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Simple Synthesis of Cellulose Triacetate from HVS Paper Waste and Its Application for Optode Arif, Zulhan; Fendy; Akhmad Aghzath, Ananjaya; Saprudin, Deden; Rohaeti, Eti
Indonesian Journal of Chemical Studies Vol. 3 No. 1 (2024): Indones. J. Chem. Stud., June 2024
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v3i1.46

Abstract

The optode membrane is a membrane that can identify ions in an aqueous solution. One of the most widely used optode membranes is cellulose triacetate based. Cellulose triacetate (CTA) has the characteristics of hydrophobic, transparent, elastic, and affordable. There are sources of cellulose triacetate that can be utilized and waste paper is one of them. Waste paper is extracted to obtain the cellulose, then by acetylization reaction to produce cellulose triacetate. The resultant cellulose triacetate has a degree of substitution (DS) of 2.89 and an acetyl group percentage (% AG) of 43.64. The standard and synthesized CTA optode membrane are tested for performance with various parameters, ie optimum pH, optimum immersion time, working range, limit of detection (LOD), and limit of quantification (LOQ). The standard CTA optode membranes and synthesized worked at pH 3 and 4 with optimum immersion time for 15 min, respectively. The optode produced a linear response in detecting Cr(VI) ion in the concentration range of 0.02-1 mg/L for standard CTA with an R2 of 0.9726 and 1-25 mg/L for synthesized CTA with an R2 of 0.9764, The limit of detection (LOD) and limit of quantitation (LOQ) were 0.0015 mg/L and 0.0051 mg/L for standard CTA, while 0.0224 mg/L and 0.0749 mg/L for synthesis CTA respectively. Since both optode membranes' performance test results are adequate, the synthesis results of CTA optode membranes can be employed as one source of cellulose triacetate.
Pembuatan Visual Strip Sensor Ion Besi(II) dengan Bahan Pendukung Kertas yang Dilapisi Poliakrilamida Kemala, Tetty; Saprudin, Deden; Permana, Ifri Surya
Jurnal Kartika Kimia Vol 7 No 1 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i1.259

Abstract

Penelitian ini bertujuan membuat visual strip sensor untuk mengukur kadar besi. Sensor ini dibuat dari kertas selulosa asetat yang telah dilapisi oleh poliakrilamida dan disisipi pengompleks 1,10-fenantrolin. Pengujian visual strip sensor dilakukan pada berbagai konsentrasi besi(II) dengan membuat deret standar dan melihat kisaran konsentrasi besi(II) yang dapat terbedakan oleh mata. Sensor yang telah diuji kemudian diamati kestabilan warnanya selama 4 hari. Visual strip sensor mampu membedakan dengan kisaran 5 ppm dan sedikit terjadi peningkatan intensitas warna setelah diamati selama 4 hari. Pengukuran sampel obat penambah darah dengan 3 kali ulangan menghasilkan keterulangan yang baik. Dari hasil pengukuran visual strip sensor dibandingkan dengan spektrometri serapan atom diperoleh ketepatan 99,42%.
EFFECT OF MAGNETITE AND CHITOSAN-MAGNETITE ADDITION ON BACTERIAL LEVELS AND NH3-N (FREE-LIVING N2-FIXING BACTERIA CULTURE) Umar, Ali; Saprudin, Deden; Hazra, Fahrizal
Jurnal Kimia Riset Vol. 8 No. 2 (2023): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v8i2.47647

Abstract

Free-living N2-fixing bacteria are essential in the soil because they provide a source of nitrogen in the form of ammonium needed by plants to be used in building protein blocks. The fixation of free N2 needs to be increased by adding nanomaterials such as magnetite (Fe3O4), which has an active group and can be a source of nitrogenase enzyme cofactor. In this study, increased N2 fixation used N2-fixing bacteria in Burks medium. This increase was determined by measuring NH3 levels using the Phenate method formed after Burks medium was treated with Fe3O4 and chitosan-magnetite (CS-Fe3O4). The study found that the number of bacteria can be well decreased. This research showed that using Fe3O4 and CS-Fe3O4 increased NH3-N levels in cultures of free-living nitrogen-fixing bacteria by 15.40% and 75.54%. For future development, it can be in the form of optimization, the effect of adding the same material to plant secondary metabolites, and the mechanism of bacteria in using the material.
Pupuk Organik dari Limbah Padat Kotoran Sapi yang Diperkaya Mikrob Potensial Saprudin, Deden; Farid, Muhammad; Hazra, Fahrizal; Arrahman, Naufal
Jurnal Abdimas Kartika Wijayakusuma Vol 5 No 3 (2024): Jurnal Abdimas Kartika Wijaya Kusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v5i3.679

Abstract

Industri pertanian di Indonesia terus berupaya mengembangkan solusi berkelanjutan, salah satunya dengan penggunaanpupuk organik. Pengelolaan limbah kotoran sapi di Kampung Manglad, Desa Cibodas, Kecamatan Rumpin, Kabupaten Bogor, menjadi salah satu contoh dalam upaya meningkatkan kualitas pertanian melalui program Pemberdayaan Kelompok Masyarakat (PKM) yang didanai Ditjen Dikti Kemdikbudristek. Kelompok Pengolah Limbah (KPL) di Kampung Manglad bekerja sama dengan IPB untuk mengolah kotoran sapi menjadi pupuk organik padat. Program ini dilaksanakan dalam empat tahap, yaitu meningkatkan pemahaman KPL, pelatihan pembuatan pupuk, adopsi teknologi, dan pemasaran produk. Hasil analisis menunjukkan pupuk organik padat yang dihasilkan memenuhi standar kualitas dengan kandungan N, P, dan K yang melebihi batas minimum. Program ini tidak hanya memberikan solusi terhadap masalah lingkungan tetapi juga meningkatkan ekonomi bagi masyarakat sekitar.
Superoxide Anion Radicals Biosensor Based on Protein Extract from Deinococcus Radiodurans Immobilization by Glutaraldehyde Cross-Linked Afifi, Muhammad Ridho; Iswantini, Dyah; Nurhidayat, Novik; Saprudin, Deden
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.153-160

Abstract

A common enzyme for superoxide anion radicals biosensors is superoxide dismutase (SOD). Deinococcus radiodurans protein extract can replace the use of pure SOD enzymes for superoxide anion radicals biosensors. The purpose of this research is to determine the analytical performance of the superoxide anion radicals biosensor with protein extract of Deinococcus radiodurans immobilized on carboxymethylcellulose-gelatin-zeolit (CMC-G-Z) that is crosslinked by glutaraldehyde. The response surface method showed that the optimum condition for the biosensor was pH 7, protein extract concentration of 1075 µg/ml, zeolite concentration of 5 mg/ml, and glutaraldehyde concentration of 0.0042 M. Stability of the biosensor retained 67% of its sensitivity after use for 24 hours. The biosensor exhibits good analytical performance with a linear range from 0.1 – 0.8 mM, a detection limit of 77.84 µM, and a limit of quantification of 259.5 µM with a correlation coefficient of 0.9905.
Peningkatan kesadaran sanitasi masyarakat pedesaan melalui diseminasi pembuatan sabun transparan Farobie, Obie; Saprudin, Deden; Firmansyah, Shaeful
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5 No 1 (2022)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i1.13822

Abstract

Pengembangan suatu wilayah perlu dilakukan dari satuan terkecil, yaitu pembangunan pedesaan. Desa Tonjong merupakan salah satu dari 410 desa di Kabupaten Bogor yang memiliki potensi yang baik di sektor pertanian, namun masyarakatnya masih memiliki kesadaran yang rendah terkait sanitasi. Kegiatan ini bertujuan memberikan informasi dan pengetahuan mengenai pentingnya menjaga hidup bersih serta transfer pengetahuan terkait pembuatan sabun transparan. Kegiatan ini juga bertujuan untuk mengetahui tingkat kesukaan responden terhadap sabun transparan serta mengevaluasi pemahaman warga terkait proses pembuatan sabun transparan. Tahapan pelaksanaan kegiatan meliputi persiapan, pelatihan pembuatan sabun transparan, dan pelaksanaan evaluasi kegiatan. Hasil uji organoleptik terhadap produk sabun transparan menunjukkan bahwa tingkat kesukaan tinggi pada warna, kelembapan, aroma, dan kehalusan namun rendah pada tingkat kemampuan busa. Hasil evaluasi keseluruhan menunjukkan bahwa ada peningkatan pengetahuan dan pemahaman peserta tentang bahan baku pembuatan sabun (dari 30% menjadi 100%), tata cara pembuatan sabun transparan (dari 5% menjadi 100%), serta manfaat sabun untuk kesehatan (dari 5% menjadi 100%).
Effects of Gibberellin on Physical and Chemical Quality of Oil Palm (Elaeis guineensis Jacq.) Fresh Fruit Bunches Ramanis, Imelya; Sudradjat, Sudradjat; Saprudin, Deden
Journal of Tropical Crop Science Vol. 10 No. 02 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.02.87-93

Abstract

The quality of crude palm oil (CPO) is influenced by the quality of fresh fruit bunches, crop culture, and postharvest handling. A delay in fruit processing can cause physical damages to the fresh fruit bunches. Gibberellic acid (GA3) can potentially reduce the physical damage due to delayed processing of the fresh fruit bunches. Our study aims to determine how GA3 affects the physical and chemical quality of oil palm fresh fruit bunches. The fresh fruit bunch samples were collected from the IPB-Cargill Palm Oil Education and Research, Jonggol, Bogor, Indonesia. This study used a randomized complete block design that consisted of four concentrations of GA3: 0, 12.5, 25 and 37.5 ppm. GA3 application reduced fruit loss, respiration rate, and maintain fruit moisture and firmness, increased the oil content, and stabilize the free fatty acid content. GA3 concentration of 12.5 ppm is the optimal concentration. Based on the correlation analysis, fruit softness has a strong correlation with free fatty acids.
CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE Meryandini, Anja; Saprudin, Deden; Prihandono, Prima Agung; Akhdiya, Alina; Hendarwin, Trio
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.57 KB) | DOI: 10.11598/btb.2007.14.2.16

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.
Growth, Photosynthesis and Proline Accumulation of Metal-Accumulator Weeds Hamim, Hamim; Apriani, Rani; Saprudin, Deden; Sulistyaningsih, Yohana C
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.267 KB) | DOI: 10.11598/btb.0.0.0.1200

Abstract

Intensive use of heavy metals, particularly lead and mercury, in industrial activities and mining extraction has caused the widespread distribution of these contaminants, thereby threatening the environment. Consequently, the growth and several physiological responses of five metal-accumulator weed species were examined in response to mercury (Hg) and lead (Pb) treatments. The weed species Brachiaria mutica, Cyperus kyllingia, Ipomoea aquatica, Mikania micrantha, and Paspalum conjugatum were grown in water culture using half-strength Hoagland's solution and subjected to Hg(NO₃)₂ and Pb(NO₃)₂ at concentrations of 0, 0.25, and 0.5 mM for 3 weeks. Plant growth, photosynthesis, lipid peroxidation, and proline content were observed. Both Hg and Pb significantly reduced plant growth, but the decrease was more pronounced under Hg treatment than under Pb. Hg dramatically reduced the photosynthetic rate under different photosynthetic photon flux densities, indicating that Hg up to 0.5 mM damaged the photosynthetic apparatus in almost all species except I. aquatica. Hg and Pb treatments caused a dramatic increase in leaf MDA content, which was associated with a significant decrease in chlorophyll content. Most species were tolerant to Pb up to 0.5 mM except M. micrantha, while only C. kyllingia and I. aquatica were tolerant to Hg treatment up to 0.5 mM. Hg treatment also induced higher proline accumulation in leaves, although without a clear increment pattern among species, suggesting that proline may function as a stress alarm rather than as a tolerance indicator.