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UJI IN VITRO DAN IN SILICO SENYAWA 5,7,2’,5’-TETRAHYDROXY FLAVAN-3-OL TERHADAP ENZIM ALPHA GLUCOSIDASE Frengki Frengki; Deddi Prima; Fatma Sri Wahyuni; Daan Khambri; Henny Vanda; Noni Zakiah; Jamilah Abbas; Berna Elya
Jurnal Fitofarmaka Indonesia Vol 5, No 2 (2018): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Several of Calophyllums genus have been searched and proven as medicinal plants and Calophyllum macrophyllum is one of its genus. We have been isolated a compound from ethyl acetate fraction of the stem-bark. The compound was flavan-3-ol (5,7,2’,5’-tetrahydroxy flavan-3-ol). This research aimed to determine inhibition antidiabetic activity and affinity of its compound on α-glycosidase enzyme. In vitro antidiabetic effect shown by IC50 9.10 µg/ml and docking by Arguslab 4.01 shown by ΔG -10,24 kkal/mol as in silico test. The inhibition activity of flavan-3-ol against the α-glucosidase enzyme that is very strong shows its promising potential as a candidate for antidiabetic drugs.

The potential antioxidant activity of ethanolic extract of Aceh ant-plant (Mymercodia sp) on the free radical DPPH(1,1-Diphenyl-2-pikrylhidrazil) . Suryawati; . Frengki; Hijra Novia Suardi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.51 KB)

This research aims to investigate the potential antioxidant activity of ethanolic extract of Aceh ant-plant (Mymercodia sp) on the free radical DPPH (1,1-diphenyl-2-pikrylhidrazyl). The ant-plant was extracted using maceration technique with the ethanol as solvent. The extract was made in various concentrations: 5, 10, 25, and 50 ppm. Then DPPH solution was added to it. Absorbance reading was conducted with spectrophotometry at the wave length of 517 nm after 30 minutes. The result of research shows the potency of ant-plant (Mymercodia sp) as an antioxidant free radical scavenging ethanolic extract with IC50 value of 14.73 ppm. The ascorbic acid as the control showed IC50 value of 7:32 ppm.

Inhibitory of activity -glucosidase from ethyl acetate and flavanal compound of the stem-bark of Calophyllum mcrophyllum Scheff . Frengki; Jamilah Abbas; Surya Wati
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.509 KB)

Most of Calophyllum genus have been researched and proven as medicinal plants. Calophyllum macrophyllum Scheff is an Indonesia original plant growing on Sumatera and we found in Kerinci mount. One flavanal has been isolated from ethyl acetate fraction from the stem-bark of Calophyllum macrophyllumScheff. The compound is 5,7,2’,5’- tetrahedrons flavan-3-ol, brown crystal with melting point 300oC. The compound was extracted using maceration technique, then fractionated using chromatography and purified by crystallization using two solvents. Molecule structure was determined using physical data and spectroscopy methods including UV, IR and 1H and 13C-NMR. Ethyl acetate fraction and isolate were assayed for inhibitory activity on -glycosidase enzyme. The potential inhibition effect is got each other with IC50 26,198 =g/ml and 12,19 =g/ml.

In silico analysis of wild-type and mutant KRAS Frengki Frengki; Dedi Prima Putra; Fatma Sriwahyuni; Daan Khambri; Henni Vanda
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The mutations of the KRAS gene at codons 12, 13, and 61 have been widely reported with different prognosis. In silico is one approach to explain the characteristics of the mutant genes. This study aimed to reveal the potential energy and fluctuations of the binding site and active site of wild-type KRAS (KRAS Wt) and mutant KRAS (KRAS Mt) at codons 12, 13, and 61. The samples used in this study were the sequences of KRAS Wt and KRAS Mt genes, which were subjected to in-silico analysis that included molecular homology, docking, and dynamics using MOE, PyMOL, and online CABS servers. The results showed that fluctuations in the binding site of all KRAS Mt were lower than that of KRAS Wt. On the contrary, the active site (switch I and switch II) of KRAS Mt fluctuated more widely than KRAS Wt. The potential energy of KRAS Mt before forming a complex with GTP was higher (p<0.01) than KRAS Wt. After this formation, it remained higher at codons 12 and 61 but lower at codons 11 and 13 (p <0.001). Mt G12A did not show any changes. The higher fluctuations in the switch I and switch II regions and the post energy of KRAS-GTP complexes may explain why types of cancers with mutations at codons 11 and 13 have a better prognosis than those with mutations at codons 12 and 61.

Prediction of diacerein inhibition activity against interleukin-1 receptors through docking method and tracing of pharmacokinetic profiles and their toxicity Frengki Frengki; Vivi Sofia; Deddi Prima Putra; Fatma Sri Wahyuni; Daan Khambri; Henni Vanda
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

IL-1 is one of the cytokines involved in joint diseases such as osteoarthritis. IL-1 plays a role in maintaining the balance of proteolytic proteins:MMPs and TIMPs inhibitors. Increased expression and uncontrolled IL-1 activity tend to increase the role of MMPs in degrading proteoglycans and joint tissue collagen. This study aims to reveal the interaction model of one of the osteoarthritis drugs, namely diacerein. A drug belongs to a group of disease-modifying osteoarthritis drugs (DMOADs) to suppressthe development of the disease rate, improving the structure and function of the cartilage and surrounding tissue. "In silico" digital test uses the technique of "molecular docking: used as a method of the approach using the MOE 2007.09 software application. The test material was in the form of a diacerein 3D structure and five control ligands, while the IL-1Î² / IL-1RI receptor template was downloaded from pdb.org (PDB ID: 1ITB). The ligand pharmacokinetic profile will also be displayed obtained through the ADMETSAR server. The docking results showed diacerein had the lowest docking score of -12.3285 kcal/mol with the strongest affinity, the best pharmacokinetic profile but more toxic. This study proves that the mechanism of diacerein inhibition of IL-1Î² / IL-1RI receptors is similar to dexamethasone, prednisolone,and minocycline.

Aktifitas Hepatoprotektif Ekstrak Etanol Daun Sirsak (Annona Muricata L.) Terhadap Kerusakan Hati Tikus yang Diinduksi dengan Parasetamol Noni Zakiah; Yanuarman Yanuarman; Frengki Frengki; Munazar Munazar
AcTion: Aceh Nutrition Journal Vol 2, No 1 (2017): AcTion Vol 2 No 1 Tahun 2017
Publisher : Jurusan Gizi Politeknik Kesehatan Kemenkes Aceh

For a long period, the therapy of liver diseases that carried out against liver diseases is still a challenge for modern medicine. The drugs used in the treatment of liver diseases such as antiviruses, diuretics, and antibiotics could cause some serious side effects. Annona muricata plants or better known as soursop plant has long been widely used as traditional medicine. This study was aimed to determine the hepatoprotective effects of ethanol extract of soursop (Annona muricata L.) leaves on liver histologist pattern of the mice of Sprague-Dawley thread that exposed to high doses of paracetamol. The dosages of the ethanol extract of soursop leaves of 200 mg/Kg BW, 400 mg/Kg BW and 600 mg/Kg BW orally for 14 days, then 2 hours after induction of final dose, induced with paracetamol 2.5 g/kg BW orally. Furthermore, through the observation of mice liver histologic, the level of damages in mice liver cells, including conditions of hepatocytes, necrosis, pyknocytosis, karyorrhexis, karyolysis were recorded. The results showed that ethanol extract of soursop leaves with a dose of 600 mg/kg BW showed significant hepatoprotective effects characterized by a relatively normal hepatocytes condition after exposure to high doses of paracetamol. The final conclusion was that the ethanol extract of soursop leaves provide hepatoprotective effects.Keywords: Annonamuricata L., hepatoprotective, paracetamol, liver histologySelama ini terapi yang dilakukan terhadap penyakit hati masih merupakan tantangan bagi pengobatan modern. Obat-obat yang biasa digunakan pada pengobatan penyakit hati diantaranya adalah antivirus, diuretic dan antibiotik yang dapat menimbulkan beberapa efek samping yang serius. Tumbuhan Annona muricata (sirsak) telah lama digunakan sebagai obat tradisional yang luas. Penelitian ini bertujuan untuk mengetahui efek hepatoprotektif ekstrak etanol daun sirsak (Annona muricata L.) terhadap gambaran histologis hati tikus putih galur Sprague dawley yang terpapar parasetamol dosis tinggi. Dosis yang digunakan adalah ekstrak etanol daun sirsak (EEDS) dosis 200 mg/kg BB, 400 mg/kg BB dan 600 mg/kg BB per oral selama 14 hari, kemudian 2 jam setelah pemberian dosis akhir, diinduksi dengan parasetamol 2,5 g/kg BB per oral. Selanjutnya dilakukan pengamatan histologi hati tikus, dicatat tingkat kerusakan pada sel hati tikus, meliputi kondisi hepatosit, nekrosis, piknositosis, karioreksis, kariolisis. Hasil penelitian menunjukkan bahwa ekstrak etanol daun sirsak dosis 600 mg/kg BB memperlihatkan efek hepatoprotektif yang signifikan yang ditandai dengan kondisi hepatosit yang relatif normal setelah terpapar dengan dosis tinggi parasetamol. Dari hasil penelitian disimpulkan pemberian ektrak etanol daun sirsak memberikan efek hepatoprotektifKata kunci: Annona muricata L., hepatoprotektif, parasetamol, histologi hati

POTENTIAL ANTIVIRAL OF CATECHINS AND THEIR DERIVATIVES TO INHIBIT SARS-COV-2 RECEPTORS OF M pro PROTEIN AND SPIKE GLYCOPROTEIN IN COVID-19 THROUGH THE IN SILICO APPROACH Frengki Frengki; Deddi Prima Putra; Fatma Sri Wahyuni; Daan Khambri; Henni Vanda; Vivi Sofia
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Catechin and their derivatives have been studied to have antiviral potential against COVID-19 through in silico method “pharmacokinetics screening and molecular docking”. Pharmacokinetics and toxicity profiles were obtained through the ADMETSAR server and SwissADME server. Then proceed with the prediction of affinity through the method molecular docking using the software application MOE 2007.09. The testmaterial is in the form of a 3D catechin structure and its derivatives as well as several control ligands downloaded via Pubmed. While templatethe Receptor M pro protein and the Spike glycoprotein are downloaded from pdb.org (PDB ID: 6LU7 and 6LXT). The pharmacokinetic profile of catechins is relatively better than all control ligands with the lowest toxicity. Molecular docking results also show that catechins and theirderivatives have a stronger affinity than control ligands. This research proves that catechin has antiviral potential through inhibition of Mpro protein and Spike glycoprotein COVID-19 virus.

POTENTIAL ANTIVIRAL OF CATECHINS AND THEIR DERIVATIVES TO INHIBIT SARS-COV-2 RECEPTORS OF M pro PROTEIN AND SPIKE GLYCOPROTEIN IN COVID-19 THROUGH THE IN SILICO APPROACH Frengki Frengki; Deddi Prima Putra; Fatma Sri Wahyuni; Daan Khambri; Henni Vanda; Vivi Sofia
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.16652

Catechin and their derivatives have been studied to have antiviral potential against COVID-19 through in silico method “pharmacokinetics screening and molecular docking”. Pharmacokinetics and toxicity profiles were obtained through the ADMETSAR server and SwissADME server. Then proceed with the prediction of affinity through the method molecular docking using the software application MOE 2007.09. The testmaterial is in the form of a 3D catechin structure and its derivatives as well as several control ligands downloaded via Pubmed. While templatethe Receptor M pro protein and the Spike glycoprotein are downloaded from pdb.org (PDB ID: 6LU7 and 6LXT). The pharmacokinetic profile of catechins is relatively better than all control ligands with the lowest toxicity. Molecular docking results also show that catechins and theirderivatives have a stronger affinity than control ligands. This research proves that catechin has antiviral potential through inhibition of Mpro protein and Spike glycoprotein COVID-19 virus.

The Effect of Ethanol Extracted of Balsamina (Impatiens balsamina Linn) leaf on the Growth of Candida albicans In Vitro Nadia Naitullah; Faisal Jamin; Frengki Frengki; Maryulia Dewi
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i2.3351

This research aimed to determine the effect of ethanol extract of Impatiens balsamina Linn leaves on the growth of Candida albicans in vitro. One kilogram of Impatiens balsamina leaves which grows in Aceh was used. The leaves were washed, dried and blended into powder. The powder was then extracted with ethanol, filtrated, and evaporated. The crude extract was then made into serial concentration of 100, 75, 50, and 25%. The test of the effect of giving ethanol extract in Impatiens balsamina Linn is determined by the Kirby-Bauer disc diffusion method. Results showed that inhibition zones for 0, 25, 50, 75, and 100% were 0.0, 8.66±1.53, 11.66±1.55, 13.66±1.52, and 6±5.29 consecutively. It can be concluded that the ethanol extract of Impatiens balsamina Linn leaves± inhibit the growth of Candida albicans.Key words: Candida albicans, balsamina leaf, Impatiens balsamina Linn, extract

Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach Frengki F; Roslizawaty R; Desi Pertiwi
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3338

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening

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