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All Journal HAYATI Journal of Biosciences Jurnal Biologi Udayana AL KAUNIYAH Jurnal Matematika & Sains Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Chemistry Medika Kartika : Jurnal Kedokteran dan Kesehatan 3BIO: Journal of Biological Science, Technology and Management Berkala Penelitian Hayati
Anggraini Barlian
Biology Study Program, School Of Life Sciences And Technology Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Pengaruh Fotoperiode terhadap Respon Stres dan Parameter Reproduksi pada Mencit Jantan (Mus musculus L.) Galur Swiss Webster Ahmad Ridwan; Zuliyanto Zakaria; Anggraini Barlian
Jurnal Matematika & Sains Vol 17, No 1 (2012)
Publisher : Institut Teknologi Bandung

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Abstract

Modernisasi mengubah pola aktivitas manusia dalam rentang waktu 24 jam. Dewasa ini banyak pola aktivitas manusia yang bergeser ke malam hari sehingga terjadi  peningkatan aktivitas dan paparan cahaya hingga malam hari. Pergeseran pola aktivitas ini diduga akan mengubah ritme jam biologis dan dapat menimbulkan stres serta gangguan vitalitas seseorang. Namun kajian tentang pengaruh perubahan fotoperiode terhadap respon stres serta parameter reproduksi masih jarang dilakukan. Penelitian ini menggunakan hewan model mencit SW jantan berumur 4 minggu sebanyak 25 ekor yang dibagi ke dalam 4 kelompok perlakuan berdasarkan durasi fotoperiode yang digunakan yakni 0T, 6T, 18T, 24T dengan 12T sebagai kelompok kontrol (T = durasi terang dalam jam). Intensitas cahaya yang digunakan dalam penelitian ini adalah sebesar 100-110 lux. Seluruh hewan uji didedahkan dengan masing-masing perlakuan fotoperiode selama 2 bulan. Parameter yang diukur meliputi kadar kortikosteron plasma dan berat badan sebagai parameter respon stres, serta kadar testosteron plasma, jumlah sperma dan berat testis sebagai parameter reproduksi. Pengukuran parameter berat badan dilakukan selama pendedahan, sedangkan pengukuran parameter lainnya dilakukan setelah pendedahan fotoperiode. Hasil pengukuran menunjukkan bahwa fotoperiode 0T dan 24T dapat meningkatkan kadar kortikosteron plasma secara signifikan dari kelompok kontrol (p
Pengukuran Efek Antidiabetes Polifenol (Polyphenon 60 ) Berdasarkan Kadar Glukosa Darah dan Histologi Pankreas Mencit (Mus musculus L.) S.W. Jantan yang Dikondisikan Diabetes Mellitus Ahmad Ridwan; Raden Tanita Astrian; Anggraini Barlian
Jurnal Matematika & Sains Vol 17, No 2 (2012)
Publisher : Institut Teknologi Bandung

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Abstract

Diabetes mellitus (DM) adalah penyakit kronis yang ditandai dengan kadar glukosa darah (KGD) yang tinggi (hiperglikemia) akibat pengaturan homeostasis glukosa tidak berjalan sempurna. Penelitian saat ini menunjukkan bahwa stres oksidatif ikut berperan dalam berkembangnya DM. Makanan yang mengandung polifenol cukup menarik perhatian karena peran polifenol sebagai antioksidan dan mampu mengikat radikal bebas sehingga diduga dapat mengurangi resiko terjadinya penyakit DM kronis. Oleh karena itu, penelitian ini dilakukan untuk mengetahui bagaimana pengaruh antioksidan polifenol terhadap mencit yang dikondisikan DM. Hasil penelitian menunjukkan bahwa kelompok mencit DM yang diberi polifenol (kelompok A, B, C) memiliki KGD yang lebih rendah secara signifikan dibandingkan kelompok O (Kontrol DM) dan memiliki toleransi glukosa oral yang lebih baik dibandingkan kelompok O (Kontrol DM). Analisis imunohistokimia menunjukkan bahwa  pulau Langerhans pada kelompok mencit yang diberi polifenol (A, B, C) dan kelompok normal (K) memiliki diameter >100µm, sebaliknya pada kelompok O (Kontrol DM) memiliki diameter 100µm, although group O (DM group) have diameter
Biocompability of silk fibroin scaffold of Bombyx mori with pore of size 100µm towards adipose-derived stem cells (ADSCs) cultured on various of growth medium Imam Rosadi; Karina Karina; Komang A. Wahyuningsih; Anggraini Barlian; Iis Rosliana; Tias Widyastuti; Siti Sobariah; Irsyah Afini
Jurnal Biologi Udayana Vol 24 No 1 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.399 KB) | DOI: 10.24843/JBIOUNUD.2020.v24.i01.p02

Abstract

Rekayasa jaringan terdiri dari 3 komponen utama yaitu sel, nutrisi, dan scaffold. Penggunaan sel punca asal jaringan lemak (adipose-derived stem cells/ ADSCs) telah banyak dikembangkan sebagai sumber sel dalam teknologi rekayasa jaringan. Medium yang digunakan dalam mendukung pertumbuhan sel diantaranya medium yang mengandung serum seperti fetal bovine serum (FBS), kombinasi FBS dan L-ascorbic acid 2-phosphate (LAA) atau platelet-rich plasma (PRP). Pada penelitian ini, sutera asal Bombyx mori diproduksi menjadi scaffold sutera ukuran pori 100µm kemudian ADSCs dikultur diatas scaffold dalam medium mengandung 10% FBS, 10% FBS-LAA atau 10% PRP. Uji yang dilakukan adalah uji pertumbuhan ADSCs yang dikultur pada polystyrene kemudian uji biokompabilitas scaffold sutera pada ADSCs dalam medium mengandung 10% FBS, 10% FBS-LAA dan 10% PRP. Hasilnya menunjukkan bahwa ketiga kelompok ADSCs dalam variasi medium yang mengandung FBS, FBS-LAA atau PRP dapat mendukung pertumbuhan sel. Ketiga medium tersebut juga tidak berbeda bermakna antar kelompok pada uji biokompabilitas ADSCs yang dikultur pada scaffold sutera. Berdasarkan hasil tersebut, scaffold sutera berpotensi sebagai substrat ADSCs yang dapat dikembangkan sebagai teknologi rekayasa jaringan.
KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA Imam Rosadi; Karina Karina; Komang A. Wahyuningsih; Iis Rosliana; Tias Widyastuti; Siti Sobariah; Irsyah Afini; Anggraini Barlian
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2666.248 KB) | DOI: 10.15408/kauniyah.v13i1.12053

Abstract

AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.
Correlation of Hypoxia and Pro-senescence Protein Expression in Green Sea Turtle (Chelonia mydas) Lung Epithelial and Dermal Fibroblast Cell Culture Anggraini Barlian; Yemima Dani Riani
Journal of Mathematical and Fundamental Sciences Vol. 50 No. 1 (2018)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2018.50.1.5

Abstract

Recent studies have shown hypoxia-induced gene expression correlated with cellular senescence. HIF-1α (hypoxia-inducible factor 1-alpha), p53, and pRB were induced under hypoxia and correlated with cellular senescence. The localization and expression of HIF-1α, p53, and pRB in Chelonia mydas lung epithelial and dermal fibroblast cell cultures were analyzed under normoxic and hypoxic conditions (at 4 and 24 hours). Human dermal fibroblast was used for comparison purposes. Protein localization was analyzed with immunocytochemistry, while protein expression was analyzed with the Western blot and enhanced chemiluminescence (ECL) method. HIF-1α, p53, and pRB were localized in the nuclei of the C. mydas cell cultures treated with hypoxia. The C. mydas lung epithelial cell cultures had a higher increase of HIF-1α expression than the human dermal fibroblast cell culture. The hypoxic conditions did not affect p53 expression significantly in C. mydas lung epithelial and dermal fibroblast cell cultures. Meanwhile, pRB expression changed significantly under hypoxia in the C. mydas dermal fibroblast cells. Expression of p53 and pRB in the human cell cultures was higher than in the C. mydas cell cultures. This research suggests that C. mydas and human cell cultures have different pro-senescence protein expression responses under hypoxic conditions.
Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold Regina Giovanni; Untung Ari Wibowo; Hermawan Judawisastra; Anggraini Barlian
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 3 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.3.7

Abstract

Skin tissue engineering is a developing technology to heal severe wounds. Combining polyvinyl alcohol (PVA) and silk fibroin (SF) nanofibers is a promising method of developing a skin scaffold because the resulting structure mimics collagen fibers. The aim of this research was to study the growth of human dermal fibroblasts (HDF) on a polyvinyl alcohol-silk fibroin (PVA-SF) nanofiber scaffold that was produced by electrospinning. Morphological characterization and chemical analysis of the scaffold were performed by scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR), and contact angle measurement. The biocompatibility of the scaffold was tested by MTT cytotoxicity assay, SEM analysis, adherence ratio calculation, and analysis of the HDF growth curve for 9 days. The FTIR results confirmed the presence of SF and PVA. The average fiber diameter and pore size of the PVA scaffold were greater than those of the PVA-SF scaffold. Both scaffolds had hydrophilic properties and were not cytotoxic. Thus, HDF can attach and grow on both types of scaffold better than HDF seeded on a polystyrene plate. In conclusion, the addition of SF to the PVA nanofibers caused bead formation, which affected the substrate topography, decreased hydrophilicity and also decreased the fiber diameter and pore size in the nanofiber scaffold compared to the PVA nanofiber scaffold without SF addition. SF addition increases cell attachment to the nanofiber scaffold and has potential to facilitate HDF cell growth.
Glycosaminoglycans Content and Type II Collagen Localization in Chondrogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Induced by L-Ascorbic Acid 2-Phosphate Anggraini Barlian; Ni Luh Wisma Eka Yanti
Journal of Mathematical and Fundamental Sciences Vol. 52 No. 1 (2020)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2020.52.1.7

Abstract

L-ascorbic acid 2-phosphate (LAA) is known to induce chondrocyte differentiation. The objective of this study was to analyze the potency of LAA in chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSC) by analyzing the glycosaminoglycans (GAG) content and type II collagen (Coll2) localization. ADSC was characterized using flow cytometry and cultured in media containing various concentrations of LAA (0, 25, 50, 100 μg/mL) for 2, 3 and 4 weeks. Coll2 localization was analyzed by immunocytochemistry (ICC) using a confocal microscope. The quantification of GAG was performed by Alcian Blue staining and calcium deposition by Alizarin Red S staining. The results showed that ADSC was positive for mesenchymal stem cell (MSC) markers. Coll2 was localized in the cytoplasm and showed increasing abundance along with the increase of the LAA concentration. The highest intensity of Coll2 localization was shown in LAA 100 μg/mL. ADSC in LAA induction medium showed higher GAG content compared to the control group (LAA 0 μg/mL) (p < 0.05). The highest calcium deposit was shown by LAA 25 μg/mL after 4 weeks of culture (p < 0.05) and it decreased at higher concentrations. In conclusion, LAA 100 μg/mL is considered the optimum LAA concentration for chondrogenic differentiation.
Role of Hypoxia on Growth and Differentiation of Human Adipose Derived Stem Cells Grown on Silk Fibroin Scaffold Induced by Platelet Rich Plasma Anggraini Barlian; Marselina Irasonia Tan; Ergha Widya Sarjana; Noviana Vanawati
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 3 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.3.6

Abstract

Previous research has proven that 10% platelet-rich plasma (PRP) can enhance growth and differentiation of human adipose derived stem cells (hADSC) grown on silk fibroin scaffold into chondrocytes. A low oxygen concentration (hypoxia) condition is an important factor that potentially affects the ability of hADSC to grow and differentiate. The objective of this research was to analyze the difference in growth and differentiation capacity of hADSC grown on salt leached silk fibroin scaffold supplemented by 10% PRP under normoxic and hypoxic conditions. The growth capacity of the hADSC was determined by MTT assay and differentiation was tested using glycosaminoglycan (GAG) content analysis, while chondrocyte markers were visualized with the immunocytochemistry (ICC) method. This research observed hADSC proliferation under normoxic and hypoxic conditions for 21 days. Visualization of type 2 collagen showed that it was more abundant under hypoxia compared to normoxia.  HIF-1α was only detected in the hADSC cultured in hypoxic conditions. In conclusion, culture under hypoxic conditions increases the capacity of hADSC to grow and differentiate into chondrocytes. This is the first study that has shown that hypoxia is able to enhance the proliferation and differentiation of hADSC grown on 3D salt leached silk fibroin scaffold supplemented by 10% PRP.
Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering Safira Meidina Nursatya; Anggraini Barlian; Hermawan Judawisastra; Indra Wibowo; Hutomo Tanoto
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 2 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.2.10

Abstract

This study aimed to determine the characteristics of scaffolds made of fibroin from Bombyx mori and spidroin from Argiope appensa in supporting the attachment and proliferation of HDF cells on the scaffolds. Thin-film scaffolds were made using the solvent casting technique, where the scaffold is an amalgamation of fibroin, spidroin, PVA, and glycerol. HDF cells were grown on DMEM medium with 10% FBS and 1% antibiotic-antimicotic. Characterization of the scaffolds was performed by using ATR-FTIR, swelling test, contact angle measurement, tensile test, biodegradation, MTT and SEM. The results of the ATR-FTIR analysis showed that the scaffolds contained fibroin, spidroin, PVA, and glycerol. Swelling and contact angle tests showed that all scaffold combinations were hydrophilic. Mechanical properties and in vitro biodegradation tests showed no significant difference among the scaffold combinations. MTT testing showed that all scaffolds could facilitate the attachment of fibroblasts and showed increased viability from day 1, 3, and 5. Scanning electron microscopy showed that the cells in the 70% fibroin and 10% spidroin scaffold had the best cell morphology and the best combination for potential application in skin tissue engineering.
THE PATTERNS OF SEX DETERMINATION AND DIFFERENTIATION GENES IN GREEN SEA TURTLE (Chelonia mydas) Anggraini Barlian; Noviana Vanawati; Fitria D. Ayuningtyas
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.808 KB) | DOI: 10.23869/104

Abstract

Green sea turtle (C. mydas) is one of TSD (Temperature-dependent Sex Determination, TSD) animals which mean that their sex is determined by the egg’s incubation temperature. Genotypic Sex Determination (GSD) homologous genes play a role in TSD process. Until now, research on the pattern of sex determination genes in C.mydas has not been conducted yet. The aim of this research is to reveal sex determination and differentiation genes expression in Mesonephros-Gonad (MG) complexes of C. mydas embryos which incubated in masculinizing temperature (MT) and feminizing temperature (FT). C. mydas eggs were incubated in 3 different stage of TSP (Thermosensitive Period) at masculinizing temperature (26±10C, MT) and feminizing temperature (31±10C FT). Mesonefros-gonad complexes were isolated at Pre-TSP stage (FT at 14th day, MT at 24th day), TSP stage (FT at 24th day, MT at 36th day) and differentiated stage (FT at 40th day, MT at 58th day). RNA from mesonephros-gonad (MG) complexes were converted into cDNA by RT-PCR process. Pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9 and Rspo1 genes expression were analyzed by quantitative Real Time PCR (qPCR) method with β-actin gene as an internal control. Result of this study shown that expression pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9, and Rspo1genes in gonadal embryo of C. mydas were increased during gonadal development stage. Four genes expression patterns (Wnt4, Fgf9, Rspo1, and FoxL2) have shown that these genes have role in sexual differentiation rather than in sexual determination.
Co-Authors Ahmad Ridwan Akfiny Hasdi Aimon Arie Wibowo Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Diah Ratnadewi Dian Anggraeny Ekavianty Prajatelistia Ergha Widya Sarjana Fathan Aditya Sanjaya Fitria D. Ayuningtyas Fitriyatul Qulub Hari Basuki Notobroto Helmi Majid Ar Rasyid Hermawan Judawisastra Hermawan Judawisastra Hidayah, Thauhidayatul Hutomo Tanoto Iis Rosliana Iis Rosliana Iis Rosliana Ika Dewi Ana Ika Dewi Ana Imam Rosadi Indra Wibowo Indra Wibowo Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Komang A. Wahyuningsih Komang A. Wahyuningsih Kusnandar Anggadiredja Marselina Tan Muhammad Bagas Ananda Ni Luh Wisma Eka Yanti Noviana Vanawati Noviana Vanawati Prihartini Widiyanti Raden Tanita Astrian Ramadhani Eka Putra Regina Giovanni Rini Agustin Rizka Musdalifah Amsar Rizka Musdalifah Amsar Safira Meidina Nursatya Salindri Prawitasari Siti Sobariah Siti Sobariah Siti Sobariah Soraya Rahmanisa Tami Bachrurozy Tias Widyastuti Tias Widyastuti Tias Widyastuti Triati Dewi Kencana Wungu Untung Ari Wibowo Yemima D Riani Yemima Dani Riani Zuliyanto Zakaria