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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production
I Arifiantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Improvement of frozen semen quality of Garut Sheep through the addition of α-tocopherol into yolk egg-skim milk diluent ., Herdis; ., Kusuma; Surachman, M; Sutama, I.K; Riza, M; Inounu, I; Purwantara, B; Arifiantini, I
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 1 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.408 KB) | DOI: 10.14334/jitv.v7i1.269

Abstract

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen.   Key words: Antioxidant, sperm, Garut sheep
The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender ., Yudi; Arifiantini, I; Purwantara, B; Yusuf, T.L
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.697 KB) | DOI: 10.14334/jitv.v13i1.593

Abstract

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulos
Comparative Study on the Quality of Friesian Holstein Frozen Semen Treated with Extender from Various Artificial Insemination Centre in Indonesia Arifiantini, I; Yusuf, TL; D, Yanti
ANIMAL PRODUCTION Vol 7, No 3 (2005): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The aim of this research was to compare the quality of Friesian Holstein (FH) frozen semen bull using extender from various artificial center in Indonesia.  Fifteen ejaculates from 3 FH bulls were collected using artificial vagina twice a week.  Collected semen then evaluated macro and microscopically.  Good quality semen diluted using 4 different extenders which was extender from artificial insemination center I (BIB I); II (BIB II); commercial extender Andromedâ (Minitab, Germany) based on Soya lecithin (KK) and tris egg yolk extender (TF), original receipt from Faculty of Veterinary Medicine, IPB.  Extended semen then packed into minitub straws 0.3 ml with the concentration of 25 million sperm each.  The straw then equilibrated at 4 oC for 4 hours, freeze in the liquid nitrogen vapor for 10 minutes and the stored in liquid nitrogen container -196oC.  After 24 hours, semen then thawed at 37oC for 30 second.  Percentage of sperm motility, live and morphology were observed on raw, diluted post equilibrated and post thaw semen respectively.  The data were analyzed using statistical analysis system (SAS) 6.12 version.  Result showed that diluted and after equilibrated semen diluted with BIB II extender was significantly lower (P<0.01) on the percentage of sperm motility than other extender.   The highest percentage was KK extender (57.90%), BIB I (52.09%), TF (46.36%), and BIB II (26.09%).  The influence of different extender on the percentage of live sperm was significantly different (P<0.05) after equilibration.  BIB II showed the lowest percentage compare with other extender.  After semen were thawed, percentage of live sperm shows highly significant different among extender (P<0.01).  The highest percentage of live sperm was KK (72.76%), BIB I (69.77%), TF (64.05%), and BIB II (51.28%) respectively.  The highest percentage of sperm abnormality was on KK extender (6.98%) and the lowest was on TF extender (4.78%).  Based on the material of frozen semen calculation per straw, the most expensive  straw was BIB I (Rp 1158.17); followed by KK (Rp 715.00); TF (Rp 671.35 ) and  BIB II (Rp 653.52).   In conclusion, this result indicate that KK extender has a good post thawed sperm quality with the moderate price. (Animal Production 7(3): 168-176 (2005) Key Words: Artificial Insemination, Thawing, Lecithin, Extender
Karakteristik Semen Segar dan Kualitas Semen Cair Kuda dalam Pengencer Dimitropoulos yang Disuplementasi dengan Fruktosa, Trehalosa dan Rafinosa . Yudi; I Arifiantini; B Purwantara; T L Yusuf
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.553 KB)

Abstract

The objective of the experiment was to study the characteristics of stallion fresh semen and the quality of sperm preserved in Dimitropoulos extender (DV) supplemented with different concentration of fructose, trehalose and raffinose. Semen were collected using artificial vagina from three stallions. Semen characteristics and quality were evaluated macro- and microscopically. Prior to extension, semen were centrifugated at 3000 rpm for 20 minutes. The condensed sperm were re-suspended in DV supplemented with different types of carbohydrate to meet the concentration of 200 million spz/ml. All samples were stored at room and chilled temperature, and were evaluated for motility and viability every 3 h and 12 h. The results of the experiments indicated that fresh semen characteristics were fair good; the volume, consistency, motility, live-dead ratio, concentration (106/ml), total spermatozoa (109/ejaculate) and abnormality were 29.25±9.33 ml, watery, 7.00±0.12, 67.08±9.08%, 77.89±6.46%, 211.88±21.15, 6.28±2.45 and 27.26±4.64%, respectively. The supplementation of different type and concentration of carbohydrates did not significantly affect the motility and viability. However, the supplementation of 50 mM fructose significantly increased the motility and viability of the sperm compared to the control. In conclusion, carbohydrate supplementation in DV may not maintain the sperm quality, particularly in the medium with the osmolarity higher than 400 mOsm/kg. Key words: stallion, semen, Dimitropoulos, carbohydrate
Kualitas Semen Beku Kuda dalam Pengencer Susu Skim dan Dimitropoulos dengan Dimetilformamida Sebagai Krioprotektan I Arifiantini; I Supriatna; . Aminah
Media Peternakan Vol. 30 No. 2 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Equine semen are far less tolerant in the freezing and thawing process than bull semen. The stallion spermatozoa are known susceptible to cold-shock relating with the content of their fatty acid on the plasma membrane. The extender is one of determining factors in the success of stallion semen cryopreservation, as an energy source and protector the cell from harmfull effect of cold shock. The common cryoprotective agent (CPA) for mammalian spermatozoa was glycerol, but for stallion semen cryopreservation, dimethyl formamide (DMF) was more suitable. This research was conducted to compare the success of the stallion semen cryopreservation in skim milk and dimitropoulos (DV) extender with DMF as cryoprotectant. Semen from three sexualy mature stallions was collected twice a week using an artificial vagina. Semen was evaluated macro- and microscopically and then divided into two tubes, diluted each of them with skim milk dan DV extender (1:1), centrifuged at 3 000 rpm for 15 minutes. The supernatant was removed and the pellet (spermatozoa) was re-diluted in skim DMF (SDMF) and DVDMF extender with the concentration of spermatozoa was 200x106 ml-1. The semen then packed in 0.3ml minitub straw equilibrated for two hours at 4-50C and frezee in liquid N2 vapor for 10 minutes. The assessment of sperm quality was conducted based on the percentage of sperm motility and viability. In this research, post-thawed semen in DVDMF showed the percentages of the motility (36.2%) and the viability (59.3%) higher (P < 0.05) than SDMF (28.5 and 48.0 %). In conclusion, the DVDMF extender provided better post-thawed semen quality than SDMF. Key words: cryopreservation, stallion semen, skim, dimitropoulos, dimethyl formamide
The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender Yudi .; I Arifiantini; B Purwantara; T.L Yusuf
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.697 KB) | DOI: 10.14334/jitv.v13i1.593

Abstract

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulos
Improvement of frozen semen quality of Garut Sheep through the addition of α-tocopherol into yolk egg-skim milk diluent Herdis Herdis; Kusuma Kusuma; M Surachman; M Riza; I K Sutama; I Inounu; B Purwantara; I Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.408 KB) | DOI: 10.14334/jitv.v7i1.269

Abstract

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen. Key words: Antioxidant, sperm, Garut sheep
Co-Authors . Yudi Aminah Aminah B Purwantara B Purwantara Herdis . herdis herdis I Inounu I K Sutama I Supriatna I.K Sutama Kusuma . Kusuma Kusuma M Riza M Surachman T L Yusuf T.L Yusuf TL Yusuf, TL Yanti D, Yanti Yudi .