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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Degraded and Mining Lands Management Jurnal Biologi Tropis JURNAL PENGABDI Spizaetus: Jurnal Biologi dan Pendidikan Biologi Indigenous Biologi Indonesian Journal of Applied Research (IJAR) Jurnal Biosense GEOGRAPHIA: Jurnal Pendidikan dan Penelitian Geografi Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa (Journal of Environmental Sustainability Management) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Chatarina Gradict Semiun
Universitas Katolik Widya Mandira
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Sebaran Vegetasi Bunga Edelweis di Kawasan Taman Nasional Kelimutu dengan Citra Landsat 8 TM Siki, Theodosia Monika; Pani, Emilianus; Semiun, Chatarina Gradict
GEOGRAPHIA : Jurnal Pendidikan dan Penelitian Geografi Vol. 6 No. 2 (2025): Desember
Publisher : Jurusan Pendidikan Geografi Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/gjppg.v6i2.10450

Abstract

Edelweis Anaphalis longifolia is one of Indonesia s mountain vegetation that grows at an altitude of 1 000 2 000 meters above sea level and plays a role as a pioneer plant in volcanic soils Anaphalis longifolia is one of the places in Kelimutu National Park which spans altitudes of 1 000 1 731 meters above sea level Using Landsat satellite imagery the research aims to identify environmental factors influencing edelweis distribution Data collection was conducted across six tracking routes: Moni Wologai Protelindo Dedumudi Niowula Ratebeke and Saga Bukit Sokoria combining field observations with the analysis of Landsat 8 imagery elevation SHP rock type SHP and NDVI values The results of the study show that the distribution of edelweis is most commonly found at an altitude of 1471 1599 meters above sea level Meanwhile the distribution of edelweis is at least at an altitude of 1114 1200 meters above sea level The types of rocks in the Kelimutu National Park area are young volcanic rocks and the distribution of edelweis tends to be more abundant in areas with moderate NDVI values 0 25 0 35 Environmental factors such as temperature humidity sunlight intensity soil pH and wind speed play a role in the spread of Edelweis in Kelimutu National Park < em>< p>
Isolasi dan Uji Aktivitas Antimikrobia Bakteri Endofit Akar Mangrove Rhizophora mucronata di Kawasan Ekowisata Mangrove Kupang Alexander Tage; Chatarina Gradict Semiun; Yulita Iryani Mamulak
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.730

Abstract

Semi-arid mangrove ecosystems harbor significant potential as sources of endophytic bacteria producing adaptive antimicrobial compounds. This study aimed to isolate and evaluate the antimicrobial activity of endophytic bacteria from Rhizophora mucronata prop and lateral roots across three management zones (core, buffer, and utilization) in the Kupang Mangrove Ecotourism Area. Sampling was conducted in March–April 2026, followed by root surface sterilization and isolation on Nutrient Agar. Phenotypic characterization was performed macroscopically, microscopically, and biochemically, while antimicrobial activity was assessed using the Kirby-Bauer disk diffusion method against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. From 69 initial colonies, 18 pure isolates were obtained, with Bacillus sp. as the dominant genus (44.4%). Notably, Bacillus sp. isolates ZS-04 and ZI-05 exhibited the most potent bioactivity, yielding very strong (>20 mm) inhibition zones against S. aureus and strong (10–20 mm) inhibition against E. coli. One-way ANOVA revealed no significant zonal differences for S. aureus (p = 0.785), whereas a statistically significant zonal effect was observed for E. coli (p = 0.045), particularly between the utilization and buffer zones. These findings confirm that endophytic Bacillus sp. from R. mucronata roots, particularly isolates ZS-04 and ZI-05, produce bioactive compounds with strong, targeted antibacterial activity against Gram-positive pathogens, highlighting semi-arid mangrove endophytes as promising, stress-adapted reservoirs for novel natural antimicrobial agents.
UJI KANDUNGAN GIZI TAMBELO (Bactronophorus sp.) YANG DIBUDIDAYAKAN DI SUNGAI SIMATALU OLEH MASYARAKAT DESA SIMATALU KABUPATEN KEPULAUAN MENTAWAI PROVINSI SUMATERA BARAT Bernadus Esli Salembeheu; Leonardus Banilodu; Chatarina Gradict Semiun
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 3 No 1 (2022): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v3i1.54

Abstract

Gizi merupakan komponen penting dalam bahan pangan untuk menunjang kesehatan manusia. Salah satu bahan pangan yang dikonsumsi adalah makanan tradisional dari desa Simatalu yaitu Tambelo. Makanan khas Tambelo ini belum diketahui kandungan gizinya. Tujuan penelitian ini adalah untuk mengetahui kadungan gizi yang terdapat pada Tambelo (Bactronophorus sp.) hasil pembudidayaan masyarakat desa Simatalu. Sampel Tambelo segar diperolehdarisungaiSimatalu yang merupakanhasilbudidaya. Sampel tersebut kemudian dianalisis kandungan karbohidrat, protein, lemak, dan mineral yang dilakukan di Laboratorium Fakultas MIPA Universitas Negeri Padang.Penetapan kandungan kabohidrat menggunakan metode Spektrofotometri, protein menggunakan metode Kjeldahl, dan lemak menggunakan metode Soxhlet. Hasil penelitian menunjukkan bahwa kadungan gizi Tambelo memiliki presentase gizi rata-rata karbohidrat 1,49%, protein 0,46% dan, lemak 6,4%. Kandungan gizi Tambelo yang dibudidayakan masyarakat desa Simatalu memiliki presentase karbohidrat dan protein yang rendah. Hal ini dipengaruhi oleh faktor media (kayu) tempat Tambelo hidup.
Keanekaragaman Pohon Riparian di Beberapa Mata Air Desa Soba Kabupaten Kupang Emiliana Eka Bano; Yoseph M Laynurak; Chatarina Gradict Semiun; Yulita Iryani Mamulak
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 4 No 3 (2023): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v4i3.281

Abstract

Vegetasi pohon riparian merupakan salah satu komposisi penyususun ekosistem riparian yang memiliki peranan vital dalam menjaga keanekaragaman hayati. Pohon riparian dapat ditemukan di sekitar sumber mata air. Tujuan penelitian ini untuk  mengetahui keanekaragaman vegetasi pohon riparian di mata air desa Soba kecamatan Amarasi Barat kabupaten Kupang. Metode yang di gunakan adalah Point-Quarter, dengan penarikan transek menjadi empat kuadran pengamatan. Di setiap kuadran dicatat jenis dan jumlah pohon, kemudian dilakukan pengukuran terhadap tinggi, diameter dan stratifikasi pohon, dan indeks lingkungan (Indeks Naturalness dan Indeks Hemeroby). Data yang di peroleh di analisis untuk memperoleh Indeks Kekayaan Jenis Margalef, Indeks Nilai Penting, dan Indeks Keankeragaman Shanon-Wiener. Hasil penelitian ditemukan 16 jenis pohon riparian dari 11 famili meliputi Euphorbiaceae, Arecaceae, Fabaceae, Myrtaceae, Annonaceae, Meliaceae, Menispermaceae, Moraceae, Malvaceae, Verbenaceae, dan Anacardiaceae. Famili Arecaceae merupakan jenis pohon riparian yang paling sering dijumpai. INP tertinggi mata air Oe Kou  dan Oe Kiu ditemukan pada pohon pinang (Areca sp.), dan mata air Oras pada pohon Kananga (Cananga odorata). Keanekaragaman vegetasi pohon riparian pada ketiga mata air desa Soba tergolong buruk dan sangat buruk, sedangkan  kekayaan jenis tergolong rendah sampai sedang.
Identifikasi Jenis Pohon Riparian di Sumber Mata Air Desa Merbaun Kecamatan Amarasi Barat Chatarina Gradict Semiun; Yulita Iryani Mamulak
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 5 No 1 (2024): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v5i1.352

Abstract

Pohon riparian merupakan salah satu komponen penyusun ekosistem riparian (seperti mata air). Riparian terrmasuk sebagai daerah konservasi khusus yang perlu dipertahankan vegetasi aslinya karena jika vegetasi riparian semakin menyusut yang berdampak pada penurunan keanekaragaman hayati dan hilangnya fungsi vegetasi riparian tersebut Tujuan penelitian ini adalah untuk mengetahui jenis-jenis pohon ripaian yang terdapat di sumber mata air desa merbaun, kecamatan Amarasi barat, kabupaten Kupang, Nusa tenggara Timur. Penggumpulan data terdiri dari tingkat kealamian dan  keanekaragaman spesies. Pengumpulan data jenis tingkat kealamian terhadap pohon riparian  mengunakan indeks naturaliness dan indeks hemeroby). penarikan sampel pohon menggunakan metode kuadrat (Quadrat Sampling Technique) yang dilakukan secara acak. Di setiap plot, dilakukan identifikasi tumbuhan. Pengenalan nama tumbuhan menggunakan nama lokal, yang dirujuk ke nama ilmiah atau nama binomial. Analisis data terdiri dari status endemisme, indeks kekayaan jenis (Dmg), indeks keanekaragaman Shanon- Wiener (H’), dan indeks dominansi (D). Hasil penelitian ditemukan 15 jenis pohon yang terdiri dari 11 jenis endemik dan 4 jenis eksotik. Dmg dan H’ tergolong sedang, dengan tidak ada jenis pohon yang mendominasi.
Keanekaragaman Gastropoda di Pantai Muko Wujo Kecamatan Larantuka Kabupaten Flores Timur Margaretha Malik; Yoseph M. Laynurak; Chatarina Gradict Semiun
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.480

Abstract

Gastropods act as environmental bioindicators because they are sensitive to changes in the aquatic environment. The purpose of this study was to determine the diversity of gastropod species on Muko Wujo Beach. Determination of sampling points using the purposive sampling method. Samples were taken at two different stations based on human activities. The stages in collecting data on gastropod species include observing gastropods found in a 1x1 m plot, counting the number, and then recording it for identification purposes. In addition, environmental parameters, namely temperature and pH, were measured. Data on gastropod species and environmental parameters were then analysed qualitatively and quantitatively. Qualitative analysis was carried out by describing the morphological characteristics of gastropods, and identification was carried out using the help of scientific articles and internet websites. Quantitative analysis was carried out by calculating the diversity index, dominance index and similarity index of gastropod species. The results showed that 29 types of gastropods were found on Muko Beach, Larantuka District, East Flores Regency, which were divided into 14 families and 7 Orders. The diversity index, dominance index, and similarity index of gastropod species on Muko Wujo Beach were classified as moderate.
Kualitas Bakteriologi (Fecal Coliform) Mata Air Desa Toobaun Kabupaten Kupang Gracyana Putri Sarifan; Chatarina Gradict Semiun; Yulita Iryani Mamulak
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.530

Abstract

Springs are groundwater that comes out on its own to the surface of the earth. Water sources originating from springs are generally suitable for direct consumption because they undergo natural purification. However, the high demand for clean water causes springs to experience anthropogenic pressure, which can reduce the quality of spring water. The study aimed to determine the Bacteriological quality of spring water in Toobaun village. The method used in this study was the Most Probable Number (MPN), using a series of five tubes with a variety of 5-5-5. The test included three stages, namely the prediction test, the confirmation test, and the complete test. The data obtained were presented in the form of a table, then compared with the MPN table value and analyzed descriptively by comparing the MPN value with the water quality standard, namely PP No. 22 of 2021 (class I). The results of the study showed that the Fecal Coliform value found was 220 MPN/100ml and did not meet the quality standards of PP No. 22 of 2021 (Class I). In the complete test using EMBA media, three types of bacteria were found that had characteristics similar to Escherichia coli and Salmonella sp. Klebsiella sp. Based on the results of water testing at the Toobaun spring, it cannot be consumed directly without first being processed.
Penurunan Total Coliform Mata Air dengan Desain Biofilter Limbah Organik Viennye Wilhelmina Djawa; Gracyana Putri Sarifan; Maria Astin Rosari Tahu; Chatarina Gradict Semiun
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.555

Abstract

Unconserved springs tend to be polluted by various organic wastes. Accumulation of organic waste causes water to be contaminated with coliform bacteria, thus reducing water quality. Biofilter technology can be applied to reduce the number of coliform bacteria. This study aimed to determine the ability of BioFilter K4Ser-Col (moringa seeds, rice husks and kesambi charcoal) to reduce total coliform in spring water. The research location was at the Riumata Spring, Nekbaun village, West Amarasi sub-district, Kupang district. The work consisted of preparing the BioFilter K4Ser-Col media, taking water samples, and testing MPN coliform. MPN coliform testing was done twice before and after the water filtration on the BioFilter K4Ser-Col media. The study's results showed a decrease in the MPN value before and after filtering. The MPN coliform value before filtering was 1600 MPN/100 mL. After filtered water on the BioFilter K4Ser-Col media, it decreased to 23.23 MPN/100 mL. This value had met the national water quality standards for the class I category based on PP No. 22 of 2021. Thus, the use of BioFilter K4Ser-Col media could greatly reduce the number of coliform bacteria in water.
Co-Authors Alexander Tage Amaliana Sago Bano, Emiliana Eka Belo, Joana Da Conceicao Bernadus Esli Salembeheu Catur Retnaningdyah Djawa, Viennye Wilhelmina Emiliana Eka Bano Emilianus Pani Emilianus Pani Endang Arisoesilaningsih Gaudensius U. U. Boli Duhan Gaudensius U.U.Boli Duhan Gracyana Putri Sarifan Laynurak, Yoseph M Laynurak, Yoseph M. Leonardus Banilodu Leonardus Banilodu Leonardus Banilodu Malik, Margaretha Manewalu, Agnes Margaretha Malik Maria Astin Rosari Tahu Oktovianus Edvict Semiun Pani, Emilianus Pea, Maria Carina Salembeheu, Bernadus Esli Sarifan, Gracyana Putri Siki, Theodosia Monika Stefanus Stanis Stefanus Stanis Tahu, Maria Astin Rosari Taloim, Netri Thresia Trivict Semiun Vebronia Liunima Vebronia Liunima Viennye Wilhelmina Djawa Wihelmina, Maria Wisrance Yeni, Yustina Yoseph M Laynurak Yoseph M. Laynurak Yoseph M. Laynurak Yulita Iryani Mamulak Yulita Iryani Mamulak Yulita Iryani Mamulak Yulita Iryani Mamulak Yulita Iryani Mamulak Yulita Iryani Mamulak