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All Journal Jurnal Filsafat Indonesian Journal of Agricultural Science Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences

Indraningsih .

Indonesian Veterinary Research Institute

Author-ID : 212151

Agriculture, Biological Sciences & Forestry Arts Humanities Education Languange, Linguistic, Communication & Media Social Sciences Veterinary

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Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant Widiastuti, R; Firmansyah, R; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 Ã-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-HÂ® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 Ã-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation

Neuropathology of organophosphate poisoning in dairy cattle Sani, Yulvian; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

The purpose of this study is to investigate pathological changes in brain tissues of Frisien Holstein dairy cattle affected by organophosphate (OP). The study was directed to anticipate spongiform encephalopathy. Samples consisted of animal feeds, sera and brain tissues were collected from Lembang, West Java.Â Animal feeds (fodders and commercial feed) were collected directly from the dairy farms around Lembang. Sera (31 samples) were from dairy cattle owned by the local farmers and brain tissues were from the local animal slaughter house. Pesticide residues were analysed following a standard procedure using gas chromatography (GC). There was an interaction between pesticide residues in animal feeds, residue level of pesticides in sera and brain tissues to cause encephalopathy in dairy cattle. Pesticide contamination in animal feeds was regarded as the source of encephalopathy in dairy cattle. The total average of OP residues (16.8 ppb) were lower than organochlorines/OC (18.7 ppb) in fodder, showing that pesticides were originated from the contaminated soils. On the other hand, the total average of OP residues in commercial feeds (12.0 ppb), sera (85.6 ppb) and brain tissues (22.7 ppb) were higher than OC (1.8; 16.7; and 5.1 ppb). The OP appears more frequently used for dairy farm activity as insecticides. Histopathological examination for brain tissues of dairy cattle showed that most cattle were diagnosed as encephalopathy with microscopic changes of vacuolation, neuronal necrosis, chromatolysis of neurons and nucleolysis of neurons. The encephalopathy was confirmed in rats intoxicated with chlorpyrifos methyl as severe brain damage with spongiform-like lesions. Key Words: Encephalopathy, Organophosphates, Cattle

Detection of deoxynivalenol and T-2 toxin in feeds by gas chromatography with Electron Captured Detector Widiastuti, R.; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Deoxynivalenol (DON) and T-2 toxin are trichothecene mycotoxins produced by Fusarium spp. may pose to threat animal health. This research was aimed to validate an analysis method for DON and T-2 toxin detection in feed using a gas chromatography with electron captured detector ECD (GC-ECD) and to study the contamination level of DON and T-2 toxin in feed. Samples were extracted with organic solvents and derivitized with DMAP and HFBA prior to the detection with GC. Both toxins can be detected simultaneously with satisfied validation with GC. Analysis for DON showed that 17 out of 24 corn samples were positive (0.450 to 1.126 mg/kg), none was positive for 20 rice husk samples and 1 out of 14 cattle feed samples was positive (0.043 mg/kg), whereas analysis for T-2 toxin showed that 2 out of 24 corn samples were positive (0.022 and 0.063 mg/kg), 14 out of 20 rice husk samples were positive (0.027 to 0.720 mg/kg) and 6 out of 14 cattle feed samples were positive (0.039 to 0.084 mg/kg). The study showed that DON and T-2 toxin can be detected using GC-ECD. Whereas, the analysis results from field samples showed that the feeds were still safe to be used for animal feed, however a routine surveillance is needed to minimize further potential hazards. Key words:Â Deoxynivalenol, T-2 toxin, Gas Chromatography, Feeds

Co-Authors R Firmansyah R Maryam R Widiastuti R. Widiastuti Raphaella Widiastuti Sjamsul Bahri T B Murdiati T.B Murdiarti Yulvian Sani

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