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All Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology
Retno Sri Wahjuni
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Agriculture, Biological Sciences & Forestry Immunology & microbiology Public Health Veterinary

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Detection of Mycotoxigenic Fungi on Food from Markets with the Polymerase Chain Reaction Method for Halal and Thoyiban Food Mohammad Sukmanadi; Retno Sri Wahjuni; Kadek Rachmawati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14813

Abstract

Secondary metabolites of filamentous mold are mycotoxins, which in some situations can develop on foodsderived from plants or from animals. Fusarium, Aspergillus, and Penicillium are the most common types ofmold that produce mycotoxins and also often contaminate human food and animal feed. Aflatoxins includingaflatoxin B1, B2, G1 and G2 are produced by A. flavus and A. parasiticus. M1 and M2 aflatoxins arefound in dairy products. In this study, we used PCR to detect and identify mycotoxigenic fungi material infoods from traditional markets and supermarkets in Surabaya, Indonesia. Samples of chicken meat from atraditional market and from a supermarket (10 pieces each) were placed in a conical tube and crushed in PBS.The crushed samples were centrifuged and the supernatants were cultured on potato dextrose agar (PDA)media and observed using a reverse microscope. DNA was isolated from cultured samples and subjectedto PCR with primers specific for genes encoding aflatoxins. Interestingly, we revealed that the PolymeraseChain Reaction (PCR) analysis showed A. flavus and A. ochraceus were present on chicken meat sold attraditional markets and supermarkets. In sum, enhanced precautions may be needed to ensure that foods soldin traditional markets and supermarkets are free from molds that have the potential to produce mycotoxins.Further studies are needed to detect and identify the prevalence of mycotoxins in the food supply.
Anthelmintic Efficacy of Ethanol Extract of Raw Kepok Banana Peel (Musa paradisiaca L.) Against Ascaridia galli Worms In Vitro Annastasya Yuliani Rahayu; Retno Sri Wahjuni; Dewa Ketut Meles; Retno Wulansari
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.632 KB) | DOI: 10.20473/jops.v4i2.29154

Abstract

The aims of this study was to know anthelmintic efficacy through determine the concentration, duration, and interaction between concentration and duration of raw kepok banana peel ethanol extract against mortality rate of Ascaridia galli using in vitro method. Present study was used completely randomized design. This method of this study was used a total of 600 samples of Ascaridia galli with length of 7-11 cm without differentiating their sex. The concentration of raw kepok banana peel ethanol extract was divided into 25%, 50% and 75%. Physiological saline solution and piperazine citrate 10 mg/ml were being used as negative and positive control. Each treatment then being replicated six times. The recording of mortality rate was done at 2, 4, 6, 8 and 10 hours of observation. Ascaridia galli was confirmed death if there was no movement while triggered by anatomy tweezers and dipped in slightly warm water (50 0C). The data obtained was analyzed using ANOVA factorial and continued with Duncan Multiple Range Test by SPSS 26 for Windows. The results were showed that in 75% concentration and 10 hours of treatment can cause the highest mortality rate against Ascaridia galli. Interaction between concentration and duration of treatment reveals that 75% concentration of raw kepok banana peel ethanol extract in 10 hours of observation was recorded causing the highest mortality rate against Ascaridia galli.
Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature Retta, Ayun Tria Marga; Suherni Susilowati; Sri Pantja Madyawati; Tatik Hernawati; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.49-53

Abstract

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.
Co-Authors Annastasya Yuliani Rahayu Kadek Rachmawati Meles, Dewa Ketut Mohammad Sukmanadi Retno Wulansari Retta, Ayun Tria Marga Sri Pantja Madyawati Suherni Susilowati Tatik Hernawati Wurlina, W