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All Journal Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi Jurnal Mandala Pharmacon Indonesia Jurnal Mandala Pengabdian Masyarakat KOPEMAS

Fitriana Fitriana

Fakultas Farmasi Universitas Muslim Indonesia

Author-ID : 2485653

Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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POTENSI ANTIBAKTERI EKSTRAK ETANOL JAMUR KANCING (Agaricus bisporus) SEBAGAI ANTIBAKTERI Siska Nuryanti; Fitriana Fitriana
Jurnal Fitofarmaka Indonesia Vol 5, No 1 (2018): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

A research had been done on antibacterial activity of extract and the aim was to determine the antibacterial activity and inhibitory potency. The research was started by screening test using Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus epidermidis, Shigella dysenteriae, Staphylococcus aureus, Streptococcus mutans and Vibrio cholerae. Results showed that the Agaricus bisporus ekstract inhibited Bacillus subtilis, Shigella dysenteriae, Staphylococcus aureus, and Vibrio cholera at concentrations 0,1% and 1%. The results obtained Minimum Inhibitory Concentration (MIC) Agaricus bisporus extract against at concentrations of 0,1%. In the Minimum Bactericidal Concentration (MBC) test Agaricus bisporus extract against at concentrations of 0,8%. Agaricus bisporus extract has antibacterial activity wich was indicated by the inhibitory zone diameter. The biggest inhibitory zone diameter of Agaricus bisporus contained in bacteria Bacillus subtilis on the concentration of 6.4% (13 mm)

ISOLASI FUNGI ENDOFIT KULIT BUAH MERAH (Pandanus conoideus Lam.) YANG BERPOTENSI SEBAGAI ANTIBAKTERI SECARA METODE KLT - BIOAUTOGRAFI Reza Ariyadi Deponda; Fitriana Fitriana; Siska Nuryanti; Herwin Herwin
As-Syifaa Jurnal Farmasi Vol 11, No 2 (2019): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Red fruit (Pandanus conoideus Lam.) belongs to genus Pandanus. Red fruit oil has the activity as an antibacterial. The research aimed to obtain endophytic fungi isolates on the red fruit peel and assay the antibacterial activity by TLC-Bioautography. The research was experimentally conducted including the process of isolation of endophytic fungi, purification, macroscopic examination, screening for antibacterial activity against Staphylococcus aureus and Salmonella typhi, fermentation for 7, 14 and 21 days, separation and antibacterial assay by TLC-Bioautography against Staphylococcus aureus and Salmonella typhi, the isolate fermentate of endophytic fungi for 7 days showed an Rf value of 0.49 against Staphylococcus aureus and an 0.47 against Salmonella typhi. The isolate fermentate of endophytic fungi for 14 days showed an Rf value of 0.58 against Staphylococcus aureus and an 0.69 against Salmonella typhi. The isolate fermentate of endophytic fungi for 21 days showed an Rf value of 0.69 against Staphylococcus aureus and an 0.67 against Salmonella typhi. In conclusion, red fruit peel had endophytic fungi isolate with the potency as an antibacterial.

AKTIVITAS ANTIOKSIDAN EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT BIJI BUAH KENARI (Canarium indicum L.) SECARA KLT-AUTOGRAFI Nurul Khatimah Naim; Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 13, No 2 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i2.780

Walnut seeds (Canarium indicum L.) contain tannin, flavonoids, carotenoids which is potentially fungtioning as antioxidants. The research aimed to examine the antioxidant activity of fermentate extract of walnut seeds endophytic fungi by autography-TLC. The result of the endophytic fungi isolation in Walnut seeds (Canarium indicum L.) obtained 5 isolates with characteristics. The test result of screening free antiradical activity obtained that isolate 4th was active as an antioxidant indicated by the presence of brown spots with the addition of 1,1-diphenyl-2-picrylhydrazyl 0.004%. Isolate 4th was fermented using Maltosa Yeast Broth (MYB) medium to obtain fermentate supernatant and mycelia. The Fermentate of supernantant of isolate 4th was extracted to obtained supernantant of ethyl acetate extract. Based on the result of free antiradical activity test of ethyl acetate fermentate extract isolate 4th by autography-TLC using ethyl acetate: methanol: water eluent (6:2:1), obtained Rf 0.78 value active as free antiradical by spraying 1,1diphenyl-2-picrylhydrazil 0.004%.

ISOLASI BAKTERI PENGHASIL SELULOSA DARI BUAH-BUAHAN DIPASAR TRADISIONAL MAKASSAR Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. The objective of this research is to adding diversity of microorganism that can producing cellulose and observe it’s both optimum temperature and pH. First step is isolation of bacteria from Watermelon, Papaya, Cantaloupe, Mango, Dragon fruit, and Banana that found in traditional market in Makassar using Hestrin-Schramm (HS) agar. Each colony bacteria have been separated to making pure culture using HS agar then the colony was screening in HS broth to obtain colony bacteria that producing cellulose. Screening test found that 10 isolate that can produce cellulose which is MB-B02, MS-B01, MS-B03, NB-B03, PB-B01, PB-B02, PB-B03, PB-B04, PS-B01, and PS-B03. Then the colony that producing cellulose was optimize its temperature and pH. Cellulose obtained from optimization was measure it’s weight to determine optimum temperature and pH. Based from optimization found that 250C was optimum temperature for MS-B01, MS-B03, NB-B03, dan PB-B04, and 370 was optimum temperature for MB-B02, PB-B01, PB-B03, PS-B01, dan PS-B03, meanwhile for isolate PB-B02 optimum both in 250C dan 370C dan pH optimum for all isolate is pH 6

POTENSI LIMBAH AIR TAHU ASAL KOTA MAROS SEBAGAI SUMBER BAKTERI PENGHASIL ENZIM PROTEASE DALAM MELAWAN RADIKAL BEBAS Fitriana Fitriana; Amirullah Amirullah; Nurul Ilmy; Dyah Nur Pratiwi; Andi Adelah Resmilasari; Nabila Adelina HM
As-Syifaa Jurnal Farmasi Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

This research aims to determine the protease enzyme-producing bacteria in Limbah Air Tahu and IC50 fermentat protease enzyme-producing bacteria in Limbah Air Tahu potentially fight free radicals. The methods in the study, which is taking Limbah Air Tahu and sewage treatment, followed by isolation of the bacteria producing the enzyme protease. Bacterial isolates obtained purified, for macroscopic examination. Fermentation bacteria and the production of protease enzymes. Purified protease enzyme obtained. Testing fermentat to fight free radicals with the DPP method and the determination of IC50 values fermentat protease enzyme-producing bacteria. The results of the protease enzyme producing bacteria identification on Biochemical activity test, there were 7 positive bacteria. The results of screening anti-free radical activity against isolates obtained 12 isolates with code FF 05. UMI IBPLT most excellent activity that is obtained of the IC50 fermentat isolates UMI IBPLT FF 05 is equal to 0.129 and dibandingakan denagn IC50 Vitamin C is = 14.81 + 0:10 then isolates FF 05 UMI IBPLT active in fighting free radicals.

PENELUSURAN FUNGI ENDOFIT SEBAGAI PENGHASIL SENYAWA ANTIBIOTIKA DARI DAUN NANAS (Ananas comosus (L) Meer) Fitriana Fitriana; St. Maryam; Tadjuddin Naid; Maryana Maryana
As-Syifaa Jurnal Farmasi Vol 8, No 1 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

A search endophytic fungi from the leaves of pineapple (Ananas comosus (L) Merr) as a producer of antibiotic compounds will be carried out in order to obtain the endophytic fungi that have the ability to produce bioactive compounds useful as antibiotics.. Results of endophytic fungi isolates obtained six fungal isolates (IJA 1, IJA 2, IJA 3, IJA 4, IJA IJA 5 and 6), which have a different macroscopic characterization of forms, edge and elevation colonies. Isolates pure microbial fermentation results in the test antibiotic activity against some bacteria using agar diffusion test. All isolates pure fermentation results showed inhibitory activity against all bacteria in test as Bacillus subtilis, Esherichia coli, Pseudomonas aeruginosa, Salmonella thypi, Shigella dysenteriae, Staphylococcus aureus, Streptococcus mutans, and Vibrio cholerae. The inhibition of fungi isolates shown in (IJA 4) with a 26 mm zone of inhibition against Esherichia coli bacteria.

PROFIL BIOAUTOGRAM AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT JAMUR LAUT PADA ALGAE Kappaphycus alvarezii SECARA KLT BIOAUTOGRAFI Fitriana Fitriana; Rusli Rusli
As-Syifaa Jurnal Farmasi Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

The Research Chromatogram Profile Of Activities Antibacterial Extracts Isolates Marine Fungi in Algae Kappaphycus alvarezii by TLC Bioautography has been done with the intention of doing the screening test of pathogenic bacteria that can be inhibited by the extract of F3 (extract liquid culture and extract mycelia) and extracts of E (extract liquid culture and extract mycelia ) and see chromatogram profile of liquid culture extract of F3 on bacteria Escherichia coli and mycelia extract of E on bacteria Bacillus subtilis. This research was conducted purification of Fungi isolates E and F3 followed by fermentation process of fungi isolates for 3 weeks. Then proceed with the extraction process using an ethyl acetate solvent. The solvent was evaporated to obtain a dry extract, followed by a screening test of antibacterial activity against bacterial pathogenic, antibacterial activity test by TLC Bioautography to liquid culture extract of F3 and extract mycelia of E. The results of the screening test with a concentration of 250 µg was obtained to extract liquid culture of F3 and E provide activity against 5 pathogenic bacteria to extract mycelia while F3 and E provide activity against two bacterial pathogens. Separation of compounds by TLC using the ethyl acetate eluent: ethanol: water (8: 2: 1). The result of TLC Bioautography test of liquid culture extract of F3 to bacteria Escherichia coli and mycelia extract of E on bacteria Bacillus subtilis showed the existence of antibacterial activity.

UJI AKTIVITAS ANTIOKSIDAN ISOLAT FUNGI ENDOFIT DAUN GALING-GALING (Cayratia trifolia L.) DENGAN METODE 1,1-DIPHENYL-2-PICRYLHYDRAZIL (DPPH) Magfirah Abdullah; Fitriana Fitriana; St. Maryam
As-Syifaa Jurnal Farmasi Vol 12, No 2 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v12i2.617

Endophytic fungi generally produce secondary metabolites that have beneficial biological activities such as anticancer, antiviral, antibacterial or antioxidant compounds. The aim of this study was to examine the antioxidant activity of galling-galing leaf endophytic fungi isolate (Cayratia trifolia L.) by 1,1-diphenyl-2-picrylhydrazil (DPPH) method. This study used IDGG-03 endophytic fungi isolate fermented for 21 days using MYB medium then extracted and evaporation was carried out. The fermentate extract obtained was then tested qualitatively and quantitatively for antioxidant activity. Qualitative results using TLC plates indicate that galling-leaf fungi isolates have antioxidant activity which is characterized by the presence of yellow spots on a purple background. And in the quantitative test IC50 68,133 µg / mL was obtained, which indicated the antioxidant activity of galling-vane leaf endophytic fungi isolate (Cayratia trifolia L.) classified as a strong antioxidant.

KARAKTERISASI ISOLAT BAKTERI PENGHASIL SELULOSA DARI BUAH NAGA MERAH (Hylocereus polyrhizus) Siska Nuryanti; Fitriana Fitriana; A. Rini Pratiwi
As-Syifaa Jurnal Farmasi Vol 13, No 1 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i1.770

Cellulose is a natural polymer widely used for general industrial purposes. The development of science utilizes bacteria from certain fruits to produce biosellulose. Red dragon fruit (Hylocereus polyrhizus) contains a lot of sugar which can be bioconverted to cellulose by cellulose- producing lines. The research aimed to obtain bacteria from red dragon fruit with the potential to produce cellulose, then conduct morphological and biochemical characterization. The research was conducted by the isolation of bacteria from the fruit, purification, and screening tests using Hestrin-Schramm medium. The isolates obtained were characterized morphologically through macroscopic and microscopic observation and the biochemically through catalase test, carbohydrate fermentation, starch and gelatin hydrolysis, motility, and IMViC (Indole, Methyl red, Voges- Proskauer, Citrate). The results showed that 4 bacterial isolates had the potential to produce cellulose. Based on Bergey's Manual of Systematic Bacteriology, 3 bacterial genera were obtained, namely Acetobacter, Gluconacetobacter, and Azotobacter. The results of the characteristics of the isolates obtained at IBBN 2-2 were positive in the catalase test, sucrose and lactose fermentation, and methyl red. IBBN 3-1 was positive in the catalase test, sugar fermentation, starch hydrolysis, methyl red, and citrate. IBBN 3-2 was positive in the sucrose and glucose fermentation test, gelatin hydrolysis and 4-1 IBBN was positive in the sucrose fermentation test and gelatin hydrolysis.

AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI AKAR MANGROVE (Rhizophora apiculata Blume) SECARA KLT BIOAUTOGRAFI Fitriana Fitriana; Eka Nurshitya
As-Syifaa Jurnal Farmasi Vol 9, No 1 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Endophytic fungi have the ability to produce bioactive compounds one of which is antibacterial. A search of bioactive compounds in this research are the first was isolated endophytic fungi on the roots of mangrove then be purified and macroscopic order to obtain pure 6 isolates endophytic fungi. Proceed with the screening test to test bacteria and endophytic fungal isolates obtained first by IJM 2 code which provides activity against 5 test bacteria with the formation of the largest inhibition zone diameter. Then proceed with the fermentation of isolates IJM 2 for 21 days at room temperature using Potato Dextrose Broth medium after the separation to obtain a supernatant and mycelia. Then proceed with the solvent extraction using ethyl acetate and the extract obtained extract supernatant and mycelia. The extract obtained is then followed by TLC-Bioautografi testing using the eluent n-butanol: acetic acid: water (8: 1: 2). Results chromatogram profile with KLT-Bioutografi method showed antibacterial activity against bacteria Shigella dysentriae, Pseudomonas aeruginosa, Salmonella thypii, Vibrio cholerae, Stahpylococcus aureus.

Co-Authors A. Rini Pratiwi Adinda S. Gaffar Amirullah Amirullah Andi Adelah Resmilasari Annisa Almagfirah Achmar As Adi Abdullah Audia Triani Olii Ayu Wulandari Ayyub Harly Nurung Bayu Putra Bayu Putra Dyah Nur Pratiwi Eka Nurshitya Fadelita Huath Fhahri Mubarak Harlyanti Muthma'innah Mashar Hendra Herman Herwin Herwin Herwin Herwin Ismail Ismail Magfirah Abdullah Maryana Maryana Mirawati Mirawati Nabila Adelina HM Naura Amalia Saliha Nur Sukma Dwi Priyanti Nurul Hasanah Nurul Ilmy Nurul Khatimah Naim Nurung, Ayyub Harly Putri Lestari Rachmat Kosman Rasma Maret Halina Restu Islamia Rasyak Reza Ariyadi Deponda Rizqi Nur Azizah Rusli Rusli Rusli Rusli Safriani Rahman Siska Nuryanti Sitti Amirah St. Maryam Tadjuddin Naid Tadjuddin Naid

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