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Journal : METANA

KINETIKA FERMENTASI LIMBAH KULIT NANAS DAN PRODUKTIVITAS ETANOL Alice Pramashinta; Abdullah Abdullah
METANA Vol 10, No 01 (2014): Juli 2014
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1373.288 KB) | DOI: 10.14710/metana.v10i01.9772

Abstract

Abstract In this research studied the effect of initial total sugar concentration of pineapple peel waste in cell concentration, total sugar consumption and ethanol production profile. Pineapple peel fermentation is done by batch system for 60 hours with various intial total sugar 30, 50 and 70 g/L. Batch fermentation profiles showed that the optimum total sugar concentration was 70 g/L which resulted highest ethanol productivity 0.617 g/L/h when compared with total sugar concentration 30 g/L and 50 g/L. Kinetic parameters of Saccharomyces cerevisiae in batch fermentation using pineapple peel waste as substrate were also studied. Growth kinetic parameter was determined by Monod equation. Growth rate specific maximum 0.032 jam-1 and Monod constant Ks 17.18 g/L.   Keywords: bioethanol, pineapple, batch, kinetic, Saccharomyces cerevisiae Abstrak Pada penelitian ini akan dipelajari pengaruh konsentrasi gula total awal dari limbah kulit nanas pada profil pembentukan sel, konsumsi gula total dan produksi etanol. Fermentasi limbah kulit nanas dilakukan dengan sistem batch selama 60 jam dengan variasi gula total awal sebesar 30, 50 dan 70 g/L. Profil fermentasi batch menunjukkan bahwa konsentrasi gula total optimum adalah 70 g/L yang menghasilkan produktivitas etanol tertinggi 0,617 g/L/jam bila dibandingkan dengan konsentrasi gula total 30 g/L dan 50 g/L. Parameter kinetika dari Saccharomyces cerevisiae dalam fermentasi batch menggunakan limbah kulit nanas sebagai substrat juga dipelajari. Parameter kinetika pertumbuhan ditentukan dengan persamaan Monod. Kecepatan pertumbuhan spesifik maksimum 0,032 jam-1 dan konstanta Monod Ks 17,18 g/L.   Kata kunci: bioetanol, nanas, batch, kinetika, Saccharomyces cereviseae
PENGARUH FREKUENSI DAN WAKTU PENCUCIAN BERBANTU ULTRASONIK MENGGUNAKAN ISOPROPANOL TERHADAP KADAR GLUKOMANAN DAN VISKOSITAS TEPUNG PORANG (Amorphophallus oncophyllus) Lucia Hermawati Rahayu; Dyah Hesti Wardhani; Abdullah Abdullah
METANA Vol 9, No 01 (2013): Juli 2013
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.207 KB) | DOI: 10.14710/metana.v9i01.7208

Abstract

Abstract Konjac (Amorphophallus oncophillus) contains a  high economic value of soluble fiber glucomannan. Most of Indonesia konjac flour is exported in  low  (glucomannan content <65) hence  sell in cheap price. Quality of glucomannan could be improved by removing impurities such as starch and cellulose.  Application of              ultrasonic-assisted extraction (leaching) of non glucomannan compound is an effective  method to purify the konjac flour. The purpose of this study was to study the effect of  ultrasonic waves frequency and leaching time of   ultrasonic-assisted extraction method using aqueous isopropanol on glucomannan purification. The leaching conducted two frequencies  (20 and 40 kHz) and various extraction time (5, 10, 15, 20, 25 minutes). The results showed that the best condition  of glucomannan purification was achieved at  20 kHz for 10 minutes. In this conditon, glucomannan content , viscosity and yield were 76.1%,  12,800 cPs, and 96.1%, respectively. Keywords: konjac (Amorphophallus oncophillus), glucomannan, ultrasonic-assisted extraction, isopropanol
KINETIC STUDY OF THE UTILISATION OF DIFFERENT SUBSTRATES TO LACTIC ACID USING Lactobacillus delbrueckii Abdullah Abdullah; Hanapi bin Mat; Widayat Widayat
METANA Vol 6, No 01 (2009): April 2009
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5667.593 KB) | DOI: 10.14710/metana.v6i01.1830

Abstract

Abstract   Lactic acid fermentation includes several reactions in association with the microorganism growth.  A kinetic study was performed of the utilisation of multiple substrates to lactic acid using lactobacillus delbrueckii. Batch fermentation was performed to study effect of different substrates such as glucose, fructose and sucrose. The objective of this research is to study kinetics grow microbial. A anaerobic fermentation were studied in 3 litres stirred fermentor ( Biostat B Model) with working volume of 1 liter, temperature : 40 oC, pH = 6.0, inoculums size : 5% , sugar concentration =  20 g/1. During the first hours of fermentation, glucose and fructose accumulated in the medium and the rate of  hydrolysis of sucrose to glucose and fructose was faster than conversion of these substrate. The maximum concentration of glucose and fructose was 5.82 and 5. 14 g/l respectively. The sucrose, glucose, and fructose consumption completely utilized at 56, 68, and 104 hours, respectively. Kinetic parameter for maximum .specific growth rate in glucose, fructose and sucrose is 0.103, 0.0448, and 0.0398 (h-1), respectively. The saturation constant is 2.13, 2.67, and 3.32 g/1.   Key Words: Kinetic Study, Sugars, Lactic Acid, Maximum Specific Growth Rate and Saturation Constant