Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

0.23

P-Index

This Author published in this journals

All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu Ternak Veteriner BERITA BIOLOGI

B Purwantara

Unknown Affiliation

Author-ID : 2422054

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Industrial & Manufacturing Engineering Veterinary

Published : 24 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

Improvement of frozen semen quality of Garut Sheep through the addition of α-tocopherol into yolk egg-skim milk diluent Herdis Herdis; Kusuma Kusuma; M Surachman; M Riza; I K Sutama; I Inounu; B Purwantara; I Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen. Key words: Antioxidant, sperm, Garut sheep

Integrity of swamp buffalo sperm on a variety of semen freezing process Herdis .; B Purwantara; I Supriatna; I.G Putu
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 ± 3.70% and 54.34 ± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 ± 3.32% and 43.44 ± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 ± 4.51% and 51.58 ± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization. Key words : Swamp buffalo bulls, sperm integrity, freezing process

SELEKSI DAN KAPASITASI SPERMATOZOA DENGAN METODE PERCOLL GRADIENT VKYUK FERTILISASI OOSIT DAN PRODUKSIEMBRIO IN VITRO PADA SAPI Endang Triwulanningsih; MR Toelihere; TL Yusuf; B Purwantara; K Diwyanto; JJ Rutledge
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1214

This research has been conducted at the laboratory of in vitro fertilization of the University ofWisconsin, USA. These embryos may be used for improving genetic value of Indonesian cattle. Ovaries were collected from slaughterhouse in Wisconsin. Oocytes were matured in TCM-199 medium enriched with FSH 10 \i\lm\, estradiol 17 P lul/ml and 10 % FCS for 20 hours. The oocytes were fertilized in vitro with motile sperm selected and capacitated by using the percoll gradient with 2 ml vs 0.5 ml per layer as treatment A and B respectively. Sperm and oocytes were incubated in fertilization medium (mTALP) for 20 hours. All zygotes were cultured in CRlaa medium up to btastocyst stage and were fed with serum 5 \iV 50 )j.l in culture medium on day 6. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 86.3 vs 91.6 %, 53.3 % vs 75.9 %; 32.6 % vs 63.4 %; 21.1 % vs 33.0 %; 13.7 % vs 8.4 %, 32.9% vs 15.6 % for treatment A (n=1007) vs B (n=1055), respectively. Based on result of this study, it is concluded that the best method for IVP (in vitro production) of cattle embryos is using percoll gradient with 500 ul per layer.

Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing Abdul Samad; D D Solihin; Cece Sumantri; B Purwantara
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3107

The Maleo Senkawor bird (Macrocephalon maleo) is endemic and widely distributed in Sulawesi. Maleo Senkawor bird is monomorphic so it is difficult to distinguish between male and female individuals. This study aimed to determine the kinship relationship among M. maleo in various geographic areas in Sulawesi through the use of the gene marker Cytochrome-b (Cyt-b), determine the sex ratio, and examine the reliability of the 2550F/2718R primer. Fifteen (feather and eggshell) samples from North Sulawesi, Southeast Sulawesi, and Central Sulawesi were collected. Total DNA isolation was performed using the Dneasy® Blood and Tissue kit (Cat. No. 69504) following a Qiagen protocol with modification. PCR amplification (35 cycles) used a forward primer MMCytb_F (5'- GAAAATCCCACCCCCTACTA-3'), a reverse primer MMCytb_R (5'- GTTGGCTACGAGGAGTCAGA-3') and a primer for sexing used 2550F/2718R. Analysis was performed on the length of 903 bp Cyt-b mtDNA gene sequences of M. maleo along with their amino acid sequences (301 AA) and Chromo Helicase DNA-Binding (CHD) gene sequences. Based on the genetic distance of the Kimura 2-Parameter model and p-distance, the interpopulation of M. maleo in Sulawesi (the population of Southeast Sulawesi was separated from the population of Central Sulawesi and North Sulawesi) was 0.002 (0.2%) – 0.003 (0.3%). Each population has formed a different haplotype. Primer 2550F/2718R was able to amplify the CHD gene and could distinguish the sex identification. Male individuals are more dominant than female individuals with a sex ratio of 6,5:1

Co-Authors . Judi . Yudi A Widianti Abdul Samad Aditya Aditya Amrozi Anak Agung Gede Sugianthara Astuti . B Huoso Cece Sumantri D D Solihin D Muchtadi D Sajuthi D Sayuthi D. Sajuthi Deddy Muchtadi Endang Triwulaninngsih Endang Triwulanningsih Fransiska R Zakaria G Semiadi Herdis . herdis herdis Heri Winarsi I Arifiantini I Inounu I K Sutama I Supriatna I-K Sutama I.G Putu J Manansang J.J Rutledge JJ Rutledge K Diwyanto Kusuma Kusuma M Agil M Astawan M Riza M Surachman M.R Toelihere MR Toelihere N.M Artiningsihi P Situmorang R Handarini R I Afiriantini R I Arifiantini R Sudarwati R.K Achjadi Riasari Gail Sianturi S Astuti S Mulyono S Prastiti T L Yusuf T Wresdiyati T.L Yusuf T.L. Yusuf TL Yusuf Wilmientje Marlene Mesang Nalley Y T Hastuti Yudi . Yudi Yudi

Title

Found 24 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 24 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 24 Documents
Search