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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Planta Tropika UNEJ e-Proceeding BIOVALENTIA: Biological Research Journal
Budi Kriswanto, Budi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
PRIMACY OF LIQUID MEDIUM TECHNIQUE ON PROTOCORM LIKE BODIES PROPAGATION OF Phalaenopsis sp ORCHIDS IN TISSUE CULTURE Kriswanto, Budi; Soeparjono, Sigit; Restanto, Didik Pudji
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1283.959 KB) | DOI: 10.24233/BIOV.6.1.2020.169

Abstract

Tissue culture have been used for plant propagation generally, and the medium has been important role in its growth. Vegetative propagation on Phalaenopsis sp orchids can be through the protocorm like bodies (PLB). Medium of affect on propagation of PLB was carried out on medium type, kind of basal medium and concentrations ratio of naphtaleneacetic acid (NAA) and benzylamino purine (BAP). The experiment used Completely Randomized Factorial Design with 3 replications and continued with the Duncan Multiple Range Test (DMRT) if there were significant differences. The results showed that the best callus formed in a combination of solid medium type and Murashige & Skoog (MS) basal medium was 100%. The most number of PLB produced from a combination of liquid medium types and a concentration ratio of NAA 1 mgL-1 and BAP 5 mgL-1, the most number of plantlet produced from a combination of MS basal medium and the concentrations ratio of NAA 0.1 mgL-1 and BAP 0.1 mgL-1, the number of PLB germination and PLB with leaves were influenced by each single factor.
The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B) Restanto, Didik Pudji; Aji, Seto Purnomo; Handayani, Etty; Ratnasari, Tri; Jadmiko, Mochammad Wildan; Prayoga, Mohammad Candra; Khozin, Mohammad Nur; Kriswanto, Budi
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18063

Abstract

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).
Nitrogen Fertilizer Reduction on Way Apo Buru and Inpari 33 Rice Varieties Slameto; Fariroh, Indri; Rusdiana, Riza Yuli; Kriswanto, Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.381 KB) | DOI: 10.24831/jai.v50i2.39299

Abstract

Pemupukan urea di petani umumnya dilakukan dengan dosis yang lebih tinggi dari standar pemerintah. Penggunaan pupuk urea berlebih dapat menurunkan produksi, kualitas tanaman, serta pencemaran lingkungan. Tujuan percobaan ini adalah melihat respon pertumbuhan, hasil dan kualitas padi sawah pada media N rendah. Rancangan percobaan menggunakan rancangan acak kelompok faktorial, dosis pupuk urea (0%, 40%, dan 100% 250 kg ha-1) dan varietas padi (Way Apo Buru dan Inpari 33). Parameter yang diamati adalah tinggi tanaman (cm), jumlah anakan, jumlah daun, chlorophyl fluorescence (unit), gabah isi (%), jumlah malai, kandungan protein terlarut (mg g-1), vitamin C (mg g-1), dan gula reduksi (mg g-1). Hasil penelitian menunjukkan bahwa reduksi pupuk urea 40% menghasilkan jumlah anakan yang sama tinggi dengan dosis standar (250 kg ha-1). Pemupukan urea dosis standar menghasilkan kandungan vitamin C, nilai chlorophyl fluorescence dan jumlah malai yang tertinggi. Inpari 33 menghasilkan karakter tinggi dan persentase gabah isi yang lebih baik sementara Way Apo Buru menghasilkan jumlah daun yang lebih tinggi. Reduksi 40% dosis urea pada Inpari 33 menghasilkan kandungan protein terlarut tertinggi. Inpari 33 menghasilkan gula reduksi terendah sehingga dapat digunakan sebagai pangan alternatif bagi penderita diabetes. Kata kunci: gula reduksi, kandungan klorofil, protein terlarut, vitamin C
Co-Authors Aji, Seto Purnomo Handayani, Etty HARDIAN SUSILO ADDY Khozin, Mohammad Nur Mochammad Wildan Jadmiko Prayoga, Mohammad Candra Ratnasari, Tri Restanto, Didik Pudji Rusdiana, Riza Yuli Sigit Soeparjono Slameto Slameto Slameto TRI HANDOYO