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All Journal Agrikultura Jurnal Ilmu Dasar Berkala Ilmiah Pertanian Jurnal Agro JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Pengabdian Magister Pendidikan IPA
Fanata, Wahyu Indra Duwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Mathematics

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Pengaruh Penambahan Inhibitor Etilen dan Senyawa Antioksidan terhadap Regenerasi Kalus Padi Mentik Wangi Susu Fanata, Wahyu Indra Duwi; Setiawan, Dedi Edo; Sholikhah, A’idah Mar’atus
Agrikultura Vol 33, No 2 (2022): Agustus, 2022
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v33i2.38064

Abstract

Kalus padi Mentik Wangi Susu memiliki tingkat regenerasi tunas yang rendah karena tingkat pencokelatan kalus yang tinggi dalam media regenerasi. Pencokelatan kalus dipicu oleh tingginya akumulasi etilen dan fenol selama kultur in vitro. Penelitian ini bertujuan untuk membandingkan efikasi antara inhibitor etilen dan antioksidan dalam mengurangi pencoklatan kalus serta meningkatkan regenerasi tunas pada kalus Mentik Wangi Susu. Perlakuan dilakukan dengan melakukan penambahan inhibitor etilen (putrecine atau perak nitrat) atau antioksidan (polyvinylpyrrolidone atau asam askorbat) dengan konsentrasi yang berbeda ke semua media kultur jaringan. Data yang diperoleh kemudian dianalisis dengan Analysis of Variance (ANOVA) dan Uji Beda Nyata Jujur (BNJ) dilakukan apabila antar perlakuan menunjukkan perbedaan yang nyata. Hasil penelitian menunjukkan tingkat pencoklatan kalus Mentik Wangi Susu hanya dapat dihambat secara nyata melalui penambahan polyvinylpyrrolidone ke dalam media regenerasi. Selain itu, perlakuan polyvinylpyrrolidone 0,3 g/l dan putresin 10 mM menghasilkan regenerasi tunas masing-masing sebesar 47% dan 41%, yang tertinggi di antara perlakuan lainnya. Oleh karena itu, polyvinylpyrrolidone dan putresin masing-masing merupakan antioksidan dan inhibitor etilen terbaik untuk meningkatkan regenerasi tunas kalus Mentik Wangi Susu.
Design and construction of single guide RNA for CRISPR/Cas9 system based on the xa13 resistance gene in some varieties of rice (Oryza sativa) Hardiyani, Wulan Arum; Wafa, Ali; Fanata, Wahyu Indra Duwi; Addy, Hardian Susilo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12347-55

Abstract

The xa13 gene is a recessive resistance gene against Xanthomonas oryzae pv. oryzae (Xoo) found in several rice varieties. Activation of this gene will trigger the formation of sucrose as a nutrient supply to Xoo for their growth in the plant. The disruption of this recessive gene expression in the plant can affect the negative impact of the gene, and recently can be created using clustered regularly interspaced short palindromic repeats (CRISPR) system using CRISPR-associated protein-9 (CRISPR/Cas9) technology that requires gRNA to recognize the targeted-sequence. This study aimed to design and construct the gRNA-targeting xa13 gene in rice using bioinformatics tools. CHOPCHOP was used for generated the gRNA candidates according to the target gene sequence. Two candidates of gRNA-targeted xa13 have been selected based on the analysis of bioinformatics data. Each candidate of gRNA consisted of 20 nucleotides (nt) of the target sequence upstream 3 nt of the protospacer adjacent motif (PAM) sequence (5’-NGG) targeting two exons in the xa13 gene. The gRNA1 will target exon 1 and the gRNA2 will target exon 2, with an efficiency of 52.51% and 44.63% respectively. Data showed that the GC content of all gRNA candidates ranged from 55–70% with no target-off location in the whole genome of rice. The transformation and confirmation test based on the physiological and genomic characteristics of transformants confirmed that the design has been successfully constructed.
The Determination of Aromatic Character of Several Local Rice Varieties using Phenotypic Analysis and Molecular DNA Fanata, Wahyu Indra Duwi; Husna, Syafira Fatihatul
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.12010

Abstract

The pandan scent in aromatic rice has been known as the result of 8 bp deletions and 3 Single Nucleotide Polymorphisms (SNPS) in BADH2 gene, which produce non-functional betaine aldehyde dehydrogenase (BADH) enzyme. Several DNA markers for aromatic character based on mutation in BADH2 gene have been developed. In our experiment, we analysed the presence of aromatic character in four local rice variety such as Merah Wangi, Pendok, Genjah Arum, and Mentik Wangi Susu using KOH method and DNA molecular method using three DNA markers to detect mutation that responsible for the development of aromatic character. Phenotype analysis using KOH method showed that Merah Wangi, Genjah Arum, and Mentik Wangi Susu produce pandan scents. PCR analysis using Bradbury and Badex7-5, and RM223 markers showed the presence of BADH2 mutation in Merah Wangi and Mentik Wangi Susu, whereas Pendok and Genjah Arum did not show BADH2 mutation using those used three markers. Our results indicate that among four investigated local rice, only Merah Wangi and Mentik Wangi Susu are categorized as aromatic rice whereas Pendok and Genjah Arum are non-aromatic.Keywords: genetic analysis, aromatic rice, specific markers, genetic mutation.
Effect of Potassium Fertilization on Growth and Head Rice Yield of Rice (Oryza sativa L.) Merah Wangi Variety Fanata, Wahyu Indra Duwi; Usmadi, Usmadi; Ramadhan, Galang Rizki
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.652 KB) | DOI: 10.19184/jid.v21i1.9144

Abstract

The fertilization management is one strategy to optimize the quantity and quality of rice production. Our study was aimedto investigate the effect of potassium fertilization several agronomical parameters andthe rate of head rice after milling process. The study was conducted from March 2018 to July 2018 in the Agrotechnopark experimental field University of Jember in District of Sukorambi, Jember. Randomized Block Design (RBD) was used with the frequency of potassium chlorice (KCl) fertilization asthe single factor. The conducted potassium treatments were as follows: without KCl fertilization (control), one-time KCl fertilization, two times KCl fertilization, three times KCl fertilization and each fertilization level was conducted by sixrepetitions. The agronomic parameters such as plant height, tiller number, flowering time, rate of productive tillers, grain number per panicle, weight of 1000 grains, and percentage of filled grain were analyzed and percentage of head rice rate was determined as quality parameter. Our results showed that different treatment of potassium did not significantly affect the plant height, tillernumber, flowering time, rate of productive tillers, total grains per panicle, and weight of 1000 grains. On the other hands, the percentage of filled grainand the rate of head rice after milling were significantly increased by potassium fertilization. Compare to the control treatment, three times potassium fertilization resulted 9.24% and 13.83% increase of filled grain and head rice after two times milling, respectively. Our result suggest that potassium fertilization is important for grainquality of Merah Wangi rice variety. Keywords: Merah Wangi, potassium fertilization, growth, physical quality.
Pengaruh Penyemprotan Monokalium Fosfat dan Pemangkasan Pucuk terhadap Pertumbuhan dan Hasil Produksi Tanaman Cabai Rawit (Capsicum frustescens L.) pada Hidroponik Sistem Dutch Bucket Setyadi, Farhad Dwi; Fanata, Wahyu Indra Duwi
Berkala Ilmiah Pertanian Vol 7 No 1 (2024): Februari
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v7i1.44847

Abstract

Cayenne pepper plant (Capsicum frustences L.) is a plant that is synonymous with its spicy taste, so consumption needs are increasing. However, production of cayenne pepper plants has decreased due to a decrease in land conversion and climate influences. Application of hydroponic dutch bucket system and monopotassium phosphate (MKP) spraying as well as shoot pruning techniques at certain times to increase production. This research aims to determine the effect of MKP spraying and shoot pruning on the growth and production of cayenne pepper plants using the dutch bucket hydroponic system. The experimental design used two factorsthree test. The first factor is variation in concentration of MKP administration, with 3 levels. The second factor is the pruning time with 3 levels so that there are 27 experimental units. The data is then analyzed and evaluated using analysis of Variance (ANOVA). Each observed variable is analyzed separately and significant differences between average values are based on tests Duncan Multiple Range Test (DMRT) with a confidence level of 95%. The results show for the stem diameter variable, the monopotassium phosphate spraying treatment gave very significantly different results and shoot pruning gave significantly different results.The combination of monopotassium phosphate spraying treatment with a dose of 4 g/liter and a shoot pruning time of 21 days after planting (M3N2) gave the highest results for the variables of stem diameter, number of flowers to fruit, number of fruit, and fruit weight.
Pengaruh Penambahan Monokalium Fosfat Dan Media Tanam Terhadap Produktivitas Tanaman Mentimun (Cucumis sativus L.) Pada Hidroponik Sistem Tetes Hartono, Budi; Fanata, Wahyu Indra Duwi
Berkala Ilmiah Pertanian Vol. 7 No. 3 (2024): Volume 7 Nomor 3
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v7i3.42173

Abstract

Mentimun merupakan komoditas hortikultura yang sering dikonsumsi dan dibudidayakan di Indonesia. Tingkat produksi mentimun di Indonesia mengalami peningkatan dari tahun 2018 hingga tahun 2021, namun pada tahun 2022 produksi mentimun mengalami penurunan. Tahun 2018 produksi ketimun sebanyak 433.931 ton, tahun 2019 sebanyak 435.975 ton, tahun 2020 sebanyak 441.286 ton, tahun 2021 sebanyak 471.941 ton. Pada tahun 2022 produksi akan turun menjadi 444.057 ton (BPS, 2022). Peningkatan produksi mentimun juga dapat menggunakan urban farming yang salah satunya menggunakan sistem hidroponik. Sistem hidroponik sangat membutuhkan media tanam sebagai tempat tumbuhnya sistem perakaran tanaman agar pertumbuhan tanaman lebih maksimal dengan menggunakan media tanam yang tepat. Penelitian ini menggunakan media tanam berupa cocopeat, arang sekam, dan arang kayu. Selain menggunakan media tanam, penelitian ini menggunakan konsentrasi MKP 270 ppm, 420 ppm, dan 570 ppm. Penambahan konsentrasi monopotassium fosfat dengan semua perlakuan tidak berpengaruh terhadap hasil produksi mentimun. Media tanam berpengaruh nyata terhadap semua variabel penelitian kecuali diameter buah. Cocopeat berpengaruh nyata terhadap produksi mentimun dibandingkan dengan media arang sekam dan media arang kayu. Interaksi antara penambahan konsentrasi monopotassium fosfat dan media tanam tidak berpengaruh nyata terhadap produksi mentimun. Kata Kunci: Mentimun, Hidroponik, Media tanam, MKP
Analisis Korelasi Karakter Agronomi Kuantitatif Terhadap Penentu Hasil Biji Kacang Kratok (Phaseolus Lunatus) Subekti, Andri Oktavian; Adie, Moch Muchlish; Fanata, Wahyu Indra Duwi
Berkala Ilmiah Pertanian Vol 7 No 2 (2024): Mei 2024
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v7i2.41196

Abstract

Tujuan penelitian ini untuk mengetahui karakter kuantitatif yang memiliki korelasi yang erat dengan karakter hasil yaitu berat biji per tanaman dan dapat dijadikan karakter seleksi efektif penentu hasil biji kacang kratok (Phaseolus lunatus). Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok (RAK). Aksesi yang digunakan merupakan aksesi benih dari kacang kratok (Phaseolus lunatus) sebanyak 20 aksesi. Penelitian ini dilakukan dengan 3 kali ulangan sehingga total tanaman yaitu 60 tanaman. Pertama data hasil karakter kuantitatif dianalisis dengan menggunakan ANOVA dan apabila terdapat karakter menunjukkan hasil berbeda nyata dilanjutkan dengan uji lanjut dengan uji Beda Nyata Jujur (BNJ) atau Tukey dengan taraf 5 %. Kedua data karakter kuantitatif dianalisis dengan analisis korelasi dan dilanjutkan dengan menggunakan analisis lintas untuk mencari karakter kuantitatif yang berpengaruh langsung dan tidak langsung terhadap hasil yaitu karakter berat biji per tanaman. Hasil ANOVA didapatkan karakter yang memperlihatkan hasil berbeda nyata yaitu hari berbunga dan berbeda sangat nyata yaitu karakter jumlah cabang dan panjang polong. Hasil analisis korelasi didapatkan karakter yang memiliki korelasi dengan karakter berat biji per tanaman yaitu karakter jumlah polong sebesar 0,655 (kuat), karakter berat polong sebesar 0,898 (sangat kuat) dan karakter berat 100 biji sebesar 0,578 (cukup kuat). Kemudian dilakukan analisis lintas terhadap ketiga karakter tersebut. Berdasarkan hasil analisis lintas dari ketiga karakter tersebut, karakter yang menunjukan pengaruh paling besar dan signifikan yaitu karakter berat polong dengan nilai total pengaruh yang signifikan sebesar 0,871 atau 87,1%. Oleh karena itu dapat disimpulkan bahwa karakter berat polong dapat dijadikan karakter seleksi yang efektif untuk menentukan hasil biji tanaman kacang kratok (Phaseolus lunatus).
PENGARUH VARIASI MEDIA TANAM DAN DOSIS NUTRISI AB MIX TERHADAP PERTUMBUHAN DAN HASIL TANAMAN TOMAT (SOLANUM LYCOPERSICUM L.) DENGAN HIDROPONIK SISTEM DUTCH BUCKET Saydi, Royhan; Fanata, Wahyu Indra Duwi; Ristiyana, Suci; Saputra, Tri Wahyu
Jurnal Agrotek Tropika Vol 10, No 4 (2022): JURNAL AGROTEK TROPIKA VOL 10, NOVEMBER 2022
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i4.6061

Abstract

Tomat (Solanum lycopersicum L.) terus mengalami peningkatan produksi seiring tingginya permintaan. Produksi tinggi yang berkualitas menuntut adanya pengembangan metode dan salah satu solusinya yaitu menggunakan Dutch Bucket Hydroponic System. Metode ini membutuhkan sebuah penelitian dengan tujuan mengetahui pengaruh perlakuan yang diberikan dan perlakuan terbaik untuk rekomendasi bagi petani maupun praktisi. Rancangan penelitian berbasis metode Rancangan Acak Lengkap dengan dua perlakuan yaitu variasi media tanam yaitu cocopeat (A1), hidroton (A2), dan rockwool (A3) sedangkan variasi konsentrasi nutrisi AB mix yaitu 1000 ppm (A1), 2000 ppm (A2), dan 3000 ppm (A3). Penelitian dilakukan dengan 9 kombinasi perlakuan dan 3 ulangan dengan mengamati diameter batang, tinggi tanaman, jumlah bunga, jumlah buah, berat segar tanaman, berat basah akar, dan berat buah pertanaman. Lokasi penelitiam di Agrotechnopark Universitas Jember pada bulan Januari-April 2022 dengan metode ANOVA dan DMRT. Perlakuan jenis media tanam, konsentrasi nutrisi AB mix, dan interaksi keduanya berpengaruh signifikan terhadap parameter penelitian. Hasil terbaik pada berbagai perlakuan terdapat pada media tanam cocopeat dan konsentrasi nutrisi 2000 ppm yang dapat menjadi rekomendasi dalam budidaya tanaman tomat dengan sistem hidroponik dutch bucket.
Pengaruh Konsentrasi Pupuk STMJ Plus (Susu Telur Molase Jamur Trichoderma sp. dan Limbah Buah-Buahan) terhadap Pertumbuhan dan Hasil Dua Varietas Selada (Lactuca sativa L.): Pengaruh Konsentrasi Pupuk STMJ Plus (Susu Telur Molase Jamur Trichoderma sp. dan Limbah Buah-Buahan) terhadap Pertumbuhan dan Hasil Dua Varietas Selada (Lactuca sativa L.) Ummah, Anisatul; Arum, Ayu Puspita; Setiyono, Setiyono; Fanata, Wahyu Indra Duwi; Tanzil, Ahmad Ilham
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 8 No 2 (2024): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v8i2.465

Abstract

Increasing lettuce production will be done by improving cultivation inputs, such as increased nutritional needs. An alternative organic fertilizer that can be used is made from spoiled milk, eggs, Trichoderma sp. molasses, and fruit waste, or STMJ Plus for short. STMJ Plus Fertilizer acts as an organic nutrient to encourage plant growth. This study aimed to determine the effect of the interaction between STMJ Plus fertilizer concentrations and lettuce varieties on the growth and yield of lettuce plants. The experiment was carried out in a factorial manner using a completely randomized design (CRD) consisting of 2 factors with 4 replications. The first factor is the concentration of STMJ Plus fertilizer consisting of 4 levels: P0: without fertilizer; P1: 5 ml L-1; P2: 10 ml L-1; and P3: 15 ml L-1. The second factor was the variety, which consisted of 2 levels: V1: Grand Rapids and V2: Red Rapid. The results of this study that the interaction of STMJ Plus fertilizer concentrations and lettuce varieties showed highly significant differences in plant height and plant fresh weight variables. The combination that gave the best effect was the concentration of 15 ml L-1 on the Red Rapid (P3V2).
The Effect of Nutritioning Interval on Automatic Drip Hydroponic System on Growth and Production of Three Varieties of Lettuce (Lactuca sativa L.) Ristiyana, Suci; Fanata, Wahyu Indra Duwi; Saputra, Tri Wahyu; Purnamasari, Ika; Dewanti, Parawita; Taufik, Rahadian Falqi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.146-154

Abstract

Lettuce (Lactuca sativa L.) is a leaf vegetable that has a high level of consumer demand and commercial value and can be cultivated using a combination of hydroponic substrate and drip irrigation methods. This research aims to determine the effect of the time interval for providing nutrients on the growth and harvest results of three lettuce plant varieties. Plants were cultivated on cocopeat and husk charcoal media (ratio 1:3) and arranged using a Randomized Block Design (RAK) consisting of two treatments. The first treatment is the nutritional interval which consists of an interval of 2 hours with a discharge of 17 ml (I1), 3 hours with a discharge of 25 ml (I2), and 4 hours using a discharge of 34 ml (I3). The second treatment is the plant variety which consists of the Grand Rapids variety (V1), the Green Coral variety (V2), and the Red Coral variety (V3). The data is analyzed using analysis of variance and if the results obtained are significantly different then a DMRT test will be carried out. The research results showed that the Green Coral lettuce variety was better than the Grand Rapids and Red Coral varieties. This is shown by the results with the highest and best values for the observation variables of number of leaves, plant fresh weight, and chlorophyll content. The nutritional interval which consists of an interval of 2, 4, and 6 hours gave results that were inversely proportional to the variety treatment, that is, they were not significantly different in all observed variables. Keywords: lettuce plants, plant varieties, nutritional interval, substrate hydroponics
Co-Authors . Usmadi Adie, Moch Muchlish Ahmad Ilham Tanzil Ali Wafa Ariyono, Alief Rizky Arum, Ayu Puspita Asshidiqie, Wahyu Pratama Bambang Sugiharto Budi Hartono HARDIAN SUSILO ADDY Hardiyani, Wulan Arum Hari Purnomo Husna, Syafira Fatihatul Ika Purnamasari Khamidah, Khusna Maghfirah, Intan Hadiatun Muhammad, Adibillah Muhtadi, Much. Misbah Neliana, Intan Ria Nikmah, Nasilatun Nisa, Aminatun Novitasari , Adin Parawita Dewanti Raharja, Ajung Gilang Putra Ramadhan, Galang Rizki Ramadhani, Rifki Fahrisal Ratnasari, Tri Ristiyana, Suci Saydi, Royhan Setiawan, Dedi Edo Setiyono Setiyono Setyadi, Farhad Dwi Sholikhah, A’idah Mar’atus Subekti, Andri Oktavian Syafina Pusparani Taufik, Rahadian Falqi TRI HANDOYO Tri Wahyu Saputra Ummah, Anisatul Ummi Sholikhah Utami, Sri Rejeki Wahyu Nurkholis Hadi Syahputra Yusuf Rachmandika