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All Journal Microbiology Indonesia Indonesian Journal of Tropical and Infectious Disease Sari Pediatri J-Dinamika: Jurnal Pengabdian Kepada Masyarakat
Priyo Budi Purwono
Institute Of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department Of Microbiology, Faculty Of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia Indonesia-Japan Coll
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Gambaran Terapi dan Luaran Infeksi Saluran Kemih oleh Bakteri Penghasil Extended Spectrum Beta Lactamase pada Anak di RSUD Dr. Soetomo Surabaya Ahmad Muhajir; Priyo Budi Purwono; Samsriyaningsih Handayani
Sari Pediatri Vol 18, No 2 (2016)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp18.2.2016.111-6

Abstract

Latar belakang. Kasus infeksi saluran kemih oleh bakteri penghasil extended spectrum beta-lactamase (ESBL) dilaporkan sudah banyak terjadi di dunia. Informasi terkait ESBL di Indonesia sangat sedikit, terutama pada pasien anak.Tujuan. Mengkaji gambaran terapi serta luaran pasien infeksi ESBL di RSUD Dr. Soetomo Surabaya periode Januari-Juni 2014.Metode. Penelitian observasional dengan studi deskriptif, digunakan data rekam medis pasien anak RSUD Dr. Soetomo Surabaya periode Januari-Juni 2014. Variabel penelitian meliputi bakteri, uji sensitivitas, terapi serta status luaran pasien.Hasil. Pasien anak berjumlah 56, terbanyak penyebab ISK Klebsiella pneumoniae (60,7%). Uji sensitivitas tertinggi meropenem (90,5%). Pilihan terapi definitif meropenem (25%), status luaran membaik 78,57%.Kesimpulan. Meropenem merupakan pilihan terapi infeksi ESBL pada pasien dengan keadaan klinis membaik. Kontrol penggunaan antibiotik secara rasional dibutuhkan untuk pencegahan infeksi ESBL
The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia MOCHAMAD AMIN; JUNIASTUTI .; TAKAKO UTSUMI; YOSHIHIKO YANO; MOCHAMAD YUSUF; MOCHAMMAD THAHA; PRIYO BUDI PURWONO; RETNO HANDAJANI; SOETJIPTO .; HAK HOTTA; YOSHITAKE HAYASHI; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.539 KB) | DOI: 10.5454/mi.6.4.5

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.
ANTIMICROBIAL ACTIVITY OF PINEAPPLE (ANANAS COMOSUS L. MERR) EXTRACT AGAINST MULTIDRUG-RESISTANT OF PSEUDOMONAS AERUGINOSA: AN IN VITRO STUDY Zharfan, Rahmat Sayyid; Purwono, Priyo Budi; Mustika, Arifa
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 5 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.228 KB) | DOI: 10.20473/ijtid.v6i5.4159

Abstract

Pseudomonas aeruginosa is the main cause of nosocomial infection which is responsible for 10% of hospital-acquired infection. Pseudomonas aeruginosa tends to mutate and displays potential for development of antibiotic resistance. Approximately, 10% of global bacterial isolates are found as Multidrug-resistant Pseudomonas aeruginosa. Pseudomonas aeruginosa have a quite tremendous severity index, especially on pneumonia and urinary tract infections, even sepsis, which 50% mortality rate. Pineapple (Ananas comosus L. Merr) has antimicrobial properties. The active antimicrobial compounds in Ananas comosus L. Merr include saponin and bromelain. This research aims to find the potency of antimicrobial effect of pineapple (Ananas comosus L. Merr) extract towards Multidrug-resistant Pseudomonas aeruginosa. Multidrug-resistant Pseudomonas aeruginosa specimen is obtained from patient's pus in orthopaedic department, Dr Soetomo Public Hospital, Surabaya. Multidrug-resistant Pseudomonas aeruginosa specimen is resistant to all antibiotic agents except cefoperazone-sulbactam. This research is conducted by measuring the Minimum Inhibitory Concentration (MIC) through dilution test with Mueller-Hinton broth medium. Pineapple extract (Ananas comosus L. Merr.) is dissolved in aquadest, then poured into test tube at varying concentrations (6 g/ml; 3 g/ml; 1.5 g/ml; 0.75 g/ml, 0.375 g/ml; and 0.1875 g/ml). After 24 hours' incubation, samples are plated onto nutrient agar plate, to determine the Minimum Bactericidal Concentration (MBC). The extract of pineapple (Ananas comosus L. Merr) has antimicrobial activities against Multidrug-resistant Pseudomonas aeruginosa. Minimum Inhibitory Concentration (MIC) could not be determined, because turbidity changes were not seen. The Minimum Bactericidal Concentration (MBC) of pineapple extract (Ananas comosus L. Merr) to Multidrug-resistant Pseudomonas aeruginosa is 0.75 g/ml. Further study of in vivo is needed.
DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1302.693 KB) | DOI: 10.20473/ijtid.v7i5.7275

Abstract

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.
Pemberdayaan Guru Yayasan Pendidikan Al Khairaat Yogyakarta pada Penanggulangan Antimicrobial Resistance: dari UNAIR untuk Indonesia Wahyunitisari, Manik Retno; Koendhori, Eko Budi; Endraswari, Pepy Dwi; Purwono, Priyo Budi; Juniastuti, Juniastuti; Wiqoyah, Nurul; Sardjono, Lindawati Alim; Vermasari, Naritha; Muna, Nafdzu Makhmudatul; Setiawan, Firman; Lindarto, Wira Widjaya; Widyatama, Fikri Sasongko
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 3 (2024): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v9i3.5481

Abstract

Antibiotics are compounds created by microorganisms to live by destroying their competitors. Antibiotics are commonly used without a prescription to treat symptoms caused by non-bacterial infections. Antibiotic resistance is a global problem. Continuous education is needed by agents of change to overcome this problem. Community service collaboration between the Department of Microbiology-Parasitology, FK Unair, and the Department of Microbiology, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University with partnerships for the goals teachers from the Al Khairaat Yogyakarta educational foundation, aim to provide an understanding of the proper use of antibiotics. The initial target of transfer of knowledge was successfully measured by increasing participants' post-test results.
Co-Authors Ahmad Muhajir Arifa Mustika Eko Budi Koendhori, Eko Budi Fikri Sasongko Widyatama HAK HOTTA Juniastuti Juniastuti Lindarto, Wira Widjaya Maria Inge Lusida MARIA INGE LUSIDA MOCHAMAD AMIN MOCHAMAD AMIN Mochamad Yusuf Alsagaff MOCHAMMAD THAHA Muna, Nafdzu Makhmudatul Nurul Wiqoyah, Nurul Pepy Dwi Endraswari, Pepy Dwi Poernomo Boedi Setiawan Retno Handajani Ruslan, S. Eriaty N. Samsriyaningsih Handayani Sardjono, Lindawati Alim Setiawan, Firman SOETJIPTO . SOETJIPTO . TAKAKO UTSUMI Ulfa Kholili Ummi Maimunah Vermasari, Naritha Wahyunitisari, Manik Retno Wungu, Citrawati Dyah Kencono YOSHIHIKO YANO YOSHITAKE HAYASHI Zharfan, Rahmat Sayyid