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Andi Dahliaty
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Materials Science & Nanotechnology Mathematics Physics

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Journal : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

 1 
Produksi Enzim Lakase Oleh Jamur Trichoderma Asperellum Lbkurcci Dengan Variasi Penambahan CuSO4 Menggunakan Bioreaktor Tray Secara Solid State Fermentation (SSF) Nurliati, Ivanna; Helianty, Sri; Dahliaty, Andi
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production and lignin degradation in pulp. There are diverse sources of laccase producing like fungi, plants and bacteria. This research is, conducted to the production of laccase enzyme using Trichoderma asperellum LBKURCC1 with bioreactor tray using solid state fermentation (SSF) method with rice straw substrate. The research was purposed to determine the effect of CuSO4 inducer and know the best fermentation time on production of enzyme by Trichoderma asperellum LBKURCC1. Fermentation was carried out with time variations that are 5-10 days and variations of CuSO4 that are 0,25-0,75 g/L with substrate size ±0,5 cm, the bed depth on the tray 3 cm. Fermentation using room temperature and pH is 5,5. The result showed that the highest laccase enzyme activity was obtained on CuSO4 inducer 0,50 g/L and 7 days fermentation with average laccase enzyme activity was 19,270 U/L.Keywords: Inducer, Laccase, Rice straw, Solid state fermentation, Trichoderma asperellum.
Produksi Enzim Lakase Oleh Jamur Trichoderma Asperelloides LBKURCC2 Menggunakan Substrat Jerami Padi Secara Fermentasi Kultur Padat Dengan Variasi Waktu Fermentasi Dan Laju Aerasi 1,5 L/M Iwara, Bangkit Swadi; Helianty, Sri; Dahliaty, Andi
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 8 (2021): Edisi 1 Januari s/d Juni 2021
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) is classified as blue copper oxidase enzyme. Laccase generally found in plant, insect, bacteria and filamentous fungus. One of filamentous fungus that able to degradates lignin is Trichoderma. Lignin is available in biomass such as rice straw, that contains around 18% lignin. The technique of producing laccase enzymes in this study is solid state fermentation. Solid state fermentation allows microorganisms to grow in conditions close to or similar to their natural habitat, with relatively better product than submerged state fermentation. In this study, the effect of giving force aeration on variations in fermentation time was studied in order to obtain optimum conditions for the production of laccase enzyme by solid state fermentation using the fungus Trichoderma asperelloides LBKURCC2 with rice straw substrate on a tray bioreactor. The fermentation is carried out with variation of the fermentation time 6, 8, and 10 days with incubation temperature of 30 ± 2 ºC, acetate buffer solution pH 5.5 (0.05 M), substrate size 1 cm, addition of 0.5 g / l CuSO4.7H2O inducer, bed height 3 cm, and aeration rate 1.5 l / m. The highest laccase enzyme activity was obtained during the production time of 8 days, with an average 7.78 x 10-6 U / L. The 8th day is considered to be the best growth time where the enzyme activity has reached its peak and is in a stagnant condition after the increase that occurred on the 6th and 7th days. Meanwhile, on the 9th and 10th days there was a significant decrease in the activity of the laccase enzyme due to the greater repression of catabolites.Keywords: Laccase, Solid State Fermentation, Tray Bioreactor, Force Aeration, FermentationTime
Produksi Enzim Lakase Oleh jamur Trichoderma Asperellum Lbkurcci Dalam Bioreactor Tray Menggunakan Variasi Ukuran Substrat Jerami Padi Dan Induser CuSO4 Pada Fermentasi Kultur Padat Gustina Gustina; Sri Helianty; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production and lignin degradation in pulp. There are diverse sources of laccase producing like fungi, plants and bacteria. In this research, the production of lacase enzyme using Trichoderma asperellum LBKURCC1 with bioreactor tray using solid state fermentation (SSF) method with rice straw substrate. The purpose of this study was to determine the effect of rice straw size and the addition of CuSO4 concentration to the highest production of lacase enzyme by Trichoderma asperellum LBKURCC1. Fermentation is carried out with variation of time 5, 6, 7, 8, 9 and 10 days with fermentation temperature ± 30ºC, substrate size ± 0.5 cm, 1.5 cm, and 3.0 cm with substrate thickness at tray ie 3 cm acetate buffer solution pH 5,5 and addition of CuSO4 0.5 g/l. Variations for the addition of CuSO4 concentration 0 g/l, 0.50 g/l and 1 g/l with substrate thickness on tray were 3 cm, size of rice straw 0.5 cm and acetate buffer solution pH 5.5. Small size can provide the highest value and the added concentration of CuSO4 given can increase the activity of the resulting lacase enzyme. However, too high CuSO4 concentrations result in decreased lacase enzyme activity. The results showed that the highest lacase enzyme activity was obtained on the size of rice straw ie 0.5 cm and 7 days of fermentation time with an average of 19.27 U/L and the highest lacase enzyme activity was obtained on CuSO4 0.50 g/l and fermentation time 7 day with an average of 19.27 U/L.Keywords: Inducer, Laccase, Rice straw, Solid state fermentation, Trichoderma asperellum.
Produksi Enzim lakase Oleh Jamur Tricoderma Asperellum Lbkurcci Secara Fermentasi Padat Pada Bioreactor Tray Muhamad Fajri; Sri Helianty; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Laccase enzyme is classified as oxidoreductase enzyme; i.e. an enzyme that can oxidize phenolic compounds and reduce oxygen molecules into water molecules. An enzyme production process using fungi through solid fermentation has more advantages compared to the one through submerged fermentation since it has the processing condition as if the fungi grow in nature. This research is aimed at investigating the process of laccase enzyme production by Trichoderma asperellum LBKURCC1 in bioreactor tray through solid state fermentation using rice straw substrates. The objective of this research is to figure out the influence of bed depth and fermenting time on laccase enzyme production by Trichoderma asperellum LBKURCC1. The variables of the research are the bed depth of 1.5, to 4.5 cm and fermentation time of 5, to 10 days. The research findings show that the peak of laccase enzyme activity was obtained at 3.0 cm bed depth and 7 day fermentation time. Increasing the depth of the bed can increase the activity of laccase enzyme produced. However, increasing too much depth of the bed can cause the decrease of laccase enzyme activity. Besides, the fermentation time over 7 days can also decrease the laccase enzyme activity for the amount of the water contained in the bed is drying up.Keywords: bioreactor tray, laccase enzyme, rice straw, Trichoderma asperellum LBKURCC1
Co-Authors Adolf Hiskia Nainggolan Amilia Linggawati Anita R Sidabutar Christine Jose Dwi Cahyaningsih Febry Kurniawan Feliatra Halida Sophia Haryati Haryati Ilham ' Itnawita ' Iwara, Bangkit Swadi Maria Erna Kustyawati Masdalena Sinaga Maya Dahlena Muhamad Fajri Nasution, Nurul Iflah Novianty, Riryn Nur Azlina Nurliati, Ivanna Nurul Khumaida Popy Wardani Puspita Sari Rizki Wulandari Silvera Devi Siregar, Siti Saidah Sri Helianty Susilawati, Susilawati Titania T. Nugroho Titania Tjandrawati Nugroho Waidil Anuar Yuana Nurulita Yum Eryanti