Garuda - Garba Rujukan Digital

Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol 29, No 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.

The Effect of Arbuscular Mycorrhiza-Enriched Biocompost and Organic Fertilizer in Reducing Heavy Metal Absorption in Shallots Akib, Muhammad Akhsan; Antonius, Sarjiya; Kuswinanti, Tutik; Syatrawati, Syatrawati; Dewi, Tirta Kumala; Sutisna, Entis
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.21684

Shallots have physiological and pharmacological effects on the human body and have been cultivated intensively using biocompost, which may be contaminated with heavy metals. Enrichment of arbuscular mycorrhizal (AM) fungi in biocompost and the addition of biological organic fertilizer (BOF) to reduce the absorption of heavy metals in shallot bulbs is the main objective of this research. The study used a Randomized Block Design with five treatments, namely: shallots cultivation method by indigenous farmers/control(B0); Dose of biocompost enriched with AM fungi 100kg plot-1(B1); 200kg plot-1(B2); 100kg plot-1+BOF(B3); 200kg plot-1+BOF(B4), has been carried out in Enrekang district, South Sulawesi by observing the components of heavy metal content in plant organs and the level of infection of AM fungi in plant roots. Results showed that a dose of biocompost enriched with AM fungi 200 kg plot-1, and 100kg plot-1+BOF could increase Cr, Cu, and Pb accumulation in roots and leaf, as well as reduce metal accumulation in shallot bulbs, with level AM fungi infection classified as very high. The novelty of this study is that organic fertilizer enriched with AM fungi can reduce the absorption of heavy metals that accumulate in shallot bulbs and is essential information for farmers.

Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia Jaya, Dori Kusuma; Giyanto, Giyanto; Nurhidayat, Novik; Antonius, Sarjiya
JOURNAL OF TROPICAL SOILS Vol. 29 No. 3: September 2024
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2024.v29i3.149-157

Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool. One such gene is the rhizobacterial acdS, which codes for synthesizing the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful conditions. This study aims to investigate the abundance of the acdS gene in different abiotic and biotic stressors of the rhizospheric soil of pineapple plantation area that has been exposed incidentally to herbicides, flooding, and pathogenic Phytophthora spp infections. Soil samples were collected by using simple randomized sampling. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess the gene abundance. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated ones. The highest abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in abundance between the fflooded rhizospheric soil TO rhizospheric soil of flooded-exposed plants and the non-exposed ones. Hopefully, this data will help us better understand proper land-use management practices for pineapple plants.

Isolation and Characterization of Propoxur-Degrading Bacteria, Brucella pseudintermedia LED 6 from a Pineapple Plantation in Lampung Hidayat, Taufiq; Widyastuti, Rahayu; Djajakirana, Gunawan; Antonius, Sarjiya; Dewi, Tirta Kumala
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.725

Propoxur is a non-systemic carbamate insecticide that is widely used in Indonesia to control insect pests. However, its persistence and toxicity pose environmental concerns. Bioremediation with bacteria is a viable method for mitigating the detrimental effects of propoxur residues. The goal of this work was to isolate and characterize bacterial strains that can degrade propoxur. Isolation by enrichment culture procedures, utilizing propoxur as the sole carbon source. The isolates' morphological and physiological features were examined, and their degradation potential was determined. Six bacterial samples were isolated from a pineapple plantation in Lampung, Indonesia, and one strain, known as LED 6, showed great potential for propoxur breakdown. Molecular identification with 16S rRNA gene sequencing identified the isolate as Brucella pseudintermedia. Growth characterisation revealed that the isolate performed best at 28 °C and pH 7. After 72 hours of incubation with 500 ppm propoxur, LED 6 had deteriorated around 26% of the starting concentration. Keywords: bioremediation, Brucella pseudintermedia, pineapple, propoxur

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search