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All Journal Jurnal NERS Keluwih: Jurnal Kesehatan dan Kedokteran Keluwih: Jurnal Sains dan Teknologi
Mariana Wahjudi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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Ekstrak Kasar Isolat Bacillus sp Sebagai Agen Peluruh Biofilm Gultom, Mangihot Tua; William Thamrin; Mariana Wahjudi; Suhartono
Keluwih: Jurnal Kesehatan dan Kedokteran Vol. 4 No. 2 (2023): Keluwih: Jurnal Kesehatan dan Kedokteran (June)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/kesdok.V4i2.5676

Abstract

Abstract–Biofilms play an important role in the distribution and pathogenicity of bacteria. In our previous research, we demonstrated that our isolated Bacillus sp. (collection of Microbiology Lab, Universitas Surabaya) exhibited in vitro antibiofilm activity. Bacilli generally produce cellulase, which is one of the enzymes responsible for bacterial biofilm degradation. Based on this fact, this study aimed (1) to determine whether or not the crude extract of our Bacillus sp. isolate is able to degrade biofilms formed by Escherichia coli, Staphylococcus aureus, and water-crane bacteria, and (2) to determine whether such crude extract contains active cellulase. The research steps began with the preparation of Bacillus sp. crude extract, biofilm degradation assay, cellulase activity assay on CMC Agar, and cellulase detection by SDS-PAGE and electrophoresis zymogram. The crude extract of Bacillus sp. effectively degraded the biofilm 30 min after contact. The crude extract decreased biofilms of Escherichia coli 23,56%, Staphylococcus aureus 27,78%, and water-crane bacteria 37,98%. Based on the zymogram result, it was shown that the crude extract contained active cellulase, with a size of approximately 35 kDA. It was concluded that the crude extract of Bacillus sp. has potential as an anti-biofilm agent and exhibits active cellulase activity. Keywords: Bacillus sp., crude extract, biofilm, SDS-PAGE, zymogram Abstrak–Biofilm mempunyai peran penting dalam penyebaran dan sifat patogenitas bakteri patogen. Pada penelitian sebelumnya telah dibuktikan bahwa isolat Bacillus sp. (koleksi Laboratorium Mikrobiologi, Universitas Surabaya) mempunyai aktivitas anti-biofilm secara in vitro. Bacillus umumnya menghasilkan selulase sedangkan selulase diketahui dapat meluruhkan biofilm bakteri. Berdasarkan hal di atas, penelitian ini bertujuan (1) untuk mengetahui apakah ekstrak kasar isolat Bacillus sp. ini dapat meluruhkan biofilm Escherichia coli dan Staphylococcus aureus, dan bakteri air keran; dan (2) untuk mengetahui apakah dalam ekstrak kasar terdapat selulase. Metode penelitian ini meliputi penyiapan ekstrak kasar sel Bacillus sp., pengujian aktifitas meluruhkan biofilm, uji aktivitas selulase pada Agar CMC, dan analisis keberadaan aktivitas selulase pada ekstrak kasar Bacillus sp. menggunakan SDS-PAGE dan elektroforesis zymogram. Hasil penelitian menunjukkan bahwa ektrak kasar isolat Bacillus sp. bekerja paling baik pada perlakuan 30 menit setelah kontak dengan biofilm bakteri. Pemberian ekstrak kasar dapat menurunkan biofilm Escherichia coli sebesar 23,56%; dan biofilm bakteri Staphylococcus aureus sebesar 27,78%, dan biofilm bakteri air keran sebesar 37,98%. Berdasarkan hasil zymogram, telihat bahwa ekstrak kasar isolat Bacillus sp. memiliki selulase aktif dengan ukuran sekitar 35 kDa. Dapat disimpulkan bahwa ekstrak kasar isolat Bacillus sp. berpotensi sebagai agen peluruh biofilm dan memiliki selulase aktif. Kata kunci: Bacillus sp., ekstrak kasar, biofilm, SDS-PAGE, zymogram
Biokomposit Hidrogel dengan Ekstrak Centella asiatica sebagai Penutup Luka Dominique Gian Leonardo; Stephanie Singajaya; Dinda Fluor Agustin; Mariana Wahjudi
Keluwih: Jurnal Sains dan Teknologi Vol. 3 No. 1 (2022): Keluwih: Jurnal Sains dan Teknologi (February)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/saintek.v3i1.4907

Abstract

Abstract—When wounds occur on skin, cell regeneration and healing process will occur. Imperfect cell regeneration will end up leaving ugly scars. Any open wounds will have a high chance of being infected, further slowing the healing processes. The bacteria involved in wound infection are as follow: Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. The usage of current medical adhesive to cover wound has many implications: cannot be used multiple times and untuk may end up resulting in MARSI (Medical Adhesive-Related Skin Injuries). To solve those problems, this research aims to find an alternative wound dressing that are able to solve the problems mentioned earlier. The synthesis of biocomposite hydrogel consists of bacterial cellulose mixed with gelatin with the crosslinker agent glutaraldehyde. The mixture is then given the wild herbs Centella asiatica extract as an active ingredient. It has been found that the best formulation consists of using 10% gelatine: bacterial cellulose paste with the ratio 200:1 with the total glutaraldehyde concentration of 1%. Furthermore, Centella asiatica 10% are not able to penetrate the human model skin, and did not exhibit any antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Keywords: biocomposite hydrogel, nata de coco, bacterial cellulose, gelatine, centella asiatica extract, wound dressing Abstrak—Ketika luka terjadi pada kulit, regenerasi sel dan proses penyembuhan akan terjadi. Regenerasi sel yang tidak sempurna dapat menghasilkan bekas luka yang kurang enak dipandang. Selain itu, luka terbuka berisiko terinfeksi oleh patogen sehingga penyembuhan akan terhambat. Bakteri yang berperan pada infeksi luka adalah Pseudomonas aeruginosa, Escherichia coli dan Staphylococcus aureus. Penggunaan medical adhesive untuk menutup luka punya banyak kekurangan yaitu penggunaannya tidak bisa dilakukan berulang dan dapat menyebabkan MARSI (Medical Adhesive-Related Skin Injuries), yaitu berbagai komplikasi dan luka pada kulit yang ditempeli dengan produk tersebut. Untuk mengatasi pemasalahan tersebut, penelitian ini bertujuan untuk mendapatkan alternatif penutup luka terbaru yang dapat mengatasi permasalahan yang telah disebutkan. Pembuatan hidrogel biokomposit terdiri dari selulosa bakteri yang direaksikan dengan gelatin menggunakan agen crosslinker glutaraldehida. Campuran kemudian ditambahkan bahan aktif dari ekstrak tanaman liar Centella asiatica. Didapatkan formulasi terbaik menggunakan perbandingan 10% gelatin:pasta selulosa bakteri = 200:1 dengan konsentrasi total glutaraldehida sebanyak 1%. Selain itu, ekstrak Centella asiatica 10% juga tidak dapat mempenetrasi model kulit manusia, serta tidak memiliki aktivitas antibakteri baik pada Escherichia coli, Pseudomonas aeruginosa, dan Staphylococcus aureus. Kata kunci: hidrogel biokomposit, nata de coco, selulosa bakteri, gelatin, ekstrak centella asiatica, penutup luka
Stenotrophomonas maltophilia, Bakteri Resisten Merkuri pada Limbah Pertambangan Logam Gultom, Mangihot Tua; Shienen Sisilia; Mariana Wahjudi
Keluwih: Jurnal Sains dan Teknologi Vol. 3 No. 1 (2022): Keluwih: Jurnal Sains dan Teknologi (February)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/saintek.v3i1.4910

Abstract

Abstract—Mercuric-resistant bacterium isolate coded 22 was previously obtained from metal mining (tailing) wastewater samples. From the total 24 bacteria which had been isolated, isolate 22 exhibited the highest resistance toward high concentration of Hg. In this paper it is reported results of 16S rDNA-based identification of isolate 22 as well as its growth and resistance characteristics when growing on Mercuric containing Nutrient Broth medium. Isolate 22 is closely related to the bacterium, Stenotrophomonas maltophilia (identity 99% and query covered 90%). Isolate 22 grew very well in the NB-Hg medium containing Hg concentration of 50 and 100 ppm. Increasing Hg concentration clearly lengthened lag phase although cell quantity (culture turbidity, OD600) at the initial phase were the same. Keywords: isolate22, Hg, identification, sequencing, 16S rRNA gene Abstrak—Isolat 22 adalah kode isolat bakteri resisten logam Merkuri (Hg) yang didapatkan pada sampel air limbah pertambangan logam (tailing). Isolat 22 adalah isolat yang paling berdaya tahan tinggi terhadap Hg, dari total 24 isolat yang berhasil diisolasi. Pada paper ini akan dilaporkan hasil identifikasi spesies bakteri isolat 22 menggunakan teknik PCR - sekuensing gen 16S rRNA dan karakter pertumbuhan dan resistensi isolat 22 terhadap Hg. Dari hasil sekuensing 16S rRNA, diketahui bahwa isolat 22 sangat dekat kekerabatannya dengan spesies Stenotrophomonas maltophilia (identity 99% dan query covered 90%). Isolat 22 tumbuh dengan baik pada medium NB-Hg dengan kadar Hg 50 dan 100 ppm. Semakin tinggi kadar Hg dalam medum, semakin lama fase lag, walaupun jumlah sel pada medium di fase awal sama. Kata kunci: isolat 22, hg, identifikasi, sekuensing, gen 16S rRNA
The Isoflavone Contents of Devon 1 Soybeans during Fermentation and Processing into Soybean-Tempeh Steamed Buns Amanda Villelie Sudarmin; Christina Mumpuni Erawati; Tjandra Pantjajani; Stephanie Belinda Wijaya; Pandu Salim Hanafi; Mariana Wahjudi
Keluwih: Jurnal Kesehatan dan Kedokteran Vol. 7 No. 1 (2025): Keluwih: Jurnal Kesehatan dan Kedokteran (December)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/kesdok.V7i1.7929

Abstract

Abstract—The soybean Devon 1 variety is a superior variety developed in Indonesia. The isoflavone content makes this soybean a top choice for a functional food. However, fermenting soybeans into tempeh and processing them into other products can affect the final isoflavone content. The objective of this study was to analyze the total isoflavone and its derivatives content in Devon 1 soybeans, soybean tempeh, soybean tempeh flour, and buns made from tempeh flour. The content of each isoflavone type was determined by high-performance liquid chromatography at λ249 and λ260 nm wavelengths. The buns were made from tempeh flour with variations of tempeh flour substitution of 0, 10, 20, and 30%. The results showed that the fermentation of Devon 1 soybeans and further processing of tempeh significantly altered the isoflavone components and content. The concentrations of daidzin and genistin in tempeh were decreased, while daidzein and genistein were increased. Further processing of tempeh into flour increased all isoflavone levels significantly, which might be caused by the isoflavone transformation during drying and enzymatic activity. Substitution of wheat flour with soybean tempeh flour increased these four isoflavones content of the buns. Therefore, tempeh flour made from Devon 1 soybeans has the potential to be used as a functional food ingredient rich in bioactive compounds that promote health. Keywords: flour, heat, isoflavone aglycones, isoflavone glycoside, soybean tempeh Abstrak—Kedelai varietas Devon 1 merupakan varietas unggul yang dikembangkan di Indonesia. Kandungan isoflavonnya menjadikan kedelai ini sebagai pilihan utama untuk pembuatan pangan fungsional. Namun, proses fermentasi kedelai menjadi tempe, serta pengolahan lebih lanjut menjadi berbagai produk turunan dapat memengaruhi kandungan akhir isoflavonnya. Penelitian ini bertujuan untuk menganalisa kandungan total isoflavon dan turunannya pada kedelai Devon 1, tempe kedelai, tepung tempe kedelai, serta bakpao yang dibuat dari tepung tempe. Kandungan masing-masing jenis isoflavon diuji menggunakan kromatografi cair kinerja tinggi pada panjang gelombang λ249 dan λ260 nm. Adapun bakpao dibuat dari tepung tempe dengan variasi substitusi tepung tempe sebesar 0, 10, 20, dan 30%. Hasil penelitian ini menunjukkan bahwa fermentasi kedelai Devon 1 dan pengolahan tempe lebih lanjut secara signifikan mengubah komponen dan kandungan isoflavon. Kandungan daidzin dan genistin dalam tempe menurun, sedangkan kandungan daidzein dan genisteinnya mengalami peningkatan. Pengolahan tempe menjadi tepung lebih lanjut meningkatkan seluruh kandungan isoflavon secara signifikan, yang kemungkinan disebabkan oleh transformasi isoflavon selama proses pengeringan dan aktivitas enzimatis. Substitusi tepung terigu dengan tepung tempe meningkatkan kandungan keempat jenis isoflavon pada bakpao. Dengan demikian, tepung tempe kedelai Devon 1 berpotensi digunakan sebagai bahan pangan fungsional yang kaya akan senyawa bioaktif untuk menunjang kesehatan. Kata kunci: aglikon isoflavon, glikosida isoflavon, pemanasan, tempe kedelai, tepung
GABRA6 and SLC6A4 genotypes are correlated with the fasting blood glucose and physical fitness in the seemingly healthy young adults Viskasari P Kalanjati; Mariana Wahjudi; Kusuma E. Purwantari; Visuddho; Peppy Nawangsasi; Rury T. Oktariza; Krisnawan A. Pradana; Dzanuar Rahmawan; Bambang E. Suwito
Jurnal Ners Vol. 18 No. 1 (2023): MARCH 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jn.v18i1.43770

Abstract

Introduction: We examined environmental and genetic factors potentially correlated with the blood glucose and physical fitness in Kediri, East Java, Indonesia to elucidate the predominant cardiovascular disease risk. Methods: This was a cross-sectional study on 68 adults aged 18-22 years. Direct interview was conducted to get socio-economy,  daily diets (rice/ R, snacks and sweets/ SS, veggies and fruits/ VF), daily activity (locomotors and non-locomotors) data. Peripheral blood-GABRA6 and SLC6A4 polymorphisms, fasting blood glucose (FBG), blood pressure, 3 fitness parameters (1 min push-up, 3 minutes step up, pulse rate after 3 minutes step-up) were measured by 2 independent and pre-trained medical doctors. Kruskal-Wallis, Chi-Square, and eta-coefficient tests were used to seek differences between genotypes and correlation strength between variables, respectively (significant if p<0.05). Results: Both gene's genotypes showed significant correlations with the FBG (p=0.042, p=0.013, respectively); GABRA6 polymorphism significantly correlated with SU (p=0.033). There was a significant difference in the FBG between 2 VF subgroups (p=0.02). Strongest association was found between FBG and SLC6A4 (η=0.3); PR and GABRA6 (η=0.168), PU and SLC6A4 (η=0.38); SU and Locomotors (η=0.237). Conclusions: In these seemingly healthy young adults, daily diets and the genotypes of both genes have a good association with the FBG and physical fitness levels.
Co-Authors Amanda Villelie Sudarmin Bambang E. Suwito Christina Mumpuni Erawati Dinda Fluor Agustin Dominique Gian Leonardo Dzanuar Rahmawan Gultom, Mangihot Tua Krisnawan A. Pradana Kusuma E. Purwantari Pandu Salim Hanafi Peppy Nawangsasi Rury T. Oktariza Shienen Sisilia Stephanie Belinda Wijaya Stephanie Singajaya Suhartono Tjandra Pantjajani Viskasari P. Kalanjati Visuddho William Thamrin