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All Journal Indonesian Journal of Cancer Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara
Lia Damayanti
Departement of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Stimulation Effect of Exosome From Healthy Sera to Natural Killer (NK) Cells of Hepatocellular Carcinoma Subject In Vitro Deby, Deby; Antarianto, Radiana Dhewayani; Barasila, Atikah Chalida; Irawan, Cosphiadi; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Damayanti, Lia; Ritchie, Ni Ken; Aditya, Robby Nur
Indonesian Journal of Cancer Vol 18, No 4 (2024): December
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i4.1122

Abstract

Background: Hepatocellular carcinoma has a poor prognosis due to limitations of therapy such as late diagnosis, lack of specific biomarkers, and insensitivity to this tumor agent. This study aims to develop immunotherapy using autologous natural killer cells (NK cells) with exosome stimulation for hepatocellular carcinoma patients, addressing treatment limitations.Methods: Experimental research conducted from October 2022 to June 2023 at Universitas Indonesia’s Faculty of Medicine involved three hepatocellular carcinoma patients at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. NK cells from hepatocellular carcinoma patients were isolated from peripheral venous blood, and exosomes were isolated from the blood serum of healthy donors. Exosome characterization with a particle size analyzer and flow cytometry. Stimulation of exosomes on NK cells for 24 hours, then evaluation of expression of NKp44, NKp46, NKp30, NKG2D, KIR2D, and NKG2A receptors, as well as perforin and granzyme B expression. Visualization of interactions of NK cells with other mononuclear cell fractions (CD4, CD8, CD11c, and CD19) by immunofluorescence. The study compares stimulated and unstimulated NK cells, analyzing their expression of activated and inhibitory receptors, using either the One-Way Anova parametric test or the Kruskal-Wallis non-parametric test for non-normally distributed data.Results: Particle size 100 nm, negative electric charge, and CD63+CD81+ (double positive) exosome isolated results. There was increased expression of receptors NKp44, NKp46, NKp30, NKG2D, decreased expression of NKG2A, and increased expression of perforin and granzyme B in exosome-induced NK cells. There was no cell interaction in the form of immune synapses between exosome-induced NK cells and other mononuclear cell fractions in hepatocellular carcinoma patients. Conclusions: Induction of exosomes into NK cells of hepatocellular carcinoma patients restores the cytotoxic ability of NK cells
PERAN RASIO LACTATE DEHIDROGINASE DAN LIMFOSIT SEBAGAI PENANDA EFUSI PLEURA MALIGNANSI: THE ROLE OF LACTATE DEHYDROGENASE AND LYMPHOCYTE RATIO AS MARKERS OF MALIGNANT PLEURAL EFFUSION Pasaribu, Rouly Pola; Damayanti, Lia
Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara Vol. 24 No. 2 (2025): Juli 2025
Publisher : Faculty of Medicine Universitas Islam Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/ibnusina.v24i2.913

Abstract

Pendahuluan: Efusi pleura merupakan salah satugejala dari patologi yang mendasarinya. Pemeriksaan biokimia yang umumnya dilakukan untuk membedakan patologi tersebut adalah kadar laktat dehydrogenase, adenosine deaminase, dan limfosit. Tujuan: mengevaluasi penggunaan rasio LDH cairan pleura/limfosit dalam cairan pleura sebagai parameter baru untuk membedakan antara efusi pleura maligna dan bukan. Metode: Penelitian cross sectional di RSUP Dr. Mohammad Hoesin (RSMH) Palembang menggunakan rekam medis. Pasien dewasa dengan diagnosa efusi pleura eksudatif yang dilakukan pemeriksaan analisa cairan pleura libatkan dalam penelitian ini. Uji hipotesis dilakukan dengan menggunakan uji T tidak berpasangan atau uji Mann Whitney U. Hasil: Penelitian ini melibatkan 206 pasien, dimana 43,21% didiagnosis dengan efusi pleura non-malignansi, sedangkan 56,79% dengan efusi pleura malignansi. Pemeriksaan LDH cairan pleuradan limfosit cairan pleura menunjukkan perbedaan yang signifikan antara kelompok pasien dengan dan tanpa malignansi (LDH cairan pleurakelompok malignansi: 593,5 U/L [32 – 1.940] vs kelompok non-malignansi: 198 U/L [104 – 811], p < 0,001; limfosit kelompok non-malignansi: 87% [21-95] vs kelompok malignansi: 38% [30-93], p < 0,001). Rasio LDH cairan pleura terhadap limfosit juga menunjukkan perbedaan yang signifikan (kelompok malignansi: 15,18 [0,81-60,03] vs non-malignansi: 2,84 [1,15-22,87], p < 0,001). Kesimpulan: Terdapat perbedaan yang signifikan antara LDH cairan pleura, limfosit cairan pleura, dan rasio antara kedua antara pasien dengan efusi pleura maligna dan non-maligna.
Co-Authors Aditya, Robby Nur Ahani, Ardhi Rahman Antarianto, Radiana Dhewayani Arleni Arleni Arleni Bustami Barasila, Atikah Chalida Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Deby Deby Des Suryani Isabella K. Liem Isnani A. Suryono Jeanne A. Pawitan Jinia Lilianty Ni Ken Ritchie, Ni Ken Ni M. Swantari Pasaribu, Rouly Pola Radiana D. Antarianto Reza Y. Purwoko Sigit Purbadi Twidy Tarcisia, Twidy Yefta Moenadjat