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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Inabuy, Fainmarinat selviani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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In vitro antifungal activity of trembesi leaf extract [Samanea saman (Jacq.) Merr.] against Colletotrichum magnum Rossman & Allen, the causal agent of papaya anthracnose Ratnasari, Athalia; Darmadi, Anak Agung Ketut; Inabuy, Fainmarinat selviani; Sudatri, Ni Wayan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225317-327

Abstract

Papaya fruit production in Bali has decreased over the last three years (2020-2022). One of the contributing factors is infection by Colletotrichum spp., which causes anthracnose. Anthracnose is an airborne disease that infects various plant commodities including papaya. The use of chemical fungicides to control anthracnose can have negative impacts if used long-term. Trembesi [Samanea saman (Jacq.) Merr.] extract has previously been reported to possess antifungal, antiseptic, antibacterial, and antidiabetic properties. This study aimed to determine the effectiveness and category of inhibitory activity, Minimum Inhibitory Concentration (MIC), Lethal Concentration 50% (LC??), and the phytochemical content of trembesi leaves. Methanol extract of trembesi leaves effectively inhibited the growth of Colletotrichum magnum at a concentration of 5%. The experiment used 11 treatments with 4 replications, including control (0% v/v) and extract concentrations of 1% to 10% (v/v), along with a positive control. The corresponding inhibition zone diameters were: 0.00 mm, 12.00 mm, 11.87 mm, 13.62 mm, 16.00 mm, 19.62 mm, 15.75 mm, 16.87 mm, 17.87 mm, 17.87 mm, 18.25 mm, and 20.87 mm, respectively. The minimum concentration of extract showing inhibitory activity (MIC) was 0.3%, while the LC?? value was 0.32%. Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, steroids, terpenoids, flavonoids, and saponins in the trembesi leaf extract.
The POTENTIAL OF LACTIC ACID BACTERIA, ISOLATED FROM SEVERAL SOURCES, TO INHIBIT THE GROWTH OF Candida albicans ATCC10231 Ramona, Yan; Darmayasa, Ida Bagus Gede; Dharmawan, Komang; Sudaryatma, Putu Eka; Teriyani, Ni Made; Batho, Yanuarius Felix; Inabuy, Fainmarinat Selviani
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1868

Abstract

ARTICLE HIGHLIGHTS- Lactic acid bacteria have potential to control candidiasis or Candida albicans. - The novelty of this study is to advance the potential of lactic acid bacteria to control candidiasis infection in human, with a view to develop novel LAB-based probiotic candidates with capability to inhibit/prevent infection by C. albicans, the causative agent of candidiasis.ABSTRACTThe main aims of this research were to isolate and identify potential lactic acid bacteria (LAB) inhibitory to Candida albicans. The LAB sources were kimchi, honey and vaginal secrete of healthy women. They were isolated with a view to develop a novel alternative method with reduced use of antifungal agents in the treatment of patients infected by such fungal pathogen. Isolation of the LABs was conducted by applying dilution spread method on de Mann Rogosa Sharpe agar (MRSA) medium supplemented with bromo cresol purple (BCP) indicator. Once purified, they were tested for antagonism against C. albicans in dual culture assays. LAB isolates that showed significant inhibition against the pathogen were identified using 16s rDNA sequences and their sequences were aligned with those of known sequences deposited at the Gene Bank (http://www.ncbi.nlm.nih.gov). The results showed that 46 among more than 100 LABs isolated in this study significantly inhibited the growth of C. albicans in the in vitro dual culture assays, and all showed resistance property to antifungal agent (fluconazole). This indicated that they all have potential to be synergically applied with reduced use of fluconazole in the therapy. The most potential isolates (10 isolates) were closely related to three LAB species, namely Lactobacillus paracasei, Lacticaseibacillus paracasei, and Pediococcus pentosaceus, based on their 16s rDNA sequence similarities with those deposited in the GenBank.
Co-Authors Anak Agung Ketut Darmadi Batho, Yanuarius Felix Ida Bagus Gede Darmayasa Komang Dharmawan Ni Wayan Sudatri Putu Eka Sudaryatma Ratnasari, Athalia Teriyani, Ni Made Yan Ramona