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All Journal Indian Journal of Forensic Medicine & Toxicology Jurnal Agro Veteriner
Mohammad Sukmanadi
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Public Health Veterinary

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Detection of Mycotoxigenic Fungi on Food from Markets with the Polymerase Chain Reaction Method for Halal and Thoyiban Food Mohammad Sukmanadi; Retno Sri Wahjuni; Kadek Rachmawati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14813

Abstract

Secondary metabolites of filamentous mold are mycotoxins, which in some situations can develop on foodsderived from plants or from animals. Fusarium, Aspergillus, and Penicillium are the most common types ofmold that produce mycotoxins and also often contaminate human food and animal feed. Aflatoxins includingaflatoxin B1, B2, G1 and G2 are produced by A. flavus and A. parasiticus. M1 and M2 aflatoxins arefound in dairy products. In this study, we used PCR to detect and identify mycotoxigenic fungi material infoods from traditional markets and supermarkets in Surabaya, Indonesia. Samples of chicken meat from atraditional market and from a supermarket (10 pieces each) were placed in a conical tube and crushed in PBS.The crushed samples were centrifuged and the supernatants were cultured on potato dextrose agar (PDA)media and observed using a reverse microscope. DNA was isolated from cultured samples and subjectedto PCR with primers specific for genes encoding aflatoxins. Interestingly, we revealed that the PolymeraseChain Reaction (PCR) analysis showed A. flavus and A. ochraceus were present on chicken meat sold attraditional markets and supermarkets. In sum, enhanced precautions may be needed to ensure that foods soldin traditional markets and supermarkets are free from molds that have the potential to produce mycotoxins.Further studies are needed to detect and identify the prevalence of mycotoxins in the food supply.
Analysis of Capsicum annuum L. Methanolic Extract and Its Potential as a Hepatoprotector Mustofa Helmi Effendi; Mohammad Sukmanadi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15869

Abstract

ThinLayerChromatography(TLC) method performed to purify capsaicin compound in C. annum L. and test the bioactive component of Capsicum Annum L on MAPK1 target proteins using the Immunohistochemistry (IHC) method to determine the reactions between receptor-ligand (antigen-antibody) complexes formed and become a reference in vivo tests. Natural ingredients need to be extracted to get some or all of the active ingredients used to synthesize the right dosage, easily stored, and maintain for a long time. The active ingredient of capsaicin is efficacious as a stimulant of gastric acid secretion and prevents infections in the digestive system.The immunohistochemical examination is intended to determine the expression of MAPK1 (ERK) on cells of mice hepatocytes (Mus musculus). MAPK 1 (ERK) expression score data were obtained using the modified Remmele method is the result of multiplying the percentage score of immunoreactive cells with the color intensity score on immunoreactive cells. The abnormal target protein signaling pathway contributes to cell proliferation, differentiation, survival, and uncontrolled cell apoptosis.Analysis of amino acid residues, Capsaicin to MAPK1haverelativelysimilarresiduessothatitcanbeconcludedthat the binding sites of the two compounds are close to similarities and affect receptors at relatively similar sites, namely oncogenicreceptors/ proteins. Concluded that the ethanol extract of Capsicum. annum L. had a greater in the powder form , TLC method showed the capsaicin standard was 1020 µg/ml, that capsaicin in chilies (Capsicum Annum L.) can synergistically inhibit cell through reactions in the anti-apoptotic MAPK 1 potentiates the antiproliferative action that can promote hepatoprotector, a finding that demands further clinicaltesting.
Potential of Liquid Probiotics Bacillus subtilis and Bacillus coagulans on Broiler Chicken Carcas Percentage and Business Analysis Muhammad Ilyas Wahyudi; Maslichah Mafruchati; Sunaryo Hadi Warsito; Mohammad Sukmanadi; Sri Hidanah; Mohammad Anam Al-Arif
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.59218

Abstract

The aim of this research was to find out liquid probiotic Bacillus subtilis and Bacillus coagulans as feed aditive to carcass percentage and economic analysis in broiler. This research used 24 broiler aged 20 day. P0 (control group) was given 2 ml of aquadest. P1, P2, and P3 were given liquid probiotic Bacillus subtilis and Bacillus coagulans with different dosage respectively (2 ml, 4 ml, and 6 ml). The sample of data was taken by weighing broiler manually. The data of carcass percentageanalyzed using one way Analysis of Variance (ANOVA) and if showed significant difference, continue by Duncan test as post-hoc test. The data of business analysis analyzed by descriptive. The result of this research was indicate that all doses 2 ml, 4 ml and 6 ml of liquid probiotic were significnat difference (p<0.05) in carcass percentage. The result of doses 4 ml of liquid probiotic was most higher than doses 2 ml and 6 ml in carcass percentage. Business analysis in this research gave effect in contribution margin and value > 1 of the revenue cost ratio which could be stated that the broiler business was feasible.
Probiotics Addition Potential to Increase Body Weight, Feed Consumption and Feed Conversion on Pre-Layer Laying Hens Sukmawati Lailatul Jannah; Mirni Lamid; Mohammad Sukmanadi; Mohammad Anam Al Arif; Sri Hidanah; Sri Chusniati
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51392

Abstract

Probiotics are all forms of microbial cell preparations or components of microbial cells that have a beneficial effect on the health and life of the host. Some feed additives such as the Antibiotic Growth Promoter (AGP) hormone have been banned. Therefore, nowadays, probiotics are often used as additives to replace antibiotics. The probiotics that are widely used are lactic acid bacteria because they can increase the efficiency of digestion and absorption of nutrients. The purpose of this study was to determine the effect of giving probiotics a combination of Bacillus coagulans and Bacillus subtilis with doses of 2 ml, 4 ml, and 6 ml/head/day orally on weight gain, feed consumption, and decreased feed conversion of laying hens. This study was conducted for 2 weeks using pre layer chickens aged 21 weeks to 23 weeks old as many as 24 chickens, randomized into 4 treatments with 6 replications with P0 as control, P1 = 2 ml, P2 = 4 ml, and P3 = 6 ml. The probiotic used is a combination of probiotics Bacillus coagulans and Bacillus subtilis with a concentration of 1x107 CFU/ml in liquid.
Histological Features of the Duodenum of Mice (Mus musculus) Treated with Liquid Probiotic Isolates of Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae Devi Sri Rohmani; Mohammad Anam Al Arif; Sri Agus Sudjarwo; Mohammad Sukmanadi; Eka Pramyrtha Hestianah; Widya Paramita Lokapirnasari
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57620

Abstract

This study aims to determine the histological description of the height and width of the duodenal villi of mice (Mus musculus) treated with liquid probiotic isolates of Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae. Histological examination of the duodenum of mice was carried out by making histological preparations from the height and width of the duodenal villi which were observed under a microscope with 100x magnification. Statistical analysis in this study used One-Way ANOVA and Duncan's test. The results showed that the control group and the treatment group had significantly different height and width of the duodenal villi (p<0.05). The conclusion of this study is that the administration of isolates Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae can increase the height and width of the duodenal villi of mice and the P2 group with a dose of 0.27 mL/kg BW has the greatest average value for the height and width of the duodenal villi with an average height of 360.80 μm and an average width of 152.30 μm.
Survey of types and use of traditional medicinal plants for beef cattle in Bontang City Amar Ma'ruf Fachrul Mu'minin; Lilik Maslachah; Sri Hidanah; Ratna Damayanti; Kadek Rachmawati; Mohammad Sukmanadi
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64448

Abstract

Bontang city is suitable for research due to the limited data on the use of traditional herbal medicine in beef cattle. This study aims to survey the types and usage of traditional herbal plants in beef cattle in Bontang city, providing new insights for local farmers. Data collection involved observation, recording, and photographic documentation. Samples were taken from Bontang city, including West Bontang, South Bontang, and North Bontang districts. Traditional medicinal plants such as garlic, brotowali, coconut, turmeric, temulawak, guava, and lime thrive in these conditions. The majority of respondents are beef cattle farmers aged 51-60 years with a low level of education. Experience in cattle farming for more than 4 years has improved their skills and productivity. The primary source of information on herbal plants is passed down through generations from their parents. The results of the survey on the types and usage of traditional herbal plants in beef cattle in Bontang city can be summarized as follows. Seven plant species are used by beef cattle farmers as natural remedies, namely garlic, brotowali, coconut, turmeric, temulawak, guava, and lime. 
Co-Authors Al arif, Mohammad Anam Amar Ma'ruf Fachrul Mu'minin Chusniati, Sri Devi Sri Rohmani Eka Pramyrtha Hestianah Kadek Rachmawati Lilik Maslachah Mafruchati, Maslichah Mirni Lamid Muhammad Ilyas Wahyudi Mustofa Helmi Effendi Ratna Damayanti Retno Sri Wahjuni Sri Agus Sudjarwo Sri Hidanah Sukmawati Lailatul Jannah Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari