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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Ternak Tropika JURNAL ILMIAH PETERNAKAN TERPADU ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal JURNAL KAJIAN VETERINER Journal of Applied Veterinary Science and Technology Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
Haskito, Ajeng Erika Prihastuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Pengaruh Pemberian Tablet Konvensional Kasein Yogurt Susu Kambing Terhadap Akumulasi Residu Dioksin dan Kerusakan Jaringan Musculus Pectoralis Pada Ayam Broiler Yang Dipapar Dioksin Anjani, Godelva Permata; Haskito, Ajeng Erika Prihastuti; Roosdiana, Anna; Pratiwi, Herlina; Mahdi, Chanif
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 23 No. 1 (2022): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2022.023.01.10

Abstract

Dioksin merupakan turunan dari Polychlorinated trichloro-dibenzo-p-dioxins (TCDDs) yang bersifat lipofilik dan bioakumulasi. Dioksin mampu berikatan dengan Aryl hydrocarbon Receptors maupun Transforming Growth Faktor Beta, sehingga sitokrom P450 teraktivasi dan memicu kerusakan jaringan. Kasein yogurt susu kambing merupakan sumber peptida bioaktif dan antioksidan. Penelitian ini bertujuan untuk melihat pengaruh tablet konvensional kasein yogurt susu kambing terhadap kadar residu dioksin dan kerusakan jaringan musuculus pectoralis ayam broiler yang terpapar dioksin. Penelitian ini menggunakan 18 ekor ayam broiler berusia 21 hari, dibagi menjadi 3 kelompok, terdiri atas: 1) Kelompok negatif (tanpa perlakukan); 2) Kelompok positif (TCDD 50 ng/kg pakan/hari); 3) Kelompok terapi (TCDD 50 ng/kg pakan/hari + tablet konvensional kasein yogurt susu kambing 750 mg/ekor peroral). Kadar residu dioksin diukur dengan metode spektrofotometri-UV diolah sebagai data kuantitatif dianalisa dengan sidik ragam (ANOVA) dilanjutkan Uji Tukey. Histopatologi musculus pectoralis diwarnai dengan menggunakan metode pewarnaan Hematoxylin-Eosin (HE) dan diamati secara deskriptif. Hasil penelitian menunjukkan analisa statistik pemberian tablet konvensional kasein yogurt susu kambing memberikan pengaruh tidak berbeda nyata (p>0.05) pada penurunan kadar residu dioksin, namun data pengukuran residu dioksin menunjukkan bahwa adanya penurunan kadar residu dioksin sebesar 18,20%. Pemberian tablet konvensional kasein yogurt susu kambing juga terbukti dapat memperbaiki kerusakan jaringan musculus pectoralis ayam broiler (Gallus domesticus) yang dipapar dioksin dengan mengurangi jumlah jaringan ikat perimisium yang mengalami penebalan. Kesimpulan penelitian ini adalah pemberian tablet konvensional kasein yogurt susu kambing tidak memberi pengaruh yang signifikan untuk menurunkan kadar residu TCDD dan dapat memperbaiki kerusakan histopatologi musculus pectoralis ayam broiler (Gallus domesticus) yang dipapar dioksin.
Acidity and Alcohol Levels of Goat’s Milk Yoghurt with the Addition of Black Rice Bran Flour During Cold Storage Haskito, Ajeng Erika Prihastuti; Fatra, Ilhami Maulia; Mahdi, Chanif; Noviatri, Aldila; Adrenalin, Sruti Listra
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p354-366

Abstract

The development of yoghurt variations is increasingly diverse nowadays. The addition of black rice bran flour into yoghurt processing can increase the functional value of yoghurt, including increasing taste, antioxidant content, and dietary fiber. This research was to determine the effect of storage time in goat's milk yoghurt with the addition of black rice bran flour at a cold temperature of 4c on acidity and alcohol levels. The research used with 5 treatments, 4 repetitions. The treatment includes storage periods  i.e., 0, 1, 2, 3 and 4 weeks. The acidity level will be calculated for each treatment group using the acid-base and alcohol titration method using the pycnometer method. The data obtained were analyzed using the one way ANOVA test followed by the Duncan Multiple Range Test. The results show that storage time has a very significant effect (p˂0.01) on acidity and alcohol levels. The highest acidity level was in week 2 (0.47%), then decreased continuously in weeks 3 and 4 of storage time. The alcohol content increased in line with storage time, reaching 0.0043% in week 4. If  we look at the acidity and alcohol levels, the recommendation for consuming goat's milk yoghurt with the addition of black rice bran flour is no more than 1 weeks of storage time.
Preventive effects of yogurt fortified with purple roselle extract against cardiotoxicity in rats exposed with 2,3,7,8-tetrachlorodibenzo-p-dioxin Setianingrum, Ani; Fanadila, Iffa; Pratiwi, Herlina; Haskito, Ajeng Erika Prihastuti
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.02

Abstract

Dioxin is one of the environmental persistent organic pollutants that harm animal and human health. Prolonged exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can disrupt normal organ function including heart. Yogurt is known to have antioxidant properties, and fortification natural plant-based antioxidant purple roselle (Hibiscus sabdariffa L) extract into yogurt can improve the antioxidant potential. The purpose of this study was to determine the preventive effect of yogurt fortified with purple roselle on dioxin exposure rats (Rattus norvegicus) based on the levels of Malondialdehyde (MDA) and histopathological of the heart. This experimental study using a completely randomized design (CRD). A total of 30 male Wistar rats were divided into 5 groups (NC, PC, T1, T2, T3), a dose of 200 ng/kg BW TCDD and 1 mL of roselle yogurt 0.5%, 1.0%, and 1.5% were given orally for 12 days. MDA levels were analyzed using the Thiobarbituric Acid (TBA) assay and histopathology of the heart using the Haematoxylin Eosin (HE) staining method. Statistical analysis using one-way Analysis of Variance (ANOVA). Histopathology changes were analyzed descriptively. The results showed that TCDD exposure induced oxidative stress in heart tissue and yogurt roselle has the potential to prevent an increase of MDA levels significantly (p<0.05). Furthermore, TCDD can cause histopathological alterations such as necrosis and hemorrhage, and yogurt roselle was proven to prevent cardiotoxicity due to TCDD exposure.
Co-Authors Adrenalin, Sruti Listra Anjani, Godelva Permata Anna Roosdiana Chanif Mahdi Citra Sari Dameanti, Fidi Nur Aini Eka Puji Fanadila, Iffa Fatmawati, Mira Fatra, Ilhami Maulia Immauliate, Sinthia Medchera Inayah, Khoirul Jangnga, Indah Puspadyah Mawarni, Riski Eka Nariswari, Bintari Nindhyasari, Shintya Noviatri, Aldila Pratiwi, Herlina Priambudi, M. Zuliono Dwi Risky Ratna, Novia Kumala Dian Nur Safitri, Anna Setianingrum, Ani Untari, Handayu Widi Nugroho