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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Harpodon Borneo Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Borneo Saintek Journal of Fisheries & Marine
Burhanuddin Ihsan
Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Environmental Science Law, Crime, Criminology & Criminal Justice Nursing Social Sciences

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ISOLASI DAN IDENTIFIKASI BAKTERI Vibrio sp. PADA KERANG KAPAH (Meretrix meretrix) DI KABUPATEN TRENGGALEK Burhanuddin Ihsan; Endah Retnaningrum
Jurnal Harpodon Borneo Vol 10, No 1 (2017): Volume 10 No. 1 April 2017
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v10i1.196

Abstract

Meretrix meretrix is one type of shellfish that are included in the bivalves that are filter feeders that live in the waters. Many bacteria that live in the estuary with high salinity in the range of 20-40 ppt one of which is the Vibrio bacterium. This research aims to determine the presence of Vibrio spp. bacteria in M. meretrix from Cengkrong Mangrove Eduwisata River, Trenggalek. The Isolation of Vibrio bacteria was conducted by streat plate method on selective media of TCBSA (Thiosulfate Citrate Bile Salt Sucrose Agar) and identify the Vibrio bacterium through the morphological and biochemical tests based Bergey's Manual of Systematic Bacteriology In Microbiology laboratory of Biology Faculty of Gadjah Mada University. The Result showed there are 7 isolates Vibrio spp. bacterias in M. meretrix they are isolate 1.1, 1.4, 1.7, 1.8. 5.3, 3.5, 2.6 PSBI Keyword: Meretrix meretrix, Vibrio, identification
Identifikasi Bakteri Patogen (Vibrio spp. dan Salmonella spp.) yang Mengontaminasi Ikan Layang dan Bandeng di Pasar Tradisional: Identification of Pathogenic Bacteria Contamination (Vibrio spp. and Salmonella spp.) in Flying Fish and Milkfish in Traditional Markets Burhanuddin Ihsan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 24 No 1 (2021): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v24i1.34198

Abstract

Sumber konsumsi ikan masyarakat Kota Tarakan adalah ikan layang dan bandeng yang berasal dari pasar tradisional. Pengelolaan pasar tradisional yang ada di Kota Tarakan cukup memadai namun, tingkat kesehatan dan higienis lingkungan masih kurang baik, penanganan yang belum memadai memungkinkan banyak bakteri yang berkembang dan mengontaminasi ikan. Tujuan penelitian untuk mengetahui kontaminasi bakteri patogen (Vibrio spp. dan Salmonella spp.) pada ikan yang dijual di pasar tradisional kota Tarakan. Penelitian dilakukan dengan metode deskriptif dengan beberapa tahapan mulai dari observasi pasar, pengambilan sampel, isolasi bakteri dan identifikasi. Isolasi bakteri Vibrio menggunakan media selektif TCBS (Thiosulfate Citrate Bile Salt Sucrose) dan bakteri Salmonella menggunakan media SSA (Salmonella Shigella Agar) identifikasi bakteri Vibrio dan Salmonella yang berpedoman pada buku Bergey’s Manual of Determinative Bacteriology berdasarkan sifat morfologi dan kimiawi. Hasil identifikasi terdapat kontaminasi bakteri Vibrio spp. dan Salmonella spp. pada hasil perikanan yang dijual di pasar tradisional kota Tarakan khususnya ikan bandeng dan ikan layang.
DETEKSI DAN IDENTIFIKASI BAKTERI Salmonella spp. PADA IKAN BANDENG YANG DIJUAL DI PASAR GUSHER KOTA TARAKAN Burhanuddin Ihsan; Ira Maya Abdiani; imra imra
Jurnal Harpodon Borneo Vol 11, No 1 (2018): Volume 11 No.1 April 2018
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v11i1.538

Abstract

Keberadaan bakteri Salmonella spp pada ikan bandeng dapat mempengaruhi aspek kualitas, kemunduran mutu produk perikanan dan keamanan dalam mengkonsumsi. Tujuan penelitian untuk mengetahui tingkat kesegeran dan kontaminan bakteri pada ikan bandeng yang dijual di Pasar Guser Kota Tarakan dan mendeteksi keberadaan bakteri patogen (Salmonella spp.). Sampel daging ikan dihaluskan sebanyak 25 gram dan dimasukan ke dalam Erlenmeyer berisi 225 ml Alkhali Pepton Wather (APW) kemudian di homogenkan dan diinkubasi selama 5-8 jam. Isolasi bakteri Salmonella spp. dengan menggunakan media Salmonella Shigella Agar (SSA) kemudian diinkubasi selama 24-48 jam suhu 37oC dan diidentifikasi dengan uji biokimia dengan menggunakan Bergey’s Manual of Determinative Bacteriology. Hasil penelitian diperoleh bahwa mutu dan kualitas ikan bandeng yang dijual di Pasar Gusher Kota Tarakan sangat baik dan segar dengan tingkat kontaminan bakteri dibawah ambang batas/baku mutu SNI yaitu 4x104 koloni/gram. Serta terdeteksi mengandung bakteri patogen (Salmonella spp.).
KEANEKARAGAMAN BAKTERI PADA PERAIRAN DI KAWASAN KONSERVASI MANGROVE DAN BEKANTAN (KKMB) KOTA TARAKAN Yulma Yulma; Burhanuddin Ihsan; Anisah Rafikah
Jurnal Borneo Saintek Vol 1, No 3 (2018)
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/borneo_saintek.v1i3.935

Abstract

Bakteri di perairan merupakan komponen biotik yang penting pada proses dekomposisi sebagai sumber penyedia zat hara. Tujuan penelitian ini adalah untuk mengetahui keanekaragaman bakteri pada perairan di Kawasan Konservasi Mangrove dan Bekantan (KKMB) Kota Tarakan. Penelitian ini dilaksanakan pada bulan Maret sampai Mei 2018 dengan menggunakan metode deskriptif dengan melakukan identifikasi bakteri pada perairan melalui beberapa tahapan pengujian yaitu uji gram, uji utama dan uji lanjut yang dilakukan di laboraturium Stasiun Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan kelas II Tarakan. Hasil penelitian menunjukkan keanekaragaman bakteri pada perairan sebanyak 9 bakteri (Genus) yaitu Bacillus spp., Corynebacterium spp., Listeria spp., Enterobacteria spp., Pseudomonas spp., Aeromonas spp., Micrococcus spp.,  Staphylococcus spp., dan Actinobacillus spp., dan yang paling dominan ditemukan adalah Bacillus spp.
Pengenalan Budi Daya Ikan dalam Ember untuk Kemandirian Pangan di Kampung Enam, Kota Tarakan Miska Sanda Lembang; Rukisah Rukisah; Jimmy Cahyadi; Heppi Iromo; Azis Azis; Nuril Farizah; Muhammad Amien; Diana Maulianawati; Zainuddin Zainuddin; Awaludin Awaludin; Burhanuddin Ihsan; Ricky Febrinaldy; Kartina Kartina; Santria Santria; Patrice Sevania
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 3 (2022): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.8.3.262-268

Abstract

The Covid-19 pandemic has had a major impact on society, such as job losses, rising staples, and economic hardship. Prices of food needs that continue to increase and are not affordable have resulted in food problems, one of which is protein. One of the high-quality protein is protein from fish. Fish as a fishery commodity that is relatively easy to cultivate on a large industrial scale and household scale for food self-sufficiency is catfish (Clarias sp). The purpose of this activity is to add insight and knowledge of the community about budikdamber for food self-sufficiency. The method of implementing community service activities in Kampung Six is carried out by counseling and training methods on Budikdamber. In this training activity, a bucket with 40 liters of water and 20 catfish seeds was used. Maintenance was carried out for 60 days with ad satiation feeding three times a day. Observations were made on growth, survival and water quality. The results of the activity found that 93% of the community partners had understood about Budikdamber. Budikdamber maintenance by partner communities gets an absolute weight growth value of catfish of 14 g, catfish survival value of 75%, and 1 bunch of kale per bucket. Based on the results of this activity, it is hoped that there will be the provision of special land for Budikdamber as an example for people who want to develop in their respective yards.
The Numerical Phenetic of Taxonomy Vibrio in Shellfish (Meretrix meretrix) at Edu-Tourism Mangrove Cengkrong Beach Trenggalek Burhanuddin Ihsan; Endah Retnaningrum
Jurnal Ilmiah Perikanan dan Kelautan Vol. 12 No. 2 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v12i2.17846

Abstract

HighlightIdentify the Vibrio bacteriaDiversity of shellfish Vibrio bacteria (Meretrix Meretrix)Relationship similarity of Vibrio bacteria with MVSP 3.1 Program produces DendogramAbstract Meretrix meretrix is one type of shellfish that is potential, and economic value, which high animal protein content as well as seafood that is widely consumed in Indonesia. This study aims to determine the diversity and similarity of Vibrio in M. meretrix at eduwisata mangrove Cengkrong Beach, Trenggalek. Isolation and identification of Vibrio was conducted by using the media TCBSA (Thiosulfate Citrate Bile Salt Sucrose Agar) streat plate through morphological observation, physiological and biochemical tests based Bergey's Manual of Systematic Bacteriology conducted in the laboratory of Microbiology Faculty of Biology, Universitas Gajah Mada. Data analysis was performed using the MVSP (Multivariate Statistical Package) 3.1 in the form of a dendrogram showing the similarity relationship Vibrio bacteria. The results of the identification of Vibrio bacteria in M. Meretrix are V. herveyi, V. parahemolyticus, V. nereis and V. proteolyticus. V. herveyi has similarities with V. parahemolyticus 66% and 62% with V. proteolyticus while with V. nereis bacterium 54%.
Co-Authors Anisah Rafikah Awaludin, Awaludin Azis - Hamzah cahyadi, jimmy Endah Retnaningrum Endah Retnaningrum Heppi Iromo imra imra Ira Maya Abdiani Kartina Kartina Maulianawati, Diana Miska Sanda Lembang Muhammad Amien Nuril Farizah Patrice Sevania Ricky Febrinaldy Rukisah, Rukisah Santria Santria Yulma Yulma Zainuddin Zainuddin