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All Journal Biotropika Tropical Plantation Journal
Ida Bagus Gede Darmayasa
Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Antibacterial Activity Screening Of Bacterial Isolates Associated With Seaweed Eucheuma cottonii From Coastal Area In Buleleng, Bali Purnami, Pande Putu Christine Putri; Indraningrat, Anak Agung Gede; Darmayasa, Ida Bagus Gede
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.07

Abstract

Seaweeds like other marine organisms develop a strong association with prokaryotic communities, especially bacteria. Many of these associated bacteria play an important role in synthesizing secondary metabolites which are beneficial for their host, especially against predation or infection. In Indonesia, Eucheuma cottonii is one of the seaweed species that has been cultivated widely.  However, only limited study has been reported on bacterial isolates and their antibacterial activity from E. cottonii.  Bacterial isolates from E. cottonii were cultivated in six agar media namely Zobell Marine Agar, Nutrient Agar, Plate Count Agar, ISP-1, ISP-2, and Starch-M protein agar. Cultivation of bacterial isolates yielded 23 isolates with nine bacterial isolates were classified as Gram-positive bacteria and 14 isolates were grouped among Gram-negative bacteria. All the bacterial isolates were screened for their antibacterial activity against six bacterial indicator strains namely Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603 using perpendicular streak and agar block method. Six out of 23 bacterial isolates displayed antibacterial activity against at least one of the bacterial indicator strains. The bacterial isolate ISP1RL4 showed the highest antibacterial activity with average inhibition of  > 20 mm against all bacterial indicators. Overall, our result indicated the potential of bacterial isolates associated with E. cottonii as an antibacterial producer.
Antibacterial Activity and Preliminary Phytochemical Screening of Forest Chili Leaf (Piper caninum Bl.) Ethanol Extract against Staphylococcus epidermidis Shanzet, Flavia Fionessa Falenda; Darmayasa, Ida Bagus Gede; Suriani, Ni Luh; Wiadnyani, Anak Agung Istri Sri; Ho, Ting Seng; Rusdianasari, Rusdianasari; Mariani, Mariani
Tropical Plantation Journal Vol 3, No 1 (2024): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v3i1.34

Abstract

Forest chili leaves (Piper caninum Bl.) has a potential to become an alternative to conventional antibiotics. This study aims to determine the Minimum Inhibitory Concentration (MIC) of forest chili leaf extract, assess the Lethal Concentration (LC50), and analyze the phytochemical composition of the extract. This study also conducted a Completely Randomized Design (CRD) with four repetitions. The agar well diffusion method is used in this study with various concentrations such as T1 ethanol 96% (negative control), T2 (5% concentration), T3 (10% concentration), T4 (15% concentration), T5 (20% concentration), T6 (25% concentration), and T7 Ciprofloxacin 1% (positive control). Data were subjected to ANOVA at a significance level of 5% and further analyzed using Duncan's test. The results showed that all concentrations of forest chili leaf extract (P. caninum Bl.) exerted a statistically significant effect (P 0.05) against Staphylococcus epidermidis bacteria. The research found that the most effective concentration to inhibits S. epidermidis is at 25%, which generates an inhibitory zone of 25.38 ± 0.52 mm. The estimated MIC value for forest chili leaf extract against S. epidermidis was found to be 0.2%, producing an inhibitory zone of 6.38 ± 0.32 mm. Additionally, the calculated LC50 value was 0.26%. Phytochemical screening revealed the presence of various bioactive compound groups in the forest chili leaf extract, including alkaloids, flavonoids, phenols, tannins, and steroids.
Co-Authors Anak Agung Istri Sri Wiadnyani Ho, Ting Seng Indraningrat, Anak Agung Gede Mariani Mariani Ni Luh Suriani Purnami, Pande Putu Christine Putri Rusdianasari Rusdianasari Shanzet, Flavia Fionessa Falenda