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Melati, Putjha
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Agriculture, Biological Sciences & Forestry Chemistry Electrical & Electronics Engineering Health Professions Nursing

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OPTIMASI EKSTRAKSI DAUN NANGKA KUNING (Vincetoxicum villosum (Blume) Kuntze) DARI BERBAGAI PELARUT DAN METODE Oktoviani, Oktoviani; Taurina, Hilda; Triana, Dessy; Rahmawati, Suci; Sari, Yulita Gumala; Melati, Putjha; Sari, Delia Komala
JURNAL PENGELOLAAN LABORATORIUM SAINS DAN TEKNOLOGI Vol 2 No 1: Juni 2022
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/labsaintek.v2i1.22754

Abstract

Extraction of a plant in traditional medicine is a very crucial stage for the success of the treatment. For example, nangka kuning (Vincetoxicum villosum (Blume) Kuntze) which has been scientifically researched, its leaves are extracted with ethanol solvent using the maceration method. Meanwhile, by local people,nangka kuning leaves are processed by boiling using water. This attracted the attention of researchers to re-analyze the content of secondary metabolites in nangka kuning leaf extract made with different solvents and methods. Researchers carried out the extraction using water with heating (infusion), ethanol without heating (maceration) and chloroform without heating (maceration). The results obtained from the three types of extracts contained secondary metabolites of alkaloids in the three extracts. While steroids are only found in extracts with a water solvent. Then the tannins are found in the extract with water and ethanol as solvents. Negative results from the three extracts were shown for secondary metabolites of flavonoids and saponins. So it can be concluded that nangka kuning leaf extract which has the most optimal secondary metabolite is the extract using water as a solvent by heating.
UJI AKTIVITAS ANTIOKSIDAN, SITOTOKSISITAS DAN GC-MS EKSTRAK METANOL ALGA HIJAU Boergesenia Forbesii (HARVEY) FELDMANN DARI PANTAI PANJANG BENGKULU Melati, Putjha
JURNAL PENGELOLAAN LABORATORIUM SAINS DAN TEKNOLOGI Vol 1 No 1: Juni 2021
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.587 KB) | DOI: 10.33369/labsaintek.v1i1.15432

Abstract

Penelitian tentang aktivitas antioksidan, sitotoksisitas, dan GC MS ekstrak metanol alga hijau Boergesenia forbesii (Harvey) Feldmann telah dilaksanakan. Uji aktivitas antioksidan dilakukan dengan metode DPPH (1,1-diphenyl-2-picrylhydrazil). Uji sitotoksisitas terhadap Artemia salina Leach dilakukan dengan metode BSLT (Brine Shrimp Lethality Test). Identifikasi komponen kimia ekstrak dilakukan dengan menggunakan GC MS. Hasil uji menunjukkan bahwa ekstrak metanol alga hijau Boergesenia forbesii memiliki aktivitas antioksidan sangat lemah (IC50= 585,79ppm). Uji sitotoksisitas terhadap Artemia salena Leach menunjukkan bahwa ekstrak berkolerasi positif sebagai anti kanker (LC50 = 508,74 ppm). Berdasarkan analisis GC MS dari ekstrak metanol alga hijau Boergesenia forbesii menunjukkan 28 senyawa. Komponen utama dari ekstrak metanol alga hijau Boergesenia forbesii adalah Methyl Stearate, Phytol, Methyl Arachidonate, dan 9-Octadecenamide,(z)-. 
Co-Authors Delia Komala Sari, Delia Komala Dessy Triana Oktoviani, Oktoviani Sari, Yulita Gumala Suci Rahmawati Taurina, Hilda