Article Per Year (5 Year)
p-Index From 2020 - 2025
0.947
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Indonesian Journal of Biotechnology The Indonesian Biomedical Journal Jurnal Kedokteran Hewan Organisms: Journal of Biosciences
Siti Isrina Oktavia Salasia
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
Determination of Haemaglutinin and Gene Encoding Fibronectin Binding Proteins Staphylococcus aureus Isolated from Dairy Milk Cows Pratomo, Feny Prabawati; Salasia, Siti Isrina Oktavia; Tato, Syarifudin
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbstractStaphylococcus aureus is a major pathogen causing clinical and subclinical mastitis in dairy milk cows. The mastitis has immense economical impacts, where it reduces of the quantity and quality of milk production. The aims of the research were to analyse haemaglutinin and gene encoding fibronectin binding proteins. Nineteen Staphylococcus aureus isolates used in the present study were isolated from dairy milk cows from Yogyakarta, Solo, Boyolali and Sumedang. The haemagluitinin of S. aureus were determined based on haemaglutination reaction to erythrocytes of rabbit. Detection of gene encoding fibronectin binding proteins could be performed with specific primers using polymerase chain reaction (PCR). The results of studies showed that most of S. aureus (78,95%) expressed haemaglutinin based on their ability to aglutinate rabbit erythrocytes. Analysis of gene encoding fibronectin binding proteins of S. aureus revealed gene fnbA with size of approximately 1300 bp for 57,89% isolates, gene fnbB with size of approximately 900 bp for 31,58% isolates and both of gene fnbA and fnbB could be detected for 31,58% isolates. The characters of S. aureus based on haemaglutinin, gene fnbA and fnbB of the present study could be used as an information to control of S. aureus infection in dairy herds.Keywords : Staphylococcus aureus, haemaglutinin, gene encoding fibronectin binding protein, milk cow
Characterization of Haemolysin of Staphylococcus aureus Isolated from Food of Animal Origin Ariyanti, Dwi; Salasia, Siti Isrina Oktavia; Tato, Syarifudin
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.885 KB)

Abstract

Staphylococcus aureus is an important pathogen bacteria causing food poisoning and various infection in animals and humans. Haemolysin is one of the virulence factors of Staphylococcus aureus. The aims of the research were to characterize haemolysins of Staphylococcus aureus isolated from various food of animal origin, phenotypic- and genotypically. In the present study, eleven Staphylococcus aureus isolated from various food of animal origins from traditional markets and supermarkets in Yogyakarta, Sidoarjo, Jakarta, and Bandung were characterized for haemolysin, pheno- and genotypically. Characterization of haemolysin phenotypically based on haemolysis pattern of Staphylococcus aureus on sheep blood agar plate. Genes encoding hemolysin were amplified with specific primers by using polymerase chain reaction (PCR) technique. The results of the studies showed that Staphylococcus aureus on sheep blood agar plates revealed an alpha haemolysis pattern (18,18%), beta haemolysis (27,27%) and gamma haemolysis (54,55%). Based on amplification of the gene encoding haemolysin of Staphylococcus aureus with specific primers showed hla genes (81,81%), and hla combined with hlb genes (18,18%). The amplification of gene hla and hlb had a single amplicon with a size of approximately 534 bp and 833 bp, respectively. The haemolysin characteristics of Staphylococcus aureus from various food of animal origin could be used as important information to control staphylococcal food poisoning.Keywords : Staphylococcus aureus, haemolysin, PCR, food of animal origins
Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Abstract

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.  
Antibiotic Resistance Detection of Staphylococcus aureus Isolated from Dairy Cattle and Pet Animals Yosyana, Alyaa Rifqoh Putri; Salasia, Siti Isrina Oktavia; Wasissa, Madarina; Alhadz, Ghias Ghifari; Aziz, Fatkhanuddin
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100211

Abstract

Antibiotics play a significant role in controlling bacterial infection, however, will no longer be effective because of antimicrobial resistance (AMR). Staphylococcus aureus has become resistant to various antibiotics, so detecting and analyzing genes encoding antibiotic resistance traits is important. This research aims to identify phenotypically, antibiotic sensitivity, and detect antibiotic-resistant genes in S. aureus isolated from dairy cattle and pet animals. Samples from dairy farms in Boyolali total of 30 samples and 62 samples of pet animals in Yogyakarta and Semarang were used. Phenotypic and genotypic identification results based on 23S rRNA and nuc genes showed 80% (24/30) dairy milk samples and 19,35% (12/62) pet animal samples were S. aureus positive. Based on antibiotic susceptibility test, dairy milk S. aureus isolates are resistant to penicillin G (50%), oxacillin (25%), tetracycline (21%), ampicillin (17%), gentamicin, cefoxitin, and amoxicillin (13%), clindamycin (4%), and still sensitive to erythromycin (100%). Pet animal S. aureus isolates showed resistance to oxacillin and erythromycin (13,3%), tetracycline, penicillin G, and clindamycin (6,67%), but still sensitive to gentamicin, ampicillin, cefoxitin, and ciprofloxacin and amoxicillin (100%). These results showed S. aureus dairy milk and pet animal isolates phenotypically have resistance almost 50% to various antibiotics but are still sensitive to erythromycin. The result of this research indicated there are majority of multidrug-resistant S. aureus strains in dairy milk and pet animals threaten public health. These results can be used as a basic strategy for controlling and preventing multidrug resistance in S. aureus.
Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta Alhadz, Ghias Ghifari; Salasia, Siti Isrina Oktavia; Yosyana, Alyaa Rifqoh Putri; Wasissa, Madarina; Lestari, Fajar Budi; Widayanti, Rini
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100240

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP),  are known as bacterial  resistant to various antibiotics. MRSA transmission occurs between humans and direct human contact with dogs and cats. This study aimed to isolate, characterize, and analyze bacterial resistance to antibiotics phenotypically and genotypically from clinical case samples of pet animals in Yogyakarta. Bacterial isolates were identified through biochemical tests and molecular identification by species-specific 23S rRNA and nuc genes with polymerase chain reaction (PCR) and pta genes with PCR-restriction fragment length polymorphism (RFLP).  Bacterial resistance to several antibiotics was analyzed using disk diffusion Kierby-Bauer method. The occurrence of methicillin resistance were screened by oxacillin resistance screening agar base (ORSAB) and confirmed by detection of the mecA gene with PCR. Based on the phenotypic and genotypic identification showed that 11 samples were identified as S. aureus (6 isolates) and S. pseudintermedius (5 isolates). The resistance tests revealed that 36% were resistant to tetracycline, cefoxitin (27%), oxacillin and erythromycin (9%).  Screening of methicillin resistance with ORSAB indicated that all isolates were methicillin resistances (100%). Detecting the mecA gene encoding methicillin resistance showed that 81.81% of isolates were resistant to methicillin, with detail S. aureus (all 6 isolates) and S. pseudintermedius (4 isolates). Based on the antibiogram, ORSAB, and mecA gene detection, it could be confirmed that three isolates were MRSP, and six isolates were MRSA. The results of this study indicate the high occurrence of methicillin-resistant strains in pets that have the potential zoonotic spread to other animals and humans.
Ultraviolet B (UVB) Radiation Induces Skin Alterations, Emperipolesis and Decreases the Erythroid-to-myeloid Ratio in Rats Farida, Verda; Dewananda, Dion Adiriesta; Sahid, Muhammad Novrizal Abdi; Salasia, Siti Isrina Oktavia
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3227

Abstract

BACKGROUND: Ultraviolet B (UVB) radiation is commonly known to be related to skin inflammation. The inflammation process is orchestrated by many cell types, including immune cells. Changes in bone marrow cellularity can also be an indicator of inflammation. Megakaryocytes, myeloid immune cells progenitor and erythroid progenitor cells, are at high risk of changes upon UVB irradiation. However, there are still limited study observing the change of bone marrow cell population after UVB irradiation. Therefore, this study was conducted to investigate the alteration of the skins, emperipolesis, and the change in erythroid and myeloid cell population in bone marrow after UVB irradiation.METHODS: Ten Wistar rats were divided equally into control and UVB-irradiated group. The skin superficial condition before and after UVB irradiation was observed with a skin analyzer camera. On the 9th day, skin tissues were processed for the observation of general skin structure with hematoxylin-eosin (HE) staining, mast cells infiltration with toluidine blue staining, and collagen fibers with Mallory staining. Bone marrow and peripheral blood samples were collected and proceeded for Giemsa-staining to observe the cell population.RESULTS: Erythema appeared on the skin as marked by orange-red spots. There were hyperkeratosis and pigment accumulations in the skin of UVB-irradiated group. The depletion of collagen-density and hemorrhage were clearly observed in the skin of UVB-irradiated group. There were higher mast cell numbers in the UVB-irradiated skin compared to non-treated skin. The erythroid-to-myeloid ratio in the bone marrow was decreased to around 1.6:11.2 from the normal ratio of 1:4. In addition, emperipolesis was observed in the bone marrow induces by UVB-irradiation.CONCLUSION: These results indicate that UVB-irradiation alters the skin structure, erythroid-to-myeloid ratio, and induced emperipolesis.KEYWORDS: emperipolesis, erythroid, myeloid, skin, UVB radiation
HUBUNGAN CLONAL METHICILLIN RESISTANT Staphylococcus aureus (MRSA) PADA SAPI DAN MANUSIA Salasia, Siti Isrina Oktavia; Tato, Syarifuddin; Prabawati, Feny; Ariyanti, Dwi
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.924

Abstract

Penelitian ini bertujuan menentukan hubungan clonal methicillin resistance Staphylococcus aureus (MRSA) antara isolat sapi dan manusia.Staphylococcus aureus yang digunakan dalam penelitian ini berasal dari infeksi kulit manusia di Yogyakarta (10 isolat) dan dari susu sapi perah (11 isolat) yang berasal dari Yogyakarta, Solo, dan Boyolali. Identifikasi bakteri dan uji resistensi S. aureus terhadap methicillin telah dilakukan pada penelitian sebelumnya. Hubungan genetika S. aureus antar isolat sapi dan manusia ditentukan menggunakan teknik single enzyme amplified fragment length polymorphism (AFLP). Hasil penelitian diketahui bahwa berdasar analisis AFLP S. aureus memperlihatkan 15 pola genetika (A sampai O) dan dapat dikelompokkan ke dalam 7 klas (I sampai VII). Staphylococcus aureus isolat asal sapi dari daerah yang berdekatan (Boyolali dan Solo), dapat dikelompokkan dalam 1 klas (kecuali 1 isolat sapi dari Yogyakarta), isolat sapi dan manusia dari Yogyakarta dapat dikelompokkan dalam beberapa klas. Masing-masing klas terdapat isolat S. aureus yang telah resisten terhadap MRSA. Hubungan genotipe S. aureus dalam penelitian ini dapat digunakan untuk mengetahui distribusi clonal antara isolat sapi dan manusia dan dapat digunakan sebagai kontrol adanya infeksi MRSA di Indonesia.
Case Report: Clinical Diagnostic Challenges of Hypertrophic Cardiomyopathy (HCM) in a Persian Cat Fitranda, Mulya; Ryadi, Agus Shafiq; Amalia, Fia; Dion Andiriesta Dewananda; Nururrozi, Alfarisa; Salasia, Siti Isrina Oktavia
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 5 No. 2 (2025): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/47y0dt96

Abstract

Hypertrophic Cardiomyopathy (HCM) is a common and clinically significant cardiomyopathies affecting felines characterized by excessive thickening of the left ventricular myocardium and decreased systolic pressure. This report describes the case of a 9-year-old, 4 kg, female Persian cat presented with dyspnea, anorexia, and lethargy. On physical examination, the normal rectal temperature, slight pallor mucous membranes, increases capillary refill time with respiratory distress were observed. Auscultation of the thorax revealed arrhythmia. Blood pressure and mean arterial pressure (MAP) indicated hypertension. Serial blood counts were consistent with anemia, eosinopenia and monocytosis suggesting chronic stress. Thorax radiograph revealed the heart enlargement through vertebral heart scale (VHS) and cardiothoracic ratio (CTR) score measurement. Complementary diagnostic test with biomarker pro-Brain Natriuretic Peptide (proBNP) confirmed the diagnosis of HCM. This case report describes the clinical findings, cardiac evaluation, and HCM diagnosis in a geriatric cat.
Co-Authors Alfarisa Nururrozi, Alfarisa Alhadz, Ghias Ghifari Amalia, Fia Christin Marganingsih Santosa Dewananda, Dion Adiriesta Dion Andiriesta Dewananda Dwi Ariyanti Fajar Budi Lestari Fatkhanuddin Aziz, Fatkhanuddin Feny Prabawati Feny Prabawati Pratomo, Feny Prabawati Mulya Fitranda Rini Widayanti Ryadi, Agus Shafiq Sahid, Muhammad Novrizal Abdi Syarifuddin Tato Syarifudin Tato, Syarifudin Verda Farida Wasissa, Madarina Yosyana, Alyaa Rifqoh Putri