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All Journal Indonesian Journal of Cancer Chemoprevention Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Ners Didaktik : Jurnal Ilmiah PGSD STKIP Subang Indonesian Journal of Medical Sciences and Public Health International Journal of Science and Society (IJSOC) International Journal of Health and Pharmaceutical (IJHP) Cerdika: Jurnal Ilmiah Indonesia Eduvest - Journal of Universal Studies
Harliansyah
Universitas YARSI
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) Harliansyah, .; Zurinah, W.N. Wan; Anum, M.Y. Yasmin; Azian, M Noor
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

The liver cells were cultured in the  presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while  apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities.  Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ±  17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500  μg/ml.  We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis.
Anti-Inflammatory Activity of The Extract of Guava Leaves (Psidium guajava L) in The Rat (Rattus norvegicus L) Weni, Linda; Harliansyah, .; Widayanti, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Using of natural sources that have anti-inflammatory activity for the prevention and treatment of degenerative diseases began to be further explored. An investigation on the anti-inflammatory activity of the aqueous extract of guava leaves (Psidium guajava L.) from Sawangan, Depok on white male rats of Sprague-Dawley strain had been carried out on the carrageenan-induced  paw  edema  method.    To  examine  the  effect  of  guava  extract  on subcutan at different doses of 125, 250 and 500 mg/kg of body weight (BW).  Indometacine at dose of 10 mg/kg BW was used as a positive control. Observations were made during five hours with an interval of one hour. These results demonstrate that the percentage of inflammation or edema (% E) optimal at the 4th hour and then decreased at the 5th hour, while the percentage of optimal inhibition occurred at the 5th hour. Guava extract at 125, 250 and 500 mg/kg BW reduced inhibitory percentage activities by 40.81, 55.45 and 43.61% (p<  0.05)  respectively.  In  conclusion,  this  study  suggests  that  guava  extract  has  anti-inflammatory properties by decreasing edema level.
The Role of Curcumin on Apoptosis through The RASSF1A and Bax Pathways in Breast Cancer Nunung Ainur Rahmah; Harliansyah Harliansyah; Fransiscus D. Suyatna; Mpu Kanoko; Primariadewi Rustamadji; Joedo Prihartono; Samuel Johny Haryono; Bethy Suryawati Hernowo
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp67-74

Abstract

Curcumin has been reported with an in vitro the cytotoxic effect on several human cancer cells. However, reports on the mode of action and detail mechanism of curcumin in breast cancer disease are limited. Hence, curcumin’s effect on the human breast cancer cell line MCF-7 and MDA-MB-468 was investigated. The MCF-7 and MDA-MB-468 breast cancer cells line were given curcumin in several doses. The anti-proliferation activity of curcumin was determined using the MTS cell viability test and caspase-3 activity was used to detect apoptosis using flowcytometry. The expression of Ras-association domain family 1 isoform A (RASSF1A) and Bax protein in cells was evaluated by ELISA analysis. Kruskal-Wallis followed by the Mann-Whitney test and the Spearman correlation tests were used to asses correlation among RASSF1A, Bax, and caspase-3. Cytotoxicity of curcumin on MCF-7 was lower than that of MDA-MB-468 (75.73 μg/mL and 380.79 μg/mL). The concentration of curcumin at 80 μg/mL induced apoptosis mainly through the intrinsic pathway by caspase-3 activation. Curcumin also showed an anti-proliferative activity as shown by the increase of RASSF1A and Bax protein. Curcumin mediates anti-proliferative and apoptotic effect through the activation of RASSF1A and Bax. Our research data adds information about the role of curcumin in epigenetic events through RASSF1A protein.Keywords: Bax, caspase-3, curcumin, MCF-7, MDA-MB-468, RASSF1A
α-Mangosteen as An Oxidative Inhibitor in Hepatocellular Carcinoma Harliansyah Harliansyah; Nunung Ainur Rahmah; Kuslestari Kuslestari
Indonesian Journal of Cancer Chemoprevention Vol 12, No 2 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss2pp106-113

Abstract

Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and the second leading cause of cancer mortality worldwide. Many strategies to discover molecular-based therapy are currently being implemented to overcome the resistance in HCC treatment. Cancer research is more targeted at molecular level of natural ingredients treatment as chemoprevention to reduce carcinogenesis risk. One of the natural compounds that serve as chemopreventive agent is mangosteen. α-Mangosteen, a xanthone commonly found in the fruit hull of Garcinia mangostana Linn, possess as an antioxidant. This study aims to determine the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and protein carbonyl (PC) as the biomarkers of oxidative stress on untreated HepG2 cells compared to α-mangosteen-treated HepG2 cells. The results indicated that α-mangosteen has a cytotoxic effect on HepG2 cells with IC50=242.58 μg/mL and reduced ROS level 23.15±4.29% at 200 μg/mL. The MDA level of HepG2 cells was not significantly higher than on WRL-68 by 7.6%, 17.93%, 28.8%, 35.32%, and 61.95% at 100, 200, 500, 800, and 1000 μg/mL respectively. α-Mangosteen at 100 and 200 μg/mL reduced protein carbonyl by 76.24 and 79.84% in HepG2 cells line while compared to normal liver cells line (WRL-68) significantly (P<0.05). In conclusion, α-mangosteen reduced levels of ROS, MDA and PC. Therefore, α-mangosteen is a potential anti-cancer agent through oxidative stress inhibition.Keyword: free radical, HepG2 cells, α-mangosteen, oxidative stress.
Anti-Inflammatory Activity of The Extract of Guava Leaves (Psidium guajava L) in The Rat (Rattus norvegicus L) Linda Weni; Harliansyah Harliansyah; Widayanti Widayanti
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp169-172

Abstract

Using of natural sources that have anti-inflammatory activity for the prevention and treatment of degenerative diseases began to be further explored. An investigation on the anti-inflammatory activity of the aqueous extract of guava leaves (Psidium guajava L.) from Sawangan, Depok on white male rats of Sprague-Dawley strain had been carried out on the carrageenan-induced paw edema method. To examine the effect of guava extract on subcutan at different doses of 125, 250 and 500 mg/kg of body weight (BW).  Indometacine at dose of 10 mg/kg BW was used as a positive control. Observations were made during five hours with an interval of one hour. These results demonstrate that the percentage of inflammation or edema (% E) optimal at the 4th hour and then decreased at the 5th hour, while the percentage of optimal inhibition occurred at the 5th hour. Guava extract at 125, 250 and 500 mg/kg BW reduced inhibitory percentage activities by 40.81, 55.45 and 43.61% (p< 0.05) respectively. In conclusion, this study suggests that guava extract has anti-inflammatory properties by decreasing edema level.Keywords: Anti-inflammatory, guava leaves, edema.
Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) Harliansyah Harliansyah; W.N. Wan Zurinah; M.Y. Yasmin Anum; M. Noor Azian
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev1iss2pp118-123

Abstract

The liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ± 17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500 mg/ml.  We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis.Keywords : antiproliferation, apoptosis, caspases, Zingiber officinale
Hubungan Durasi Paparan Gelombang Elektromagnetik Telepon Seluler Terhadap Kadar Melatonin Pada Tikus Putih (Rattus norvegicus) Galur Wistar Irrayanti Putri; Harliansyah Harliansyah
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 12, No 1 (2018): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.492 KB) | DOI: 10.33533/jpm.v12i1.337

Abstract

Background : Mobile Phone (MP) is a wireless communication tools that nowadays used by people with many features that can ease the user. Cellphone is releasing electromagnetic radiation that can causes side effect for the user itself, one example of the side effect is disturbing the melatonin hormone synthesis that can cause the abnormal sirkadian body rythm, so the sleep patterns are disrupted, causing insomnia, islam is teaching us to keep our health, for example is to keep the rythm of our bedtime regularly. Objective : Knowing the relation between exposure duration of mobile phone electromagnetic radiation to melatonin level of Wistar albino rats observed by medicine and islam perspectives. Methods : This study was experimental study, total sample was determined by frederer theory and analyzed using IBM SPSS Statistic 21 for Windows. Results : The results showed that melatonin levels in the positive control group (dark control group) 13,705 ± 0,500 were compared with the group which exposed for 3 hours 13,375 ± 0,654 with (p=0,299) and in the positive control group (dark control group) 13,705 ± 0,500 compared with the group which exposed for 6 hours 13,223 ± 0,529 with (p=0,116). Conclusions : There is no significant relation between exposure duration of mobile phone electromagnetic radiation to melatonin levels in Wistar albino rats.
RETRACTION NOTICE: Curcumin as an Anti-Proliferative Agent in Breast Cancer through RASSF1A, Bax, and Caspase-3 Protein Nunung Ainur Rahma; Harliansyah; Fransiscus D. Suyatna; Mpu Kanoko; Primariadewi Rustamadji; Joedo Prihartono; Arleni Bustami; Samuel Johny Haryono; Bethy Suryawati Hernowo
Indonesian Journal of Medical Sciences and Public Health Vol 2 No 1 (2021)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.023 KB) | DOI: 10.11594/ijmp.02.01.03

Abstract

Introduction: Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specific mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types. This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells. Method: Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using flow cytometry. Results: The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) significantly. Conclusions: Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.
The Comparison of the Parameters Antioxidant Activity of Ginger (Zingiber officinale Roscoe) and Commercial Antioxidants Harliansyah; Kuslestari
International Journal of Science and Society Vol 4 No 3 (2022): International Journal of Science and Society (IJSOC)
Publisher : GoAcademica Research & Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54783/ijsoc.v4i3.510

Abstract

The study was aimed at evaluating the antioxidant activity of Zingiber officinale Roscoe (ginger) extract compared to antioxidant compounds such as; Butyl Hydroxy toluene (BHT), Diethyl-dithiocarbamate (DDC) and Ascorbic Acid (Vit. C). The antioxidant activity (DPPH), total phenolic content (TPC) and reducing power (RP) were studied. DPPH inhibitory values demonstrated through IC50 from ginger extract were significantly lower than those of other antioxidants at the same concentrations: ginger (122.2 ± 16.78%), Vit. C (158.21 ± 4.14%), BHT (195.84 ± 5.94%) and DDC (240.39 ± 43.75%) respectively. This proves that ginger extract is more active inhibiting DPPH free radicals. At a concentration of 100 μg / ml, the TPC level indicates the order: Vit. C > DDC > BHT > ginger respectively. As for RP test at concentration 100 μg / ml also Vit. C > ginger > DDC > BHT respectively. The results obtained in present study indicate that, ginger extract is a potential source of natural antioxidant capable of inhibiting and breaking the free radical chain and it’s expected to suppress the development of degenerative cells diseases.
Study Healthy Ageing Lansia pada Masa Pandemi COVID-19 di Kelurahan Sukarame Bandar Lampung Ditinjau dari Kedokteran Shyffa Khairunnisa S. Raya; Harliansyah Harliansyah; Kuslestari Kuslestari
Cerdika: Jurnal Ilmiah Indonesia Vol. 2 No. 8 (2022): Cerdika: Jurnal Ilmiah Indonesia
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1263.653 KB) | DOI: 10.59141/cerdika.v2i8.437

Abstract

COVID-19 is a widespread global health problem with a rapid and easy way of transmission. With a high death rate, the government implements a lock down that indirectly reduces the space for people to move. This then relate to the health and productivity of the elderly in achieving healthy aging in the elderly. : This study aims to see if there is a healthy aging movement carried out by the elderly in Sukarame Village, Bandar Lampung during the COVID-19 pandemic. The method in this research is qualitative phenomenology. The study was conducted through interviews with respondents with criteria for healthy and active elderly in Sukarame Village, Bandar Lampung. Samples were determined by means of Purposive Sampling. The data were analyzed using three stages, namely data reduction, data presentation, and drawing conclusions using correlation test bivariate Spearman's Rho. In the study, it was found that 87% of respondents were still physically active. As many as 97% of respondents have a good and sufficient appetite. It was found that 75% of the respondents were non-smokers. Of the total respondents, there are 20% of respondents who are still working. All respondents are still involved in environmental and social activities. All the healthy aging carried out by the respondents are very strongly and strongly related to each other. The mood of the elderly has a significant impact on the satisfaction that the elderly feel. The healthy aging movement was found in respondents in Sukarame Village, Bandar Lampung.
Co-Authors . Widayanti Agfy Laila Fadhilah Ahmad Randy Ainuzzahra Angelica Vanini Winata Taufiq Arleni Bustami Bethy Suryawati Hernowo Bethy Suryawati Hernowo Dhina Lorenza Dian Widiyanti Etty Widayanti Fransiscus D. Suyatna Fransiscus D. Suyatna Irma Rosalina Irrayanti Putri Joedo Prihartono Joedo Prihartono Kuslestari Kuslestari Kuslestari Kuslestari Kuslestari Linda Weni Luh Ade Lela Arika Luh Ade Lela Arika M Noor Azian M. Noor Azian M.Y. Yasmin Anum M.Y. Yasmin Anum Mpu Kanoko Mpu Kanoko Nenden Lilis setiasih Nunung Ainur Rahma Nunung Ainur Rahmah Pendrianto Pendrianto Pratiwi Pujilestari Sudarmono Primariadewi Rustamadji Puput Putuh Anisa Raka Auriza Fathaya Ratu Salia Siskowati Regina Callista A. Tupamahu Rizkia Amartya Noor Salda Fransiska Samuel Johny Haryono Samuel Johny Haryono Shyffa Khairunnisa S. Raya W.N. Wan Zurinah W.N. Wan Zurinah Widayanti Widayanti