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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan Nasional
Sani, Lalu M. Iqbal
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Earth & Planetary Sciences Environmental Science

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Eksplorasi Biodiversitas Mikroalga Epifit di Perairan Teluk Hurun, Lampung, Menggunakan Analisa Morfologi dan Metode Metabarkoding Widiarti, Riani; Hendrayanti, Dian; Humaida, Milka; Sani, Lalu M. Iqbal
Jurnal Kelautan Nasional Vol 19, No 2 (2024): Agustus
Publisher : Pusat Riset Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkn.v19i2.14509

Abstract

Penelitian mengenai kelimpahan mikroalga epifit di perairan Teluk Hurun belum pernah dilakukan, padahal beberapa mikroalga epifit berpotensi menimbulkan Harmful Algal Blooms. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan menganalisis kelimpahan mikroalga epifit pada makroalga Padina di perairan Teluk Hurun, terutama untuk spesies yang berpotensi menyebabkan HAB sehingga memungkinkan dilakukannya upaya mitigasi. Makroalga Padina diambil secara acak dari kawasan rataan terumbu, dimasukkan ke dalam botol sampel, dan dikocok kuat selama 1 menit. Sampel air disaring menggunakan saringan bertingkat, untuk kemudian diawetkan dan diamati di bawah mikroskop. Analisis eDNA juga dilakukan dengan menyaring sampel air tersebut melalui kertas millipore berukuran pori 0,45 µm menggunakan filtering set, kemudian diamplifikasi dengan set primer 18s v9, dan dilakukan pengurutan basa DNA melalui Illumina iSeq. Hasil morfogenetik menunjukkan bahwa ditemukan mikroalga dari kelompok Bacillariophycea dengan kelimpahan dan proporsi keberadaan tertinggi yaitu Nitzschia dan Navicula. Meskipun kelompok Dinophycea tidak ditemukan pada pengamatan morfologi, namun analisis eDNA menunjukkan 2 spesies yang berpotensi menyebabkan HAB, yaitu Gambierdiscus sp. dan Prorocentrum mexicanum. Sel-sel dari kelompok Dinophyceae mungkin mengalami kerusakan selama proses perlakuan atau pengawetan, namun materi genetik yang terlarut dalam sampel air dapat terdeteksi menggunakan analisis eDNA.
Challenges in Molecular and Morphological Identification of Sponge Species in Raja Ampat Aisyah, Siti Zanuba; Zamani, Neviaty Putri; Cahyani, Ni Kadek Dita; Elfahmi, Elfahmi; Syafrizayanti, Syafrizayanti; Andriani, Yosie; Arafat, Dondy; Bashari, Muhammad Hasan; Hanif, Novriyandi; Sani, Lalu M. Iqbal; Ayu, Inna Puspa; Akbar, Nebuchadnezzar; Subhan, Beginer
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 1 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.1.115-125

Abstract

Sponges (Phylum Porifera) are a group of highly diverse, sessile, and filter-feeding basal metazoans, except spongillids. The majority of sponges are marine species that play an important role in benthic habitat by maintaining the stability of marine ecosystems through nutrient cycling, habitat provision, and bioerosion. However, marine sponges are not easily identifiable because of their lack of species-level distinctive morphological features, which limits efforts to monitor actual species biodiversity. Indonesia is home to approximately 850 identified species of marine sponges, and Raja Ampat archipelago of West Papua province is known for its exceptional marine biodiversity. Despite the species abundance, the exact number of sponges in the region is not well-documented due to the lack of specific studies providing comprehensive data on their diversity. Therefore, this study aimed to conduct a DNA barcoding analysis using the mitochondrial cytochrome oxidase I (COI) gene as a marker, combined with morphological analyses of 22 individuals collected in the waters of Waigeo, Mansuar, and Gam Islands in Raja Ampat. The results showed that 3 samples were identified at the species level (Halichondria sp. and Stylissa carteri) with good query cover and percent identity. This showed the possible presence of undescribed or cryptic species, suggesting a severe lack of reference data for both morphology and molecular analyses of marine sponges in the region. Consequently, the analysis showed the presence of a significant gap in the understanding of sponge biodiversity in Raja Ampat's waters.
Co-Authors Aisyah, Siti Zanuba Ayu, Inna Puspa Bashari, Muhammad Hasan Beginer Subhan Dian Hendrayanti Dondy Arafat Elfahmi Elfahmi, Elfahmi Humaida, Milka Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Novriyandi Hanif Riani Widiarti Syafrizayanti, Syafrizayanti Yosie Andriani