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Morphological profile and estimation of genetic distance of Simmental crossbred Agung, Paskah Partogi; Ridwan, M; ., Handrie; ., Indriawati; Saputra, F; ., Supraptono; ., Erinaldi
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.655 KB) | DOI: 10.14334/jitv.v19i2.1039

Abstract

Research was done to study the profile of morphological characteristics of Simmental cross cattle in West Sumatera on several age categories and estimating the genetic distance compared to Simmental pure breed based on morphological parameters. Several morphological parameters of the 82 of Simmental cross cattle in West Sumatera and the 23 of Simmental pure breed has been measured. To analyze morphological data and the genetic distance a discriminant analysis was done. Based on statistical tests (t-test), body length, body height, hip height, hip width, chest circumference, chest width, chest depth, head length, and head height had significant difference (P<0.05) between 24-60 months Simmental pure breed and Simmental cross cattle bulls. Based on the similarity phenotypic analysis, it is known that all subpopulations of cattle in this research had a relatively high value (73.68-78.57%). Chest depth was the highest different morphometric factor (0.910). Morphological distribution map indicated that the Simmental cross cattle (subpopulations Agam and Lima Puluh Kota) evenly distributed across all quadrants (I-IV) while the Simmental pure breed (subpopulations BIB Lembang and BIBD Tuah Sakato) distributed only in the I quadrants and II quadrants. The highest genetic distance was identified between cattle in Agam and BIBD Tuah Sakato while the lowest genetic distance was identified between cattle in Agam and Lima Puluh Kota. Based on phenogram tree structure, the observed Simmental cattle could be classified into 2 clusters, subpopulations of BIBD Tuah Sakato and BIB Lembang that represent Simmental pure breed configured their own cluster as well as a subpopulation of Agam and Lima Puluh Kota that represent Simmental cross cattle configured the others. Key Words: Morphological, Genetic Distance, Simmental Cattle, Cross, West Sumatera
Polymorphism of IGF-1/RsaI and Carcass Percentage Variation in Sumba Ongole (SO) Cattle Agung, Paskah Partogi; Saputra, Ferdy
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.318 KB) | DOI: 10.33772/jitro.v9i2.21248

Abstract

Beef cattle have a different carcass percentage according to the breed. The percentage of carcass in livestock is an important indicator to determine the potential for meat obtained from cattle. IGF-1 is a gene from the growth gene family which is known to have an influence on the percentage of carcass. A total of 445 samples of Sumba Ongole cattle (156 males and 289 females) were used for genotyping using PCR-RFLP. A total of 237 carcass percentage data were obtained in this study. Data were performed using POPGENE version 1.32 and Microsoft Excel for genetic data and descriptive statistics, respectively. The intensively reared cattle have a higher carcass percentage than the cattle that were reared traditionally. The IGF-1/RsaI in the SO cattle was polymorphic with two alelles (C and T) and three genotype (CC, TT, and CT) but has low values of heterozygosity. The C allele frequency was 0.0034 and T allele frequency was 0.9966. Further studies in larger population are needed to validate the IGF1/RsaI as a candidate genetic marker in the SO cattle.
Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP Method Agung, Paskah Partogi; Perwitasari, Dyah; Farajallah, Achmad; Said, Syahruddin; Kaiin, Ekayanti Mulyawati; Saputra, Ferdy; Hermansyah, Alfandy; Kuswati, Kuswati; Susilorini, Tri Eko; Zein, Moch. Syamsul Arifin
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.87484

Abstract

The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
Co-Authors ACHMAD FARAJALLAH Dyah Perwitasari Erinaldi . F Saputra Ferdy Saputra Handrie . Hermansyah, Alfandy Indriawati . Kaiin, Ekayanti Mulyawati Kuswati Kuswati M. Ridwan Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Supraptono . Syahruddin Said Tri Eko Susilorini