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Hidayat Syarief
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Health Professions Industrial & Manufacturing Engineering Public Health

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Journal : Jurnal Teknologi Dan Industri Pangan

 1 
EKSTRAKSI DAN ANALISIS FITOSTEROL LEMBAGA GANDUM (Triticum sp.) [Extraction and Analysis of Phytosterol from Wheat Germ (Triticum sp.)] Sri Anna Marliyati; Hidayat Syarief; Deddy Muchtadi; Latifah K Darusman; Rimbawan Rimbawan
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 1 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Phytosterol may reduce the absorption of cholesterol, and used for preventing atherosclerosis. It is limited in soybean, but potentially abundant in wheat germ. Research on the utilization of wheat germ sterol had not been reported so far. Many aspects of germ sterol extraction from wheat germ and its characteristics were still unknown. In this research, the best extraction method, kinds and content of phytosterol from wheat germ were investigated. This research consisted of two steps: (1) extraction of phytosterol directly form whole germ and ground germ using hexane, and indirect extraction through germ oil using hexane and mixed solvent of hexane and ethanol, and direct extraction from ground germ using ethanol; (2) analysis of the type and content of phytosterol in the crude extract through the following steps: preparation of crude extract, fractionation, and analysis. Results showed that indirect extraction through germ oil was considered as the best method which yielded 1.37% of phytosterol. The highest yield was obtained when extracted using a mixed solvent of hexane – ethanol 82:18. However, the odor of ethanol and hexane (gasoline like odor) was still detected. The solvent’s ratio of hexane to ethanol at 1:2 resulted better odor of the extract. Extraction of sterol using ethanol yielded 18.39% of sterol when the ratio of germ to ethanol at 1:10 (w/v) was applied. Results of quantitative analysis on the main component of crude extract of wheat germ sterol showed that the total content of sterol extracted with mixed solvent was higher than those extracted with ethanol. The ratio of hexane to ethanol at 1:1 (v/v) gave higher content of total sterol, stigmasterol and campesterol, whereas higher content of -sitosterol was produced at the solvent’s ratio of hexane to ethanol at 1:2 (v/v).
In Vitro Antioxidant Activity of Stabilized Rice Bran and Its Fraction Evy Damayanti; Fransiska Rungkat Zakaria; Hidayat Syarief; C Hanny Wijaya; Djoko Said Damardjati
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Some Researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in the prevention of low density lipoprotein (LDL) and human lymphocyte from oxidation under oxidative stress was still limited. The objective of this study was the investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LCL and lymphocyte from oxidation. Human plasma were supplemented with the samples of : rice bran oil (RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308.3, 22.2, 5.2, 10.4, and 10.4 µg/ml, respectively. Afterward, the human LDL were collected by ultracentrifuge and diluted until a concentration of 200 µg protein/ml was reached. Human LDL isolates were then oxidized with CuSO4 5 µM for measuring antioxidant activity of the sample. The length of incubation, H2O2 concentration, period of sample supplemented into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured. The samples used in the lymphocyte were RBO IR-64, unsaponifiable matter IR-64, and oryzanol standard at the concentrations of 133.2 – 2, 132.0 µg/ml, 9.6 – 153.6 µg/ml, and 2.4 – 37.7 µg/ml, consecutively. The result showed that malonaldehyde concentration in human LDL decreased significantly (α = 0.05), 15 – 41% and 39 – 56% compared to the control. The absorbance of living lymphocyte cell in culture was not influenced by the type and concentration of RBO and its fraction. The addition of hydrogen peroxide (H2O2) 3 mM into culture sifnificantly lowered the absorbance as compared to culture without (H2O2). Key words :Oryzanol, oxidative stress, LDL-oxidized, lymphocyte and antioxidant activity.
SUPLEMENTASI STEROL LEMBAGA GANDUM (Triticum sp.) PADA MARGARIN (Supplementation of Margarine with Wheat Germ Sterol) Sri Anna Marliyati; Hidayat Syarief; Deddy Muchtadi; Latifah K. Darusman; Rimbawan .
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Margarine is a water in oil (w/o) emulsion product which is widely used for household cooking and baking industry. Consuming of margarine, which contains trans fatty acid may cause health problem due to the increase of LDL cholesterol. Since margarine is also a good carrier of phytosterol which prevent the absorption of cholesterol, there is a possibility to formulate a healthier margarine. In this research formulation and characteristics of products was investigated. The research work consisted of two steps: (1) supplementation of wheat germ sterol into margarine (two methods) and  (2) analysis of physical, chemical characteristics and hedonic score. Parameters of physical characteristics were melting point and emulsion stability, whereas chemical characteristics were water and oil contents. The hedonic test was carried out based on product’s color, odor, taste, texture, and spreadability. Results showed that method II of supplementation produced better margarine than method I, in which the concentration of sterol in the margarine was higher with a melting point similar to that of control, better emulsion stability, and higher hedonic score. Supplementation process was carried out by mixing sterol into fat phase melted at 50 0C, followed by mixing with aqueous phase at 4 0C. Sterol used for method II was extracted using mixed solvent of hexane and ethanol at the ratio of 1:2 (v/v), which was resulted from previous experimentation.
Co-Authors Amini Nasoetion ASEP SAEFUDDIN Bernatal Saragih C Hanny Wijaya Clara M Kusharto Clara M. Kusharto Dadang Sukandar Deddy Muchtadi Diah Krisnatuti Djoko Said Damardjati Endang Rohmawati Erli Mutiara Euis Sunarti Evy Damayanti FRANSISKA RUNGKAT ZAKARIA Hadi Riyadi Hardiansyah . Hardinsyah Husaini . Ikeu Tanziha Latifah K Darusman Latifah K. Darusman Mewa Ariani Muhamad Rizal Martua Damanik Ratna Megawangi Rimbawan , Soekirman Soekirman Sri Anna Marliyati Tetty Herta Doloksaribu Tiurma Sinaga