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All Journal Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Health Information : Jurnal Penelitian Journal of Artha Biological Engineering Artha Imperium: Jurnal Pengabdian Kepada Masyarakat
Nurhafid, Mohammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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Journal : Journal of Artha Biological Engineering

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Study of the Characteristics of Bacteria Isolated from the Pelus River, Banyumas Regency Nurhafid, Mohammad; Indyaswari, Marthyana; Husen, Fajar; Riady, Reza Muhammad
Journal Of Artha Biological Engineering Vol. 1 No. 1 (2023)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6wsyrt43

Abstract

Community activities around the Pelus River cause pollution which affects the bacterial community found in the river. The aim of this research was to determine the characteristics of bacteria isolated from the Pelus river environment in Banyumas Regency. The method used in this research is an exploratory method where bacterial sampling is carried out at 3 different stations. The samples taken were then isolated, observed colony morphology, as well as biochemical and physiological characterization of the bacteria. The research results obtained 10 different isolates. A total of 10 isolates were obtained based on morphological differences. The characteristics of most of the Pelus River bacteria show variations ranging from enzymatic to environmental resistance which describes the waters of the Pelus River. Bacteria isolated from the water have characteristics that identify them as bacteria capable of degrading compounds
Rapid Detection of the Aeromonas sp. group using Conventional PCR at the Aquaculture Technology Development Center, Cangkringan, Sleman, Special Region of Yogyakarta Nurhafid, Mohammad; Astuti; Riady, Reza Muhammad; Ufianah; Pardiyanto, Eko; Ruanda, Faldo Ody
Journal Of Artha Biological Engineering Vol. 1 No. 1 (2023)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/c8cxaf15

Abstract

The catfish is a freshwater cultivated fish that can be found all over Indonesia. However, the problems that occur in general are usually attacks by pathogens from the Aeromonas sp group. This research aims to optimize the rapid detection of the Aeromonas sp group using PCR at the Aquaculture Technology Development Center, Cangkringan, Sleman, special region of Yogyakarta. The research was conducted by survey with purposive sampling. Isolation was carried out using two growth media, namely Tryptone Soy Agar (TSA) and Glutamate Starch Phenile (GSP). Colonies that had characteristics of the Aeromonas sp group were subjected to PCR amplification using specific primers for the Aeromonas sp group. The results of the amplification resulted in DNA that matched the target, namely 953bp, which showed that the sample belonged to the Aeromonas sp group. Based on this technique, the level of accuracy in detecting pathogens is higher than conventional methods.
Co-Authors Anandasari, Mira Adyla Anbari, Ilham Astuti Ayal, Esther Lourence Brendha Dini Ryandini Endah Wahyuni Fajar Husen Farhati, Ismi Fitriadi, Ren Handayani, Sekti Hapsari, Dyah Ayu Kunti Hediningsih, Yekti Indyaswari, Marthyana Kasprijo, Kasprijo Muhammad Riady, Reza Palupi, Mustika Pardiyanto, Eko Peni, Wiwik Purnomo Purnomo Riady, Reza Muhammad Riviani Ruanda, Faldo Ody Soedibya, Petrus Hary Tjahja Solly Aryza Sulistiyanto, Hendrik Sutanto Ufianah