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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Jurnal Sumberdaya Hayati Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Biologi Tropis Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Jamu

Diah Ratnadewi

Biology Department, Fac. Mathematics And Natural Sciences, IPB University

Author-ID : 253482

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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GFDD4-1 Gene Expression in Physcomitrella patens and Homologous Gene in Arabidopsis thaliana in Response to Abiotic Stress DIAH RATNADEWI; WOLFGANG FRANK
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

A number of abiotic stress responsive genes have been identified from various plant species through reverse genetic strategy. A group of genes are involved in plant responses to stress; they are activated by diverse stress conditions and through different mechanisms. One single gene can be induced by several different stress factors; on the other hand, a number of genes can be up-regulated by a single factor. In Physcomitrella patens, through Northern hybridization, the transcript level of the gene GFDD4-I was detected to be markedly increased by ABA, dehydration and cold, but not by salinity and osmotic stress. In Arabidopsis thaliana, a homologous gene to GFDD4-1 namely At2g47770, was confirmed to fulfill similar function as in P. patens: it is inducible by various abiotic stress treatments, i.e. ABA, dehydration, salinity, and cold. Inducible genes in response to abiotic stress factors may be responsible for plant tolerance to those factors.

Effect of Carbohydrate Source on Growth and Performance of In Vitro Sago Palm (Metroxylon sagu Rottb.) Plantlets . SUMARYONO; WIRDHATUL MUSLIHATIN; DIAH RATNADEWI
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Sago palm (Metroxylon sagu Rottb.), grown mostly in the tropics, is one of the most productive carbohydrate-producing crops. However, it is still underutilized. Tissue culture of sago through somatic embryogenesis has been developed. The plantlets derived from somatic embryos, however, are usually weak with few leaves and roots and have low survival rates during acclimatization. Carbohydrate is commonly added into culture medium as an energy source and an osmotic agent. Research was conducted to determine a suitable carbohydrate for plantlets growth in order to produce vigorous plantlets of sago. The basal medium used was a modified MS medium with a half-strength of salts. Different types of carbohydrate (sucrose, maltose, glucose, and fructose) at various concentrations (30, 45, and 60 g/l) were added into the medium. A single 2 cm plantlet derived from somatic embryo was cultured on a culture tube. Each treatment consisted of 15 plantlets. The cultures were incubated in a culture room with light intensity at 20 mmol/m2/s and temperature at 26 oC. The results show that different types and concentrations of carbohydrate influenced the growth of sago plantlets significantly, but there was no interaction between the two factors. Sucrose was better than other types of carbohydrate, and the concentration of 30 g/l was better than concentrations of 45 or 60 g/l for the growth and vigor of sago plantlets. Medium with a sucrose level at 30 g/l gave the best performance of sago plantlets based on plantlet height, leaf number, biomass fresh weight, stem diameter, and rooting percentage.

Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells Dian Rahma Pratiwi; Yohana Caecilia Sulistyaningsih; Diah Ratnadewi
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Cinchona ledgeriana produces several secondary metabolites. The main quinoline alkaloid, quinine that is widely used as an antimalarial drug, is most commonly extracted from the bark of Cinchona, and its leaves contain several other metabolites. Many studies have revealed that cell culture of Cinchona also produces quinine. Nevertheless, the sites of secondary metabolites accumulation are still elusive. This study is aimed at describing specific anatomical structures where alkaloids and some other secondary metabolites are accumulated as well as their localization in leaves and barks of C. ledgeriana, compared to those found in cultured cells. Fresh leaves and barks, and cells of C. ledgeriana were used for anatomical observation and histochemical tests. It was found that these plant parts have specialized structures, idioblast cells with elliptical- and spherical-shapes, scattered in leaf hypodermis, stem cortex, and secondary phloem. Unspecialized structures such as epidermis and palisade mesophyll tissues were also found accumulating some metabolites. Histochemical tests showed that bark and leaves contained alkaloids, terpenoids, phenolic, and lipophilic compounds. Cultured cells presented positive results for alkaloids and terpenoids.

Isolation of Native Plant-Derived Exosome-like Nanoparticles and Their Uptake by Human Cells Diah Ratnadewi; Christofora Hanny Widjaja; Anggraini Barlian; Rizka Musdalifah Amsar; Ika Dewi Ana; Atik Choirul Hidajah; Hari Basuki Notobroto; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.182-192

The exosome is a nano-sized vesicular particle commonly secreted from eukaryotic cells to extracellular space. Exosome functions in cellular communication, nutrients or bioactive compounds delivery, and cellular immunity. It is believed that plant-derived exosome-like particle (PDEN) potential to deliver nano-bioactive compounds to the human body. As Indonesia is one of the herbal centers of the world, we are encouraged to develop natural richness for human welfare. The study aimed to verify various methods to isolate exosomes from the fresh ginger rhizome and quina cells and examine the ginger PDENs' capacity to internalize in vitro human cells. The PDENs isolation was carried out by centrifugation and precipitation. The particles were observed through a transmission electron microscope, dynamic light scattering, and particle size analysis. Differential ultracentrifugation combined with PEG6000 provided a better size range of particles. Nanoparticles tracking analysis performed the concentration of particles within a specific size range. Ginger and quina PDENs size ranged from 40-650 nm, and the concentration was 3.6 × 107 and 2.8 × 106, respectively. The PKH67-labelled ginger PDENs were taken up by human dermal fibroblast and human Wharton's jelly mesenchymal stem cells after 30 minutes and 21 hours. DPPH assays revealed that ginger PDENs have antioxidant activity, indicating the presence of some nano-molecules in those particles. This study would be the baseline for developing ginger PDENs as a functional food with nano-ingredients, or as an effective vehicle of nano-drug, for human health.

Morphological and Anatomical Comparison between Tetraploid Stevia rebaudiana (Bertoni) Bertoni and its Parental Diploid Rifatul Adabiyah; Diah Ratnadewi; Tri Muji Ermayanti; Erwin Al Hafiizh; Elsa May Susanti
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.321-335

Stevia rebaudiana (Bertoni) Bertoni is a perennial herbaceous plant that produces natural low glycemic-index sweeteners alternative to sugar cane. The genetic improvement of Stevia needs to be investigated to increase its productivity. Although this plant has a narrow genetic diversity, genetic improvement through polyploidization may succeed. Meanwhile, genotypic characters of tetraploid Stevia were previously reported. This study then observed the phenotypic characters of the tetraploid plants, which aimed to evaluate the morphological and anatomical characteristics of tetraploid Stevia clones compared to its wild-type parental diploid plant. Three tetraploids and one diploid clone were grown in the field for 12 weeks. The results of qualitative descriptive characters showed that the tetraploid clones B60.3H8, P1T22 and P3T5 had habitus type, shoot bud shape, leaf margin, leaf venation, shape, and type of stem, type of flower, and seed similar to the diploid parental clone. However, the quantitative characters showed that the tetraploid clones had leaf size, stem diameter, root size, length of the single flower, length of the flower stalk, and length of fruit which were more extensive than those of the parental diploid clone. The leaf color of tetraploids was a darker green, and the range of initial flowering times was earlier. This finding gives more understanding of the phenotypic and anatomical characteristics of Stevia tetraploids compared to their parental diploid plant.

Plant-Derived Exosome-like Nanoparticles from Emprit Ginger (Zingiber officinale var. Amarum) and Its Potential Metabolite as Functional Food Ingredients Rukmi, Paramita Sadha Dayinta; Ratnadewi, Diah; Wijaya, Christofora Hanny
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.181-195

The Plant-Derived Exosome-like Nanoparticles (PDENs) are nano-sized vesicles secreted by plants that carry various bioactive compounds and have shown promise for use in functional food applications. This study investigated PDENs isolated from emprit ginger (Zingiber officinale var. Amarum), a local ginger variety renowned for its health-promoting properties. PDENs were extracted from rhizomes harvested at 8, 10, and 12 months, and analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. The best-performing sample based on these parameters was selected for further metabolite profiling using LC-QTOF-MS. A total of 41 compounds were identified from the selected GDEN and ginger extract samples—32 compounds in the GDEN and 24 in the extract. These compounds belong to various groups, including amino acids, flavonoids, phenolics, lipids, terpenoids, vitamins, and others. Compound identification was based on public databases and literature concerning their potential as functional food ingredients. Among these, amino acids were the most abundant group in the GDENs, whereas phenolics were the dominant group in the ginger extract. This study underscores the potential of ginger-derived exosome nanoparticles as a rich source of bioactive compounds, supporting their further exploration and application in the development of functional food products. The balanced metabolite profile observed in GDENs highlights their unique advantages over conventional extracts. These findings reinforce the potential of emprit GDENs as promising candidates for functional food development.

Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Amallia, Nur Anggraini Barlian Aris Tjahjoleksono Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Dyah Retno Wulandari Edhi Sandra Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Gustania, Leolita Hadi SUNARSO Hari Basuki Notobroto Hasibuan, Yustiny Andaliza Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Mas’ud, Zainal Alim Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Nurmuliayanti Muis Nur`Aeni, Fitri Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rizka Musdalifah Amsar Rukmi, Paramita Sadha Dayinta Rupa, Darius RUSMILAH SUSENO Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Sulistyaningsih, Yohana Caecilia Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Tjitrosoedirdjo, Soekisman Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud

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