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All Journal Alchemy : Journal of Chemistry The Journal of Pure and Applied Chemistry Research Journal of Islamic Pharmacy
Diana Candra Dewi
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Exploration of Total Flavonoid Content, Toxicity, and Antibacterial Activity of Acorus calamus L. Rhizome Isolates Amalia, Suci; Jannah, Faridatul; Afriani, Ida Irma; Dewi, Diana Candra; Fasya, A. Ghanaim
The Journal of Pure and Applied Chemistry Research Vol. 14 No. 1 (2025): Edition January-April 2025
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2025.014.01.7914

Abstract

Acorus calamus L. is a medicinal plant with rhizomes commonly used in traditional medicine. This study aimed to determine the total flavonoid content, toxicity, and antibacterial activity of isolates from the rhizome of Acorus calamus L. The rhizome extract was sonicated using 70% ethanol, yielding 18.73% (w/w), and partitioned using ethyl acetate, yielding 10.80% (w/w). Subsequently, the ethyl acetate fraction was further separated using column chromatography. FTIR analysis of the ethanol extract, ethyl acetate fraction, and column isolates confirmed the presence of O–H functional groups and other vibrations, indicating the presence of flavonoid compounds. UV analysis revealed absorption at the band I (302, 302, 383 nm) and band II (253, 254, 258 nm). The total flavonoid content of the isolates, determined using AlCl3, was 32.95 mg QE/g. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis of the column chromatography isolates identified the presence of isorhamnetin, quercetin, genistein, and glycitein. Furthermore, toxicity and antibacterial activity tests on the ethanol extract and ethyl acetate fraction showed LC50 values of 143.53 ppm and 41.16 ppm, respectively, and inhibition zone diameters ranging from 2.30-4.54 mm and 2.63-5.29 mm, respectively.
Co-Authors A. Ghanaim Fasya Afriani, Ida Irma Amalullia, Diana Arief Suryadinata Aulani`am Aulani`am Aulanni`am, Aulanni`am Begum Fauziyah Chanif Mahdi Chanif Mahdi Dewi Annisa, Dewi Eny Rahmawati, Eny Fauziyah, Begum Hermin Sulistyarti Himmatul Barroroh JANNAH, FARIDATUL Kumalawati, Oktrin Rustika Mahmudah, RIf'atul Meyfian, Yusha Hasna Muhammad Afif Muhammad Chalid Al-ayubi, Muhammad Chalid Rahmawati Ningsih, Rahmawati Rahmawati, Eny Siti Nurul Afifah, Siti Nurul Suci Amalia