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All Journal Indian Journal of Forensic Medicine & Toxicology Journal of International Surgery and Clinical Medicine Indonesian Journal of Biomedicine and Clinical Sciences
Romaniyanto
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Role of Preconditioning Adipose Mesenchymal Stem Cells in Regeneration Disk: a Review Article Romaniyanto; Hari Basuki Notobroto; Cita Rosita Sigit Prakoeswa; Fedik Abdul Rantam; Dwikora Novembri Utomo; Heri Suroto; Ferdiansyah
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14892

Abstract

Intervertebral disk (IVD) degeneration (IDD) is a degenerative disease and often found in daily practice.The managements that were used so far are only symptomatic and have not been able to solve the diseasecompletely. The use of mesenchymal stem cells (MSCs) has been widely tested as a solution for theregeneration of IVD in IDD. Adipose-derived mesenchymal stem cells (ASCs) are one of the sources ofMSCs which were widely studied as an alternative. One of the factors that can influence the efficiency andcapacity of ASCs in IDD cases is preconditioning. Generally, there were three pre-conditioning strategies ofASCs before being used as therapy, (1) modifying the environment, (2) using Growth Factors, Cytokines,Chemokines, Hormones, (3) using pharmacological and chemical agents. Preconditioning on ASCs canchange its function in various ways. We tried to link all these things together so that we could know the roleof pre-conditioning ASCs in the regeneration disk.
Bovine nucleus pulposus decellularization using freeze drying technique to form a biological scaffold Romaniyanto; Prijosedjati, Raden Andhi; Ermawan, Rieva; Hakim, Fikar Arsyad; Saddalqous
Journal of International Surgery and Clinical Medicine Vol. 4 No. 1 (2024): (Available online: 1 June 2024)
Publisher : Surgical Residency Program Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jiscm.v4i1.54

Abstract

Introduction: As much as 40% of low back pain patients are caused by degenerative disc disease. The current treatments are fusion stabilization and total disc replacement, which have a risk for adjacent segment disease. These suboptimal results have become the beginning of the development of regenerative therapy. The success of this therapy will increase if the scaffold meets the ideal conditions. Biological scaffolds provide a cell growth environment that resembles the original tissue. Bovine intervertebral nucleus pulposus can become promising scaffolds. Objective: This research describes the proper freeze-drying approach for decellularizing bovine nucleus pulposus to create a biological scaffold. Methods: We conducted an experimental post-test control design study using bovine coccygeal nucleus pulposus material. All treatment groups underwent freeze-drying with Buchi Lyovapor™ L-200/L-200 Pro. All groups were evaluated for the level of decellularization using the Quick-DNA™ Miniprep Plus Kit (Zymo Research®) and remaining glycosaminoglycan levels by Alcian Blue staining. Results: Comparison of DNA concentrations obtained p-values respectively <0.0001 (p <0.05), which means that all the treatments showed a decrease in DNA concentration compared to the control group. The comparison of the glycosaminoglycan percentage between the P1 vs. Control group obtained a value of p=0.381 (p>0.05), which means that the glycosaminoglycan percentage results for the P1 group were not significantly different from the control group. Conclusion: This study of group P1 showed that decellularization of the bovine nucleus pulposus by freeze-drying technique can form an excellent biological scaffold.
Umbilical cord mesenchymal stem cells and their secretome in peripheral nerve regeneration: a narrative review promising therapeutic strategy Sumarwoto, Tito; Hadinoto, Seti Aji; Romaniyanto; Rhatomy, Sholahudin; Utomo, Pamudji; Idulhaq, Mujaddid; Santoso, Asep; Suroto, Heri
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 4 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i4.24055

Abstract

The injuries of peripheral nerves remain a substantial clinical challenge because of their limited regenerative capacity, the complexity of nerve repair, and limitations of current treatment strategies. Umbilical cord mesenchymal stem cells (UC-MSCs) and their secretome have shown promise as regenerative treatments due to their distinct biological characteristics. This review explores the possibility of UC-MSCs and their secretome in promoting peripheral nerve regeneration, by their action mechanisms, therapeutic applications, and current preclinical - clinical evidence. UC-MSCs have proven to be capable of support Wallerian degeneration, improving axonal growth, reducing inflammation, and improving functional recovery in both animal models and early clinical studies. Their secretome has been shown to promote neuroprotection and functional repair, avoiding the risks of receiving a direct stem cell transplant. Challenges remain in standardizing UC-MSC-based therapies, ensuring long-term safety, and enhancing delivery strategies. Further clinical trials are needed to determine the efficacy, safety, and scalability of UC-MSC therapies for widespread clinical use. UC-MSCs and their secretome provide a unique, cell-free and cell-based strategy to peripheral nerve regeneration. Future advancements in biomaterial integration, gene editing, and personalized medicine will be essential to implementing these treatments in clinical settings.
Co-Authors Cita Rosita Sigit Prakoeswa Dwikora Novembri Utomo Ermawan, Rieva Fedik Abdul Rantam Ferdiansyah Hadinoto, Seti Aji Hakim, Fikar Arsyad Hari Basuki Notobroto Heri Suroto Idulhaq, Mujaddid Prijosedjati, Raden Andhi Rhatomy, Sholahudin Saddalqous Santoso, Asep Sumarwoto, Tito Utomo, Pamudji