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All Journal Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Journal of Epidemiology and Public Health Medical Technology and Public Health Journal Medicra (Journal of Medical Laboratory Science/Technology) Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Jurnal Abdimas Kartika Wijayakusuma Procedia of Social Sciences and Humanities Prosiding University Research Colloquium Academia Open
Puspitasari Puspitasari
Universitas Muhammadiyah Sidoarjo
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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POTENTIAL MOLECULES AGAINST COVID-19 FROM ANNONA MURICATA; AN IN-SILICO APPROACH Miftahul Mushlih; Andika Aliviameita; Puspitasari Puspitasari; Ahmad Shobrun Jamil
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 6 No 1 (2022): Medical Technology and Public Health Journal March 2022
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v6i1.3069

Abstract

At the end of 2019, a new virus emerged from Wuhan, China named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The key to viral infection to host cells is the use of the ACE2 receptor. This study aimed to explore the potential of drugs in Annona muricata plant using the Insilico method. Compounds obtained from KNApSAcK Based on the analysis using SwissADME (Lipinski creteria)criteria. 22 compounds passed the selection. Eight ligands were identified as being able to change the conformation of the initial form of RBD and ACE2 attachment. Eight molecules are able to change the initial conformation, namely Asimilobine, Coreximine, (+)-Stepharine, Coclaurine, Norcorydine, N- Nornuciferinhe, methoxyphenyl)methyl]-1,2,3,4-tetrahydroisoquinolin-5-ol, and Atherosperminine.
Pengenalan Pemeriksaan Berbasis Nucleic Acid Amplification Tests (NAATs) Di Sekolah Kesehatan Miftahul Mushlih; Puspitasari Puspitasari; Andika Aliviameyta
Darmabakti : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 4 No 2 (2023): Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Lembaga Peneliian dan Pengabdian Masyarakat (LPPM) Universitas Islam Madura (UIM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/darmabakti.2023.4.2.153-159

Abstract

Perkembangan pemeriksaan terbaru banyak mengarah dengan memanfaatkan kode unik dan spesifik yang ada di DNA. Pemeriksaan ini dikenal luas dengan nukleic acid amplification tests (NAATs). Kesenjangan pendidikan antara perkembangan teknologi dengan penerapan di sekolah memunculkan suatu masalah tersendiri. Tujuan dari kegiatan ini untuk melakukan pengenalan dan workshop secara langsung metode NAATs oleh Prodi teknologi laboratorium medis (TLM), UMSIDA kepada SMK Mitra Sehat Sidoarjo. Kegiatan dilakukan melalui tiga tahap, yaitu penjajakan untuk mengetahui tingkat implementasi pengenalan NAATs di sekolah, Pengenalan dasar teori pemeriksaan, dan Workshop secara langsung, yaitu siswa diajak untuk melakukan praktikum secara langsung pada kasus identifikasi Toxoplasma gondii. Selain dengan melatih keahlian siswa, kemampuan siswa juga diukur melalui pretest dan posttest. Berdasarkan analisis yang dilakukan terdapat peningkatan pengetahuan dan kemampuan siswa dalam mengenal dan melakukan pemeriksaan NAATs.
Variations In Methods For Storing Samples From Diabetes Mellitus Patients On The Results Of Urine Leukocyte Examination Maulidiyah , Hikmatul; Puspitasari
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 8 No 2 (2024): Medical Technology and Public Health Journal September 2024
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v8i2.4872

Abstract

Diabetes mellitus (DM) is often accompanied by both microvascular and macrovascular. One of the common complications  in patients with diabetes mellitus is a urinary tract infection. The purpose of this study was to determine the difference between using a 10% formaldehyde preservative and storage at 4°C to delay urine leukocyte examination in diabetic patients. This study employed  an  analytical observationalmethod using urine samples from patients with diabetes mellitus. A total  of six samples were obtained from Siti Fatimah Tulangan 'Aisyiyah Hospital. The research was conducted from May to June 2023. The results  indicate that there are differences in the results of urine leukocyte examinations in diabetes mellitus patients based on the variations in sample storage methods.   Keywords: Delayed, diabetes mellitus, leukocytes, storage, urine.
Acute Toxicity Test Against Mice (Mus Musculus) On The Lempuyang Gajah Extract (Zingiber Zerumbet (L.) Roscoe Ex Sm.) With Sgot Sgpt Parameters: Uji Toksisitas Akut Terhadap Mencit (Mus Musculus) Pada Ekstrak Lempuyang Gajah (Zingiber Zerumbet (L.) Roscoe Ex Sm.) Dengan Parameter SGOT SGPT Sofi Rahayu, Bella; Rohmah, Jamilatur; Rini, Chylen Setiyo; Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 7 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v7i1.1656

Abstract

Lempuyang Gajah (Zingiber zerumbet (L.) Roscoe ex Sm) is a medicinal plant that has many benefits in the medicinal industry. Useful as a tonic, external medicine, anti-seizure and appetite enhancer. This study aims to determine the toxicity of the lempuyang gajah. This research uses laboratory experimental methods. The lempuyang gajah plant was obtained in the village of Terungkulon, Krian. The initial stage was the administration of lempuyang gajah extract with various doses of 4000, 5000 and 6000 mg/kg BB orally, consisting of 5 treatment groups. Parameters observed, changes in toxic symptoms, macroscopic examination and parameters of SGOT, SGPT. The results were analyzed using the One Way ANOVA test. The results of the study after giving the extract did not cause death so it was included in the non-toxic category, there were several symptoms such as weakness and seizures. The levels of SGOT, SGPT showed normal average results, statistically there was no effect (p > 0.05). Macroscopic results showed no changes or abnormalities in the liver. So that the ethanol extract of lempuyang gajah can be said to be safe for humans.
Insilico study of Phyllanthus niruri potency as an antiviral Sars Cov 2: Omicron and delta variants : Analisis Potensi Phyllanthus niruri sebagai antivirus Sars Cov 2: varian Omicron dan delta secara insilico Mushlih , Miftahul; Aliviameita, Andika; Puspitasari; Firmansyah, Nukayo; Murosidah, Pratasya Liyaajul
Procedia of Social Sciences and Humanities Vol. 3 (2022): Proceedings of the 1st SENARA 2022
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pssh.v3i.251

Abstract

Corona Virus Disease 19 (Covid 19) caused by infection with severe acute respiratory syndrome coronavirus 2 (Sars Cov-2). In its development, this virus has several variants due to mutations, including the Omicron (B.1.1.529) and delta (B.1.617.2) variants of concern, one of the keys to infection with this virus is the angiotensin receptor enzyme 2 (ACE-2). This study aimed to explore the potential of Phyllanthus niruri plant insilico. The active plant components were explored from the KNackSAcK family, then selected using Lipinski criteria using SWISS ADME, docking using HEX 8.0. The results of the analysis showed that 5 of the 21 compounds met Lipinski's criteria, i.e Hypophyllanthin, Hinokinin, 4-Methoxynorsecurinine, Nirtetralin, & Lintetralin. Nirtetralin & 4-Methoxynorsecurinine were able to change the binding conformation of RBD-ACE2 in the Omicron variant, while Hinokinin was able to change the binding conformation of RBD-ACE2 in the delta variant. These results were different from the first discovered sars cov 2, in which Hinokinin, 4-Methoxynorsecurinine, & Nirtetralin were able to change the conformation of RBD-ACE2.
The Effectiveness of Acupressure Combination of Temulawak in Increased Toddler Appetite: Efektifitas Akupresur Kombinasi Temulawak Dalam Peningkatan Nafsu Makan Balita Faridah Hanum, Sri Mukhodim; Widowati, Hesty; Puspitasari; Filberta, Yusnita; Tjondro, Lidia Agustin
Procedia of Social Sciences and Humanities Vol. 3 (2022): Proceedings of the 1st SENARA 2022
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pssh.v3i.340

Abstract

Prolonged difficulty eating in children results in a decrease in the calorie intake needed so that it can affect the growth and development of children. Based on data from the Weighing Month in August 2020 in Java, it was found that the percentage of toddlers wasting was 8.0%. Acupressure and family medicinal plants, namely ginger, can be non-pharmacological therapy options to grow children's appetite. Acupressure is a form of energy stimulation by providing massage and stimulation at certain points on the body, while temulawak can increase appetite in patients with primary anorexia because temulawak contains curcumin which is able to correct abnormalities in bile, so that the process of absorption of food in the intestine is easier. good. The purpose of this study was to determine the effectiveness of acupressure combination of ginger in increasing the appetite of toddlers aged 1-5 years. The design used is a pre experimental design with a quantitative approach with a Wilcoxon pre and post test method. The variables in this study were acupressure and temulawak with appetite. The population in this study were children aged 1 to 5 years with a sample of 20 children Acupressure was performed 6 times (2 times a week) while ginger was given 1 time/day at a dose according to age and body weight for 3 weeks. The assessment was carried out twice before and after treatment with questionnaires and weight scales. Furthermore, the analysis of the two data was carried out with the Wilcoxon pre and post test. The results of this study indicate that there is a significant difference after treatment with a value of P = 0.000 (P<0.05), which means that there is an effect of acupressure and ginger on increasing toddlers' appetite based on weight gain.
Penguatan Peran Guru Teknik Laboratorium Medik Bidang Hematologi: Routine Hematology Laboratory Test untuk mendukung SDG’s Puspitasari, Puspitasari; Aliviameita, Andika; Ardiansyah, Syahrul; Puspita, Anggun; Ramadhan, Syahrul Ibnu Mubaroq
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 1 (2025): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i1.564

Abstract

Hematologi merupakan bidang kedokteran yang paling dinamis, dan dapat berubah-ubah tergantung pada perkembangan ilmu pengetahuan dan teknologi terkini. Diagnosis penyakit juga didukung oleh pemeriksaan hematologi. Guru sebagai pendidik memiliki tanggung jawab besar dalam memastikan bahwa siswa memperoleh pemahaman yang komprehensif dan keterampilan praktis yang memadai. Pengabdian masyarakat ini bertujuan untuk meningkatkan kompetensi guru dan siswa SMK Muhammadiyah 1 Pandaan Jurusan Teknik Laboratorium Medik (TLM) yang berfokus pada penguatan peran guru dalam memberikan pembelajaran praktikum bidang hematologi khususnya terkait Routine Hematology Laboratory Test untuk mendukung Sustainable Development Goals (SDG’s). Pengabdian Masyarakat dilaksanakan pada Juli - Agustus 2024 yang diikuti oleh 2 guru dan 53 siswa. Hasil dari kegiatan ini yaitu terdapat peningkatan kompetensi baik guru maupun siswa pada pembelajaran laboratorium bidang hematologi yang dapat dievaluasi melalui pretest dan post-test. Dengan target yang terukur, program pengabdian masyarakat ini memberikan kontribusi nyata terhadap pengembangan ilmu pengetahuan dan praktik pendidikan di bidang kesehatan, serta mendukung pencapaian Sustainable Development Goals (SDG’s) melalui perbaikan kualitas pendidikan dan kesehatan.
Relationship between Blood Glucose, Cholesterol and Blood Pressure in Diabetes Mellitus Patients with Diabetic Ulcers: Hubungan Glukosa Darah, Kolesterol Dengan Tekanan Darah Pada Pasien Diabetes Melitus Dengan Ulkus Diabetikum Siti Khosnaini; Puspitasari
Academia Open Vol. 4 (2021): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.4.2021.2074

Abstract

Diabetes mellitus is a chronic disease caused by heredity or environment. According to Riskesdas, 2018 states that based on a doctor's diagnosis, the number of diabetes mellitus cases in Indonesia has increased to 2% compared to the results of Rikesdas in 2013 which was only 1.5%. The purpose of this study was to determine the relationship between blood glucose, cholesterol and blood pressure in diabetes mellitus patients with diabetic ulcers. The research design used was cross sectional. The research sample was 30 patients with diabetes mellitus with diabetic ulcers at Wonoayu Luka House. This research was conducted from February to March 2021. The results showed that there was no relationship between blood glucose and cholesterol with blood pressure (r=0.043, r=-0.199) after the Pearson correlation test was performed.
Enhancing Medical Lab Education via Molecular Biology: A Service Learning Approach for Toxoplasmosis Detection: Meningkatkan Pendidikan Laboratorium Medis melalui Biologi Molekuler: Pendekatan Pembelajaran Berbasis Pelayanan untuk Deteksi Toksoplasmosis Miftahul Mushlih; Puspitasari; Sitti Nur Qablyatin Syabandia; Wa Ode Asma`Ul Husna Huesein; Andika Aliviameyta
Academia Open Vol. 8 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.8.2023.7229

Abstract

Penelitian ini bertujuan untuk meningkatkan pemahaman siswa SMK Teknologi Lab Kedokteran dalam ranah pengembangan pemeriksaan kesehatan melalui integrasi teknik biologi molekuler. Menggunakan kerangka Service Learning (SL), mahasiswa dibimbing dalam memahami evolusi metode diagnostik yang berkaitan dengan bidangnya, selama periode Februari hingga Maret 2023. Kuliah dan diskusi memperkenalkan mahasiswa pada aplikasi biologi molekuler dalam berbagai ujian, sementara lokakarya terarah pendekatan memungkinkan aplikasi langsung dari metode ini untuk pengujian toksoplasmosis. Prosesnya meliputi ekstraksi DNA dari sampel wajah kucing liar menggunakan Chelex 100 Grade Resin, menargetkan gen B1 untuk PCR, menggunakan siklus PCR minimal, dan memvisualisasikan hasil PCR melalui elektroforesis gel agarosa 1%. Hasil, menunjukkan identifikasi toksoplasma positif dalam 5 dari 6 sampel, menggarisbawahi kemanjuran pendekatan ini. Analisis pra dan pasca tes menunjukkan peningkatan pengetahuan yang signifikan di antara peserta, menekankan implikasi metodologi pemeriksaan medis lanjutan dalam pendidikan dan praktik. Highlight: Integrasi Biologi Molekuler: Menekankan penggabungan teknik biologi molekuler ke dalam pendidikan laboratorium medis untuk meningkatkan kemampuan diagnostik dan pembelajaran siswa. Pendekatan Service Learning: Menyoroti penggunaan Service Learning sebagai metode yang efektif untuk menjembatani pengetahuan teoretis dengan aplikasi praktis, menumbuhkan pemahaman yang lebih dalam tentang pengembangan pemeriksaan medis. Identifikasi Toksoplasmosis: Menampilkan keberhasilan penerapan metode molekuler, seperti ekstraksi PCR dan DNA, untuk mengidentifikasi keberadaan toksoplasma dalam sampel kucing liar, menunjukkan potensi deteksi penyakit yang akurat. Kata kunci: Pendidikan Lab Medis, Biologi Molekuler, KKN, Deteksi Toksoplasmosis, Peningkatan Pengetahuan
Co-Authors Ahmad Shobrun Jamil Aliviameyta , Andika Ambar Mudigdo Andika Aliviameita Andika Aliviameyta Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Chylen Setiyo Rini Dian Delta Dina Trikumalasari Dira Nadila Evi Rinata Faridah Hanum, Sri Mukhodim Filberta, Yusnita Firmansyah, Nukayo Huesein, Wa Ode Asma`Ul Husna Jamilatur Rohmah Livia Nur Azzah Maulidiyah , Hikmatul Meryn Linanda Agustin Miftahul Mushlih Murosidah, Pratasya Liyaajul Mushlih , Miftahul Nevy Elensya Puspita, Anggun Qilmia Fanani Ramadhan, Syahrul Ibnu Mubaroq Rita Adriani Benya Adriani Rohman Dijaya Silvia Ariyanti Siska Aprilia Siti Khosnaini Siti Nofiani Mufida Siti Zaenab Salim Sitti Nur Qablyatin Syabandia Sofi Rahayu, Bella Sri Mukhodim Faridah Hanum Syabandia, Sitti Nur Qablyatin Tjondro, Lidia Agustin Wa Ode Asma`Ul Husna Huesein Widowati, Hesty Yanik Purwanti