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Rinto .
Department Of Fisheries Product Technology, Faculty Of Agriculture, Universitas Sriwijaya
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Journal : agriTECH

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Isolasi dan Identifikasi Bakteri Asam Laktat Penghasil Inhibitor Enzim HMG-KoA Reduktase dari Bekasam Sebagai Agen Pereduksi Kolesterol Rinto Rinto; Ratih Dewanti; Sedarnawati Yasni; Maggy Thenawidjaja Suhartono
agriTECH Vol 35, No 3 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.273 KB) | DOI: 10.22146/agritech.9342

Abstract

The purpose of this research was to obtain statins producer bacteria as a HMG-CoA reductase (HMGR) enzyme inhibitor to reduced cholesterol biosynthesis. Stages of this research were the isolation of compactin and lovastatin resistant bacteria, statin production, analysis of culture extracts to inhibition of HMG-CoA reductase and identification of bacteria. The results showed that the 20 isolates of compactin and lovastatin resistant bacteria, there are 5 bacterial isolates produced statins. They were L3.3.4; C3.4.2; C3.3.5; C3.4.4 and L3.3.3; with the statins content were 9.491; 1.536; 0.065; 0.060; and 0.040 ppm. Selection of the 5 bacterial isolates resulted 2 bacteria which had inhibition ability to HMGR enzyme activity. They were Lactobacillus acidophilus and Lactobacillus delbruckii sp. delbruckii with inhibitory ability were 66.67% and 58.33%, respectively.ABSTRAKPenelitian ini bertujuan memperoleh bakteri penghasil statin sebagai inhibitor enzim HMG-KoA reduktase (HMGR), penghambat sintesis kolesterol. Tahapan penelitian yang dilakukan adalah isolasi bakteri yang resisten terhadap compactin dan lovastatin, produksi statin, uji penghambatan ekstrak dari kultur bakteri terhadap HMG-KoA reduktase dan identifikasi bakteri. Hasil penelitian menunjukan bahwa dari 20 isolat bakteri yang resisten terhadap compactin maupun lovastatin, terdapat 5 isolat bakteri yang potensial menghasilkan statin, yaitu isolat L3.3.4; C3.4.2; C3.3.5; C3.4.4 dan L3.3.3; dengan kandungan statin berturut-turut adalah 9.491; 1,536; 0,065, 0,060, dan 0,040 ppm. Seleksi terhadap 5 isolat menghasilkan 2 bakteri yang mempunyai kemampuan penghambatan terhadap aktivitas enzim ¸Â•ÝWtu Lactobacillus acidophilus dan Lactobacillus delbruckii sp. delbruckii dengan kemampuan penghambatan  berturut-turut adalah 66,67% dan 58,33%.
Analisis Bakteri Asam Laktat dan Senyawa Bioaktif selama Fermentasi Bekasam Ikan Nila (Oreochromis niloicus) Rinto Rinto; Herpandi Herpandi; Indah Widiastuti; Sabri Sudirman; Mega Purnama Sari
agriTECH Vol 42, No 4 (2022)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.70500

Abstract

Penelitian ini bertujuan untuk mengetahui perubahan komponen bioaktif dan bakteri asam laktat selama fermentasi bekasam serta untuk mengetahui media dan waktu terbaik dalam pembentukan komponen bioaktif selama fermentasi bekasam. Penelitian ini menggunakan tiga macam metode wadah penyimpanan fermentasi yaitu wadah toples, plastik vakum dan plastik non vakum, masing-masing sampel difermentasi selama 7, 11, dan 15 hari. Hasil data dianalisis dengan cara mendeskripsikan setiap parameter. Parameter yang diamati yaitu total Bakteri Asam Laktat, asam amino, asam lemak bebas, asam lemak dan lovastatin. Hasil penelitian menunjukkan bahwa perbedaan waktu fermentasi dengan penggunaan kemasan yang berbeda dapat menghasilkan perbedaan jumlah total Bakteri Asam Laktat (6,49-6,72 log cfu/ml), perbedaan kandungan komponen bioaktif berupa komposisi asam amino, kandungan asam lemak bebas (0,797-3,386%), komposisi asam lemak dan kandungan lovastatin (53,48-74,99 ppm).
Co-Authors Ace Baehaki Agus Supriadi Anjas Setiawan Dwi Inda Sari Dwi Indasari Eklin Meinatsya Putri Elmeizi Arafah Eva Lidya Febrimarani Malinda Ferdinand Hukama Taqwa Finanda Rahil Balqis Gama Dian Nugroho Hafif Subarka Herpandi . I Made Susila Utama Indah S Wahyuni Indah Widiastuti Indah Widiastuti Indah Widiastuti Indah Widiastuti Maggy Thenawidjaja Suhartono Mega Purnama Sari Mochamad Syaifudin, Mochamad Nanda Anggiani Putri Nurachma Pujiastuti Puspa Ayu Pitayati Puspa Ayu Pitayati Putri Ayu Anisa Ratih Dewanti -Hariyadi Ridhowati, Sherly Sabri Sudirman Sabri Sudirman SEDARNAWATI YASNI Shanti Dwita Lestari Shanti Dwita Lestari Shanti Lestari Siti Balqis Huriyah Siti Hanggita Rachmawati J Susi Lestari Susi Lestari Susi Lestari Tengku Zia Ulqodry Wulandari Wulandari Yosi Arianti Yunindyawati Yunindyawati