Garuda - Garba Rujukan Digital

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line Soilia Fertilita; Willy Sandhika; Desak Gede Agung Suprabawati
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

Comparison of Recurrence Rates in Patients with Locally Advanced Breast Cancer after Mastectomy and Received Aromatase Inhibitor Therapy with Steroids and Nonsteroids I Ketut Yante; Desak Gede Agung Suprabawati; Iskandar Ali
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 10 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i10.360

Introduction. Breast cancer is a problem that is still faced throughout the world. The incidence of cancer in Indonesia is at number 8 in Southeast Asia. The incidence of breast cancer in women is 42.1 per 100,000 population. Aromatase inhibitors are hormonal therapy used in postmenopausal breast cancer with positive ER and/or positive PR. The third generation aromatase inhibitors are divided into 2 categories, namely non-steroidal agents that are reversible and steroid agents that are irreversible. Based on existing studies, there are no consistent research results regarding the incidence of recurrence after aromatase inhibitor therapy, both steroids and non-steroidal. Methods. This study is an analytical observational study with a retrospective cross-sectional design using secondary data from medical records. The research was carried out at the Surgical Clinic of RSUD dr. Soetomo Surabaya on October 1, 2020 until the end with the subject of LABC patients at POSA Surgery RSUD Dr. Soetomo who has undergone mastectomy and radiotherapy and has received adjuvant hormonal aromatase inhibitor therapy for 2 years from January 2018 to January 2020. Results. In the nonsteroidal aromatase inhibitor group, 18 subjects (60%) experienced recurrence and 16 subjects in the steroid aromatase inhibitor group (32%) with an OR of 0.314 (0.12-0.81; p=0.014. Based on the results of multivariate analysis, it was found that the increased risk of recurrence was significantly affected only by the administration of aromatase inhibitor p= 0.052 Conclusion. Women with locally advanced breast cancer after mastectomy who received aromatase inhibitor steroid therapy had a 0.314 times lower risk of recurrence than those who received non-steroidal aromatase inhibitor therapy.

Correlation between Changes of NT-Pro BNP and HS-Troponin I Level with Cardiotoxicity in Locally Advanced Breast Cancer After Three Cycles of Neoadjuvant CAF Chemotherapy Cicilia Indriaty; Leonita Anniwati; J.Nugroho Eko Putranto; Desak Gede Agung Suprabawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1352

Chemotherapy with cyclophosphamide, adriamycin, and fluorouracil (CAF) regiment in patients with locally advanced breast cancer have a risk of cardiotoxicity. Cardiotoxicity examination standards using left ventricular ejection fraction (LVEF) by echocardiography are considered insensitive for detection of subclinical ventricular dysfunction. NT-pro BNP and Hs-Troponin I (hs-TnI) as cardiac biomarkers are expected to help detect early cardiotoxicity. This study intended to analyze the correlation between changes of NT-pro BNP and hs-TnI levels with cardiotoxicity in breast cancer after three cycles of chemotherapy.This was a cross-sectional observational study, conducted at the Dr. Soetomo General Hospital Surabaya. The subjects consisted of 23 breast cancer patients who underwent chemotherapy with CAF regiment. NT-proBNP and hs-TnI examination used CLIA methods (Immulite 1000, ADVIA Centaur TnI-Ultra). Cardiotoxicity based on decreased LVEF to more than 10% of the initial LVEF value using echocardiography. Significant increases in NT pro BNP and hs-TnI levels were obtained before and after treatment (p=0.000, p=0.002). A significant decrease in LVEF was obtained before and after treatment (p=0.000), but only 2 patients (8.7%) showed cardiotoxicity. There was no correlation between changes in NT-pro BNP and hs-TnI levels with changes in LVEF before and after chemotherapy (p=0.666 and r=0.095; p=0.254 and r=-0.28). There was no correlation between changes in NT-pro BNP and hs-TnI levels with cardiotoxicity, which was assessed based on LVEF reduction, in locally advanced breast cancer after three-cycles of chemotherapy with CAF regiment.

Comparison of Recurrence Rates in Patients with Locally Advanced Breast Cancer after Mastectomy and Received Aromatase Inhibitor Therapy with Steroids and Nonsteroids I Ketut Yante; Desak Gede Agung Suprabawati; Iskandar Ali
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 10 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i10.360

Introduction. Breast cancer is a problem that is still faced throughout the world. The incidence of cancer in Indonesia is at number 8 in Southeast Asia. The incidence of breast cancer in women is 42.1 per 100,000 population. Aromatase inhibitors are hormonal therapy used in postmenopausal breast cancer with positive ER and/or positive PR. The third generation aromatase inhibitors are divided into 2 categories, namely non-steroidal agents that are reversible and steroid agents that are irreversible. Based on existing studies, there are no consistent research results regarding the incidence of recurrence after aromatase inhibitor therapy, both steroids and non-steroidal. Methods. This study is an analytical observational study with a retrospective cross-sectional design using secondary data from medical records. The research was carried out at the Surgical Clinic of RSUD dr. Soetomo Surabaya on October 1, 2020 until the end with the subject of LABC patients at POSA Surgery RSUD Dr. Soetomo who has undergone mastectomy and radiotherapy and has received adjuvant hormonal aromatase inhibitor therapy for 2 years from January 2018 to January 2020. Results. In the nonsteroidal aromatase inhibitor group, 18 subjects (60%) experienced recurrence and 16 subjects in the steroid aromatase inhibitor group (32%) with an OR of 0.314 (0.12-0.81; p=0.014. Based on the results of multivariate analysis, it was found that the increased risk of recurrence was significantly affected only by the administration of aromatase inhibitor p= 0.052 Conclusion. Women with locally advanced breast cancer after mastectomy who received aromatase inhibitor steroid therapy had a 0.314 times lower risk of recurrence than those who received non-steroidal aromatase inhibitor therapy.

Hubungan intensitas nyeri dengan kualitas hidup pasien kanker payudara yang menjalani kemoterapi di PPLK RSUD Dr. Soetomo Duta Angela; Djohan Ardiansyah; Desak Gede Agung Suprabawati
Intisari Sains Medis Vol. 13 No. 1 (2022): (Available Online : 1 April 2022)
Publisher : DiscoverSys Inc.

Introduction: Breast cancer occurs because breast cells grow out of control. The incidence of breast cancer is still very high both in developed countries and in Indonesia. One treatment that can be done is chemotherapy. Pain is one of the effects that can occur in undergoing chemotherapy, besides the increasing stage of cancer can increase pain. This increased pain often affects a person's quality of life. This study aims to determine the relationship between pain intensity and quality of life of breast cancer patients undergoing chemotherapy at PPLK RSUD Dr. Soetomo.Methods: This research is a descriptive analytical study with a cross-sectional method. Sampling was done by total sampling that met the inclusion and exclusion criteria, then filled out the Sf-36 questionnaire and the Wong Baker pain scale. Forty respondents with breast cancer underwent chemotherapy at PPLK RSUD Dr. Soetomo. Analysis using SPSS. The Shapiro Wilk test was used to determine the normality test. Correlation using Spearman's test.Results: From 40 respondents, the youngest age in this study was 29 years old and the oldest was 61 years old. The highest level of pain in stage 4 breast cancer patients is in the mildly disturbing category, which is measured using the Wong Baker scale. The results of the Spearman test concluded that there were significant results between pain intensity and quality of life. There are 8 components in assessing the quality of life with the SF-36: physical function, physical role, emotional role, energy, mental health, social function, pain, and general health (p<0.05).Conclusion: This study shows a relationship between pain intensity and quality of life of breast cancer patients undergoing chemotherapy. Pendahuluan: Kanker payudara terjadi karena sel-sel payudara tumbuh di luar kendali. Angka kejadian kanker payudara masih sangat tinggi baik di negara maju maupun Indonesia. Salah satu pengobatan yang dapat dilakukan adalah kemoterapi. Nyeri merupakan salah satu efek yang dapat terjadi dalam menjalani kemoterapi, selain itu stadium kanker yang meningkat dapat meningkatkan rasa nyeri. Nyeri yang meningkat ini seringkali memengaruhi kualitas hidup seseorang. Penelitian ini bertujuan untuk mengetahui hubungan intensitas nyeri terhadap kualitas hidup pasien kanker payudara yang menjalani kemoterapi di PPLK RSUD Dr. Soetomo.Metode: Penelitian ini merupakan penelitian deskripsi analitik dengan metode cross-sectional. Pengambilan sampel dilakukan dengan cara total sampling yang memenuhi kriteria inklusi dan eksklusi, kemudian dilakukan pengisian kuisioner Sf-36 dan skala nyeri Wong Baker. Terdapat 40 responden penderita kanker payudara yang menjalani kemoterapi di PPLK RSUD Dr. Soetomo. Analisis menggunakan SPSS. Uji Shapiro Wilk digunakan untuk menentukan uji normalitas. Korelasi hubungan menggunakan uji Spearman.Hasil: Dari 40 responden diperoleh hasil dengan usia termuda dalam penelitian ini berumur 29 tahun dan tertua berumur 61 tahun. Tingkatan nyeri tertinggi pada pasien kanker payudara stadium 4 masuk dalam kategori agak mengganggu, yang diukur menggunakan skala Wong Baker. Hasil uji Spearman menyimpulkan adanya hasil yang signifikan antara intensitas nyeri dengan kualitas hidup. Terdapat 8 komponen dalam menilai kualitas hidup dengan SF-36, yaitu fungsi fisik, peranan fisik, peranan emosi, energi, kesehatan jiwa, fungsi sosial, rasa nyeri, dan kesehatan umum (p<0,05).Kesimpulan: Penelitian ini menunjukkan adanya hubungan antara intensitas nyeri dan kualitas hidup pasien kanker payudara yang sedang menjalani kemoterapi.

Correlation between Changes of NT-Pro BNP and HS-Troponin I Level with Cardiotoxicity in Locally Advanced Breast Cancer After Three Cycles of Neoadjuvant CAF Chemotherapy Cicilia Indriaty; Leonita Anniwati; J.Nugroho Eko Putranto; Desak Gede Agung Suprabawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1352

Chemotherapy with cyclophosphamide, adriamycin, and fluorouracil (CAF) regiment in patients with locally advanced breast cancer have a risk of cardiotoxicity. Cardiotoxicity examination standards using left ventricular ejection fraction (LVEF) by echocardiography are considered insensitive for detection of subclinical ventricular dysfunction. NT-pro BNP and Hs-Troponin I (hs-TnI) as cardiac biomarkers are expected to help detect early cardiotoxicity. This study intended to analyze the correlation between changes of NT-pro BNP and hs-TnI levels with cardiotoxicity in breast cancer after three cycles of chemotherapy.This was a cross-sectional observational study, conducted at the Dr. Soetomo General Hospital Surabaya. The subjects consisted of 23 breast cancer patients who underwent chemotherapy with CAF regiment. NT-proBNP and hs-TnI examination used CLIA methods (Immulite 1000, ADVIA Centaur TnI-Ultra). Cardiotoxicity based on decreased LVEF to more than 10% of the initial LVEF value using echocardiography. Significant increases in NT pro BNP and hs-TnI levels were obtained before and after treatment (p=0.000, p=0.002). A significant decrease in LVEF was obtained before and after treatment (p=0.000), but only 2 patients (8.7%) showed cardiotoxicity. There was no correlation between changes in NT-pro BNP and hs-TnI levels with changes in LVEF before and after chemotherapy (p=0.666 and r=0.095; p=0.254 and r=-0.28). There was no correlation between changes in NT-pro BNP and hs-TnI levels with cardiotoxicity, which was assessed based on LVEF reduction, in locally advanced breast cancer after three-cycles of chemotherapy with CAF regiment.

GLUCOSE LEVELS OF PLEURAL EFFUSION FLUID AND HER2 STATUS IN PLEURAL-METASTATIC BREAST CANCER Muhammad Dhanny Irawan; Desak Gede Agung Suprabawati; Heru Purwanto
Majalah Biomorfologi Vol. 33 No. 2 (2023): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v33i2.2023.75-81

Highlights Patients with breast cancer who have distant metastases are frequently impacted by pleural effusion. HER2 status was found to be substantially correlated with glucose levels in pleural effusion fluid. Abstract Background: Patients diagnosed with breast cancer who have also been affected by distant metastases often suffer from pleural effusion. The prognosis of malignant pleural effusions worsens when glucose levels in the pleural fluid are low. Elevated levels of anaerobic glycolysis due to HER2 overexpression cause breast cancer cells to take in more glucose. Objective: This study aimed to identify any correlation between glucose levels in pleural fluid and HER2 status in breast cancer patients. Material and Method: In this research, 35 female patients with thoracocentesis pleural metastatic breast cancer participated in an analytical observational study using a cross-sectional design. Patients who had undergone thoracocentesis had their HER2 status determined based on the information included in their medical records in the Integrated Oncology Clinic (Poli Onkologi Satu Atap (POSA)) Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. After obtaining data on glucose levels in pleural fluid and HER2 status, statistical analysis was carried out to determine the relationship between the two parameters. Results: The statistical test results with a 95% significance level obtained p=0.004 (p<0.05), so it could be statistically said that there was a significant relationship between the glucose level of pleural fluid effusion with HER2 status. The obtained OR values were 10.93 and CI=95% (1.87-63.97), so that it could be interpreted that the low-glucose levels of pleural effusion fluid increase the proportion of patients with positive HER2 compared to not low-glucose levels of pleural effusion fluid. The correlation between the glucose level of pleural effusion fluid and HER2 status was moderately positive (Chi-Square Contingency Coefficient = 0.44). Conclusion: Glucose levels in pleural effusion fluid were significantly correlated with HER2 status.

Pengaruh Ekstrak Daun Sirsak terhadap Ekspresi CD8 pada Populasi Limfosit Tumor Payudara Tikus Sparaque Dawley Fertilita, Soilia; Sandhika, Willy; Suprabawati, Desak Gede Agung
Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Vol 6, No 2 (2020): JMK Yayasan RS.Dr.Soetomo, Kedua 2020
Publisher : STIKES Yayasan RS.Dr.Soetomo

Perubahan klinis pada payudara sebagian besar bersifat jinak, hanya 3 – 6% yang merupakan keganasan payudara. Namun demikian, kanker payudara menjadi perhatian utama mengingat angka kejadian yang terus menerus meningkat serta morbiditas dan mortalitas yang tinggi. Pengobatan kanker hingga saat ini masih menjadi tantangan. Hal ini membuka peluang penelitian terapi kanker terutama dari bahan herbal. Daun sirsak telah banyak digunakan masyarakat secara tradisional untuk pengobatan tumor. Di samping itu berbagai studi juga telah menunjukkan efek sitotoksik tanaman ini terhadap berbagi cell line kanker. Namun demikian belum ada penelitian yang menganalisis efek ekstrak daun sirsak terhadap populasi limfosit di lingkungan mikro tumor terutama yang berfungsi pada respon imun anti tumor. Penelitian ini bertujuan untuk mengetahui infiltrasi sel limfosit T CD8 pada jaringan tumor yang payudara tikus Sparaque Dawley yang diberikan ekstrak etanol daun sirsak. Ekspresi CD8 dianalisis melalui pewarnaan imunohistokimia. Ekspresi CD8 positif ditandai dengan warna coklat pada sitoplasma sel limfosit, selanjutnya ekspresi CD8 dari setiap sampel dihitung pada 3 lapang pandang dengan high-power field, kemudian ditentukan nilai rata-ratanya. Pewarnaan HE menunjukkan variasi infiltrasi limfosit pada nodul payudara tikus SD. Rata-rata ekspresi CD8 kelompok kontrol menunjukkan hasil lebih tinggi dibandingkan kelompok perlakuan. Namun demikian, hasil uji komparasi menunjukkan tidak ada perbedaan bermakna (p > 0,05) antara ekspresi CD8 kelompok kontrol dengan perlakuan. Hasil ini menunjukkan bahwa pemberian ekstrak daun sirsak tidak menyebabkan perbedaan bermakna ekspresi CD8 pada populasi limfosit yang menginfiltrasi tumor payudara tikus SD.

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search