found in both groups.Conclusions Synbiotic is safe and effective in increasing therecovery rate of functional constipation with faster therapeuticonset than that of fiber foods.
Reverse Transcription Polymerase Chain Reaction (RT-PCR) as an influenza diagnostic test among children in Yogyakarta Eva Musdalifah; M. Juffrie; Purnomo S; Amalia Amalia
Paediatrica Indonesiana Vol 48 No 5 (2008): September 2008
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.731 KB) | DOI: 10.14238/pi48.5.2008.288-91

Abstract

Background Influenza virus type A, especially H5Nl subtype oravian influenza, is a highly pathogenic agent that causes epidemicin the world with high mortality. Most cases are preschool andschool children. Anti-viral drug is effective when given at earlyphase. The gold standard for the diagnosis of influenza is viralculture, which takes 2 to 10 days. A rapid and accurate diagnostictest is needed to control further viral infection.Objective To determine the accuracy of RT-PCR as a diagnostictest for children with influenza compared with viral culture.Methods A cross-sectional study was conducted in primary healthcares of Jetis I, Godean I, II and Dr Sardjito Hospital Yogyakartabetween January 2005 and May 2007. The specimens, taken bytrained health personnel, were collected from both anterior naresand throat of children aged from birth to 14 years who met theeligibility criteria, then were stored in a frozen extraction tubeand sent to Jakarta for RT-PCR and viral culture as the goldstandard.Results There were 34 7 children enrolled in this study. Influenzainfection was confirmed in 63 children (18.2%). There were 24children with H3N2 subtype of influenza virus, 13 children withHINI subtype, and one child with H5Nl subtype. The sensitivity,specificity, positive and negative predictive value, positive andnegative likelihood ratio of RT-PCR test were 89%, 90%, 67%,97%, 9, 3, 0, and 12 respectively.Conclusions RT-PCR is accurate enough as influenza diagnostictest in children.
Suplementasi obat cacing, sirup Fe, dan vitamin C meningkatkankadar hemoglobin dan status gizi balita di Kupang Siti Romlah; Hamam Hadi; M. Juffrie
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 1, NOMOR 1, JANUARI 2013
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.778 KB) | DOI: 10.21927/ijnd.2013.1(1).1-6

Abstract

ABSTRACTBackground: The prevalence of having intestinal worms among malnourished children under fi ve as the cause of anemia at the Province of Nusa Tenggara Timur is still relatively high. Efforts to minimize intestinal worm infection and anemia and to increase nutrition status of malnourished children under fi ve who get recovery complementary foods are made through supply of intestinal worm drugs, Fe syrup and vitamin C.Objective: To identify the effect of the supply of intestinal worm drugs, Fe syrup, and vitamin C supplementation to the increase of haemoglobin (Hb) level and nutritional status of malnourished children under fi ve who got recovery complementary foods.Method: This was an experimental study with factorial design. Subject of the study were malnourished children under fi ve of 1–3 years of age at Kupang Municipality. There were as many as 128 samples divided into 4 experiment groups. The group got pyrantel pamoat intestinal worm drugs 125 mg (n=32), Fe syrup + vitamin C (n=32), and placebo (n=32). Hb level was measured using “Hemocue-B Hemoglobin photometer”, intestinal worm infection was observed through facces of the subject to identify the presence of worm eggs. Statistical analysis used t-test to identify the relationship before and after the supply and ANOVA to fi nd out the difference in the effect of the supply among the groups. Result: After 3 months, the supply of the intestinal worm drugs before intervention had signifi cant effect to intestinal worm infection status among the group with intestinal worm drugs and the group with intestinal worm drugs, Fe syrup + vitamin C. The highest increase of Hb level was found in the group with intestinal worm drugs, Fe syrup + Vitamin C with average increase as much as 1.2 g/dL. Average increase of weight among the groups was 0.3 kg. Increase ofZ-score signifi cantly affected index of weight/age and weight/height.Conclusion: The increase of Hb level signifi cantly affected changes of nutritional status increase among the experiment groups.KEYWORDS: intestinal worm drugs, Fe syrup, vitamin C, Hb level, nutritional status, malnourishmentABSTRAKLatar Belakang: Prevalensi kecacingan pada balita gizi buruk sebagai penyebab anemia di Provinsi NTT masih tinggi. Upaya menurunkan infeksi kecacingan dan anemia serta meningkatkan status gizi pada balita gizi buruk yang mendapat PMT-P antara lain dengan pemberian obat cacing dan suplementasi sirup Fe + vitamin C.Tujuan: Mengetahui pengaruh pemberian obat cacing, suplemen sirup Fe, dan vitamin C terhadap peningkatan kadar Hb dan status gizi balita gizi buruk penerima PMT-P.Metode: Jenis penelitian adalah eksperimental dengan rancangan faktorial. Sasaran penelitian adalah balita gizi buruk usia 1–3 tahun di Kota Kupang. Jumlah sampel sebanyak 128 anak dibagi dalam 4 kelompok perlakuan. Perlakuan obat cacing pirantel pamoat 125 mg (n=32), sirup Fe + vitamin C (n=32), obat cacing, sirup Fe+ vitamin C (n=32) dan plasebo (n=32). Pengukuran kadar hemoglobin dengan ”HemoCue”, infeksi cacing diperiksa melalui tinja subjek untuk melihat adanya telur cacing. Analisis menggunakan uji T-test untuk mengetahui hubungan sebelum dan sesudah suplementasi dan uji ANOVA untuk melihat perbedaan efek suplementasi antar kelompok. Hasil: Setelah 3 bulan, pemberian obat cacing sebelum intervensi berpengaruh signifi kan terhadap status infeksi kecacingan pada kelompok obat cacing dan kelompok obat cacing, sirup Fe + vitamin C. Peningkatan kadar hemoglobin antarkelompok yang tertinggi adalah pada kelompok obat cacing, sirup Fe dan vitamin C dengan ratarata kenaikan sebesar 1,2 g/dL. Rata-rata kenaikan berat badan antar kelompok sebesar 0,3 kg. Peningkatan nilai Z-score berpengaruh signifi kan terhadap indek BB/U dan BB/TB.Kesimpulan:Peningkatkan kadar hemoglobin berpengaruh signifikan terhadap perubahan peningkatan status gizi antar kelompok suplementasi.KATA KUNCI: obat cacing, sirup Fe +vitamin C, kadar hemoglobin, Z-score, status gizi