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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Jurnal Biodjati PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat J-Dinamika: Jurnal Pengabdian Kepada Masyarakat ZOOTEC

Tri Ardyati

Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya,

Author-ID : 231510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mareâ€™s Milk Sumbawa Mulyawati, Alifia Issabella; Ardyati, Tri; Jatmiko, Yoga Dwi
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4194

Â Â Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mareâ€™s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations.Â

Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae Wiratno, Ekwan Nofa; Ardyati, Tri; Wardani, Agustin Krisna
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Graduate School, Universitas Brawijaya

Indonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxygen with molasses by Saccharomyces cerevisiae (SAF Instant). Step of this research consist of determination of reducing sugar, ethanol fermentation, total nitrogen determination, ethanol determination and data analysis. Treatment of reducing sugar (GR) and total nitrogen (N) (g.L-1) that are GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 and GR 125 N 10. Fermentation was carried out for 72 hours with three replications. Observation parameters every 24 hours are ethanol and reducing sugar concentration, temperature, acidity and dissolved oxygen. Highest ethanol resulted from GR 125 N 6 (3.68 g.L-1) and GR 100 N 6 (3.53 g.L-1). Low reducing sugar consumption inhibited by by-product of yeast metabolism and molasses chemical compound, lead leaves high sugar concentration (> 80 g.L-1). GR 100 N 6 and GR 125 N 6 have highest fermentation efficiency (69 and 57 %). There was no increase in temperature and decrease in pH significantly (α>0.05). Dissolved oxygen decreased significantly (α>0.05) at the early of fermentation and decrease until the end of fermentation. Total nitrogen 6 g g.L-1 has the highest fermentation efficiency. Keywords: ethanol, molasses, reducing sugar, Saccharomyces cerevisiae, total nitrogen

Antimicrobial and Antioxidant Activity of Endophyte Bacteria Associated with Curcuma longa Rhizome Sulistiyani, Sulistiyani; Ardyati, Tri; Winarsih, Sri
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Most cases of bacterial resistance towards antibiotics, encourage various efforts to gain new sources of antibiotics. Endophyte bacteria is a microorganism has important role as the producer of bioactive compounds. Endophyte bacteria from Curcuma longa with antimicrobial and antioxidant activities have not been studied yet. Curcuma longa has been utilized as the main ingredients of traditional herbal medicines (jamu). The objective of this research was to investigate the antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. Based on morphological characteristics of bacterial colonies, eight endophyte bacteria was isolated from Curcuma longa rhizome. Screening of endophyte isolate has antimicrobial activity was done using agar well diffusion method. The culture supernatant of each endophyte isolate was dropped on agar well against pathogenic bacteria Salmonella enterica ser. Typhi, Staphylococcus aureus and yeast Candida albicans. Three endophyte isolates K3, K2 and M1b showed antimicrobial activity against pathogenic bacteria and yeast. Isolate K3 showed strong antimicrobial activity against C. albicans and S. aureus, however, isolate K2 and isolate M1b showed antimicrobial activity against Salmonella enterica ser. Typhi and S. aureus, respectively. Those endophyte bacteria also had antioxidant activity shown by scavenging ability toward DPPH radical with consecutive percentage of isolate K3 (72.3 %), K2 (51.3 %) and M1b (64.6 %). Isolate K3 showed the highest antimicrobial and antioxidant activity. Based on biochemical characteristics using Microbact 24E kit, isolate K3 was identified as Paenibacilus alvei and isolate K2 as Enterobacter agglomerans. Keywords: antimicrobial, antioxidant, Curcuma longa's rhizome, endophyte bacteria.

Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria Ishak, Ismat; Ardyati, Tri; Aini, Luqman Qurata
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Tidore Island in North Maluku Province is one of the clove (Syzygium aromaticum) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 μgL-1), phosphate solubilization (8.37 μgL-1), and ammonium production (11.71 μgL-1), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia, and Stenotrophomonas. Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.

Evaluation of Proteolytic and Chitinolytic Activities of Indigenous Bacillus Species from Crab Shell Waste Dliyauddin, Moh; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

The increase of crab meat export activities in Indonesia leads to the accumulation of crab shell waste in a massive amount that can naturally degrade and causing bad odor. Naturally, microorganisms will degrade this waste through fermentation and enzymatic reaction, including protease and chitinase due to its high content of chitin and protein. Bacillus is the most potential bacteria to degrade crab shell waste, and indigenous Bacillus from this waste can increase the degradation rates. The aims of this study were to evaluate the proteolytic and chitinolytic activities of indigenous Bacillus species from crab shell waste. Bacillus cereus BP14 and Bacillus licheniformis CK20 as the chitinolytic bacteria, and Bacillus subtilis AP9 and Bacillus licheniformis AP5 as the proteolytic bacteria were isolated from crab shell waste and identified based on the 16S rDNA sequences. The bacterial isolates were grown in skim milk broth for proteolytic characterization and colloidal chitin broth for chitinolytic characterization. The growth rates of each bacteria were determined through the growth curves. The enzymatic activities were determined based on the international standard for protease and chitinase enzyme activities together with growth curve sampling to determine the best incubation time for obtaining the highest enzymatic activities. From the shortest to the longest generation times of the Bacillus species obtained were B. subtilis AP9, B. licheniformis CK20, B. cereus BP14, and B. licheniformis AP5, respectively. The best incubation time for producing the highest enzymatic activity varied among species. However, the end of the logarithmic phase was similar. All of the Bacillus species obtained from this study exhibited chitinolytic and proteolytic activity. Therefore, it can be used as promising candidates of biodegradation agents inenhancing the degradation rates of crab shell wastes.

Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara Daten, Helena; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.04

The research aimed to isolate, characterize, and analyze the ability of lactic acid bacteria (LAB) potential as probiotics to produce hydrolase enzyme. The LAB was isolated using MRS agar by the spread plate method. The LAB characterization includes antimicrobial activity, tolerance to low pH, bile salt, salinity, autoaggregation properties, and ability to produce hydrolytic enzymes. The isolate which has the highest ability to inhibit Aeromonas hydrophila is KBP 3.3, while the isolate which inhibits the highest Streptococcus agalactiae is KBP 1.1.1. The KBP 3.3 and KBP 1.1.1 were able to survive at pH 1 for 24 hours with a survival rate of 93.6% and 98.3%. The KBP 3.3 and KBP 1.1.1 are tolerant to 7.5% bile salt concentrations for 24 hours of 99.46% and 99.11%. The KBP 3.3 is tolerant to 0.5 % salinity for 24 hours with the highest survival rate of 113.38%, while KBP 1.1.1 is 94%. The KBP 3.3 and 1.1.1 have autoaggregation properties of 92.18% and 87.84%. The KBP 3.3 produced the highest lipase enzyme, while KBP 1.1.1 produced the protease enzyme.Keywords: hydrolytic enzyme, lactic acid bacteria, mackerel, probiotic

Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest Pratiwi, Esti Rizkiana; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.07

Plant Growth Promoting (PGP) Endophytic bacteria are used as an alternative biofertilizer to support soil health and plant productivity. This research aimed to isolate, analyze the potential, and identify the endophytic bacteria of Robusta and Arabica coffee plants as biofertilizer agents. Endophytic bacteria were isolated from the roots of coffee plants and tested for their potential to produce IAA, phosphate-solubilizing, and nitrogen fixation. Potential endophytic bacterial isolates were identified based on 16S rDNA sequence similarity. Total isolates from Robusta coffee consisting of ten IAA-producing bacteria, eight phosphate-solubilizing, and seven nitrogen fixation bacteria isolates. Total isolates from Arabica coffee roots were 12 isolates of IAA-producing bacteria, seven isolates of phosphate-solubilizing bacteria, and six isolates of nitrogen fixation bacteria. The highest potential of the isolate from Robusta roots was SS.E2 isolate to produce IAA 110.73 μg.mL-1; SS.P3 isolate to dissolve phosphate 4.42 μg.mL-1, and SS.N2 isolate to produce ammonium 3.15 μg.mL-1. The highest potential of the isolate from Arabica roots was SW.E9 isolate to produce IAA up to 257.16 μg.mL-1; SW.P5 isolate to dissolve phosphate up to 4.55 μg.mL-1; and SW.N6 isolate to produce ammonium up to 1.16 μg.mL-1. Isolates SS.E2, SW.E9, SS.P3, SW.P5, SS.N2, and SW.N6 were respectively identified as Bacillus cereus ATCC 14579, Bacillus cereus ATCC 14579, Rahnella aquatilis B35, Kluyvera intermedia TPY16, Rahnella aquatilis B35, and Pseudomonas tolaasii NCPPB 2192. Potential PGP isolates can be developed as biofertilizer agents for the coffee plant. Keywords: Coffee, Endophytic bacteria, IAA, Nitrogen, Phosphate

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia Faizah, Mamluatul; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.09

Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82 - 1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence. Keywords: Burkholderia cepacia, cellulolytic bacteria, cellulase enzyme, sago waste.

Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Kimbal, Angie Via Resty; Jatmiko, Yoga Dwi; Ardyati, Tri
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.

Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Hasanah, Uswatun; Ardyati, Tri; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.

Co-Authors AGUSTIN KRISNA WARDANI Anggraini, Ayu Arsyi Arie Srihardyastutie Baital Izzatul Badriyah Bambang Poerwadi Chintya Corin Citra Pradani P Dewi Permatasari, Dewi Dian Siswanto Dliyauddin, Moh Dwi Jatmiko, Yoga ekwan nofa wiratno Elok Zubaidah Faizah, Mamluatul Harmoko, Deni Hasria Alang Helena Daten Indah Nur Fitria Irfan Mustafa Ishak, Ismat Jamhari Jamhari Joni Kusnadi Jusup Subagja, Jusup Kimbal, Angie Via Resty LANGKAH SEMBIRING Lisa Lisdiana Luqman Qurata Aini Maulidiyah, Nuris Maulidiyah, Nuris Shobah Mora, Giral Baines Mulyawati, Alifia Issabella Munawaroh Munawaroh Osfar Sjofjan P Purwanto Pratiwi, Esti Rizkiana Purwanto, Agung Putra Ramadhan, Andi Muhammad Faiz Ramadhani, Sulistya Ika Ratna Fadhilah Israwan Restu Nugraha Sri Winarsih Suharjono SULISTIYANI SULISTIYANI Syamsul Arifin Uswatun Hasanah Wahyudi, Aleyda Nur Halizah Wulan Sari Eko Putri YOGA DWI JATMIKO

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