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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Pengendalian Hayati Berkala Ilmiah Pertanian Indonesian Journal of Biotechnology UNEJ e-Proceeding JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ALCHEMY Jurnal Penelitian Kimia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Proteksi Tanaman Tropis
HARDIAN SUSILO ADDY
Faculty Of Agriculture,University Of Jember, Jl. Kalimantan No. 37, Jember 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Indonesian Journal of Biotechnology

 1 
Distinguishing resistances of transgenic sugarcane generated from RNA interference and pathogen‐derived resistance approaches to combating sugarcane mosaic virus Weny Nailul Hidayati; Retnosari Apriasti; Hardian Susilo Addy; Bambang Sugiharto
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.65256

Abstract

Sugarcane mosaic virus (SCMV) is a causative agent that reduces growth and productivity in sugarcane. Pathogen‐derived resistance (PDR) and RNA interference (RNAi) are the most common approaches to generating resis‐ tance against plant viruses. Two types of transgenic sugarcane have been obtained by PDR and RNAi methods using a gene‐encoding coat protein (CP) of SCMV (SCMVCp). This research aimed to distinguish resistance of the two transgenic sugarcanes in combating SCMV through artificial viral inoculation. The experiment was conducted using transgenic sugar‐ cane lines validated by PCR analysis. Insertion of gene‐encoding CP in the transgenic lines was confirmed by amplification of 702 bp of DNA fragment of SCMVCp. After viral inoculation, mosaic symptoms appeared earlier, at 21 days post inoculation (dpi) in PDR transgenic lines, but was at 26 dpi in RNAi transgenic lines. Symptom observation showed that 77.8% and 50% of the inoculated plants developed mosaic symptoms in PDR and RNAi transgenic lines, respectively. RT‐PCR analysis revealed that the nuclear inclusion protein b (Nib) gene of SCMV was amplified in the symptomatic leaves in plants classified as susceptible lines. Immunoblot analysis confirmed presence of viral CP with a molecular size of 37 kDa in the susceptible lines. Collectively, these results indicated that the RNAi approach targeting the gene for CP effectively produces more resistance against the SCMV infection in transgenic sugarcane compared to the PDR approach.
Resistance gene expression in selected Indonesian pigmented rice varieties against infection by Xanthomonas oryzae pv. oryzae Nur Elia Nadhira; Ali Wafa; Wahyu Indra Duwi Fanata; Hardian Susilo Addy
Indonesian Journal of Biotechnology Vol 27, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.70445

Abstract

Rice (Oryza sativa L.) production is limited by bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo). For decades, researchers have attempted to control this disease by growing plants with blight‐resistant Xa genes. Genetic resources often vary between rice varieties, and there is little information about the genetic resources of the pigmented rice varieties widely grown in Indonesia and their resistance genes against Xoo. The purpose of this study was to determine the expression of Xa genes in pigmented rice such as Inpari 24 and Cempo Merah (red‐pigmented) along with Hitam Bantul (black‐pigmented) and white rice varieties IR64 and Ciherang, and to evaluate their resistance to BLB. All varieties carried the Xa4, Xa10 and xa13 genes but varied in the Xa1, Xa7 and Xa21 genes. The rice varieties expressed some of these genes only after inoculation with Xoo. Disease assessment categorised the three different pigmented rice varieties as resistant (Ciherang, Cempo Merah and Hitam Bantul), while IR64 (white) and Inpari 24 (red) were moderately resistant. There was no specific pattern of Xa genes possession, quality of expression or resistance level to X. oryzae pv. oryzae. Therefore, when breeding plants, the selection of parental variety must be considered in terms of the possession and expression of Xa genes such as Xa10 as a molecular marker for resistance.
Co-Authors .,, Rasmiyana Abdul Majid Abdul Majid Agus Wedi Pratama Agustina Wati, Fajar Dwi Ali Wafa Ankardiansyah Pandu Pradana Ariestya Ayu Meda Sallytha Ariestya Ayu Meda Sallytha Bambang Piluharto Bambang Sugiharto BUDI ARMAN Budi Kriswanto Budi Kriswanto, Budi Dwi Indarti Erlia Narulita Fajar Dwi Agustina Wati Fanata, Wahyu Indra Duwi Hadi, Zul Fauzi Nugroho Hardiyani, Wulan Arum Mohammad Khoirul Amrulloh N, Suhartiningsih Dwi Nur Elia Nadhira Nurul Rama Dhany Paniman Ashna MIHARDJO Paniman Ashna Mihardjo Prastowo , Sigit Rachmi Masnilah Restanto, Didik Pudji Retnosari Apriasti Slameto . Slameto Slameto Suhartiningsih Dwi N Suhartiningsih Dwi Nurcahyanti Tanti Haryati TRI CANDRA SETYOWATI TRI HANDOYO Tri Handoyo Wahyu Indra Duwi Fanata Wardhani, Widi Kusuma Weny Nailul Hidayati WIWIEK SRI WAHYUNI