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All Journal Paediatrica Indonesiana Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics)

M. Juffrie

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Author-ID : 1965432

Health Professions Medicine & Pharmacology Public Health Other

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Lateral-flow immunoassay as a diagnostic test for influenza type A and B in children Ity Sulawati; Amalia Setyati; A. Samik Wahab; M. Juffrie
Paediatrica Indonesiana Vol 48 No 2 (2008): March 2008
Publisher : Indonesian Pediatric Society

Background The diagnosis of influenza remains difficult toestablish because of its similar symptoms to those of respiratoryinfection caused by other viruses. The â€œgold standardâ€ for thediagnosis of influenza is viral culture, which takes time to gainthe result and is expensive as well. A simple, rapid, and easilyused tool for detection of influenza virus type A and B is needed.Objective To assess the accuracy of lateral-flow immunoassay withQuick Vue Influenza A+B Â® in detecting influenza virus of typeA and B.Methods This was an observational study designed for diagnostictest. The subjects were children aged 0-14 years old presentingwith acute respiratory infection in primary Health Care Jetis ,Godean I, Godean II and Prof. Dr. Sardjito Hospital Yogyakarta,from October 2005 to May 2007. Specimens were collected fromboth the anterior nares and the throat by physicians for lateral-flow immunoassay with Quick Vue Influenza A+B Â® and viralculture as gold standard. Lateral-flow immunoassay was done ineach study centre, nasal specimen was placed in an extractionreagent tube and sent to NAMRU II laboratory.Results There were 255 children enrolled in this study. Lateral-flow immunoassay by Quick Vue Influenza A+B Â® has sensitivity70% (CI95% 6;83%), specificity 93% (CI95% 90;97%), positivepredictive value 68% (CI95% 54;82%), negative predictive value94% (CI95% 91;97%), positive likelihood ratio 10,56 (CI95%6,14;18,19) and negative likelihood ratio 0,32 (CI95% 0,21; 0,51).Conclusion Lateral-flow immunoassay (Quick Vue InfluenzaA+B Â® ), nasal swab specimen is not accurate to detect influenzavirus A and B in children.

Effectiveness of synbiotics as laxative agent for constipation in children Hannah Hannah; M. Juffrie; S. Yati Soenarto
Paediatrica Indonesiana Vol 48 No 3 (2008): May 2008
Publisher : Indonesian Pediatric Society

Background Constipation in children can cause serious problems.Laxative is needed for the management of constipation. Orallaxative has been used frequently, even though a safe oral laxativeagents for children is limited. Prebiotic and probiotic have alreadybeen proven to have laxative effect in constipation in children andadults. The effectiveness of these agents as a laxative has not beenproven yet.Objective To evaluate the effectiveness of laxative synbiotic inconstipation in children compared to that of fiber foods.Methods A randomized double blind controlled trial wasconducted on children aged 6 months-14 years old who sufferedfrom functional constipation at Dr. Sardjito Hospital, Dr. SoeradjiTirtonegoro Hospital, and Wates District Hospital from April2007-October 2007. Randomization was performed by computer. Theoutcome of recovery rate, onset therapy and side effects wereevaluated after seven days of intervention.Results Forty-three children were included in this study, but only41 could be analyzed. Subject characteristics in both groups werecomparable except for maternal educational level. The mainoutcome (recovery rate) was assessed by intention to treat principle.Compared with fiber foods, synbiotic increased the recovery ratewith RR 2.14 (95%CI 1.14; 4.02) and NNT 2.9 (95%CI 2; 15),whereas its therapeutic onset was 15 hours faster than that of fiberfoods. No important side effects were found in both groups.Conclusions Synbiotic is safe and effective in increasing therecovery rate of functional constipation with faster therapeuticonset than that of fiber foods.

Reverse Transcription Polymerase Chain Reaction (RT-PCR) as an influenza diagnostic test among children in Yogyakarta Eva Musdalifah; M. Juffrie; Purnomo S; Amalia Amalia
Paediatrica Indonesiana Vol 48 No 5 (2008): September 2008
Publisher : Indonesian Pediatric Society

Background Influenza virus type A, especially H5Nl subtype oravian influenza, is a highly pathogenic agent that causes epidemicin the world with high mortality. Most cases are preschool andschool children. Anti-viral drug is effective when given at earlyphase. The gold standard for the diagnosis of influenza is viralculture, which takes 2 to 10 days. A rapid and accurate diagnostictest is needed to control further viral infection.Objective To determine the accuracy of RT-PCR as a diagnostictest for children with influenza compared with viral culture.Methods A cross-sectional study was conducted in primary healthcares of Jetis I, Godean I, II and Dr Sardjito Hospital Yogyakartabetween January 2005 and May 2007. The specimens, taken bytrained health personnel, were collected from both anterior naresand throat of children aged from birth to 14 years who met theeligibility criteria, then were stored in a frozen extraction tubeand sent to Jakarta for RT-PCR and viral culture as the goldstandard.Results There were 34 7 children enrolled in this study. Influenzainfection was confirmed in 63 children (18.2%). There were 24children with H3N2 subtype of influenza virus, 13 children withHINI subtype, and one child with H5Nl subtype. The sensitivity,specificity, positive and negative predictive value, positive andnegative likelihood ratio of RT-PCR test were 89%, 90%, 67%,97%, 9, 3, 0, and 12 respectively.Conclusions RT-PCR is accurate enough as influenza diagnostictest in children.

Co-Authors A. Samik Wahab Amalia Amalia Amalia Setyati Eva Musdalifah Hamam Hadi Hannah Hannah Ity Sulawati Purnomo S Siti Romlah Yati Soenarto

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