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All Journal Jurnal Natur Indonesia JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Planta Tropika Informatika Pertanian Jurnal Entomologi Indonesia
Kurniawan Budiarto
Indonesian Ornamental Crops Research Institute (IOCRI), Jl. Raya Ciherang, Segunung Pacet Cianjur 43253, West Java, Indonesia Phone (62-263) 512607
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Environmental Science Immunology & microbiology Veterinary

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Elimination Of CVB (Chrysanthemum Virus B) From A Range Of ChrysanthemumTHE Varieties By Meristem Culture Following Antiviral and Heat Treatments Budiarto, Kurniawan; Budi, Budi Marwoto; Lia, Lia Sanjaya; Muchdar, Muchdar Soedarjo; Indijarto, Indijarto Budi Rahardjo
BIOTROPIA Vol. 18 No. 2 (2011): BIOTROPIA Vol. 18 No. 2 December 2011
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.283 KB) | DOI: 10.11598/btb.2011.18.2.45

Abstract

CVB elimination for retaining healthy protocols from infected chrysanthemum plant was investigated trough combined treatment of meristem culture with synthetic antiviral ribavirin and thermotherapy under in vitro conditions. The biological material used on the experimentation was constituted of six commercial varieties: Dewi Sartika, Saraswati, Yellow Fiji, White Puma, Yellow Puma and White Reagent.  Tissue culture initiation was conducted through plantlet establishment using MS supplemented with IAA.  Ribavirin was added in media treatments with the concentration of 40 mg/l.  Parallel with this step, heat treatment with different durations (1, 2, and 3 weeks) was also conducted on the plantlets.  Meristem culture was done following the chemo- and thermotherapy.  The experiment resumed the failure single treatment of meristem culture in eliminating CVB from the infected chrysanthemum plantlets.  Under heat treatment, percentage of virus-free plantlets increased along with the duration of thermotherapy, though the survival rate of plantlets decreased in lengthened heat treatment. The best results regarding virus free plant percentage was obtained when meristem culture was applied following ribavirin or three weeks heat treatment which effectively eliminated virus particles from the plant.
Identification of Ralstonia solanacearum Isolated from a New Host : Cosmos caudatus in Indonesia Hanudin, Hanudin; Budiarto, Kurniawan; Marwoto, Budi
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18795.891 KB) | DOI: 10.11598/btb.2014.21.1.340

Abstract

The evolutionary dynamic of pathogenic microorganism might change pathogen-host interaction including the establishment of pathogen colonization in a new host.  The information of the new host of devastating pathogens will serve a basis for their control method.  An unknown bacterial wilt disease attacking wild cosmos (Cosmos caudatus) crop in Pacet-Cianjur, Indonesia was suspiciously caused a novel strain of Ralstonia  solanacearum.  The research was carried out to investigate the causal agent, characteristics and its grouping based on the morphological, physiological and biochemical properties and the relationship of the pathogen with the hosts.  The results showed the incidence of bacterial wilt on wild cosmos was epidemiologically caused by the species complex of pathogen R. solanacearum.  Based on the symptoms, morphological, physiological, biochemical and pathogenicity evaluations, the strain classified into group 7, race 1 and biovar 3 and group 7.  This was the first report of the incidence of R. solanacearum hosted naturally in wild cosmos in Indonesia.
Co-Authors Ahmad Syahrian Siregar, Ahmad Syahrian Arsanti, Idha W Budi Marwoto Budi, Budi Marwoto Dhani Gartina Elok Rifqi Firdiana, Elok Rifqi Evi Silvia Hanudin Hanudin Indijarto Budi Rahardjo, Indijarto Budi Indijarto, Indijarto Budi Rahardjo Izmi Yulianah L. Rosario, Teresita Lia, Lia Sanjaya Masnar, Adley L. Mas’udah, Shofiyatul Mizu Istianto Muchdar, Muchdar Soedarjo Otto Endarto Pramurjadi, Andy Rasiska Tarigan Sasanti Widiarsih Susi Wuryantini, Susi Triasih, Unun Wakiah Nuryani wicaksono, rudi cahyo Yusuf, Hadi Mokhamad