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Wasissa, Madarina
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Biochemistry, Genetics & Molecular Biology Veterinary

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Variasi Karakter Resistensi Antibiotik Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Ettawa di Yogyakarta Lestari, Fajar Budi; Artdita, Clara Ajeng; Wasissa, Madarina; Hidayah, Nurulia
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.52529

Abstract

Salah satu penyakit yang sering dijumpai dalam budidaya kambing Peranakan Etawa (PE) adalah mastitis. Beberapa bakteri patogen dapat menyebabkan mastitis diantaranya adalah Staphylococcus sp., E. coli, Klebsiella spp., dan Streptococcus spp. Meningkatnya kejadian penyakit menyebabkan meningkatnya penggunaan antibiotik yang berakibat pada peningkatan potensi resistensi dan residu antibiotik. Penelitian ini bertujuan untuk mengetahui variasi karakter fenotipik resistensi bakteri penyebab mastitis yang diisolasi dari susu kambing PE yang mengalami mastitis subklinis terhadap berbagai golongan antibiotik yang umum dipakai petugas medis veteriner di wilayah Yogyakarta. Sebanyak 8 isolat diperoleh dari peternakan rakyat di wilayah kecamatan Kokap, Kulon Progo,, sedangkan 4 isolat diperoleh dari wilayah Pakem, Sleman, Yogyakarta. Dua belas isolat tersebut terdiri dari 3 jenis bakteri yang berbeda yaitu Staphylococcus sp., Escherichia coli, dan Klebsiella sp. Berdasarkan hasil uji antibiogram diketahui bahwa terdapat perbedaan pola resistensi antibiotik antara isolat bakteri penyebab mastitis dari Kulon Progo dan Sleman. Ampicillin, penicillin G, tetracyclin, dan cefoxitin efektif digunakan untuk pengobatan mastitis di daerah Sleman, sedangkan di Kulon Progo gentamicyn, tetracyclin dan erythromycin adalah pilihan antibiotik yang efektif digunakan
Antibiotic Resistance Detection of Staphylococcus aureus Isolated from Dairy Cattle and Pet Animals Yosyana, Alyaa Rifqoh Putri; Salasia, Siti Isrina Oktavia; Wasissa, Madarina; Alhadz, Ghias Ghifari; Aziz, Fatkhanuddin
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100211

Abstract

Antibiotics play a significant role in controlling bacterial infection, however, will no longer be effective because of antimicrobial resistance (AMR). Staphylococcus aureus has become resistant to various antibiotics, so detecting and analyzing genes encoding antibiotic resistance traits is important. This research aims to identify phenotypically, antibiotic sensitivity, and detect antibiotic-resistant genes in S. aureus isolated from dairy cattle and pet animals. Samples from dairy farms in Boyolali total of 30 samples and 62 samples of pet animals in Yogyakarta and Semarang were used. Phenotypic and genotypic identification results based on 23S rRNA and nuc genes showed 80% (24/30) dairy milk samples and 19,35% (12/62) pet animal samples were S. aureus positive. Based on antibiotic susceptibility test, dairy milk S. aureus isolates are resistant to penicillin G (50%), oxacillin (25%), tetracycline (21%), ampicillin (17%), gentamicin, cefoxitin, and amoxicillin (13%), clindamycin (4%), and still sensitive to erythromycin (100%). Pet animal S. aureus isolates showed resistance to oxacillin and erythromycin (13,3%), tetracycline, penicillin G, and clindamycin (6,67%), but still sensitive to gentamicin, ampicillin, cefoxitin, and ciprofloxacin and amoxicillin (100%). These results showed S. aureus dairy milk and pet animal isolates phenotypically have resistance almost 50% to various antibiotics but are still sensitive to erythromycin. The result of this research indicated there are majority of multidrug-resistant S. aureus strains in dairy milk and pet animals threaten public health. These results can be used as a basic strategy for controlling and preventing multidrug resistance in S. aureus.
Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta Alhadz, Ghias Ghifari; Salasia, Siti Isrina Oktavia; Yosyana, Alyaa Rifqoh Putri; Wasissa, Madarina; Lestari, Fajar Budi; Widayanti, Rini
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100240

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP),  are known as bacterial  resistant to various antibiotics. MRSA transmission occurs between humans and direct human contact with dogs and cats. This study aimed to isolate, characterize, and analyze bacterial resistance to antibiotics phenotypically and genotypically from clinical case samples of pet animals in Yogyakarta. Bacterial isolates were identified through biochemical tests and molecular identification by species-specific 23S rRNA and nuc genes with polymerase chain reaction (PCR) and pta genes with PCR-restriction fragment length polymorphism (RFLP).  Bacterial resistance to several antibiotics was analyzed using disk diffusion Kierby-Bauer method. The occurrence of methicillin resistance were screened by oxacillin resistance screening agar base (ORSAB) and confirmed by detection of the mecA gene with PCR. Based on the phenotypic and genotypic identification showed that 11 samples were identified as S. aureus (6 isolates) and S. pseudintermedius (5 isolates). The resistance tests revealed that 36% were resistant to tetracycline, cefoxitin (27%), oxacillin and erythromycin (9%).  Screening of methicillin resistance with ORSAB indicated that all isolates were methicillin resistances (100%). Detecting the mecA gene encoding methicillin resistance showed that 81.81% of isolates were resistant to methicillin, with detail S. aureus (all 6 isolates) and S. pseudintermedius (4 isolates). Based on the antibiogram, ORSAB, and mecA gene detection, it could be confirmed that three isolates were MRSP, and six isolates were MRSA. The results of this study indicate the high occurrence of methicillin-resistant strains in pets that have the potential zoonotic spread to other animals and humans.
Combined molecular and clinicopathologic characterization of feline coronavirus infection in cats from a multicat shelter Amalia, Fia; Wasissa, Madarina; Salasia, Siti Isrina Oktavia; Lestari, Fajar Budi
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.259-271

Abstract

Molecular detection of feline coronavirus (FCoV) is essential for diagnosing feline infectious peritonitis (FIP). This study aimed to confirm FCoV infection in clinically suspected cats and to describe the associated hematological and cytological changes. Reverse transcriptase polymerase chain reaction (RT-PCR), targeting the N gene, was performed on 20 cats exhibiting clinical signs consistent with FCoV infection, including vomiting, diarrhea, abdominal effusion, rhinitis, uveitis, and ataxia, yielding the expected 386 bp amplicon in all cases. Ascitic fluid was preferred for RT-PCR when available, whereas blood samples were analyzed for hematology. Hematologic profiles revealed consistent inflammatory changes. The mean total leukocyte count was 22.8 ± 12.1 × 10³/µL, and the mean platelet count was 769 ± 505 × 10³/µL. Neutrophilia and leukocytosis were the most common abnormalities, observed in 55% (11/20) of cats, followed by monocytosis in 15% (3/20), lymphopenia in 5% (1/20), and lymphocytosis in 30% (6/20). Platelet counts showed marked variation, ranging from severe thrombocytopenia in 40% (8/20) to extreme thrombocytosis in 35% (7/20). Cytologic examination of ascitic fluid from five cats revealed macrophage-predominant exudates with moderate numbers of neutrophils and few lymphocytes. Macrophages were often vacuolated and contained phagocytosed debris, whereas some neutrophils appeared degenerated. The background consisted of proteinaceous material consistent with a high-protein effusion. The integration of molecular, hematologic, and cytologic findings provides a multimodal diagnostic approach that improves diagnostic reliability. These findings emphasize the importance of combining PCR with clinicopathological data to enhance the early and accurate detection of FIP in multicat environments.
Co-Authors Alhadz, Ghias Ghifari Amalia, Fia Clara Ajeng Artdita, Clara Ajeng Fajar Budi Lestari Fatkhanuddin Aziz, Fatkhanuddin Hidayah, Nurulia Rini Widayanti Siti Isrina Oktavia Salasia Yosyana, Alyaa Rifqoh Putri