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All Journal Indonesian Journal of Tropical and Infectious Disease Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Folia Medica Indonesiana
MOCHAMAD AMIN
Laboratorium Hepatitis Lembaga Penyakit Tropis Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Health Professions Medicine & Pharmacology Neuroscience Public Health

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HEPATITIS B SEROLOGY PROFILES ON CHILDREN AGED 1–13 YEARS OLD IN SUMENEP, MADURA Putera, Edward M; Marcia, Dian; Firdarini, Itja; Amin, Mochamad; Juniastuti, Juniastuti; Purwono, Priyo B; Utsumi, Takako; Lusida, Maria I
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.893 KB)

Abstract

Background: Hepatitis B virus (HBV) which was acquired during perinatal or childhood would promote hepatocellular carcinoma with even higher percentage than that which was acquired during adult age. That is why HBV represents a serious public health threat for children. HBV vaccination has been integrated into national expanded programme on immunization (EPI) since 1997. The aimof they study is to investigate the prevalence of HBV among children who were born after 1997 in Sumenep. Material and Methods: a total of 102 children who were born after 1997 were enrolled in this study. All children were admitted in the Emergency Room and Pediatric Ward of dr. H. Moh Anwar General Hospital for some reasons. Written informed consents were obtained from parents/guardians of all the children. Study protocol was reviewed and approved by the Ethics Committees. All of these cases were examined for hepatitis B surface antigen (HBsAg), antibody to HBsAg (Anti-HBs), and antibody to hepatitis B core antigen (Anti-HBc). Result and Discussion: Overall, 6 (5.88%) of 102 samples were positive for HBsAg, 51 (50.00%) of 102 samples were positive for anti-HBs, and 49 (48.04%) of 102 samples were positive for anti-HBc. All the children were born after 1997. Conclusion: HBsAg rate is still high even after universal vaccination program, acquired protective antibodies against hepatitis B surface antigen were sufficient, but there is a suspicion for occult hepatitis B infections (OBI). A further study to confirm OBI is needed.
HEPATITIS B SEROLOGY PROFILES ON CHILDREN AGED 1–13 YEARS OLD IN SUMENEP, MADURA Putera, Edward M; Marcia, Dian; Firdarini, Itja; Amin, Mochamad; Juniastuti, Juniastuti; Purwono, Priyo B; Utsumi, Takako; Lusida, Maria I
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.893 KB) | DOI: 10.20473/ijtid.v3i2.202

Abstract

Background: Hepatitis B virus (HBV) which was acquired during perinatal or childhood would promote hepatocellular carcinoma with even higher percentage than that which was acquired during adult age. That is why HBV represents a serious public health threat for children. HBV vaccination has been integrated into national expanded programme on immunization (EPI) since 1997. The aimof they study is to investigate the prevalence of HBV among children who were born after 1997 in Sumenep. Material and Methods: a total of 102 children who were born after 1997 were enrolled in this study. All children were admitted in the Emergency Room and Pediatric Ward of dr. H. Moh Anwar General Hospital for some reasons. Written informed consents were obtained from parents/guardians of all the children. Study protocol was reviewed and approved by the Ethics Committees. All of these cases were examined for hepatitis B surface antigen (HBsAg), antibody to HBsAg (Anti-HBs), and antibody to hepatitis B core antigen (Anti-HBc). Result and Discussion: Overall, 6 (5.88%) of 102 samples were positive for HBsAg, 51 (50.00%) of 102 samples were positive for anti-HBs, and 49 (48.04%) of 102 samples were positive for anti-HBc. All the children were born after 1997. Conclusion: HBsAg rate is still high even after universal vaccination program, acquired protective antibodies against hepatitis B surface antigen were sufficient, but there is a suspicion for occult hepatitis B infections (OBI). A further study to confirm OBI is needed.
DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1302.693 KB) | DOI: 10.20473/ijtid.v7i5.7275

Abstract

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.
Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer Mastutik, Gondo; Rahniayu, Alphania; , Isnin Anang Marhana; Amin, Mochamad; Trianto, Heru Fajar; I'tishom, Reny
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. In this study, new primers designed using the semi-nested polymerase chain reaction (PCR) method were utilized to identify MAGE-A11 and MAGE-A12 expressions in specimens collected from core biopsy, forcep biopsy, and bronchoalveolar lavage. 2. The histopathological analysis revealed positive expressions of MAGE-A11 and MAGE-A12 in specimens diagnosed with non-small cell lung cancer (NSCLC) as well as in specimens with no malignant cells. 3. This study provides evidence indicating that the detection of messenger ribonucleic acid (mRNA) of MAGE-A11 and MAGE-A12 by nested reverse transcription PCR can improve the accuracy of lung cancer diagnosis. Abstract The melanoma antigen gene (MAGE) belongs to the group of cancer-testis antigens that are exclusively expressed in germ cells but may be re-expressed in cancer cells. The highly expressed MAGE-A subfamily in lung cancer may potentially be a diagnostic and prognostic biomarker. This study aimed to identify MAGE-A11 and MAGE-A12 expressions in lung tumors obtained from core biopsy, forceps biopsy, and bronchoalveolar lavage specimens. A cross-sectional observational study was conducted on 90 patients clinically diagnosed with lung tumors. These patients received core biopsy, forceps biopsy, and bronchoalveolar lavage interventions after ethical approval was obtained. The complementary deoxyribonucleic acid (cDNA) quality was assessed by the polymerase chain reaction (PCR) of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The assessment was performed to ascertain if all specimens exhibited positive PCR amplification of the GAPDH gene. MAGE-A11 and MAGE-A12 were identified through a semi-nested reverse transcription PCR. The positive results were detected by measuring the PCR products, with MAGE-A11 and MAGE-A12 at base pairs (bp) of 858 and 496 in the first and second rounds, respectively. The expressions of MAGE-A11 and MAGE-A12 were observed in 3 (3.33%) and 40 (44.44%) out of 90 specimens, respectively. The prevalence rate of non-small cell lung cancer (NSCLC) was 31.11% (28/90). Among these cases, 3.57% (1/28) showed the expression of MAGE-A11, while 32.14% (9/28) exhibited the expression of MAGE-A12. Sixty-two (68.89%) out of 90 patients were diagnosed with no tumor cell malignancy. Out of 62 cases, 2 (3.23%) exhibited the expression of MAGE-A11, while 31 (50%) demonstrated the expression of MAGE-A12. MAGE-A11 and MAGE-A12 were detected in NSCLC and certain specimens with a pathological diagnosis that indicated the absence of malignant cells. In conclusion, MAGE A11 and MAGE A12 have potential markers to improve the pathological diagnosis of lung cancer. Further investigation is necessary to explore the expression of MAGE-A in correlation with lung cancer progression.
Characterization of soil pathogenic fungi in the rhizosphere of siwalan palm (Borassus flabellifer L.) Nurtjahyani, Supiana Dian; Sulistyaningrum, Heny; Sulistiono; Amin, Mochamad; Tegar Kurnia, Dwi; Dhiaulhaq, M. Rafi; Mustofa, Ali
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2156

Abstract

Soil-borne fungal pathogens represent a persistent threat to plant health due to their ability to colonize the rhizosphere and infect root systems, yet information on their diversity in non-industrial palm species remains limited. Siwalan (Borassus flabellifer L.) is a drought-tolerant palm of ecological and socio-economic importance, but its rhizospheric fungal communities have not been systematically characterized. This study aimed to (1) isolate soil-borne fungi from the rhizosphere of siwalan and (2) identify potential pathogenic fungi based on macroscopic and microscopic morphological characteristics. Rhizosphere soil samples were collected at depths of 5–20 cm and processed using the soil dilution plate method. Fungal isolates were cultured on potato dextrose agar (PDA), purified through subculturing, and identified using colony morphology and microscopic structures observed through slide culture and lactophenol cotton blue staining. Data were analyzed descriptively by comparing fungal traits with standard taxonomic determination keys. Five soil-borne fungal taxa were successfully isolated and identified, namely Aspergillus flavus, Trichoderma longibrachiatum, Aspergillus oryzae, Aspergillus caelatus, and Aspergillus niger. The isolates exhibited distinct differences in colony pigmentation, growth rate, hyphal structure, vesicle morphology, and conidial characteristics. Among them, A. niger showed the fastest radial growth and highest sporulation intensity, whereas T. longibrachiatum and A. oryzae exhibited relatively slower growth. These morphological variations indicate functional diversity and differing adaptive strategies among fungi inhabiting the siwalan rhizosphere. This study provides the first systematic morphological baseline of soil-borne fungi associated with the rhizosphere of B. flabellifer. The findings contribute novel information on fungal diversity in an underexplored palm species and highlight the importance of early fungal characterization as a foundation for future molecular identification, pathogenicity testing, and the development of sustainable disease management strategies for siwalan cultivation systems.
Co-Authors , Isnin Anang Marhana Ali Mustofa Alphania Rahniayu Dhiaulhaq, M. Rafi Dian Marcia, Dian Edward M Putera, Edward M Gondo Mastutik Heny Sulistyaningrum I'tishom, Reny Itja Firdarini, Itja Juniastuti Juniastuti Maria I Lusida, Maria I Maria Inge Lusida Poernomo Boedi Setiawan Priyo B Purwono, Priyo B Priyo Budi Purwono Retno Handajani Ruslan, S. Eriaty N. SOETJIPTO . Sulistiono Supiana Dian Nurtjahyani Takako Utsumi Tegar Kurnia, Dwi Trianto, Heru Fajar Ulfa Kholili Ummi Maimunah Wungu, Citrawati Dyah Kencono