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All Journal Damianus Journal of Medicine Indonesian Journal of Cancer Makara Journal of Health Research Makara Journal of Science Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Barasila, Atikah Chalida
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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CD34+ UCB stem cells attenuate TGF-β signaling and inhibit liver fibrosis: A new avenue for liver cirrhosis-carcinogenesis prevention Septiana, Wahyunia Likhayati; Antarianto, Radiana Dhewayani; Louisa, Melva; Jusuf, Ahmad Aulia; Barasila, Atikah Chalida; Pawitan, Jeanne Adiwinata; Fasha, Iqbal
Makara Journal of Health Research Vol. 24, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: The liver microenvironment plays a key role in liver fibrosis and carcinogenesis. This study aimed to fill the gap in knowledge on the interaction between hepatic stellate cells and endothelial progenitor cells with biomarkers of liver fibrosis and/or carcinogenesis, including Col1A1, TGF-β, and tenascin-C. Methods: CD34+ stem cells were isolated from umbilical-cord-blood mononuclear cells. 2D and 3D co-culture of CD34+ UCB SCs and LX2 was performed. The cells were incubated in a CO2 incubator for three days. Morphological observation, qRT-PCR of TGF-β1 and COL1A1, and immunocytochemistry of tenascin-C were performed. Results: CD34+ UCB SCs were viable in the 2D and 3D co-culture for 24 h. 3D co-culture of CD34+ UCB SCs and LX2 inhibited in vitro liver fibrosis by lowering Col 1A1 expression as compared to control. We observed lower TGF-β expression in 3D co-culture on days 1 and 2 followed by higher expression of TGF-β on day 3. 2D co-culture of CD34+ UCB SCs and LX2 showed a different level of COL1A1 and TGF- β expression compared with 3D co-culture. Spheroids from 2D co-culture of CD34+ UCB SCs and LX-2 showed immunoreactivity against tenascin-C. Conclusion: Interaction between LX-2 and CD34+ UCB SCs in 3D co-culture inhibits in vitro liver fibrosis. The viability of CD34+ UCB SCs is essential for attenuation of TGF-β signaling in LX-2.
Association between Macrophage’s Cell Number and Maternal Factors in Human Milk Khudri, Ghaniyyatul; Sukmawati, Dewi; Barasila, Atikah Chalida; Suryandari, Dwi Anita
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

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Infant nutrition and immune protection require the consumption of human milk, with macrophages mainly contributing to immune defense and infant development. This study was performed to investigate the correlation between macrophage cell numbers in human milk and various maternal factors. Thirteen human milk samples were collected from lactating mothers and were evaluated for macrophage cell numbers using a hemacytometer. The recorded questionnaire containing several questions, including age, multivitamin consumption, coronavirus disease 2019 vaccination status, allergy history, and lactation duration, was used to determine the maternal factors. Pearson’s correlation and Mann-Whitney test was employed in statistical analysis, with p-values < 0.05 considered significant. The macrophage cell number reached 54.236 ± 7.456 cells per mL. Notably, a significant correlation was observed between maternal allergy history and the number of macrophage cells in human milk (p = 0.049). Meanwhile, no statistically significant associations were detected among multivitamin consumption (p = 0.833), vaccination status (p = 0.923), and lactation duration (p = 0.236). This study emphasizes the effect of maternal characteristics on the composition and immune properties of human milk. The specific mechanisms underlying these correlations and their potential influence on infant health and development must be explored in future research.
Stimulation Effect of Exosome From Healthy Sera to Natural Killer (NK) Cells of Hepatocellular Carcinoma Subject In Vitro Deby, Deby; Antarianto, Radiana Dhewayani; Barasila, Atikah Chalida; Irawan, Cosphiadi; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Damayanti, Lia; Ritchie, Ni Ken; Aditya, Robby Nur
Indonesian Journal of Cancer Vol 18, No 4 (2024): December
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i4.1122

Abstract

Background: Hepatocellular carcinoma has a poor prognosis due to limitations of therapy such as late diagnosis, lack of specific biomarkers, and insensitivity to this tumor agent. This study aims to develop immunotherapy using autologous natural killer cells (NK cells) with exosome stimulation for hepatocellular carcinoma patients, addressing treatment limitations.Methods: Experimental research conducted from October 2022 to June 2023 at Universitas Indonesia’s Faculty of Medicine involved three hepatocellular carcinoma patients at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. NK cells from hepatocellular carcinoma patients were isolated from peripheral venous blood, and exosomes were isolated from the blood serum of healthy donors. Exosome characterization with a particle size analyzer and flow cytometry. Stimulation of exosomes on NK cells for 24 hours, then evaluation of expression of NKp44, NKp46, NKp30, NKG2D, KIR2D, and NKG2A receptors, as well as perforin and granzyme B expression. Visualization of interactions of NK cells with other mononuclear cell fractions (CD4, CD8, CD11c, and CD19) by immunofluorescence. The study compares stimulated and unstimulated NK cells, analyzing their expression of activated and inhibitory receptors, using either the One-Way Anova parametric test or the Kruskal-Wallis non-parametric test for non-normally distributed data.Results: Particle size 100 nm, negative electric charge, and CD63+CD81+ (double positive) exosome isolated results. There was increased expression of receptors NKp44, NKp46, NKp30, NKG2D, decreased expression of NKG2A, and increased expression of perforin and granzyme B in exosome-induced NK cells. There was no cell interaction in the form of immune synapses between exosome-induced NK cells and other mononuclear cell fractions in hepatocellular carcinoma patients. Conclusions: Induction of exosomes into NK cells of hepatocellular carcinoma patients restores the cytotoxic ability of NK cells
The Role of Acupuncture and Stem Cell Therapy in Enhancing Stroke Recovery Dimara, Imelda; Barasila, Atikah Chalida
Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education Research Institute Vol. 8 No. - (2024): Proceedings Book of International Conference and Exhibition on The Indonesian M
Publisher : Writing Center IMERI FMUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69951/proceedingsbookoficeonimeri.v8i-.245

Abstract

Stroke is an acute neurological disorder caused by disrupted blood flow to the brain, depriving cells of oxygen. From 1990 to 2019, it was the world’s second-leading cause of death and the third-leading cause of combined death and disability. Acupuncture plays a role in stem cell treatment by activating the body's natural recovery processes through physical stimulation, aiding the recovery and rehabilitation of stroke patients. Several studies have explored combining stem cell therapy with acupuncture, yielding promising results in improving patient outcomes. This review assesses the effectiveness of combining acupuncture with stem cell therapy for stroke recovery. Analysis of seven randomized controlled trials shows that both stem cell transplantation and acupuncture contribute to the rehabilitation of stroke patients. When applied together, this combined therapy improves the survival, retention, and functional differentiation of implanted stem cells, creating a synergistic effect that amplifies the benefits of each treatment and compensates for their individual limitations. Key acupuncture points for this combination therapy include GV20, GV14, LI11, ST36, and GV26, with treatment durations ranging from 10 to 22 days.
Structure, morphology, and role of primo vascular system Deby, Deby; Barasila, Atikah Chalida
Bahasa Indonesia Vol 24 No 1 (2025): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v24i1.4984

Abstract

Introduction: Kim Bong Han in 1960 introduced the primo vascular system (PVS), a novel circulatory system independent of the circulatory and lymphatic systems. This system is believed to have a relationship with classical acupuncture points and meridians which underlie the mechanism of acupuncture in healing diseases. This study aims to better understand the structure, morphology and role of the primo vascular system.    Methods: This study obtained 30,336 articles from international journals, including Google Scholar, Proquest, Scopus, and Pubmed, focusing on the Primo Vascular System. 12 journals were analyzed, including original English articles published between 2019-2022, open access, and free full text. Results: This study discovered that primo vascular system has a distinct structure and shape in the form of milky-white, semi-transparent spots with primo vessels and primo nodes inside of which primo fluid circulates and contains a variety of cells, including primo microcells that contain DNA. Primo Vascular System can play a role in tissue regeneration, innate immunity in inflammatory processes, and extramedullary hematopoiesis. Conclusion: The primo vascular system, consisting of milky-white, semi-transparent spots with primo vessels and nodes, plays a crucial role in tissue regeneration, innate immunity, and extramedullary hematopoiesis.
Co-Authors Aditya, Robby Nur Ahani, Ardhi Rahman Ahmad Aulia Antarianto, Radiana Dhewayani Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Deby Deby Dewi Sukmawati Dimara, Imelda Dwi Anita Suryandari Fasha, Iqbal Ghaniyyatul Khudri Lia Damayanti Melva Louisa Ni Ken Ritchie, Ni Ken Pawitan, Jeanne Adiwinata Wahyunia Likhayati Septiana