Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

The POTENTIAL OF LACTIC ACID BACTERIA, ISOLATED FROM SEVERAL SOURCES, TO INHIBIT THE GROWTH OF Candida albicans ATCC10231 Ramona, Yan; Darmayasa, Ida Bagus Gede; Dharmawan, Komang; Sudaryatma, Putu Eka; Teriyani, Ni Made; Batho, Yanuarius Felix; Inabuy, Fainmarinat Selviani
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1868

Abstract

ARTICLE HIGHLIGHTS- Lactic acid bacteria have potential to control candidiasis or Candida albicans. - The novelty of this study is to advance the potential of lactic acid bacteria to control candidiasis infection in human, with a view to develop novel LAB-based probiotic candidates with capability to inhibit/prevent infection by C. albicans, the causative agent of candidiasis.ABSTRACTThe main aims of this research were to isolate and identify potential lactic acid bacteria (LAB) inhibitory to Candida albicans. The LAB sources were kimchi, honey and vaginal secrete of healthy women. They were isolated with a view to develop a novel alternative method with reduced use of antifungal agents in the treatment of patients infected by such fungal pathogen. Isolation of the LABs was conducted by applying dilution spread method on de Mann Rogosa Sharpe agar (MRSA) medium supplemented with bromo cresol purple (BCP) indicator. Once purified, they were tested for antagonism against C. albicans in dual culture assays. LAB isolates that showed significant inhibition against the pathogen were identified using 16s rDNA sequences and their sequences were aligned with those of known sequences deposited at the Gene Bank (http://www.ncbi.nlm.nih.gov). The results showed that 46 among more than 100 LABs isolated in this study significantly inhibited the growth of C. albicans in the in vitro dual culture assays, and all showed resistance property to antifungal agent (fluconazole). This indicated that they all have potential to be synergically applied with reduced use of fluconazole in the therapy. The most potential isolates (10 isolates) were closely related to three LAB species, namely Lactobacillus paracasei, Lacticaseibacillus paracasei, and Pediococcus pentosaceus, based on their 16s rDNA sequence similarities with those deposited in the GenBank.
Co-Authors A.A DWI MARSITA ANGGRAENI AA Sudharmawan, AA ADE AYU NITA DEVI AULIA ATIKA PRAWIBTA SUHARTO Batho, Yanuarius Felix DERY MAULANA DESAK PUTU DEVI DAMIYANTI Desak Putu Eka Nilakusmawati DEVI NANDITA. N DEWA AYU AGUNG PUTRI RATNASARI ELVINA LIADI FEBBY VERENNIKA Fransisca Emmanuella Aryossi G. K Gandhiadi G. K. GANDHIADI Gandhiadi, G K GEDE SUMENDRA HAMITA HAKMI HERLINA HIDAYATI I G. A. Widagda I GEDE ARYA DUTA PRATAMA I GEDE ERY NISCAHYANA I GEDE RENDIAWAN ADI BRATHA I Gusti Ayu Made Srinadi I GUSTI AYU MITA ERMIA SARI I GUSTI MADE AYU ANGGUN TIARA PRATINI I GUSTI PUTU NGURAH MAHAYOGA I KOMANG GDE SUKARSA I KOMANG TRY BAYU MAHENDRA I NYOMAN BRYAN ANDIKA I Nyoman Widana I Putu Eka Nila Kencana I PUTU OKA PARAMARTHA I PUTU YUDHI PRATAMA I Wayan Sumarjaya I WAYAN WIDHI DIRGANTARA ICHA WINDA DIAN SAFIRA IDA AYU EGA RAHAYUNI IDA AYU GDE KHASMANA PUTRI IDA AYU PUTU CANDRA DEWI IDA BAGUS ANGGA DARMAYUDA Ida Bagus Gede Darmayasa IKHSAN AKBAR Inabuy, Fainmarinat selviani INTAN AWYA WAHARIKA INTAN LESTARI IRENE MAYLINDA PANGARIBUAN KADEK FRISCA AYU DEVI KADEK INTAN SARI KADEK MIRA PITRIYANTI Kartika Sari Ketut Jayanegara LUH HENA TERECIA WISMAWAN PUTRI LUH PUTU IDA HARINI Luh Putu Ratna Sundari LUSIA EMITRIANA MAGOL MADE ASIH MAKBUL MUFLIHUNALLAH MERARY SIANIPAR MIRANDA NOVI MARA DEWI N. N. Rupiasi NABILA NUR JANNAH NI KADEK NITA SILVANA SUYASA NI KADEK PUSPITAYANTI Ni Ketut Tari Tastrawati NI LUH NIKASARI NI LUH PUTU KARTIKA WATI Ni Luh Putu Suciptawati Ni Made Asih NI MADE NITA ASTUTI NI NYOMAN AYU ARTANADI Ni Nyoman Rupiasih NI PUTU AYUNDA SURYA DEWI Ni Putu Leony Putri Paramita NI PUTU WIDYA ISWARI DEWI NI WAYAN UCHI YUSHI ARI SUDINA PUTU AMANDA SETIAWANI PUTU AYU DENI Putu Eka Sudaryatma PUTU IKA OKTIYARI LAKSMI PUTU MIRAH PURNAMA D. PUTU SAVITRI DEVI PUTU WIDYA ASTUTI Ratna Sari Widiastuti RISKA YUNITA SAYID QOSIM SORAYA SARAH AFIFAH Surma, Odilia Gratiaplena Susanti Marito Barus Swastika, Putu Veri Teriyani, Ni Made Tjokorda Bagus Oka VIAN RISKA AYUNING TYAS VIKY AMELIAH WAYAN ARTHINI Wijayakusuma, I Gusti Ngurah Lanang WIRYA SEDANA Yan Ramona YOHANA Th.V. SERAN YOSEVA AGUNG PRIHANDINI