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All Journal Jurnal Ilmu Pertanian Indonesia Journal of Tropical Soils Biotropika Planta Tropika BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Biosaintifika: Journal of Biology & Biology Education Biosaintifika: Journal of Biology & Biology Education Berita Biologi
Sarjiya Antonius
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Cibinong
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol 29, No 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Abstract

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.
The Effect of Arbuscular Mycorrhiza-Enriched Biocompost and Organic Fertilizer in Reducing Heavy Metal Absorption in Shallots Akib, Muhammad Akhsan; Antonius, Sarjiya; Kuswinanti, Tutik; Syatrawati, Syatrawati; Dewi, Tirta Kumala; Sutisna, Entis
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.21684

Abstract

Shallots have physiological and pharmacological effects on the human body and have been cultivated intensively using biocompost, which may be contaminated with heavy metals. Enrichment of arbuscular mycorrhizal (AM) fungi in biocompost and the addition of biological organic fertilizer (BOF) to reduce the absorption of heavy metals in shallot bulbs is the main objective of this research. The study used a Randomized Block Design with five treatments, namely: shallots cultivation method by indigenous farmers/control(B0); Dose of biocompost enriched with AM fungi 100kg plot-1(B1); 200kg plot-1(B2); 100kg plot-1+BOF(B3); 200kg plot-1+BOF(B4), has been carried out in Enrekang district, South Sulawesi by observing the components of heavy metal content in plant organs and the level of infection of AM fungi in plant roots. Results showed that a dose of biocompost enriched with AM fungi 200 kg plot-1, and 100kg plot-1+BOF could increase Cr, Cu, and Pb accumulation in roots and leaf, as well as reduce metal accumulation in shallot bulbs, with level AM fungi infection classified as very high. The novelty of this study is that organic fertilizer enriched with AM fungi can reduce the absorption of heavy metals that accumulate in shallot bulbs and is essential information for farmers.
Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol. 29 No. 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Abstract

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.
Isolation and Characterization of Propoxur-Degrading Bacteria, Brucella pseudintermedia LED 6 from a Pineapple Plantation in Lampung Hidayat, Taufiq; Widyastuti, Rahayu; Djajakirana, Gunawan; Antonius, Sarjiya; Dewi, Tirta Kumala
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.725

Abstract

Propoxur is a non-systemic carbamate insecticide that is widely used in Indonesia to control insect pests. However, its persistence and toxicity pose environmental concerns. Bioremediation with bacteria is a viable method for mitigating the detrimental effects of propoxur residues. The goal of this work was to isolate and characterize bacterial strains that can degrade propoxur. Isolation by enrichment culture procedures, utilizing propoxur as the sole carbon source. The isolates' morphological and physiological features were examined, and their degradation potential was determined. Six bacterial samples were isolated from a pineapple plantation in Lampung, Indonesia, and one strain, known as LED 6, showed great potential for propoxur breakdown. Molecular identification with 16S rRNA gene sequencing identified the isolate as Brucella pseudintermedia. Growth characterisation revealed that the isolate performed best at 28 °C and pH 7. After 72 hours of incubation with 500 ppm propoxur, LED 6 had deteriorated around 26% of the starting concentration. Keywords: bioremediation, Brucella pseudintermedia, pineapple, propoxur
Selection of Potential Lignin-Degrading Bacteria and Fungi Isolates from the Gunung Lumut and Sungai Wain in East Kalimantan Ruhimat, Riki; Kumala Dewi, Tirta; Noviana, Zahra; Sutisna, Entis; Mulyani, Nani; Juwari, Micha; Sufaati, Supeni; Antonius, Sarjiya; Verchius, Eric
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.14755

Abstract

The enzymes laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) are ligninolytic enzyme groups that have a crucial role in degrading lignin compounds. These complex components are abundant in plant biomass. This research aims to isolate and characterize microorganisms producing the enzymes Lac, MnP, and LiP from leaf litter, rhizosphere soil, and sediment in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan. The bacterial and fungal isolates obtained were cultured on NA and PDA media enriched with 0.05% guaiacol as a substrate. Initial selection was carried out based on the colony growth rate and the formation of a brownish discoloration zone, which indicates ligninolytic enzyme activity. The superior isolates were then tested for extracellular enzyme activity quantitatively using a UV-Vis spectrophotometer. Among the bacterial isolates, the highest Lac activity is isolated GLS 5.1.2 (173.44 U L-1); the highest MnP activity is isolated GLS 5.3 (177.41 U L-1); and the highest LiP activity is isolated GLS 5.1.2 (1034.05 U L-1). In the group of fungi, isolate SS2 was detected with the highest Lac activity (20.66 U L-1), MnP activity (333.75 U L-1), and LiP activity (2516.13 U L-1). Biodiversity sources in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan have the potential to produce microbial isolates with unique enzymatic characteristics that have never been reported before. The resulting microbial isolate can be used as a bioactivator in composting organic waste to produce high-quality organic fertilizer.
POTENSI RHIZOBAKTERI DALAM MENDUKUNG PERTUMBUHAN STEK BATANG SINGKONG (Manihot esculenta Crantz) [The Potential of Rhizobacteria in Supporting the Growth of Cassava (Manihot esculenta Crantz ) Stem Cuttings] Nugroho, Agung Adi; Agustiyani, Dwi; Sutisna, Entis; Mulyani, Nani; Dewi, Tirta Kumala; Nditasari, Achirul; Antonius, Sarjiya; Purwaningsih, Sri
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.120

Abstract

Cassava (Manihot esculenta Crantz) is an important food plant in Indonesia, particularly as a carbohydrate source. We require a plan to increase production and quality. The use of Plant Growth Promoting Rhizobacteria (PGPR) in organic fertilizers is one option. The aim of this study is to isolate, characterize, and evaluate potential microbial candidates for cassava greenhouse growth. K solubilizing, P solubilizing, N fixing, IAA production, protease production, ACC-deaminase, and siderophore production were investigated in 15 tuber root rhizobacteria isolates. The bacterial isolates' ability to grow cassava stem cuttings was then assessed. The rhizobacterial inoculation treatments in this study included fifteen single isolates, a combination of isolates, and controls. With three replications, the experimental design was completely randomized. Five P-solubilizing bacterial isolates (PK AL.2.2, PK LAS 4A.4, PK 4.1, PK Al.2.6, PK Kbm 6.1), two protease-producing isolates (Pro LAS 4B.6 and Pro LAS 5A.3), and one IAA-producing isolate (TSB LAS 1A.6) increased wet plant weight by 20-41% as compared to controls. The results of molecular identification of the six excellent isolates were Burkholderia sp. (PK AL.2.2), Paenarthrobacter nicotinovorans (Pro LAS 4B.6), Burkholderia territorii (PK 4.1), Burkholderia cenocepacia (Pro LAS 5A.3), Burkholderia territorii (PK LAS 4A.4), and Kocuria rhizophila (TSB LAS 1A.6).
Co-Authors Achirul Nditasari Agung Adi Nugroho, Agung Adi Akib, Muhammad Akhsan Andi Salamah Dewi, Tirta Kumala DEWI, TIRTA KUMALA Dori Kusuma Jaya Dwi Agustiyani Entis Sutisna Gunawan Djajakirana Jamhari Jamhari Juwari, Micha Kumala Dewi, Tirta Lekatompessy, Sylvia J. R. Maman Rahmansyah Mulyani, Nani Muslichah, Dwi Agustiyani Novik Nurhidayat Nur Laili RAHAYU WIDYASTUTI Ruhimat, Riki Rumella Simarmata, Rumella Sahputra, Rozy Dwi Sahputra, Rozy Dwi Solly Aryza Sri Purwaningsih Sukmadewi, Desak Ketut Tristiana Supeni Sufaati Syatrawati Taufiq Hidayat Tiwit Widowati, Tiwit Tutik Kuswinanti Verchius, Eric Yulia Nuraini Zahra Noviana