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Uji Potensi Perlekatan Bakteri Asam Laktat Isolat TLA-15 Dan TLA-20 Pada Sel Epitel Usus Tikus (Rattus norvegicus) Wijayanti, Ernanin Dyah; Ardyati, Tri
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 1, No 2 (2011): Oktober 2010 - Maret 2011
Publisher : University of Muhammadiyah Malang

In Vitro probiotic potential test that had been conducted before, which consist of probiotic bacteria testÂ resistance toward the gastro-intestinal track pH and the bile salt concentration shows that the strain bacteria TLA-15 and TLA-20 had potential as probiotic bacteria. An advance test are required to fullfill the requirement as the probiotic bacteria. So that, the objective of this research are to study the viability of lactic acid bacteria strain TLA-15 and TLA-20 at the Rattus norvegicus GI track. At the first step of this research was added 4 106 cell/ml lactic acid bacteria TLA-15 and TLA-20 given orally.

PRODUKSI SELULOSA BAKTERIAL DARI AIR BUAH KELAPA DALAM BERBAGAI KONSENTRASI SUKROSA DAN UREA (PRODUCTION OF BACTERIAL CELLULOSE FROM COCONUT FRUIT WATER Ardyati, Tri; Zubaidah, Elok; Munawaroh, Munawaroh; P, Citra Pradani; Suharjono, Suharjono
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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ABSTRACT Cellulose is a nature biopolymer that mainly derived from plant and it has been application broadly in textile and paper industries. Usage of forest plants to cellulose fiber production continually caused negative impact to environment. Waste of coconut fruit water can be metabolism by some species of Gluconacetobacter (Acetobacter) to produce bacterial cellulose as alternative of plant cellulose. The objective of the research is to study effect of increasing of sucrose and urea concentration to bacterial cellulose productivity in coconut fruit water medium. Starter of microbial culture 10% with 2.2 x 107 cell/mL (90% of bacteria and 10 % of yeast) was inoculated into coconut fruit water medium with variation of sucrose and urea concentration. It was incubated seven days in static culture at room temperature. Productivity of cellulose bacterial highest was 10.849 gram in the medium with 5.0 % sucrose and 0.25 % urea concentration. Key words: Acetobacter, cellulose, sucrose, urea ABSTRAK Selulosa adalah biopolimer alamiah yang sebagian besar diperoleh dari tanaman dan telah diaplikasikan secara luas terutama di industri kertas dan tekstil. Penggunaan tanaman hutan untuk produksi serat selulosa secara kontinyu mengakibatkan dampak negatif pada lingkungan. Limbah air buah kelapa dapat dimetabolisme oleh bakteri anggota Genus Gluconacetobacter (Acetobacter) menghasilkan selulosa bakterial sebagai alternatif bagi selulosa tanaman. Tujuan penelitian adalah untuk mempelajari pengaruh peningkatan konsentrasi sukrosa dan urea pada produktivitas selulosa bakterial dalam medium air buah kelapa. Starter suspensi mikrobia 10% dengan densitas 2,2 x 107 sel/ml (90% bakteri dan 10 % khamir) diinokulasikan ke medium air buah kelapa 150 mL dengan variasi konsentrasi sukrosa dan urea yang dibiakkan secara statis selama tujuh hari pada suhu ruang. Produktivitas selulosa tertinggi 10,849 gram pada formula medium dengan konsentrasi sukrosa 5 % dan urea 0,25 %. Kata kunci: Acetobacter, selulosa, sukrosa, urea

PRODUKSI SELULOSA BAKTERIAL DARI AIR BUAH KELAPA DALAM BERBAGAI KONSENTRASI SUKROSA DAN UREA (Production Of Bacterial Cellulose From Coconut Fruit Water Suharjono, Suharjono; Ardyati, Tri; Zubaidah, Elok; Munawaroh, Munawaroh; P, Citra Pradani
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 8, No 1 (2011): Prosiding Seminar Nasional VIII Biologi
Publisher : Universitas Sebelas Maret

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ABSTRACT Cellulose is a nature biopolymer that mainly derived from plant and it has been application broadly in textile and paper industries. Usage of forest plants to cellulose fiber production continually caused negative impact to environment. Waste of coconut fruit water can be metabolism by some species of Gluconacetobacter (Acetobacter) to produce bacterial cellulose as alternative of plant cellulose. The objective of the research is to study effect of increasing of sucrose and urea concentration to bacterial cellulose productivity in coconut fruit water medium. Starter of microbial culture 10% with 2.2 x 107 cell/mL (90% of bacteria and 10 % of yeast) was inoculated into coconut fruit water medium with variation of sucrose and urea concentration. It was incubated seven days in static culture at room temperature. Productivity of cellulose bacterial highest was 10.849 gram in the medium with 5.0 % sucrose and 0.25 % urea concentration. Key words: Acetobacter, cellulose, sucrose, urea ABSTRAK Selulosa adalah biopolimer alamiah yang sebagian besar diperoleh dari tanaman dan telah diaplikasikan secara luas terutama di industri kertas dan tekstil. Penggunaan tanaman hutan untuk produksi serat selulosa secara kontinyu mengakibatkan dampak negatif pada lingkungan. Limbah air buah kelapa dapat dimetabolisme oleh bakteri anggota Genus Gluconacetobacter (Acetobacter) menghasilkan selulosa bakterial sebagai alternatif bagi selulosa tanaman. Tujuan penelitian adalah untuk mempelajari pengaruh peningkatan konsentrasi sukrosa dan urea pada produktivitas selulosa bakterial dalam medium air buah kelapa. Starter suspensi mikrobia 10% dengan densitas 2,2 x 107 sel/ml (90% bakteri dan 10 % khamir) diinokulasikan ke medium air buah kelapa 150 mL dengan variasi konsentrasi sukrosa dan urea yang dibiakkan secara statis selama tujuh hari pada suhu ruang. Produktivitas selulosa tertinggi 10,849 gram pada formula medium dengan konsentrasi sukrosa 5 % dan urea 0,25 %. Kata kunci: Acetobacter, selulosa, sukrosa, urea

KARAKTERISTIK NUTRISI SUSU KERBAU BELANG TORAJA, MAKASSAR Alang, Hasria; Kusnadi, Joni; Ardyati, Tri; Suharjono, Suharjono
ZOOTEC Vol 40, No 1 (2020)
Publisher : Universitas Sam Ratulangi

NUTRITION CHARACTERISTICS OF BUFFALO MILK FROM TORAJA, MAKASSAR. The study was conducted to analyze the nutritional content of milk in Toraja Belang Buffalo. The research method took samples of Toraja Belang buffalo milk in Toraja Regency, South Sulawesi. Sample was put at the cool box before analyzing its nutritional content. The nutrient content of the milk sample was analyzed using a Lacto scan analyzer to see water content, ash content, protein, fat, lactose and BKTL. The results showed that nutritional content of Belang Toraja buffalo milk consisted of protein (3.17%), fat (12.97%), lactose (4.37%), water content (79.49%), ash content (0.88 %) and SNF / BKTL (8.42%).

Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) and Optimization of Cellulase Activity as Animal Feed: Screening of Cellulolytic Bacteria from Sugarcane Waste (Bagasse) Ramadhani, Sulistya Ika; Ardyati, Tri; Sjofjan, Osfar
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.19

The Sugar Factory of Tjoekir Jombang is one of the sugar factories located in Jombang, East Java under PT Perkebunan Nusantara X. Sugarcane waste, also known as bagasse causes pollution of the environment. Some microorganisms are able to degrade cellulose-containing bagasse, because of cellulolytic enzymes produced by cellulolytic bacteria. This study aims to obtain cellulolytic bacteria isolates, screen the highest cellulolytic activity, identify the highest cellulolytic activity isolates, and optimize conditions (pH and temperature) for the highest cellulase activity. Cellulolytic bacteria from bagasse were grown on a medium containing 1% CMC. Several additional minerals were other than those in the CMC medium. The cellulase activity was assayed semi-quantitatively with the addition of 0.1% Congo red and quantitatively using the 3,5-Dinitro Salicylic Acid (DNS) method. Bacterial isolates with high cellulolytic activity were identified based on the 16S rDNA sequence. This research obtained 20 bacterial isolates, where isolate A1T4 had the highest cellulolytic index of 1.18 mm. Measurement of cellulase activity using the DNS method showed that isolate A2T2 had the highest cellulase activity of 2.19 U/mL. Hemolysis assay showed that from 12 isolates, only two isolates have γ-hemolysis activity (isolates A1T6 and A3T3). Those isolates were optimized in a CMC broth medium with temperatures of 30°C and 37°C and pH 5 and pH 6 to produce the highest cellulase activity. Isolate A1T6 and A3T3 were grown optimally at 30oC and pH 6. Isolate A1T6 was identified as Citrobacter amalonaticus with a similarity of 99.80%, and isolate A3T3 was identified as Pseudomonas mendocina with a similarity of 98.83%.

Peningkatan Pemahaman Guru Biologi SMA Kota Malang tentang Evolusi Mikroba melalui Teknologi CRISPR dan Mekanisme Resistensi Antibiotik Jatmiko, Yoga Dwi; Suharjono, Suharjono; Ardyati, Tri; Mustafa, Irfan
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 1 (2025): April
Publisher : Politeknik Negeri Jember

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The implementation of Merdeka Curriculum in high school requires Biology teachers to face new challenges in teaching, especially the topic of microbial evolution. Microbes play an important role as a model in understanding the evolutionary process, including through CRISPR technology (genome editing). In addition, the mechanism of antibiotic resistance is also a clear example of evolution through genetic changes. However, limited access to information and facilities is an obstacle for teachers in developing appropriate learning modules. This service activity aims to improve the insight and competence of Biology teachers related to microbial evolution, through the introduction of CRISPR technology and antibiotic resistance, both in theory and practice. The training began with a pre-training survey on antibiotic use, material delivery and continued with antibiotic sensitivity test practice. Evaluation in the form of pretest and posttest was also given to participants who were categorized on a scale of understanding: very low, low, medium, high, and very high. The training participants had sufficient experience in the use of antibiotics before the training, but their understanding of antibiotics still varied. The pretest results obtained an understanding of 8.3% very low, 20.8% low, 33.3% medium, 33.3% high, and 4.2% very high. The posttest results showed a significant difference, with only two categories, 38.1% high and 61.9% very high. This result shows that the participants' understanding has increased by 28.4% with an average pretest score of 62 and posttest score of 87. In addition, participants were also able to conduct antibiotic sensitivity tests and how to interpret the data. The participants' response was very positive, with a satisfaction index score between 94-97 which is classified as very good. The theory and practice obtained are expected to increase teacher innovation in learning for students to understand microbial evolution.

Isolation and Screening of Lactic Acid Bacteria From Sumbawa Buffalo Milk (Bubalus bubalis) as Potential Starter Cultures Harmoko, Deni; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.03

The Sumbawa buffalo (Bubalus bubalis) is one of the ruminant livestock in Indonesia that not only contributes to fulfilling meat requirements but also milk. Besides containing nutrients that are very beneficial for human health, buffalo milk is also a potential source of lactic acid bacteria (LAB) with technological and functional properties. Lactic acid bacteria have been utilized as starter cultures in various fermented products. This study aimed to isolate LAB from Sumbawa buffalo milk and to identify the potential isolate as a starter culture. The screening of LAB as a starter culture was based on some technological properties, including proteolytic activity, lipolytic activity, exopolysaccharide (EPS) production, antibacterial activity, antibiotic sensitivity, hemolytic activity, and acidification activity Data were analyzed statistically using one-way ANOVA and Tukey’s post hoc test at a 5% significance level. A total of 21 isolates were isolated from fresh buffalo milk, with a LAB total was 4.7x105 CFU.mL-1. All the isolates were characterized as Gram-positive with cocci-shaped. The SA8 isolate was selected as the most potential candidate as a starter culture because it has fulfilled the criteria such as the highest proteolytic activity, the lowest lipolytic activity, producing EPS, potential antagonistic activity against Bacillus cereus, Escherichia coli, and Salmonella Typhi, and sensitivity to cefazolin, intermediate to erythromycin and cinoxacin, non-pathogen, as well as the most rapid acidification activity. The SA8 isolate was identified as Enterococcus lactis with a similarity level of 99.99% towards strain BT159. This indigenous LAB was a potential starter culture of Sumbawa fermented buffalo milk to increase the diversification of products derived from buffalo milk. Keywords: Enterococcus lactis, lactic acid bacteria, technological properties, starter culture, Sumbawa buffalo milk.

Halophilic Bacteria Producing Protease from Salted Fish in Ponrang, Luwu Regency, South Sulawesi Ramadhan, Andi Muhammad Faiz; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.06

The need for protease enzymes for medical and industrial purposes. The need for proteases in the world reaches 65% of the total sales of enzymes, and in Indonesia can reach 2.500 tons every year, and 99% percent of the enzyme needs are still imported from abroad. Salted fish is one of the foods that contain a lot of protein, which is about 42% in 100 g of salted fish. It allows the presence of proteolytic bacteria that have halophilic properties in salted fish. This study aims to explore the presence of proteolytic bacteria with halophilic properties in salted fish. Proteolytic isolates were isolated using SMA media from salted fish from Ponrang District, Luwu Regency, South Sulawesi. A qualitative test was carried out by measuring the clear zone formed in Skim Milk Agar (SMA). From the isolation process, 51 isolates were obtained. However, after the screening, only 25 pure isolates were found that formed a clear zone, followed by a quantitative test to see which isolates had stable activity at incubation times of 24, 48, and 72 hours using Tryptic Soy Broth (TSB) media. The results obtained four superior isolates, P1A1K, P2B2PS, P3C6PS, and P3C6P, then continued to quantify halophilic bacteria properties by administering NaCl with 15% and 20% concentration into TSB media for 24 and 48 hours incubation. Two isolates with the highest protease activity were P3C6P isolates with the activity of 43.23 ± 7.11 U.mL-1 at 15% salt concentration and 42.83 ± 3.04 U.mL-1 at 20% salt concentration and P2B2PS isolates of 38.05 ± 4.05 U.mL-1 at 15% salt concentration and 38.15 ± 1.47 U.mL-1 at 20% salt concentration. The two isolates were then tested for pathogenicity on blood agar media. It was found that only P3C6P isolates did not have pathogenic properties, so P3C6P isolates were continued with catalase, oxidase, and gram staining tests, which showed negative catalase and oxidase results and were gram-positive, followed by identification based on sequences. 16S rDNA and phylogenetic tree construction where isolate P3C6P was identified as Bacillus cereus with a 100% similarity level to the WHX1 strain. Keywords: Enzyme, Protease, Proteolytic, Salted Fish, 16s-rDNA.

Uji Aktivitas Bakteriofage Litik dari Limbah Rumah Tangga Terhadap Salmonella Typhi Jatmiko, Yoga Dwi; Purwanto, Agung Putra; Ardyati, Tri
Jurnal Biodjati Vol 3 No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3471

Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan SalmonellaTyphi. Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap SalmonellaTyphi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae.Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 Â± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 Â± 0,31 log sel/mL) dan S2-St (7,60 Â± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella Â Typhi.Â

Identification of Bacterial Isolates from Mozzarella Cheese Whey Using 16S rDNA and Assessment of Their Consortium Proteolytic Activity: Identification and Protease Activity of Cheese Whey Bacteria Maulidiyah, Nuris; Ardyati, Tri; Dwi Jatmiko, Yoga
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.15

Cheese whey is the main waste from cheese production, which contains high levels of protein, lactose, and minerals. However, if not properly managed, its acidic pH and high organic load can negatively impact the environment when discharged directly. Its protein content makes it a habitat for proteolytic bacteria that are capable of producing protease enzymes to hydrolyze proteins into peptides and amino acids. Moreover, the utilization of proteolytic bacteria from cheese whey offers a biotechnological solution for waste management while opening up opportunities for industrial applications, such as fermentation and the production of functional enzymes. The aim of this study is to analyze the protease activity of single bacterial isolates and bacterial consortia isolated from cheese whey and identify isolates based on 16S rDNA. The first stage of the research was screening pathogenicity based on hemolysis analysis using a blood agar medium. The second stage tested for synergism among isolates. The ability of isolates to produce proteolytic enzymes qualitatively and quantitatively, and then the identification of proteolytic bacterial species based on 16S rDNA. Hemolysis assay of eight isolates resulted in three isolates (A8, C7, and C8) showing gamma hemolysis. The protease activity assay of three isolates was measured at an incubation period of 0-72 hours; the consortium (cn) isolate exhibited the highest activity of 0.47 U/mL U/mL. Based on the 16S rDNA sequence, isolate A8 was identified as Staphylococcus saprophyticus 36QC2CO with a similarity of 98.04%, isolate C7 was identified as S. saprophyticus WWi54 with a similarity of 97.81%, and isolate C8 was identified as Staphylococcus epidermidis 2322 with a similarity of 97.6%.

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