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All Journal HAYATI Journal of Biosciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Sosiohumaniora Jurnal Ilmiah Aplikasi Isotop Radiasi Microbiology Indonesia AL KAUNIYAH Jurnal Biologi dan Pembelajarannya (JBP) Jurnal Sosioteknologi Journal of Mathematical and Fundamental Sciences Jurnal Riset Kimia JURNAL BIOLOGI INDONESIA Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Agribisnis Terpadu Journal of Management - Small and Medium Enterprises (SME's) Makara Journal of Science
PINGKAN ADITIAWATI
Microbial Biotechnology Research Group, School of Life Sciences and Technology Institut Teknologi Bandung, Jl. Ganesha 10 Bandung, Jawa Barat 40132, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary

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Journal : Makara Journal of Science

 1 
Isolation of Asphaltene-Degrading Bacteria from Sludge Oil Aditiawati, Pingkan; Kamarisima,
Makara Journal of Science Vol. 19, No. 1
Publisher : UI Scholars Hub

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Abstract

Sludge oil contains 30%–50% hydrocarbon fractions that comprise saturated fractions, aromatics, resins, and asphaltene. Asphaltene fraction is the most persistent fraction. In this research, the indigenous bacteria that can degrade asphaltene fractions from a sludge oil sample from Balikpapan that was isolated using BHMS medium (Bushnell-Hass Mineral Salt) with 0.01% (w/v) yeast extract, 2% (w/v) asphaltene extract, and 2% (w/v) sludge oil. The ability of the four isolates to degrade asphaltene fractions was conducted by the biodegradation asphaltene fractions test using liquid cultures in a BHMS medium with 0.01% (w/v) yeast extract and 2% (w/v) asphaltene extract as a carbon source. The parameters measured during the process of biodegradation of asphaltene fractions include the quantification of Total Petroleum Hydrocarbon (g), log total number of bacteria (CFU/ml), and pH. There are four bacteria (isolates 1, 2, 3, and 4) that have been characterized to degrade asphaltic fraction and have been identified as Bacillus sp. Lysinibacillus fusiformes, Acinetobacter sp., and Mycobacterium sp., respectively. The results showed that the highest ability to degrade asphaltene fractions is that of Bacillus sp. (isolate 1) and Lysinibacillus fusiformes (Isolate 2), with biodegradation percentages of asphaltene fractions being 50% and 55%, respectively, and growth rate at the exponential phase is 7.17x107 CFU/mL.days and 4.21x107 CFU/mL.days, respectively.
Sequential Isolation of Saturated, Aromatic, Resinic, and Asphaltic Fractions Degrading Bacteria from Oil Contaminated Soil in South Sumatera Munawar, Munawar; Aditiawati, Pingkan; Astuti, Dea Indriani
Makara Journal of Science Vol. 16, No. 1
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Sequential isolation has been conducted to obtain isolates of saturated, aromatic, resin, and asphaltene fractions degrading bacteria from oil contaminated sites. Five soil samples were collected from South Sumatera. These bacterial isolates were obtained using soil extract medium enriched with oil recovery or remaining-oil recovery degradated (ROD) as sole carbon and energy sources according to the isolation stage as the isolation medium. ROD at the end of every isolation stage analyzed oil fractions by use of the SARA analysis method. Six isolates of bacteria have been selected, one isolate was fraction saturates degrading bacteria that are Mycobacterium sp. T1H2D4-7 at degradation rate 0.0199 mgs/h with density 8.4x106 cfu/g from stage I. The isolate T2H1D2-4, identified as Pseudomonas sp. was fraction aromatics degrading bacteria at accelerate 0.0141 mgs/h with density 5.1x106 cfu/g are obtained at stage II. Two isolates namely Micrococcus sp. T3H2D4-2 and Pseudomonas sp. T1H1D5-5 were fraction resins degrading bacteria by accelerate 0.0088 mgs/h at density 5.6x106 cfu/g and 0.0089 mgs/h at density 5.7x106 cfu/g are obtained at stage III. Isolation of stage IV has been obtained two isolates Pseudomonas sp. T4H1D3-1and Pseudomonas sp. T4H3D5-4 were fraction asphaltenes degrading bacteria by accelerate 0.0057 mgs/h at density 5.6x106 cfu/g and accelerate 0.0058 mgs/h at density 5.7x106 cfu/g.
Co-Authors . AKHMALOKA Agus Pujobroto Agustina L.N. Aminin Akhmad Zakaria Anthoni Agustien Crhisterra Ellen Kusumaningrum Dadang Sumardi Dadang Sumardi Dadang Sumardi Dea Indriani Astuti Dea Indriani Astuti Dea Indriani Astuti Dwiwahju Sasongko Dwiwahju Sasongko Fatimah, Anisah Nur Felicia Irene Saputra FIDA MADAYANTI Gede Suantika Gede Suantika Harry Rahmadi Heni Yohandini Herawati, Elysabet Indra Rudiansyah Indriani, Dea Indriani, Dea Irawan Sugoro Irawan Sugoro Irawan Sugoro Kamarisima Kamarisima Kamarisima, Kamarisima, Kamarisima Kusnadi Kusumawardhani, Meirina Kartika Liliasari Magdalena Lenny Situmorang Malendra Rusni Markhamah, Nadya Julia Megga Ratnasari Pikoli Mia Rosmiati Mia Rosmiati Mia Rosmiati Munawar . Munawar Munawar Novi Mulyawati, Novi Nurhajati, Jetty Osman R. Turendro PRIMA ENDANG SUSILOWATI Putri, Sastia Prama Rifki R. Arief Rosmiati, Mia Sandra Hermanto Sastia Prama Putri SONY SUHANDONO Srikandace, Yoice Syani, Ira Rhabbiyatun Togar M Simatupang Trisna Amelia Udin, Linar Z. Ulya Alviredieta Wahhaab, Aisha