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All Journal HAYATI Journal of Biosciences Jurnal Biologi Udayana AL KAUNIYAH Jurnal Matematika & Sains Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Chemistry Medika Kartika : Jurnal Kedokteran dan Kesehatan 3BIO: Journal of Biological Science, Technology and Management Berkala Penelitian Hayati
Anggraini Barlian
Biology Study Program, School Of Life Sciences And Technology Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF) Anggraini Barlian; Rini Agustin; Ramadhani Eka Putra
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 2 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.2.1

Abstract

The skin damage induced by UV irradiation can cause photoaging as a consequence of reactive oxygen species (ROS) generation. Propolis is one of the most abundant natural product sources of polyphenols and derived from various plant resins collected by honeybees. This study aimed to determine the due administration of the propolis nanoemulsion (NEP) as an anti-photoaging by observing the concentration of intracellular ROS and lipid peroxides in human dermal fibroblast (HDF) by exposure to UVB. The cytotoxic of NEP was tested by the MTT assay, intracellular ROS was measured using the DCFDA assay, lipid peroxide products were analyzed by the TBARS assay and cell nuclei was observed by DAPI staining. PSA analysis revealed that the sizes of NEP were about 131.9 to 131 nm. The optimum concentration of NEP was 0,1μg / ml. ROS production in HDF treatment of NEP significantly decreases (p <0.05). Lipid peroxide products showed the same pattern as ROS concentration. The lipid peroxide concentration in the treatment NEP significantly decreases (p<0.05). In conclusion, the propolis in NEP acts as an antioxidant and has the potential to reduce the production of ROS and lipid peroxides caused by exposure to UVB.
Aging Process in Dermal Fibroblast Cell Culture of Green Turtle (Chelonia mydas) Anggraini Barlian; Yemima D Riani
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 2 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.2.2

Abstract

Senescence in green turtle is an interesting process to study because until now no clear explanation has been established about senescence at cellular or molecular level in this species. One of common markers used for detecting senescence is telomere shortening. Reduced telomerase activity may also cause senescence. The aims of this research are to establish and identify dermal fibroblast cell culture from green turtle and also to compare telomere length and telomerase activity from the cells subculture 5 and 14. Cells were identified with Rabbit Anti-Vimentin Polyclonal Antibody and Goat Polyclonal Antibody using confocal microscope. Telomere length was obtained using TeloTAGGG Telomere Length Assay (Roche), while telomerase activity was obtained using TeloTAGGG Telomerase PCR ElisaPlus. Primary cell culture from green turtle skin showed fibroblastic morphology and immunocytochemistry results using vimentin antibody proved that the culture was fibroblast cell. Measurement of telomere length and telomerase activity showed that telomere length and telomerase activity of subculture 14 was greater than subculture 5. However, based on morphology, green turtle fibroblast skin cell culture showed senescent morphology. Therefore, possible aging mechanism that the green turtle fibroblast skin cell culture underwent did not go through both telomere shortening and reduced telomerase activity.
The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells Imam Rosadi; Karina Karina; Iis Rosliana; Siti Sobariah; Irsyah Afini; Tias Widyastuti; Anggraini Barlian
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5637.848 KB) | DOI: 10.21705/mcbs.v3i1.43

Abstract

Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment.Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC. Key words: human ADSC, PRP, L-ascorbic acid, proliferation, chondrogenesis
EFEK DIKLOFENAK DAN VCO TERHADAP EKSPRESI RESEPTOR EP3 PADA TIKUS STRAIN WISTAR ADIKSI NIKOTIN KONDISI DEPENDENCE Dian Anggraeny; Anggraini Barlian; Kusnandar Anggadiredja
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 1 No 1 (2017): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.12 KB)

Abstract

Perilaku ketergantungan rokok timbul akibat adanya nikotin sebagai komponen psikoaktif utama. Salah satu mekanisme yang diduga berperan adalah proses fisiologis yang melibatkan metabolisme asam arakhidonat yang dapat diamati melalui perubahan ekspresi gen reseptor epiprostanoid (EP3) di otak. Penelitian ini bertujuan untuk mempelajari ekspresi reseptor EP3 pada model tikus adiksi nikotin kondisi dependence, dan yang diberi pre-treatment dengan diklofenak dan VCO. Tikus-tikus secara random dibagi menjadi: kelompok 1 (N) diberi nikotin saja (0,5mg/kg i.p.), kelompok 2 (Nd) diberi pre-treatment diklofenak (3,2mg/kg i.p.) dan kelompok 3 (NVCO) diberi pre-treatment VCO (5ml/kg p.o.) sebelum pemberian nikotin. Pengukuran ekspresi dilakukan saat tikus telah mengalami ketergantungan (dependence) menggunakan metode Conditioned Place Preference (CPP). Tikus-tikus diambil bagian otak hipokampus dan bulbus olfaktoriusnya, kemudian dilakukan isolasi protein, elektroforesis dan ditransfer ke membran PVDF menggunakan metode Western Blotting. Selanjutnya dilakukan teknik Enhanced Chemiluminescence (ECL) menggunakan antibodi primer rabbit polyclonal anti reseptor EP3 (Abcam) dan antibodi rabbit polyclonal anti-actin (Abcam) sebagai kontrol internal. Pita yang terbentuk didedahkan pada film dan dihitung dengan scion image secara semi kuantitatif. Ekspresi reseptor EP3 paling tinggi ditemukan pada kelompok tikus yang hanya diberi nikotin, dan menurun pada kelompok tikus yang diberi diklofenak dan VCO. Pada sampel dari tikus yang diberi VCO, teramati penurunan ekspresi reseptor EP3 yang sebanding dengan yang diberi diklofenak. Paparan nikotin secara berulang akan meningkatkan ekspresi reseptor EP3 yang dapat ditekan dengan pemberian diklofenak. Penurunan ekspresi reseptor EP3 dengan pemberian VCO memiliki pola yang mirip dengan pemberian diklofenak. DOI : 10.35990/mk.v1n1.p15-31
3D Biosilica Scaffolds from Melophlus sarasinorum and Xestospongia testudinaria Indonesian Sponges are Biocompatible for Cell Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cell in Bone Tissue Engineering Soraya Rahmanisa; Ekavianty Prajatelistia; Indra Wibowo; Anggraini Barlian
The Indonesian Biomedical Journal Vol 14, No 4 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i4.1895

Abstract

BACKGROUND: Biosilica derived from Indonesian marine sponge Melophlus sarasinorum and Xestospongia testudinaria is one of the biomaterials that can be developed together with synthetic polymer as a composite. Poly E-caprolactone (PCL) used as a composite role as an osteoconductive material together with biosilica and also tailored the slow rate of degradation in the body. This study aimed to create a biocompatible biosilica-based scaffold and supports osteogenic differentiation of human Wharton's Jelly mesenchymal stem cell (hWJ-MSCs).METHODS: Biosilica was extracted from M. sarasinorum and X. testudinaria with the acid digestion method. Scaffold was prepared using the salt leaching method. The composite scaffolds were made from seven different biosilica extract and PCL. All of the scaffolds were tested for the cell morphology, Fourier-transform infrared spectroscopy (FTIR), immunocytochemistry, and cytotoxicity.RESULTS: Composite scaffolds of 50% M. sarasinorum and X. testudinaria increased the cell viability and supported the cell growth within 14 days, whereas the osteogenic differentiation can be seen by the presence of collagen type 1 in day 12 based on immunocytochemistry result.CONCLUSION: The biosilica scaffolds from PCL+50% M. sarasinorum and PCL+50% X. testudinaria were promising 3D scaffolds for potential application in bone tissue engineering. In conclusion, this study shows evidence for the osteogenic differentiation of hWJ-MSC, which might be developed for bone tissue engineering.KEYWORDS: sponge, biosilica, scaffold, osteogenesis, stem cell
Isolation of Native Plant-Derived Exosome-like Nanoparticles and Their Uptake by Human Cells Diah Ratnadewi; Christofora Hanny Widjaja; Anggraini Barlian; Rizka Musdalifah Amsar; Ika Dewi Ana; Atik Choirul Hidajah; Hari Basuki Notobroto; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.182-192

Abstract

The exosome is a nano-sized vesicular particle commonly secreted from eukaryotic cells to extracellular space. Exosome functions in cellular communication, nutrients or bioactive compounds delivery, and cellular immunity. It is believed that plant-derived exosome-like particle (PDEN) potential to deliver nano-bioactive compounds to the human body. As Indonesia is one of the herbal centers of the world, we are encouraged to develop natural richness for human welfare. The study aimed to verify various methods to isolate exosomes from the fresh ginger rhizome and quina cells and examine the ginger PDENs' capacity to internalize in vitro human cells. The PDENs isolation was carried out by centrifugation and precipitation. The particles were observed through a transmission electron microscope, dynamic light scattering, and particle size analysis. Differential ultracentrifugation combined with PEG6000 provided a better size range of particles. Nanoparticles tracking analysis performed the concentration of particles within a specific size range. Ginger and quina PDENs size ranged from 40-650 nm, and the concentration was 3.6 × 107 and 2.8 × 106, respectively. The PKH67-labelled ginger PDENs were taken up by human dermal fibroblast and human Wharton's jelly mesenchymal stem cells after 30 minutes and 21 hours. DPPH assays revealed that ginger PDENs have antioxidant activity, indicating the presence of some nano-molecules in those particles. This study would be the baseline for developing ginger PDENs as a functional food with nano-ingredients, or as an effective vehicle of nano-drug, for human health.
Fast Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Low Concentration of Seminyak (Champeria sp.) Leaf Extract Muhammad Bagas Ananda; Fathan Aditya Sanjaya; Tami Bachrurozy; Helmi Majid Ar Rasyid; Anggraini Barlian; Akfiny Hasdi Aimon; Fitriyatul Qulub; Prihartini Widiyanti; Arie Wibowo
Indonesian Journal of Chemistry Vol 23, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.78545

Abstract

Silver nanoparticles (AgNPs) are fascinating materials for biomedical applications thanks to their strong antibacterial activity and biocompatibility. This study applied the green synthesis method using 0.5 wt.% Seminyak leaf extract and assisted with one min microwave irradiation to enhance AgNPs formation. Extremely small sizes AgNPs with an average particle size of 9.1 ± 4.1 nm and spherical shapes were obtained. The synthesized AgNPs displayed potent antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria with a zone of inhibition of 12.3 ± 0.1 and 13.7 ± 0.7 mm, respectively. The MTT assay results demonstrated that the cells’ viability of the obtained AgNPs was 88.5 ± 7.0 %, implying biocompatibility for biomedical applications.
The Properties of Exosomes Derived from Mesenchymal Stem Cells Preconditioned with L-Ascorbic Acid and Cobalt (II) Chloride Anggraini Barlian; Rizka Musdalifah Amsar; Salindri Prawitasari; Christofora Hanny Wijaya; Ika Dewi Ana; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1100-1110

Abstract

Extracellular vesicles including exosomes, are produced by cells for intracellular communication. Preconditioning of parental cells influences exosome properties. The purpose of this study was to examine the effects of L-ascorbic acid (LAA) and cobalt (II) chloride (CoCl2) on human Wharton’s jelly mesenchymal stem cell (hWJ-MSC)-derived exosomes and their ability to promote stem cell differentiation into chondrocytes. The cells were isolated from the umbilical cord and characterized according to the criteria for mesenchymal stem cell. The cells were cultured in a serum-free medium containing LAA and CoCl2. Cell-produced exosomes were isolated and characterized. hWJ-MSCs can grow in serum-free medium containing LAA and CoCl2. Exosomes derived from hWJ-MSCs had a round morphology, particle size within the exosome range, CD 63 expression, and the capacity to be internalized by cells. The production of exosomes by hWJ-MSCs was enhanced by LAA treatment. LAA and CoCl2 promoted stem cell differentiation into chondrocytes, as indicated by the production of collagen type II and glycosaminoglycans. LAA and CoCl2 affect the properties of MSC-derived exosomes. LAA induces cells to produce exosomes in greater quantities, which have the potential to promote chondrogenic differentiation of stem cells.
Peran Ekstrak Kulit Batang Leea angulata Pada Tahap Proliferasi dalam Proses Penyembuhan Luka Kulit Mencit (Mus musculus): Role of Leea angulata Bark Extract on Proliferation Phase of Wound Healing Process in Mouse Skin (Mus musculus) Hidayah, Thauhidayatul; Barlian, Anggraini
Jurnal Sumberdaya Hayati Vol. 7 No. 2 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.2.71-77

Abstract

Leea angulata is a commonly plant used by the Sasak people as a medicine to heal the wounds, but the effects of L. angulata on wound healing process has not been studied. The aim of this research are to determine the effects of L. angulata bark extracts on wound healing of mouse skin and its influence on the localization of FGF2. 1% of L. angulata bark extracts (P1%) on day 7 can accelerate wound closure amounted at 94.66%, Deep and Superficial Contraction Index of 0.96 and 0.1, epidermis Index and remodeling index of 1.55 and 0.81. L. angulata bark extracts stimulates the synthesis of FGF2 early (3rd day ) compared to the postive control group (5th day). FGF2 in P1% group wa found in epithelial cells in the hair follicles, fibroblasts cell in granulation tissue and epithelial cells in the epidermis. In the positive control group FGF2 was found in in epithelial cell of hair follicle, while in the negative control group, FGF2 undetectable. FGF2 in P1% group detected earlier than the positive control, showing the process of proliferation in P1% accelerated. The results confirm the L. angulata bark extracts accelerate the wound healing in proliferation phase and influence localization of FGF2 in the wound area. Keywords : Leea angulata, wound healing, FGF2, immunohistochemistry
Co-Authors Ahmad Ridwan Akfiny Hasdi Aimon Arie Wibowo Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Diah Ratnadewi Dian Anggraeny Ekavianty Prajatelistia Ergha Widya Sarjana Fathan Aditya Sanjaya Fitria D. Ayuningtyas Fitriyatul Qulub Hari Basuki Notobroto Helmi Majid Ar Rasyid Hermawan Judawisastra Hermawan Judawisastra Hidayah, Thauhidayatul Hutomo Tanoto Iis Rosliana Iis Rosliana Iis Rosliana Ika Dewi Ana Ika Dewi Ana Imam Rosadi Indra Wibowo Indra Wibowo Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Komang A. Wahyuningsih Komang A. Wahyuningsih Kusnandar Anggadiredja Marselina Tan Muhammad Bagas Ananda Ni Luh Wisma Eka Yanti Noviana Vanawati Noviana Vanawati Prihartini Widiyanti Raden Tanita Astrian Ramadhani Eka Putra Regina Giovanni Rini Agustin Rizka Musdalifah Amsar Rizka Musdalifah Amsar Safira Meidina Nursatya Salindri Prawitasari Siti Sobariah Siti Sobariah Siti Sobariah Soraya Rahmanisa Tami Bachrurozy Tias Widyastuti Tias Widyastuti Tias Widyastuti Triati Dewi Kencana Wungu Untung Ari Wibowo Yemima D Riani Yemima Dani Riani Zuliyanto Zakaria