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Preservation of Bacillus pumilus PU4-2 xylanases by immobilization technique into pollard and cation addition T Haryati; P.A Marbun; T Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Utilization of by-product from agriculture as alternative source of feedstuff has been widely practiced. However their usage is limited due to high fiber content and low nutrient digestibility. The use of specific hydrolizing enzymes, xylanases are gaining importance because of their wide application in various industrial sectors especially in bioconversion of hemicellulosic material. This experiment was done to evaluate the effect of cation addition and immobilization of enzyme into pollard on stability of B. pumilus xylanase. The enzyme extract was purified by precipitation with 75% ammonium sulphate. Four kinds of cation (Ca2+, Fe3+, Mg2+, Zn2+) were added to the purified enzyme, at concentration of 1m M and stored at 4 and 27˚C. For immobilization process, the optimum enzyme concentration that will be added to pollard has been evaluated by analysis of xylanase activity and their recovery. The specific activity of enzyme after precipitation increased 1.8 times, from 420.3 to 765.2 U/mg protein. All cations act as activator which relative activity become 130.6; 139.0; 103.8 and 163.5% respectively. Concentration of 0.5mM Ca2+ and Fe3+ were most able to keep xylanases activity stable at 4˚C. The optimum composition of enzymes and pollard was 1.5 ml for 5 gram of pollard with recovery of xylanases activity of 82.2%. In immobilized enzyme, the activity of enzyme without cation addition is higher than that with addition of Ca2+ and Fe3+. Activity of enzyme stored at 4˚C is more stable than that at 27˚C. Immobilized enzyme is more stable for storage, which lasted for 7 weeks at 27˚C and 12 weeks at 4˚C compared to liquid enzyme which lasted for only 7 days at 27˚C and 13 days at 4˚C. Key words: Xylanase, Bacillus pumilus PU4-2, Preservation, Imobilization, Cation

Utilization off fermented copra meal with A. niger in broiler diet T Haryati; M.H Togatorop; A.P Sinurat; T Purwadaria; Murtiyeni .
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Research of nutritional evaluation of fermented copra meal and its application in broiler has been conducted. In the first experiment the crude protein and fiber contents were determined in the fermented copra meal using A. niger BPT, NRRL 337 and ES1. Further, 384 of Indian River DOC were used for in vivo experiment. Completed Randomized Design was used with 8 treatments: Control without and with 10% addition of copra meal, 3 levels of fermented copra meal addition with Aspergillus niger BPT or A. niger NRRL 337 (10, 15, 20%). Every treatment was repeated 8 time with 6 birds each. Variables measured were body weight, consumption, feed conversion, mortalities and carcass analyses. Diets were formulated in isocalori and isoprotein. The research was conducted in 6 weeks. The results showed that fermentation process increased the copra meal protein content from 22.3 to 36.1; 35.6 and 38.6% respectively for fermentation with A. niger BPT, NRRL 337 and ES1, decreased the crude fiber from 19.5 to 13.0; 18.9 and 8.28%. Results during 3 weeks trial show that the treatment significantly affected on broiler performance (P<0.05). Fermented copra meal was better than non fermented copra meal. There was no significantly different (P>0.05) in broiler performance at 6 weeks trial evaluation. The best conversion was in diet with 10% fermented copra meal with A. niger BPT and NRRL 337. The feed conversion of diets with 10% of each fermented products were better than control or diet without copra meal. The feed conversion of 15% fermented copra meal addition was not significantly different to control. Utilization of fermented copra meal with A. niger BPT or NRRL 337 should not more than 15%. Key Words: Copra Meal, Bioprocess, Broiler

Supplementation of Morinda citrifolia waste as bioactive compound on the performances of broiler I.A.K Bintang; A.P Sinurat; T Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the use of dried Morinda citrifolia waste as feed additive in broiler ration was conducted. One hundred day old chicks (doc) were allocated into 4 levels (control; 0.0; 1.2; 2.4 and 4.8 g/kg feed) of M. citrifolia waste with 5 replication. Each replication had 5 birds. The treatments were allocated in a completely randomized design. Variables measured were: feed intake, live weight, feed conversion ratio (FCR), percentages of carcass and internal organs ( liver. gizard, abdominal fat, and thickness of intestine). The results showed that M citrifolia waste supply did not significantly (P>0.05) affect all variables measured, but feed intake of those fed with high levels (4,8 g/kg) of M. citrifolia waste was significantly (P<0.05) lower than those fed with 1.2 g/kg. FCR was significantly (P<0.05 ) lower as compared to the control; 1.2 and 2.4 g/kg. It is concluded that the best treatment was the supplementation of M. citrifolia waste of 4.8 g/kg, this treatment improved feed efficiency by 5% as compared to the control. Key Words: M. citrifolia Waste, Performances, Broiler

Optimation of β-mannanase production on submerged culture of Eupenicillium javanicum as well as pH and temperature enzyme characterizations T Purwadaria; T Haryati; E Frederick; B Tangendjaja
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Two successive experiments were conducted to determine the optimal substrate concentration of coconut meal (CM) and incubation time for production of β-mannanase from Eupenicillium javanicum. Both experiments were designed based on factorial. In the first experiment, the main factor was substrate concentration of 1, 2, and 3%, while the sub-factor was incubation time of 1, 2, 3, 4, 5, 6, and 7 days. The two factors were interacted highly significantly (P<0.01). Since the highest β- mannanase activity, protein concentration and saccharification activity towards coconut meal were obtained from 3% CM after five day incubation time (P<0.05), the second experiment was designed for higher substrate concentration. The main factor was also substrate concentration of 3, 4 and 5%, while the sub factor was incubation time of 5 and 6 days. The two factors were also interacted highly significantly (P<0.01) for mannanase activity and protein concentration, while specific activity was not significantly different (P>0.05). The best activity was obtained at 4% of coconut meal for five day incubation time, which was not significantly different with that of 3% at the same incubation time. Therefore, it was concluded from both experiments that the best enzyme production was obtained from 3% of coconut meal at incubation time of 5 days. Then, further experiments show that the enzyme had optimum pH at the range of 5.4-5.8, the same pH range in duodenum, while at pH 4.5 the activity was relatively low. Although, at pH 4.5 the enzyme activity was reduced, the enzyme was still active for four hours. At pH 5.8 and 6.5 the enzyme was quite stable. The optimum temperature of the enzyme was at 500C, higher than the body temperature of most poultry (400C). The reduction of enzyme activity at 400C could be overcome by increasing the enzyme concentration. The enzyme was stable after 4 hour incubation at 28 (room temperature) and 400C, however, the enzyme activity was considerably reduced at temperature of 900C after 60 second incubation. In the poultry digestion system the activity is not affected by temperature, but in the pelleting process where the steam temperature approximately 900C has to be limited for not more than 30 seconds. Key words: Coconut meal, β-mannanase, Eupenicillium javanicum, pH and temperature characterization

The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers Tiurma Pasaribu; A.P Sinurat; T Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG) as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD), egg weight, feed consumption, feed conversion ratio (FCR), egg quality comprising yolk colour, albumin and yolk weights, egg shell eight and thikness, and mortality were observed for 24 weeks. The results showed that feed consumption, % HD, egg weight, FCR, yolk colour, albumin weight, yolk weight, egg shell weight, and egg shell thickness were not significantly different (P>0.05) between the control and DG treatment, except for the Haugh unit (HU). Thus, it can be concluded that Aloe vera bioactives has the same effectiveness as antibiotic as a feed additive at the level of commercial farms. Key Words: Laying Hens, Egg Production, Egg Quality, Aloe vera barbadensis Bioactives, Antibiotic

Nutritive value of fermented palm oil sludge added with different sources of phosphorus Tiurma Pasaribu; N Arini; T Purwadaria; A.P Sinurat
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 3 (2003): SEPTEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The experiment has been conducted to determine the nutritive value of palm oil sludge fermented with A. niger added with different sources of phosphorus (NPK, P2O5, NaH2PO4). The experiment was assigned in a factorial (3x3) design. The main factor was sources of phosphorus, while the sub factor was time of incubation (0, 4 days aerobic incubation, and 4 days aerobic incubation followed by 2 days anaerobic incubation). Parameters measured were pH, soluble nitrogen, true protein and crude protein, total α-amino acid (TAAA), soluble phosphorus and total phosphor, in vitro dry matter (DCBK), and true protein digestibilities (DCP). Results from the analyses showed that fermentation increased the contents of soluble and total P, protein and TAAA and the value of in vitro protein digestibility. Fermented product added with P2O5 had the highest in vitro dry matter digestibility, while the one added with NPK had true protein content and digestibility. It was concluded that additional NPK gave the best result interm of nutritive value. Key words: Palm oil sludge, A. niger, phosphorus sources

Performances of broiler supplemented with antibiotic and Morinda citrifolia waste as sources of bioactive compounds I.A.K Bintang; A.P Sinurat; T Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the use of Zinc bacitracin antibiotic (ZnB) and dried Morinda citrifolia waste as feed additive in broiler ration was conducted. Two hundred and sixteen day old chicks (DOC) was allocated into 4 treatments: control; control + 50 ppm ZnB, and control + M. citrifolia waste at 2 levels (5 and 10 g/kg ration) with 9 replications. Each replicatation consist of 6 birds. The treatments were allocated in a completely randomized design. Variables measured were feed intake, live weight, feed conversion ratio (FCR), percentages of carcass and internal organs (liver, gizard, abdominal fat, and thickness of intestine). The results showed that feed intake of M. citrifolia waste was significantly (P<0.05) lower than that of control. Live weight and live weight gain of M. citrifolia waste treatments did not significantly different (P>0.05) from that of control. The use of M. citrifolia waste (5 and 10 g/kg) gave significantly lower (P<0.05) live weight than the use of antibiotic. FCR of M. citrifolia waste 5 g/kg ration and antibiotic was not significantly different (P>0.05), but was significantly lower (P<0.05 ) than that of control and M. citrifolia waste 10 g/kg. Carcass and internal organ percentages were not effected by antibiotic and M. citrifolia waste. Liver percentages with M. citrifolia waste 10 g/kg was significantly (P<0.05) higher and abdominal fat was lower (P>0,05) than that of control, control + antibiotic ZnB and M. citrifolia waste 5g/kg. It is concluded that the supplementation of M. citrifolia waste (5 g/kg) could substitute ZnB to improve feed eficiency in broiler ration. Key Words: M. Citrifolia Waste, Performances, Broiler

Nutritive value of palm oil sludge fermented with Aspergillus niger after therma1 drying process T Purwadaria; A.P Sinurat; Supriyati .; H Hamid; I.A.K Bintang
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 4 (1999): DECEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Solid substrate fermentation by Aspergillus niger has been carried out to improve the nutritive value of palm oil sludge (POS). POS was fermented aerobically for four days in a fermentor chambers (28°C, RH 80%), with 60% moisture content Some of the product was further incubated anaerobically for 2 days at 28°C. Both products from aerobic and anaerobic fermentation processes were dried by various methods, i.e. sunlight, oven at 60°C, oven with blower at 40°C, at the moisture content less than 11%. Results of the drying methods were also compared with the fresh fermented product. Statistic analysis using factorial design (2 x 4) showed that there was no interaction between kind of fermentation processes (aerobic and anaerobic) and drying methods (fresh, sunlight, oven 60°C, and blower 40°C) for almost all parameters except total a-amino acid content Significant results (p<0.05) were obtained on the drying methods for parameters of crude protein, true protein, in vitro dry matter and protein digestibilities, and mannanase and cellulase activities. There were no significant results between treatments in the crude fiber analysis and soluble nitrogen content Significant results also did not occur between treatment of aerob and anaerob fermentation processes for almost all parameters except for dry matter digestibilities. Results from true protein and in vitro digestibilities show that the fresh fermented product has the best nutritive value, while product dried by sunlight was best among other drying processes. Results from in vivo of protein and energy digestibilities show that there were better metabolizable energy and protein for product with aerobic process and dried with oven and blower treatments, while sunlight drying was best for product processed in anaerobic condition. Although fresh fermented product gave better result from in vitro digestibilities and enzyme activity analyses, for some reasons (easy handling and preservation) sunlight drying gave best results for products processed under anaerobic condition, especially when sunlight drying is cheap. Key words: Palm oil sludge, fermentation product, Aspergillus niger, thermal drying

Responses of broilers to Aloe vera bioactives as feed additive: The effect of different forms and levels of bioactives on performances of broilers A.P Sinurat; T Purwadaria; M.H Togatorop; T Pasaribu; I.A.K Bintang; S Sitompul; J Rosida
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Feed additives are commonly used in poultry feed as growth promotors or to improve feed efficiency. Previous results showed that Aloe vera bioactives could improve feed efficiency in broilers. Therefore, a further study was designed in order to obtain optimum doses and application methods of bioactives for broiler chickens. Aloe vera was prepared in different forms (fresh gel, dry gel, fresh whole leaf or dry whole leaf). The aloe was supplemented into the feed with concentrations of 0.25; 0.5 and 1 g/kg (equal to dry gel). Standard diets with or without antibiotics were also included as control. The diets were fed to broilers from day old to 5 weeks and the performances were observed. Results showed that the aloe-bioactives did not significantly (P>0.05) affect final body weight of broilers as compared with the control. Supplementation of 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel significantly improved feed convertion by 4.7; 4.8 and 8.2%, respectively as compared with the control. This improvement was a result of reduction in feed intake or dry matter intake without reducing the weight gain. However, supplementation of whole aloe leafs could not improve feed convertion in boilers. It is concluded that the bioactives of Aloe vera could be used as feed supplement to improve feed efficiency in broilers with no deleterious effect on weight gain, carcass yield, abdominal fat levels and internal organs. The effective concentrations of aloe gell as a feed supplement based on dry matter convertion were from 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel. Key words: Broilers, feed efficiency, feed additives, Aloe vera

Utilization of palm oil sludge in poultry diet: 2. Dried palm oil sludge and its fermented product for growing drakes Arnold P Sinurat; I.A.K Bintang; T Purwadaria; T Pasaribu
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Inclusion limit of palm oil sludge (POS) in poultry diet varies according to processing, and species or strain of the animal. Therefore, an experiment was conducted to study the utilization of fermented (FPOS) and non-fermented palm oil sludge (POS) for growing drakes. A number of 224 one week-old male ducklings were randomly distributed into 7 dietary treatments, with 4 replicates and 8 ducklings for each replicate. The dietary treatment consists of 2 kinds of feedstuffs (POS and FPOS) with 3 levels (5, 10, and 15%) and one control diet consisting neither POS nor FPOS. All diets were formulated with similar nutrient contents and meet the requirement of growing duckling and fed to 8 weeks old. The results showed that at the first week of the trial, feeding of POS or FPOS significantly (P<0.05) depressed growth of the ducklings, although the feed consumption was higher than the control. However, overall performances (body weight, feed consumption and feed conversion) of the ducklings, carcass yield, liver weight, gizzard weight and abdominal fat weight measured at the end of the trial were not significantly (P>0.05) affected by the dietary treatments. Therefore, it is concluded that it is safe to include POS or FPOS up to 15% in the diet of growing ducklings. Key words: Palm oil sludge, fermentation, ducks, growth

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