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All Journal Bioscience Techno: Jurnal Penelitian JURNAL PengaMAS Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun AMMA : Jurnal Pengabdian Masyarakat
Tundjung Tripeni Handayani
Universitas Lampung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Nursing Social Sciences

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Study of orchid resistance induced by Ceratorhiza sp. against ORSV infection based on peroxidase activity Anggi Anggreiny; Tundjung Tripeni Handayani; Mahfut Mahfut
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152112960-0-00

Abstract

Orchid is one of the popular ornamental plants that widely grown in Indonesia. However, the process of orchid cultivation is often hampered by virus infections. The virus that often infects the orchids is Odontoglossum ringspot virus. Viruses that enter orchid cells and replicate will activate the orchid’s defense response. This defense response is characterized by the increase of peroxidase activity. The peroxidase enzyme works in the formation of lignin to thicken cell walls and prevent viruses from entering other cells. In addition, the orchid defense response can also be activated through induced systemic resistance by inoculation of Ceratorhiza sp. as endophytic mycorrhizae. In this study, Factorial Complete Randomized Design (CRD) was used with 2 factors. Factor 1 is the type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and factor 2 is the type of treatment (inoculation of mycorrhizae, virus, and mycorrhizae-virus). The orchid’s resistance level is determined by the analysis results of peroxidase activity using spectrophotometer. The results obtained indicate that all treatment combinations strongly influence the increase of peroxidase activity. Peroxidase activity of Phalaenopsis amabilis is 1.42 [(Umg) / min] and Dendrobium discolor is 1.64 [(Umg) / min] in average. Peroxidase activity on Dendrobium discolor was higher than on Phalaenopsis amabilis. This indicates that Dendrobium discolor has a higher level of resistance when compared to Phalaenopsis amabilis.Proses budidaya anggrek seringkali terkendala oleh infeksi virus. Adapun virus yang paling banyak menginfeksi anggrek adalah ORSV. Virus yang masuk ke dalam sel anggrek dan bereplikasi akan mengaktifkan respon pertahanan anggrek. Respon pertahanan ini ditandai dengan peningkatan aktivitas peroksidase. Respon pertahanan anggrek juga dapat diaktifkan melalui induksi resistensi sistemik dengan inokulasi Ceratorhiza sp. sebagai mikoriza endofit. Dalam penelitian ini digunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah jenis anggrek (Phalaenopsis amabilis dan Dendrobium discolor) dan faktor kedua adalah jenis perlakuan (inokulasi mikoriza, virus, dan mikoriza virus). Tingkat ketahanan anggrek ditentukan dari hasil analisis aktivitas peroksidase menggunakan spektrofotometer. Hasil yang diperoleh menunjukkan bahwa semua kombinasi perlakuan sangat berpengaruh terhadap peningkatan aktivitas peroksidase. Aktivitas peroksidase Phalaenopsis amabilis rata-rata 1,42 [(U/mg)/ menit] dan Dendrobium discolor 1,64 [(U/mg)/ menit]. Aktivitas peroksidase pada Dendrobium discolor lebih tinggi dibandingkan dengan Phalaenopsis amabilis. Hal ini menunjukkan bahwa Dendrobium discolor memiliki tingkat ketahanan yang lebih tinggi jika dibandingkan dengan Phalaenopsis amabilis.
PEMANFAATAN ONGGOK SEBAGAI PAKAN ALTERNATIF USAHA PETERNAKAN DAN PERIKANAN DI DESA TAMBAH DADI, KECAMATAN PURBOLINGGO, LAMPUNG TIMUR Mahfut - Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih; Eti Ernawiati
JURNAL PengaMAS Vol 3, No 2 (2020)
Publisher : UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/pengamas.v3i2.1827

Abstract

Tambah Dadi adalah sebuah desa yang terletak di Kecamatan Purbolinggo, Lampung Timur dengan salah satu keunggulan pertanian tanaman ubi kayu. Dalam proses pengolahannya, keberadaan limbah onggok yang menumpuk dan menjadi masalah lingkungan belum dimanfaatkan dengan baik oleh warga setempat. Limbah tanaman ini sebenarnya sangat potensial sebagai pakan ternak alternatif dalam usaha peternakan dan pertanian, karena tersedia dalam jumlah yang besar dan murah. Keuntungan lain pemanfaatan onggok yaitu dapat mengurangi dampak pencemaran lingkungan dan menghemat devisa. Meskipun limbah, onggok memiliki kandungan serat yang tinggi sehingga masih mencukupi kebutuhan gizi sebagai pakan alternatif. Berdasarkan hal tersebut, kegiatan Pengabdian kepada Masyarakat “Pemanfaatan Onggok Sebagai Pakan Alternatif Usaha Peternakan dan Perikanan di Desa Tambah Dadi, Kecamatan Purbolinggo, Lampung Timur” ini perlu dilakukan untuk memberikan pengetahuan kepada masyarakat sehingga masyarakat dapat menghemat biaya produksi dan memperoleh tambahan penghasilan.
Variation of response induced by ceratorhiza on Dendrobium discolor and Phalaenopsis amabilis to odontoglossum ringspot virus infection based on disease intensity and plant resistance level Mahfut Mahfut; Mitha valentina treesya Panjaitan; Tundjung Tripeni Handayani; Sri Wahyuningsih
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261114027-0-00

Abstract

The popular cultivated orchid is Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are very important for orchid plants to complete their life cycle. Mycorrhizae are capable of symbiosis with the root system of the host plant which can increase the absorption of these elements. The purpose of this study was to find out the symptoms of infection, the intensity of infectious diseases, the level of resistance to infection. The method used in this study was completely randomized design (CRD) with 2 factors and 4 iterations, the first factor was the type of orchid used Phalaenopsis amabilis (A1) and Dendrobium discolor (A2), and the second factor was the type of administration of mycorrhizal., Virus , and mycorrhizal virus. Observation of the symptoms of the disease is done by looking at the symptoms that appear on the leaves that have been infected with ORSV, while to determine the intensity of the disease and the level of resistance can use a predetermined formula. The results showed that the disease intensity in Phalaenopsis amabilis was more severe than in Dendrobium discolor, which was 40% on each leaf. The level of resistance in Dendrobium discolor is more resistant than Phalaenopsis amabilis, this indicates that Dendrobium discolor on average has a tolerant response. Ceratorhiza could not suppress the intensity of the disease, because from the results obtained, treatment of orchids inoculated with mycorrhiza and virus showed more severe symptoms than treatment only inoculated with virus. Ceratorhiza was unable to increase the resistance of Phalaenopsis amabilis and Dendrobium discolor, this was because treatment of orchids inoculated with mycorrhiza and virus showed a very simple response compared to treatmentonly inoculated with virus.Anggrek populer dibudidayakan adalah Phalaenopsis sp. dan Dendrobium sp. Mikoriza sangat penting bagi tanaman anggrek untuk melengkapi siklus hidupnya. Mikoriza  mampu bersimbiosis dengan sistem akar tanaman inang yang dapat meningkatkan daya serap unsur tersebut. Tujuan penelitian untuk mengetahui gejala infeksi, intensitas penyakit menular, tingkat resistensi terhadap infeksi. Metode penelitian: Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 4 iterasi, faktor pertama adalah jenis anggrek yang digunakan Phalaenopsis amabilis (A1) dan Dendrobium discolor (A2), dan faktor kedua adalah jenis pemberian mikoriza, virus, dan virus mikoriza. Pengamatan gejala penyakit dilakukan dengan melihat gejala yang muncul pada daun yang telah terinfeksi ORSV. Hasil penelitian menunjukkan intensitas penyakit pada Phalaenopsis amabilis lebih parah dibandingkan dengan Dendrobium discolor yaitu 40% pada setiap daun. Tingkat ketahanan pada Dendrobium discolor lebih tahan dibandingkan Phalaenopsis amabilis. Dendrobium discolor memiliki respon yang toleran. Ceratorhiza tidak dapat menekan intensitas penyakit. Pengobatan  anggrek yang diinokulasi dengan mikoriza dan virus menunjukkan gejala yang lebih parah daripada pengobatan yang hanya diinokulasi virus. Ceratorhiza tidak mampu meningkatkan ketahanan terhadap Phalaenopsis amabilis dan Dendrobium discolor, hal ini dikarenakan perlakuan anggrek yang diinokulasi mikoriza dan virus menunjukkan respon yang sangat sederhana dibandingkan dengan perlakuan yang hanya diinokulasi virus.
Study of Resistance on Orchids Induced Rhizoctonia to ORSV Based on Secondary Metabolite Analysis Mahfut Mahfut; Widi Aryani; Tundjung Tripeni Handayani; Sri Wahyuningsih
TECHNO: JURNAL PENELITIAN Vol 11, No 2 (2022): TECHNO JURNAL PENELITIAN
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v11i2.3044

Abstract

Dendrobium discolor and Phalaenopsis amabilis are in high demand because of their beautiful shape, color, texture, and flower arrangement. The current obstacle to orchid cultivation is infection with Odontoglossum ringspot virus (ORSV). One of the efficient ways to control the infection of this pathogen and does not cause an impact on the environment is to use Rhizoctonia mycorrhizae. Mycorrhizal relationship with plants will form induced resistance. Plants resulting from induced resistance will induce secondary metabolite, namely peroxidase enzymes. This type of secondary metabolites will prevent the growth and development of pathogens and plants become resistant to pathogenic infections. The purpose of this study was to determine differences in peroxidase enzyme activity and differences in resistance to P. amabilis and D. discolor after Rhizoctonia induction against ORSV infection. This research was carried out in several stages, namely plantlet preparation, mycorrhizal inoculation, mycorrhizal inoculation, and peroxidase enzyme activity analysis using a UV Vis spectrometer at a wavelength of 420 nm. The results showed that D. discolor had a higher peroxidase enzyme activity than P. amabilis. In the treatment application factor, the peroxidase enzyme activity was the highest in the Mycorrhizal Virus treatment compared to the Mycorrhizal and Virus treatments. This study showed that D. discolor was more resistant to ORSV infection than P. amabilis. and the application of Mycorrhizae proved to be successful in increasing the activity of the peroxidase enzyme as a response to plant protection against viral infections.
Penyuluhan Teknik Perbanyakan Tanaman Hias Sebagai Penunjang Perekonomian Keluarga Eti Ernawiati; Tundjung Tripeni Handayani; Sri Wahyuningsih; Mahfut Mahfut
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 4 No. 2 (2023)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v4i2.113

Abstract

Pemberdayaan ekonomi keluarga merupakan bentuk kepedulian atas permasalahan kemiskinan dan penciptaan lapangan kerja. Ibu-ibu rumah tangga di pedesaan perlu didorong berwirausaha agar dapat menunjang ekonomi keluarga. Wirausaha budidaya tanaman hias cocok bagi ibu-ibu rumah tangga karena berskala rumahan dengan modal kecil. Perbanyakan tanaman merupakan salah satu komponen penting dalam budidaya tanaman hias untuk menjamin ketersediaan bibit berkualitas. Ibu-ibu rumah tangga di Desa Bandar Sari, Kecamatan Padang Ratu, Kabupaten Lampung Tengah sebagian besar berpendidikan rendah sehingga pengetahuan dan keterampilan budidaya tanaman hias mereka terbatas. Oleh karena itu kegiatan ini bertujuan untuk memberikan pengetahuan dan keterampilan teknik perbanyakan tanaman hias. Kegiatan dilaksanakan menggunakan metode ceramah dan demonstrasi. Hasil evaluasi kegiatan yang dilakukan melalui pretest dan posttest menunjukkan peningkatan pemahaman peserta yang cukup signifikan sebesar 76,96 %, yaitu dari nilai rata-rata pretest 48,84 meningkat menjadi 63,46 pada posttest. Sesi diskusi juga berlangsung kondusif dengan pertanyaan-pertanyaan peserta yang menunjukkan keingintahuan yang tinggi untuk memperoleh pengetahuan.
Implementasi Aplikasi Sistem “Pakar Anggrek” Berbasis Android Di Kebun Raya Liwa Mahfut; Admi Syarif; Tundjung Tripeni Handayani; Sri Wahyuningsih; Kurnia Muludi; Muhammad Irfan Ardiansyah
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 7 : Agustus (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Natural orchids are one of the original flora collections in Liwa Botanical Gardens that need to be preserved. Based on the results of our previous research, it is known that some natural orchids show symptoms of disease infection. The Liwa Botanical Gardens do not yet know a safe disease infection control technique. Disease control is still using pesticides that are harmful to the environment. The results of our previous research have resulted in new methods and solutions for disease control through a web-based expert system. This method was considered very helpful for diagnosis but was considered inefficient, so the Android-based "Orchid Expert" application was developed. This Community Service Dissemination of Research Results (PKM-DHR) activity is a continuation of our previous research results. The problem-solving formulation method is carried out through direct counselling and training at the Liwa Botanical Gardens. The activity was carried out on Wednesday, August 16, 2023, with a total of 30 participants. The results of the training activities at the demonstration stage of downloading and using the application show that the accuracy of the system diagnostic results is valid, with an accuracy rate of 92.9%. This can be interpreted as meaning that this application can be applied in society. The results of this activity indicate that the Liwa Botanical Garden management understands and is able to apply the development of natural orchid disease control methods as a conservation effort. Furthermore, the Liwa Botanical Garden is also expected to be able to independently diagnose and control natural orchid disease infections.
Penyuluhan Pemanfaatan Limbah Cair Tapioka Sebagai Alternatif Pembuatan Nata De Cassava Di Kelompok PKK Desa Krawang Sari, Natar, Lampung Selatan Hardoko Insan Qudus; Endang Nurcahyani; Tundjung Tripeni Handayani; Sri Wahyuningsih; Sutyarso
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 8 : September (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The tubers of cassava or cassava (Manihot esculenta Crantz) are widely known as a staple food that produces carbohydrates and the leaves are a vegetable. Indonesia is the second largest cassava producing country after Nigeria with a five-year average total supply of 9.67 million tons. Processed cassava produces two-thirds of the by-products of its raw materials in the form of tapioca cob, skin, and liquid waste. Tapioca liquid waste can pollute the surrounding environment because it produces an unpleasant odor. Based on these reasons, it is necessary to develop new innovations in their utilization, which so far have not been utilized by the community. This new innovation is to use liquid tapioca waste to make Nata de Cassava which is a high fiber and nutritious food. The aim of the service is to provide knowledge to the women of the “PKK” group in Krawang Sari Village, Natar, South Lampung in making Nata de Cassava from liquid tapioca waste. The result of this service activity is that there is an increase in participants' knowledge about how to use liquid tapioca waste to make Nata de Cassava, which can be seen from the average increase in participants' knowledge of 33.33 points and the percentage increase in knowledge of 61.33%.
Co-Authors Admi Syarif Anggi Anggreiny Dian Putri Sani Elsi Diana Endang Nurcahyani Eti Ernawiati Eti Ernawiati Hardoko Insan Qudus Kurnia Muludi Mahfut Martha Lulus Lande Mitha Valentina Treesya Panjaitan Muhammad Irfan Ardiansyah Sri Wahyuningsih Sri Wahyuningsih Sutyarso Widi Aryani zulkifli zulkifli